Streptococcus satellite phage Javan292
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 20 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZIW7|A0A4D5ZIW7_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan292 OX=2558611 GN=JavanS292_0002 PE=4 SV=1
MM1 pKa = 6.5 TTEE4 pKa = 4.22 QIADD8 pKa = 3.59 NYY10 pKa = 10.85 EE11 pKa = 4.22 SLTSDD16 pKa = 2.56 MMTAVSALDD25 pKa = 3.29 IVRR28 pKa = 11.84 RR29 pKa = 11.84 WLEE32 pKa = 3.67 NAKK35 pKa = 10.54 KK36 pKa = 10.26 SDD38 pKa = 2.97 EE39 pKa = 4.31 GYY41 pKa = 11.05 YY42 pKa = 11.05 GFIALKK48 pKa = 9.98 EE49 pKa = 4.25 SYY51 pKa = 11.53 ANIINLAMNDD61 pKa = 3.86 LQRR64 pKa = 11.84 LASEE68 pKa = 4.35 HH69 pKa = 5.77 YY70 pKa = 10.26 KK71 pKa = 10.45 IIEE74 pKa = 4.13 EE75 pKa = 4.42 VIKK78 pKa = 10.43 EE79 pKa = 4.0
Molecular weight: 9.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.607
IPC2_protein 4.66
IPC_protein 4.495
Toseland 4.355
ProMoST 4.507
Dawson 4.431
Bjellqvist 4.647
Wikipedia 4.291
Rodwell 4.329
Grimsley 4.266
Solomon 4.418
Lehninger 4.38
Nozaki 4.546
DTASelect 4.66
Thurlkill 4.355
EMBOSS 4.304
Sillero 4.596
Patrickios 3.757
IPC_peptide 4.431
IPC2_peptide 4.596
IPC2.peptide.svr19 4.545
Protein with the highest isoelectric point:
>tr|A0A4D5ZN13|A0A4D5ZN13_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan292 OX=2558611 GN=JavanS292_0005 PE=4 SV=1
MM1 pKa = 7.53 NIKK4 pKa = 9.4 EE5 pKa = 4.28 VKK7 pKa = 9.53 KK8 pKa = 10.38 KK9 pKa = 10.5 NGSIVYY15 pKa = 9.6 RR16 pKa = 11.84 ASIYY20 pKa = 10.81 LGIDD24 pKa = 2.92 SLTGKK29 pKa = 10.14 KK30 pKa = 10.06 VRR32 pKa = 11.84 TTVTAPTKK40 pKa = 10.56 KK41 pKa = 10.2 GVQKK45 pKa = 9.45 KK46 pKa = 7.84 TKK48 pKa = 9.93 LAIHH52 pKa = 6.33 NFLTNGATVAEE63 pKa = 4.47 TVSIEE68 pKa = 4.36 TYY70 pKa = 10.89 DD71 pKa = 3.74 EE72 pKa = 5.23 LVSLWWDD79 pKa = 3.37 SYY81 pKa = 11.63 KK82 pKa = 10.04 YY83 pKa = 8.62 TVKK86 pKa = 10.73 EE87 pKa = 3.78 NTRR90 pKa = 11.84 QDD92 pKa = 3.09 MKK94 pKa = 11.29 GYY96 pKa = 9.0 LKK98 pKa = 10.68 KK99 pKa = 10.13 HH100 pKa = 5.53 IRR102 pKa = 11.84 PAFGKK107 pKa = 10.51 YY108 pKa = 9.15 KK109 pKa = 10.17 LKK111 pKa = 10.77 KK112 pKa = 7.82 ITTPIIQRR120 pKa = 11.84 QVNQWANAANKK131 pKa = 10.32 GEE133 pKa = 4.32 AGAFADD139 pKa = 3.73 YY140 pKa = 11.11 HH141 pKa = 6.28 FLHH144 pKa = 6.5 AVNRR148 pKa = 11.84 RR149 pKa = 11.84 ILQYY153 pKa = 10.56 GVAMQALPSNPARR166 pKa = 11.84 DD167 pKa = 3.5 VLVPRR172 pKa = 11.84 KK173 pKa = 9.79 APRR176 pKa = 11.84 DD177 pKa = 3.71 KK178 pKa = 11.37 EE179 pKa = 4.3 PIKK182 pKa = 11.07 YY183 pKa = 10.41 LDD185 pKa = 4.39 DD186 pKa = 4.21 EE187 pKa = 4.38 NLKK190 pKa = 10.76 KK191 pKa = 10.65 FLVYY195 pKa = 10.47 LDD197 pKa = 4.66 SLPNTYY203 pKa = 10.51 QNNFDD208 pKa = 3.67 TVLYY212 pKa = 10.33 KK213 pKa = 10.99 LLLATGLRR221 pKa = 11.84 ISEE224 pKa = 4.15 ALALEE229 pKa = 4.31 WSDD232 pKa = 5.18 IDD234 pKa = 4.01 LQQATLEE241 pKa = 4.3 VNKK244 pKa = 9.47 TLTRR248 pKa = 11.84 SRR250 pKa = 11.84 SVNSPKK256 pKa = 10.7 SKK258 pKa = 8.71 TSNRR262 pKa = 11.84 ILDD265 pKa = 3.93 LDD267 pKa = 3.84 KK268 pKa = 10.91 KK269 pKa = 8.19 TVLMLRR275 pKa = 11.84 LYY277 pKa = 10.89 KK278 pKa = 10.41 NRR280 pKa = 11.84 QQEE283 pKa = 4.0 KK284 pKa = 9.59 ARR286 pKa = 11.84 EE287 pKa = 3.99 IGATPTVVFSKK298 pKa = 9.65 LTSKK302 pKa = 11.07 YY303 pKa = 8.91 MPQNTALLRR312 pKa = 11.84 LKK314 pKa = 9.75 QHH316 pKa = 6.18 IKK318 pKa = 10.25 SAGVTDD324 pKa = 3.74 IAFHH328 pKa = 6.18 GFRR331 pKa = 11.84 HH332 pKa = 4.97 THH334 pKa = 6.74 ASLLLNAGLPYY345 pKa = 10.31 KK346 pKa = 10.05 EE347 pKa = 4.02 LQHH350 pKa = 6.7 RR351 pKa = 11.84 LGHH354 pKa = 6.37 ASISMTMDD362 pKa = 3.16 IYY364 pKa = 11.65 SHH366 pKa = 6.96 LSKK369 pKa = 11.33 EE370 pKa = 4.32 NMKK373 pKa = 10.38 KK374 pKa = 9.99 AASVFEE380 pKa = 4.17 TALDD384 pKa = 3.81 SLTGG388 pKa = 3.55
Molecular weight: 43.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.075
IPC2_protein 9.385
IPC_protein 9.297
Toseland 10.131
ProMoST 9.736
Dawson 10.292
Bjellqvist 9.926
Wikipedia 10.438
Rodwell 10.891
Grimsley 10.35
Solomon 10.306
Lehninger 10.277
Nozaki 10.087
DTASelect 9.926
Thurlkill 10.16
EMBOSS 10.526
Sillero 10.204
Patrickios 10.526
IPC_peptide 10.321
IPC2_peptide 8.346
IPC2.peptide.svr19 8.402
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
20
0
20
3272
63
474
163.6
18.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.518 ± 0.538
0.275 ± 0.117
5.96 ± 0.383
8.374 ± 0.697
3.362 ± 0.316
5.165 ± 0.399
2.292 ± 0.258
6.968 ± 0.403
8.68 ± 0.485
10.116 ± 0.655
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.773 ± 0.268
4.615 ± 0.377
3.087 ± 0.368
4.493 ± 0.35
4.982 ± 0.409
5.165 ± 0.355
5.837 ± 0.473
5.501 ± 0.35
1.039 ± 0.186
4.798 ± 0.292
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here