Amino acid dipepetide frequency for Shewanella sp. phage 1/41

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.658AlaAla: 8.658 ± 1.54
0.954AlaCys: 0.954 ± 0.302
4.696AlaAsp: 4.696 ± 0.621
6.457AlaGlu: 6.457 ± 1.124
2.715AlaPhe: 2.715 ± 0.344
6.09AlaGly: 6.09 ± 0.705
1.467AlaHis: 1.467 ± 0.472
6.457AlaIle: 6.457 ± 0.779
5.87AlaLys: 5.87 ± 0.696
7.264AlaLeu: 7.264 ± 0.773
3.082AlaMet: 3.082 ± 0.54
4.182AlaAsn: 4.182 ± 0.549
2.348AlaPro: 2.348 ± 0.408
2.788AlaGln: 2.788 ± 0.441
2.788AlaArg: 2.788 ± 0.403
5.65AlaSer: 5.65 ± 0.558
5.209AlaThr: 5.209 ± 0.786
7.191AlaVal: 7.191 ± 0.742
1.174AlaTrp: 1.174 ± 0.297
3.008AlaTyr: 3.008 ± 0.45
0.0AlaXaa: 0.0 ± 0.0
Cys
0.66CysAla: 0.66 ± 0.204
0.293CysCys: 0.293 ± 0.118
0.66CysAsp: 0.66 ± 0.199
0.88CysGlu: 0.88 ± 0.233
0.514CysPhe: 0.514 ± 0.167
1.101CysGly: 1.101 ± 0.305
0.293CysHis: 0.293 ± 0.164
0.807CysIle: 0.807 ± 0.247
0.807CysLys: 0.807 ± 0.267
0.734CysLeu: 0.734 ± 0.341
0.367CysMet: 0.367 ± 0.158
0.807CysAsn: 0.807 ± 0.258
0.22CysPro: 0.22 ± 0.122
0.44CysGln: 0.44 ± 0.175
0.66CysArg: 0.66 ± 0.193
0.734CysSer: 0.734 ± 0.258
0.293CysThr: 0.293 ± 0.127
0.66CysVal: 0.66 ± 0.184
0.073CysTrp: 0.073 ± 0.07
0.367CysTyr: 0.367 ± 0.144
0.0CysXaa: 0.0 ± 0.0
Asp
5.43AspAla: 5.43 ± 0.616
0.587AspCys: 0.587 ± 0.159
4.329AspAsp: 4.329 ± 0.712
4.989AspGlu: 4.989 ± 0.63
2.641AspPhe: 2.641 ± 0.51
5.356AspGly: 5.356 ± 0.59
1.027AspHis: 1.027 ± 0.381
5.503AspIle: 5.503 ± 0.563
4.843AspLys: 4.843 ± 0.832
4.476AspLeu: 4.476 ± 0.727
1.467AspMet: 1.467 ± 0.324
3.082AspAsn: 3.082 ± 0.456
1.981AspPro: 1.981 ± 0.32
1.541AspGln: 1.541 ± 0.313
2.275AspArg: 2.275 ± 0.58
4.476AspSer: 4.476 ± 0.603
3.449AspThr: 3.449 ± 0.573
3.962AspVal: 3.962 ± 0.512
0.88AspTrp: 0.88 ± 0.358
2.275AspTyr: 2.275 ± 0.43
0.0AspXaa: 0.0 ± 0.0
Glu
5.503GluAla: 5.503 ± 0.63
1.247GluCys: 1.247 ± 0.324
4.622GluAsp: 4.622 ± 0.569
3.302GluGlu: 3.302 ± 0.856
2.495GluPhe: 2.495 ± 0.364
3.375GluGly: 3.375 ± 0.513
1.174GluHis: 1.174 ± 0.275
4.916GluIle: 4.916 ± 0.595
4.329GluLys: 4.329 ± 0.568
5.943GluLeu: 5.943 ± 0.623
1.908GluMet: 1.908 ± 0.368
2.495GluAsn: 2.495 ± 0.399
1.908GluPro: 1.908 ± 0.376
3.008GluGln: 3.008 ± 0.555
3.522GluArg: 3.522 ± 0.49
4.182GluSer: 4.182 ± 0.683
3.449GluThr: 3.449 ± 0.461
3.889GluVal: 3.889 ± 0.472
0.66GluTrp: 0.66 ± 0.258
1.541GluTyr: 1.541 ± 0.344
0.0GluXaa: 0.0 ± 0.0
Phe
2.788PheAla: 2.788 ± 0.471
0.367PheCys: 0.367 ± 0.159
3.595PheAsp: 3.595 ± 0.496
2.421PheGlu: 2.421 ± 0.372
1.321PhePhe: 1.321 ± 0.417
4.549PheGly: 4.549 ± 0.517
0.293PheHis: 0.293 ± 0.2
2.495PheIle: 2.495 ± 0.423
2.421PheLys: 2.421 ± 0.452
2.862PheLeu: 2.862 ± 0.388
0.954PheMet: 0.954 ± 0.255
3.302PheAsn: 3.302 ± 0.462
0.734PhePro: 0.734 ± 0.188
0.587PheGln: 0.587 ± 0.206
1.761PheArg: 1.761 ± 0.379
2.935PheSer: 2.935 ± 0.538
1.541PheThr: 1.541 ± 0.328
2.348PheVal: 2.348 ± 0.504
0.807PheTrp: 0.807 ± 0.285
1.247PheTyr: 1.247 ± 0.308
0.0PheXaa: 0.0 ± 0.0
Gly
5.65GlyAla: 5.65 ± 0.66
0.734GlyCys: 0.734 ± 0.273
3.962GlyAsp: 3.962 ± 0.802
6.09GlyGlu: 6.09 ± 0.717
4.109GlyPhe: 4.109 ± 0.598
5.87GlyGly: 5.87 ± 0.88
1.467GlyHis: 1.467 ± 0.271
4.329GlyIle: 4.329 ± 0.578
4.256GlyLys: 4.256 ± 0.62
5.136GlyLeu: 5.136 ± 0.623
1.321GlyMet: 1.321 ± 0.255
3.228GlyAsn: 3.228 ± 0.426
1.027GlyPro: 1.027 ± 0.266
2.201GlyGln: 2.201 ± 0.49
3.155GlyArg: 3.155 ± 0.432
4.549GlySer: 4.549 ± 0.543
4.109GlyThr: 4.109 ± 0.598
6.163GlyVal: 6.163 ± 0.651
1.027GlyTrp: 1.027 ± 0.267
2.862GlyTyr: 2.862 ± 0.413
0.0GlyXaa: 0.0 ± 0.0
His
1.467HisAla: 1.467 ± 0.345
0.22HisCys: 0.22 ± 0.12
1.467HisAsp: 1.467 ± 0.32
0.88HisGlu: 0.88 ± 0.239
0.514HisPhe: 0.514 ± 0.177
0.954HisGly: 0.954 ± 0.303
0.22HisHis: 0.22 ± 0.098
1.027HisIle: 1.027 ± 0.314
1.321HisLys: 1.321 ± 0.288
1.101HisLeu: 1.101 ± 0.261
0.147HisMet: 0.147 ± 0.093
0.367HisAsn: 0.367 ± 0.162
0.587HisPro: 0.587 ± 0.191
0.807HisGln: 0.807 ± 0.233
0.587HisArg: 0.587 ± 0.195
1.101HisSer: 1.101 ± 0.229
0.807HisThr: 0.807 ± 0.214
0.954HisVal: 0.954 ± 0.3
0.147HisTrp: 0.147 ± 0.091
0.66HisTyr: 0.66 ± 0.264
0.0HisXaa: 0.0 ± 0.0
Ile
6.383IleAla: 6.383 ± 0.637
0.367IleCys: 0.367 ± 0.164
4.916IleAsp: 4.916 ± 0.668
4.696IleGlu: 4.696 ± 0.53
1.834IlePhe: 1.834 ± 0.417
4.769IleGly: 4.769 ± 0.65
0.734IleHis: 0.734 ± 0.238
4.476IleIle: 4.476 ± 0.612
5.209IleLys: 5.209 ± 0.597
4.036IleLeu: 4.036 ± 0.528
2.128IleMet: 2.128 ± 0.365
4.769IleAsn: 4.769 ± 0.505
2.275IlePro: 2.275 ± 0.406
2.275IleGln: 2.275 ± 0.334
2.275IleArg: 2.275 ± 0.401
5.356IleSer: 5.356 ± 0.683
4.549IleThr: 4.549 ± 0.581
4.329IleVal: 4.329 ± 0.615
0.44IleTrp: 0.44 ± 0.166
2.495IleTyr: 2.495 ± 0.529
0.0IleXaa: 0.0 ± 0.0
Lys
6.604LysAla: 6.604 ± 0.785
1.101LysCys: 1.101 ± 0.345
4.036LysAsp: 4.036 ± 0.784
3.815LysGlu: 3.815 ± 0.58
2.275LysPhe: 2.275 ± 0.436
3.228LysGly: 3.228 ± 0.492
1.614LysHis: 1.614 ± 0.455
4.989LysIle: 4.989 ± 0.587
4.622LysLys: 4.622 ± 0.77
5.356LysLeu: 5.356 ± 0.641
2.568LysMet: 2.568 ± 0.625
3.155LysAsn: 3.155 ± 0.427
2.862LysPro: 2.862 ± 0.493
2.641LysGln: 2.641 ± 0.463
3.742LysArg: 3.742 ± 0.478
4.989LysSer: 4.989 ± 0.668
4.036LysThr: 4.036 ± 0.606
4.036LysVal: 4.036 ± 0.558
1.321LysTrp: 1.321 ± 0.311
3.155LysTyr: 3.155 ± 0.511
0.0LysXaa: 0.0 ± 0.0
Leu
7.191LeuAla: 7.191 ± 0.883
0.88LeuCys: 0.88 ± 0.229
5.576LeuAsp: 5.576 ± 0.783
4.476LeuGlu: 4.476 ± 0.609
2.348LeuPhe: 2.348 ± 0.405
4.769LeuGly: 4.769 ± 0.629
0.66LeuHis: 0.66 ± 0.194
4.036LeuIle: 4.036 ± 0.578
5.209LeuLys: 5.209 ± 0.614
5.503LeuLeu: 5.503 ± 0.708
1.834LeuMet: 1.834 ± 0.398
3.815LeuAsn: 3.815 ± 0.502
3.155LeuPro: 3.155 ± 0.448
2.715LeuGln: 2.715 ± 0.401
3.082LeuArg: 3.082 ± 0.448
6.237LeuSer: 6.237 ± 0.641
4.256LeuThr: 4.256 ± 0.536
4.476LeuVal: 4.476 ± 0.574
0.734LeuTrp: 0.734 ± 0.227
1.908LeuTyr: 1.908 ± 0.381
0.0LeuXaa: 0.0 ± 0.0
Met
3.375MetAla: 3.375 ± 0.469
0.073MetCys: 0.073 ± 0.078
1.467MetAsp: 1.467 ± 0.447
1.174MetGlu: 1.174 ± 0.28
0.88MetPhe: 0.88 ± 0.241
1.541MetGly: 1.541 ± 0.39
0.293MetHis: 0.293 ± 0.125
2.348MetIle: 2.348 ± 0.432
2.715MetLys: 2.715 ± 0.421
2.054MetLeu: 2.054 ± 0.412
0.807MetMet: 0.807 ± 0.215
1.321MetAsn: 1.321 ± 0.33
0.954MetPro: 0.954 ± 0.323
0.807MetGln: 0.807 ± 0.222
1.467MetArg: 1.467 ± 0.33
3.008MetSer: 3.008 ± 0.577
2.201MetThr: 2.201 ± 0.497
0.807MetVal: 0.807 ± 0.26
0.367MetTrp: 0.367 ± 0.183
0.367MetTyr: 0.367 ± 0.173
0.0MetXaa: 0.0 ± 0.0
Asn
3.742AsnAla: 3.742 ± 0.545
0.514AsnCys: 0.514 ± 0.181
2.935AsnAsp: 2.935 ± 0.412
2.641AsnGlu: 2.641 ± 0.346
2.641AsnPhe: 2.641 ± 0.497
6.017AsnGly: 6.017 ± 0.687
0.807AsnHis: 0.807 ± 0.223
3.302AsnIle: 3.302 ± 0.465
3.302AsnLys: 3.302 ± 0.572
3.008AsnLeu: 3.008 ± 0.447
1.247AsnMet: 1.247 ± 0.263
2.348AsnAsn: 2.348 ± 0.436
2.641AsnPro: 2.641 ± 0.419
1.834AsnGln: 1.834 ± 0.462
2.128AsnArg: 2.128 ± 0.334
3.155AsnSer: 3.155 ± 0.475
3.375AsnThr: 3.375 ± 0.464
4.109AsnVal: 4.109 ± 0.512
0.44AsnTrp: 0.44 ± 0.165
2.128AsnTyr: 2.128 ± 0.376
0.0AsnXaa: 0.0 ± 0.0
Pro
3.449ProAla: 3.449 ± 0.602
0.293ProCys: 0.293 ± 0.137
2.201ProAsp: 2.201 ± 0.53
2.495ProGlu: 2.495 ± 0.444
1.541ProPhe: 1.541 ± 0.309
1.174ProGly: 1.174 ± 0.328
0.44ProHis: 0.44 ± 0.171
2.201ProIle: 2.201 ± 0.449
2.128ProLys: 2.128 ± 0.37
2.275ProLeu: 2.275 ± 0.368
0.954ProMet: 0.954 ± 0.231
1.981ProAsn: 1.981 ± 0.372
1.467ProPro: 1.467 ± 0.367
1.174ProGln: 1.174 ± 0.246
1.027ProArg: 1.027 ± 0.242
2.421ProSer: 2.421 ± 0.306
2.128ProThr: 2.128 ± 0.51
3.375ProVal: 3.375 ± 0.597
0.44ProTrp: 0.44 ± 0.166
0.587ProTyr: 0.587 ± 0.189
0.0ProXaa: 0.0 ± 0.0
Gln
3.228GlnAla: 3.228 ± 0.637
0.587GlnCys: 0.587 ± 0.272
1.688GlnAsp: 1.688 ± 0.413
2.054GlnGlu: 2.054 ± 0.388
1.027GlnPhe: 1.027 ± 0.295
1.981GlnGly: 1.981 ± 0.483
0.734GlnHis: 0.734 ± 0.2
1.688GlnIle: 1.688 ± 0.354
2.275GlnLys: 2.275 ± 0.369
2.862GlnLeu: 2.862 ± 0.515
0.66GlnMet: 0.66 ± 0.244
2.128GlnAsn: 2.128 ± 0.348
0.587GlnPro: 0.587 ± 0.202
1.908GlnGln: 1.908 ± 0.556
1.981GlnArg: 1.981 ± 0.416
2.568GlnSer: 2.568 ± 0.417
1.908GlnThr: 1.908 ± 0.311
2.715GlnVal: 2.715 ± 0.412
0.66GlnTrp: 0.66 ± 0.209
1.174GlnTyr: 1.174 ± 0.269
0.0GlnXaa: 0.0 ± 0.0
Arg
3.228ArgAla: 3.228 ± 0.485
0.44ArgCys: 0.44 ± 0.19
2.788ArgAsp: 2.788 ± 0.557
2.348ArgGlu: 2.348 ± 0.521
2.054ArgPhe: 2.054 ± 0.406
2.348ArgGly: 2.348 ± 0.361
0.66ArgHis: 0.66 ± 0.234
3.228ArgIle: 3.228 ± 0.582
2.275ArgLys: 2.275 ± 0.426
3.449ArgLeu: 3.449 ± 0.541
1.688ArgMet: 1.688 ± 0.286
2.201ArgAsn: 2.201 ± 0.322
1.321ArgPro: 1.321 ± 0.288
1.834ArgGln: 1.834 ± 0.396
1.761ArgArg: 1.761 ± 0.431
2.935ArgSer: 2.935 ± 0.416
1.614ArgThr: 1.614 ± 0.295
3.082ArgVal: 3.082 ± 0.435
0.44ArgTrp: 0.44 ± 0.19
2.201ArgTyr: 2.201 ± 0.311
0.0ArgXaa: 0.0 ± 0.0
Ser
6.677SerAla: 6.677 ± 0.732
0.44SerCys: 0.44 ± 0.163
4.256SerAsp: 4.256 ± 0.659
3.889SerGlu: 3.889 ± 0.653
3.522SerPhe: 3.522 ± 0.51
5.503SerGly: 5.503 ± 0.637
0.88SerHis: 0.88 ± 0.202
4.989SerIle: 4.989 ± 0.531
6.163SerLys: 6.163 ± 0.746
5.65SerLeu: 5.65 ± 0.749
1.981SerMet: 1.981 ± 0.399
3.008SerAsn: 3.008 ± 0.479
2.715SerPro: 2.715 ± 0.381
1.834SerGln: 1.834 ± 0.422
3.008SerArg: 3.008 ± 0.436
5.209SerSer: 5.209 ± 0.639
4.696SerThr: 4.696 ± 0.713
4.916SerVal: 4.916 ± 0.584
0.367SerTrp: 0.367 ± 0.143
2.495SerTyr: 2.495 ± 0.514
0.0SerXaa: 0.0 ± 0.0
Thr
4.402ThrAla: 4.402 ± 0.574
0.807ThrCys: 0.807 ± 0.267
3.889ThrAsp: 3.889 ± 0.684
3.889ThrGlu: 3.889 ± 0.574
1.761ThrPhe: 1.761 ± 0.415
5.576ThrGly: 5.576 ± 0.697
0.66ThrHis: 0.66 ± 0.228
3.522ThrIle: 3.522 ± 0.529
4.622ThrLys: 4.622 ± 0.651
4.696ThrLeu: 4.696 ± 0.568
1.541ThrMet: 1.541 ± 0.332
2.495ThrAsn: 2.495 ± 0.415
3.375ThrPro: 3.375 ± 0.46
2.054ThrGln: 2.054 ± 0.459
1.761ThrArg: 1.761 ± 0.396
3.889ThrSer: 3.889 ± 0.526
3.742ThrThr: 3.742 ± 0.66
3.669ThrVal: 3.669 ± 0.727
0.954ThrTrp: 0.954 ± 0.25
1.541ThrTyr: 1.541 ± 0.325
0.0ThrXaa: 0.0 ± 0.0
Val
5.796ValAla: 5.796 ± 0.583
0.807ValCys: 0.807 ± 0.279
4.329ValAsp: 4.329 ± 0.376
4.622ValGlu: 4.622 ± 0.516
3.375ValPhe: 3.375 ± 0.528
4.622ValGly: 4.622 ± 0.806
1.027ValHis: 1.027 ± 0.286
4.769ValIle: 4.769 ± 0.54
5.283ValLys: 5.283 ± 0.598
2.568ValLeu: 2.568 ± 0.431
1.834ValMet: 1.834 ± 0.426
4.622ValAsn: 4.622 ± 0.574
2.275ValPro: 2.275 ± 0.402
2.275ValGln: 2.275 ± 0.393
3.082ValArg: 3.082 ± 0.517
5.209ValSer: 5.209 ± 0.494
4.916ValThr: 4.916 ± 0.774
3.742ValVal: 3.742 ± 0.581
1.247ValTrp: 1.247 ± 0.31
1.394ValTyr: 1.394 ± 0.291
0.0ValXaa: 0.0 ± 0.0
Trp
0.66TrpAla: 0.66 ± 0.2
0.22TrpCys: 0.22 ± 0.131
0.587TrpAsp: 0.587 ± 0.197
0.587TrpGlu: 0.587 ± 0.15
0.367TrpPhe: 0.367 ± 0.16
1.101TrpGly: 1.101 ± 0.293
0.44TrpHis: 0.44 ± 0.156
0.66TrpIle: 0.66 ± 0.219
0.587TrpLys: 0.587 ± 0.194
1.321TrpLeu: 1.321 ± 0.343
0.514TrpMet: 0.514 ± 0.171
0.514TrpAsn: 0.514 ± 0.202
0.367TrpPro: 0.367 ± 0.142
0.734TrpGln: 0.734 ± 0.254
0.514TrpArg: 0.514 ± 0.178
1.027TrpSer: 1.027 ± 0.234
0.514TrpThr: 0.514 ± 0.201
1.467TrpVal: 1.467 ± 0.348
0.147TrpTrp: 0.147 ± 0.127
0.514TrpTyr: 0.514 ± 0.18
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.155TyrAla: 3.155 ± 0.598
0.44TyrCys: 0.44 ± 0.184
2.568TyrAsp: 2.568 ± 0.48
1.834TyrGlu: 1.834 ± 0.335
1.394TyrPhe: 1.394 ± 0.368
1.321TyrGly: 1.321 ± 0.288
0.44TyrHis: 0.44 ± 0.192
2.568TyrIle: 2.568 ± 0.389
1.908TyrLys: 1.908 ± 0.37
2.568TyrLeu: 2.568 ± 0.441
0.954TyrMet: 0.954 ± 0.274
2.495TyrAsn: 2.495 ± 0.419
0.954TyrPro: 0.954 ± 0.266
0.954TyrGln: 0.954 ± 0.249
1.321TyrArg: 1.321 ± 0.287
2.568TyrSer: 2.568 ± 0.402
2.054TyrThr: 2.054 ± 0.367
1.908TyrVal: 1.908 ± 0.321
0.514TyrTrp: 0.514 ± 0.229
1.321TyrTyr: 1.321 ± 0.308
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 69 proteins (13630 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski