Escherichia phage C1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G3M7A5|A0A3G3M7A5_9CAUD Putative head assembly protein OS=Escherichia phage C1 OX=2340716 PE=4 SV=1
MM1 pKa = 7.65SEE3 pKa = 3.94VKK5 pKa = 10.31RR6 pKa = 11.84YY7 pKa = 10.05DD8 pKa = 2.94ISAFGSMSMDD18 pKa = 3.07EE19 pKa = 5.08CGDD22 pKa = 3.55GAYY25 pKa = 10.85VNYY28 pKa = 10.1EE29 pKa = 4.02DD30 pKa = 4.96YY31 pKa = 11.68AEE33 pKa = 5.29LEE35 pKa = 4.34AKK37 pKa = 10.09CAEE40 pKa = 4.24LAAEE44 pKa = 4.06NAALKK49 pKa = 10.32QSEE52 pKa = 4.5KK53 pKa = 10.75EE54 pKa = 3.89FNNFCRR60 pKa = 11.84QEE62 pKa = 4.09YY63 pKa = 10.16YY64 pKa = 10.8GWEE67 pKa = 4.35DD68 pKa = 3.69NLTEE72 pKa = 4.43TPATDD77 pKa = 3.74DD78 pKa = 3.41FLAEE82 pKa = 3.82VRR84 pKa = 11.84AQGVEE89 pKa = 3.69MAMEE93 pKa = 5.03HH94 pKa = 5.64MQSSGSLTFGDD105 pKa = 5.23CYY107 pKa = 10.87ISLNEE112 pKa = 3.86FAAEE116 pKa = 3.86LRR118 pKa = 11.84KK119 pKa = 10.62GGWQKK124 pKa = 11.52

Molecular weight: 13.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G3M8E9|A0A3G3M8E9_9CAUD Uncharacterized protein OS=Escherichia phage C1 OX=2340716 PE=4 SV=1
MM1 pKa = 7.8AKK3 pKa = 9.86TIYY6 pKa = 9.96RR7 pKa = 11.84RR8 pKa = 11.84EE9 pKa = 4.01KK10 pKa = 10.21LEE12 pKa = 3.76QEE14 pKa = 4.24LGHH17 pKa = 7.05VGAQNFMSKK26 pKa = 7.49QARR29 pKa = 11.84NAMEE33 pKa = 4.66SIRR36 pKa = 11.84VNRR39 pKa = 11.84VVRR42 pKa = 11.84VFNGEE47 pKa = 3.69GKK49 pKa = 10.08RR50 pKa = 11.84RR51 pKa = 11.84VMDD54 pKa = 3.54EE55 pKa = 4.36LIIVFF60 pKa = 4.5

Molecular weight: 7.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

13600

31

970

178.9

20.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.096 ± 0.703

1.39 ± 0.202

6.118 ± 0.284

6.441 ± 0.321

3.603 ± 0.201

6.934 ± 0.278

1.625 ± 0.184

6.566 ± 0.233

6.537 ± 0.357

7.294 ± 0.213

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.272 ± 0.2

4.757 ± 0.25

3.316 ± 0.238

4.0 ± 0.412

5.088 ± 0.285

6.353 ± 0.423

5.676 ± 0.396

6.419 ± 0.241

1.654 ± 0.171

3.86 ± 0.207
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski