Escherichia phage HK578

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Dhillonvirus; Escherichia virus HK578

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K7P6Z7|K7P6Z7_9CAUD Uncharacterized protein OS=Escherichia phage HK578 OX=1147142 GN=HK578_056 PE=4 SV=1
MM1 pKa = 7.58CVLDD5 pKa = 3.93YY6 pKa = 10.01EE7 pKa = 4.63QNGIATVCDD16 pKa = 4.14GDD18 pKa = 3.95TKK20 pKa = 10.76TVDD23 pKa = 3.65EE24 pKa = 4.82IEE26 pKa = 4.27EE27 pKa = 4.46TEE29 pKa = 3.98QQ30 pKa = 3.81

Molecular weight: 3.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K7P819|K7P819_9CAUD Uncharacterized protein OS=Escherichia phage HK578 OX=1147142 GN=HK578_055 PE=4 SV=1
MM1 pKa = 7.83DD2 pKa = 4.16RR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84AKK7 pKa = 9.88QLSWHH12 pKa = 6.65IPLHH16 pKa = 6.72CIRR19 pKa = 11.84IWANNAIEE27 pKa = 4.39GDD29 pKa = 4.19SVFWSYY35 pKa = 11.68RR36 pKa = 11.84DD37 pKa = 3.53DD38 pKa = 4.14VKK40 pKa = 11.08PLTQTRR46 pKa = 11.84YY47 pKa = 8.38LHH49 pKa = 6.15QFAAQHH55 pKa = 6.04DD56 pKa = 4.73FYY58 pKa = 11.65VRR60 pKa = 11.84TSLQPMGIRR69 pKa = 11.84ITLRR73 pKa = 11.84RR74 pKa = 11.84KK75 pKa = 8.89PAVEE79 pKa = 3.46RR80 pKa = 11.84VGEE83 pKa = 3.96HH84 pKa = 3.75WRR86 pKa = 11.84LRR88 pKa = 11.84KK89 pKa = 9.52

Molecular weight: 10.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

13620

30

1150

227.0

25.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.382 ± 0.487

1.131 ± 0.146

6.373 ± 0.245

6.138 ± 0.281

3.656 ± 0.181

8.025 ± 0.344

1.865 ± 0.158

5.404 ± 0.258

6.028 ± 0.376

7.313 ± 0.229

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.739 ± 0.181

4.236 ± 0.231

3.899 ± 0.252

3.774 ± 0.276

6.065 ± 0.278

4.794 ± 0.276

6.175 ± 0.279

7.416 ± 0.29

1.645 ± 0.142

2.944 ± 0.173
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski