Streptomyces sp. 846.5

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7726 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R7MK27|A0A4R7MK27_9ACTN Uncharacterized protein OS=Streptomyces sp. 846.5 OX=2485156 GN=EDD99_0926 PE=4 SV=1
MM1 pKa = 7.62SSQDD5 pKa = 3.21EE6 pKa = 4.24PLEE9 pKa = 3.97VWIDD13 pKa = 3.44QDD15 pKa = 3.99LCTGDD20 pKa = 4.65GICVQYY26 pKa = 10.96APEE29 pKa = 4.18VFEE32 pKa = 5.9LDD34 pKa = 3.05IDD36 pKa = 3.65GLAYY40 pKa = 10.75VKK42 pKa = 10.93GDD44 pKa = 3.66DD45 pKa = 4.54QEE47 pKa = 5.72LRR49 pKa = 11.84DD50 pKa = 4.1APGATVPVPLTLLQDD65 pKa = 3.88VVDD68 pKa = 4.41SAKK71 pKa = 10.25EE72 pKa = 3.92CPGDD76 pKa = 4.17CIHH79 pKa = 6.42VRR81 pKa = 11.84RR82 pKa = 11.84SRR84 pKa = 11.84DD85 pKa = 3.24LVEE88 pKa = 4.83VYY90 pKa = 10.83GPDD93 pKa = 3.24AVV95 pKa = 3.58

Molecular weight: 10.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V3F0E5|A0A4V3F0E5_9ACTN Corrinoid adenosyltransferase OS=Streptomyces sp. 846.5 OX=2485156 GN=EDD99_2604 PE=3 SV=1
MM1 pKa = 8.03RR2 pKa = 11.84ARR4 pKa = 11.84SSWPSSKK11 pKa = 10.81SSVSSYY17 pKa = 8.16VRR19 pKa = 11.84RR20 pKa = 11.84PRR22 pKa = 11.84LSRR25 pKa = 11.84RR26 pKa = 11.84LSYY29 pKa = 10.52EE30 pKa = 3.57SWKK33 pKa = 10.36LPSVGPVPPRR43 pKa = 11.84SPRR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84ASRR51 pKa = 11.84HH52 pKa = 5.01PPSPRR57 pKa = 11.84RR58 pKa = 11.84PRR60 pKa = 11.84PPRR63 pKa = 11.84HH64 pKa = 6.01PVRR67 pKa = 11.84ADD69 pKa = 3.52RR70 pKa = 11.84APQRR74 pKa = 11.84RR75 pKa = 11.84ALPPPLLRR83 pKa = 11.84PPRR86 pKa = 11.84RR87 pKa = 11.84RR88 pKa = 11.84PRR90 pKa = 11.84LPPRR94 pKa = 11.84RR95 pKa = 11.84VPPLPRR101 pKa = 11.84VRR103 pKa = 11.84VPAPPRR109 pKa = 11.84RR110 pKa = 11.84ARR112 pKa = 11.84SPRR115 pKa = 11.84RR116 pKa = 11.84PSSRR120 pKa = 11.84PRR122 pKa = 11.84PRR124 pKa = 11.84PPPRR128 pKa = 11.84RR129 pKa = 11.84RR130 pKa = 11.84RR131 pKa = 11.84LPPRR135 pKa = 11.84APLPQVRR142 pKa = 11.84APAPPRR148 pKa = 11.84RR149 pKa = 11.84VPPRR153 pKa = 11.84PRR155 pKa = 11.84APVRR159 pKa = 11.84PVRR162 pKa = 11.84VRR164 pKa = 11.84ATTPSRR170 pKa = 11.84PATPACRR177 pKa = 11.84APAPVRR183 pKa = 11.84PAPAPRR189 pKa = 11.84GPVATVRR196 pKa = 11.84LRR198 pKa = 11.84VATVRR203 pKa = 11.84PARR206 pKa = 11.84VATVRR211 pKa = 11.84PVPRR215 pKa = 11.84APVATVRR222 pKa = 11.84PVPAVTVRR230 pKa = 11.84LPVARR235 pKa = 11.84PAPVATVRR243 pKa = 11.84PVRR246 pKa = 11.84AVTAPAVPRR255 pKa = 11.84VRR257 pKa = 11.84VATVPALPAGCPVRR271 pKa = 11.84RR272 pKa = 11.84AVPAPAARR280 pKa = 11.84ARR282 pKa = 11.84PVCRR286 pKa = 11.84VPTRR290 pKa = 11.84AA291 pKa = 3.83

Molecular weight: 32.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7726

0

7726

2609667

29

8166

337.8

35.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.835 ± 0.043

0.797 ± 0.008

5.595 ± 0.023

5.166 ± 0.033

2.736 ± 0.015

9.403 ± 0.032

2.214 ± 0.011

3.263 ± 0.022

1.83 ± 0.021

10.675 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.718 ± 0.011

1.95 ± 0.019

6.016 ± 0.028

3.183 ± 0.019

7.548 ± 0.041

5.685 ± 0.029

6.33 ± 0.035

8.428 ± 0.03

1.526 ± 0.013

2.102 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski