Streptococcus phage Javan126

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AQI0|A0A4D6AQI0_9CAUD HTH cro/C1-type domain-containing protein OS=Streptococcus phage Javan126 OX=2547992 GN=Javan126_0008 PE=4 SV=1
MM1 pKa = 7.26ATLDD5 pKa = 3.84EE6 pKa = 4.51VLSFAKK12 pKa = 10.53GLADD16 pKa = 3.48TGQGVDD22 pKa = 3.78LDD24 pKa = 4.08NVYY27 pKa = 8.53GTQCVDD33 pKa = 3.5LPNWITTKK41 pKa = 10.75YY42 pKa = 10.5FGIALWGNAIDD53 pKa = 5.11LLDD56 pKa = 3.86SAAAQGMEE64 pKa = 4.21VVYY67 pKa = 10.3NAPGVNPRR75 pKa = 11.84AGAIFVMVTYY85 pKa = 10.75AHH87 pKa = 7.21GYY89 pKa = 7.86GHH91 pKa = 7.1TGLVIVTSDD100 pKa = 3.56GYY102 pKa = 10.61VLHH105 pKa = 7.03NIEE108 pKa = 4.75QNVDD112 pKa = 3.04GNADD116 pKa = 3.33ALYY119 pKa = 9.95IGGPARR125 pKa = 11.84YY126 pKa = 9.17VDD128 pKa = 4.39RR129 pKa = 11.84PFEE132 pKa = 5.2DD133 pKa = 3.37GTGYY137 pKa = 10.44ILGWFYY143 pKa = 10.79PPYY146 pKa = 10.6DD147 pKa = 4.01STPAAATEE155 pKa = 3.99PSAPVVAQSDD165 pKa = 3.67GTYY168 pKa = 10.39VVNPEE173 pKa = 3.95TGTFTVRR180 pKa = 11.84VAALNVRR187 pKa = 11.84SAPRR191 pKa = 11.84LDD193 pKa = 3.6AEE195 pKa = 4.28IVATYY200 pKa = 11.02GEE202 pKa = 4.32NTEE205 pKa = 4.2FNYY208 pKa = 10.93DD209 pKa = 2.91GWIDD213 pKa = 3.51SDD215 pKa = 4.15GYY217 pKa = 10.69IWVTYY222 pKa = 10.35ISVTGVRR229 pKa = 11.84RR230 pKa = 11.84YY231 pKa = 9.74VAVGNSQNGRR241 pKa = 11.84RR242 pKa = 11.84VTNFGTFRR250 pKa = 4.37

Molecular weight: 26.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6ATI1|A0A4D6ATI1_9CAUD Uncharacterized protein OS=Streptococcus phage Javan126 OX=2547992 GN=Javan126_0026 PE=4 SV=1
MM1 pKa = 6.82TTDD4 pKa = 4.11EE5 pKa = 4.69LLQNLRR11 pKa = 11.84DD12 pKa = 4.16DD13 pKa = 4.59LNKK16 pKa = 10.2IMNVLKK22 pKa = 10.73NDD24 pKa = 3.12WKK26 pKa = 11.23ALLFLAIAIFGMMVTVSYY44 pKa = 10.2FSYY47 pKa = 10.7RR48 pKa = 11.84DD49 pKa = 3.12ARR51 pKa = 11.84QYY53 pKa = 11.17YY54 pKa = 8.38EE55 pKa = 3.72PQIYY59 pKa = 9.99GLRR62 pKa = 11.84AQLSRR67 pKa = 11.84TQKK70 pKa = 8.3QLKK73 pKa = 9.28RR74 pKa = 11.84ASEE77 pKa = 3.86QNQRR81 pKa = 11.84QTKK84 pKa = 10.05RR85 pKa = 11.84IAEE88 pKa = 4.08LTGNGGG94 pKa = 3.26

Molecular weight: 10.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

10807

38

1366

203.9

23.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.635 ± 0.723

0.426 ± 0.079

6.773 ± 0.325

7.884 ± 0.412

3.923 ± 0.245

5.968 ± 0.353

1.286 ± 0.154

6.949 ± 0.269

9.253 ± 0.38

8.698 ± 0.343

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.489 ± 0.195

5.922 ± 0.287

2.887 ± 0.195

3.97 ± 0.227

4.312 ± 0.255

5.904 ± 0.296

5.774 ± 0.284

6.079 ± 0.294

1.129 ± 0.08

3.738 ± 0.37
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski