Litorilinea aerophila

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Chloroflexi; Caldilineae; Caldilineales; Caldilineaceae; Litorilinea

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4877 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A540VGA9|A0A540VGA9_9CHLR SpoVR family protein OS=Litorilinea aerophila OX=1204385 GN=FKZ61_13430 PE=4 SV=1
MM1 pKa = 7.46TKK3 pKa = 9.95PNRR6 pKa = 11.84FIAPVCAWAFGLGLLMALVTGLTVTLSPQRR36 pKa = 11.84VTAAPLPVPHH46 pKa = 6.68FVPTLLSTTPDD57 pKa = 2.85ANARR61 pKa = 11.84HH62 pKa = 5.66VPRR65 pKa = 11.84NTAVSASFQEE75 pKa = 4.03AMNPSSVTPSTFVVHH90 pKa = 6.85GSQSGLIAGTYY101 pKa = 9.27AVSGDD106 pKa = 3.69GQTVSLTPAASLFPGEE122 pKa = 4.45RR123 pKa = 11.84VDD125 pKa = 5.97AILTTGLANTSGEE138 pKa = 4.23PLDD141 pKa = 5.38APHH144 pKa = 6.73VWQFWAQAGIATGIYY159 pKa = 9.73GFSGQPLGAANQQSYY174 pKa = 10.43AVAAGDD180 pKa = 4.01LNQDD184 pKa = 3.86GFPDD188 pKa = 4.09LVVGNAKK195 pKa = 10.52AGGHH199 pKa = 4.4NTIYY203 pKa = 10.91LNDD206 pKa = 3.81GTGSFGTISQTLPTLDD222 pKa = 3.32STRR225 pKa = 11.84SVLLVDD231 pKa = 3.95MDD233 pKa = 3.81MDD235 pKa = 4.77GDD237 pKa = 4.19LDD239 pKa = 4.25LVVGNHH245 pKa = 5.85GKK247 pKa = 9.94QNYY250 pKa = 9.52IYY252 pKa = 10.22LNNGNGTFATPGLPFGPDD270 pKa = 2.84NDD272 pKa = 4.15TTYY275 pKa = 11.67SLAAGDD281 pKa = 4.25LDD283 pKa = 4.5GNGTIDD289 pKa = 3.72LVVGNVITSPMGIYY303 pKa = 10.27FNNGNATFSSQSYY316 pKa = 7.11TLPVEE321 pKa = 4.22GQVWSVALGDD331 pKa = 3.65VDD333 pKa = 5.22GDD335 pKa = 3.84GDD337 pKa = 4.63LDD339 pKa = 4.09LAVGNYY345 pKa = 9.91SNPNQIFLNDD355 pKa = 3.3GHH357 pKa = 7.3GNFIQPGINLRR368 pKa = 11.84DD369 pKa = 3.83ATDD372 pKa = 3.48LTHH375 pKa = 7.28RR376 pKa = 11.84LVLGDD381 pKa = 3.46VDD383 pKa = 5.04GDD385 pKa = 3.87GDD387 pKa = 4.59LDD389 pKa = 4.13AVTANAGLNYY399 pKa = 10.03VYY401 pKa = 10.85YY402 pKa = 10.84NNGDD406 pKa = 3.58GTFSANRR413 pKa = 11.84HH414 pKa = 5.4PFGVSYY420 pKa = 10.68DD421 pKa = 3.6NSMGLALGDD430 pKa = 3.49VDD432 pKa = 5.64GDD434 pKa = 3.67GDD436 pKa = 4.75LDD438 pKa = 3.76VVVANDD444 pKa = 3.02VGAAKK449 pKa = 10.73NPGEE453 pKa = 4.38SGNVVYY459 pKa = 10.98LNDD462 pKa = 3.76GDD464 pKa = 4.33GTFDD468 pKa = 3.06TTAYY472 pKa = 10.74LFGGVSDD479 pKa = 4.54RR480 pKa = 11.84SQAVALADD488 pKa = 3.81FDD490 pKa = 5.26GDD492 pKa = 3.92GDD494 pKa = 4.56LDD496 pKa = 3.97LAAVNAAMAGEE507 pKa = 4.22NPVPQQSAVYY517 pKa = 8.94FNANASDD524 pKa = 3.81PAITFSMQPTSTVPGGTVRR543 pKa = 11.84YY544 pKa = 8.64FLRR547 pKa = 11.84FQNQGQAASHH557 pKa = 4.99QVIITITLPAQVTITNIEE575 pKa = 4.01TSGAPVTMSGSGQIRR590 pKa = 11.84VFQAGSLAAQASGQITVTGTLVTGLAPNTQFSATASITGSGDD632 pKa = 3.43GYY634 pKa = 11.17LLNNNASAVGQVLNGPPNAVDD655 pKa = 4.3DD656 pKa = 4.47SFTIPEE662 pKa = 4.64DD663 pKa = 3.78SPNTALSVLANDD675 pKa = 4.33SDD677 pKa = 4.65PNGDD681 pKa = 3.92PLSIYY686 pKa = 10.77AVGIPDD692 pKa = 3.43HH693 pKa = 6.6GGVVNFNASTITYY706 pKa = 9.75RR707 pKa = 11.84PAANFAGTEE716 pKa = 4.13VFTYY720 pKa = 7.43TARR723 pKa = 11.84DD724 pKa = 3.38GRR726 pKa = 11.84GGYY729 pKa = 7.9GTANVIVTVTPVNDD743 pKa = 3.88PPDD746 pKa = 3.71ARR748 pKa = 11.84NDD750 pKa = 3.55SATVDD755 pKa = 3.8EE756 pKa = 5.27DD757 pKa = 4.31SGDD760 pKa = 3.55TFIDD764 pKa = 3.35VLANDD769 pKa = 4.41TSAPDD774 pKa = 3.27TGEE777 pKa = 4.06TLTVIAVGTPSRR789 pKa = 11.84GGTVSFTPGGVTYY802 pKa = 10.56RR803 pKa = 11.84PAPNYY808 pKa = 10.61NGVEE812 pKa = 4.22TFTYY816 pKa = 9.75TISDD820 pKa = 3.94GNGGTDD826 pKa = 3.21SAQVTVTVNPVNDD839 pKa = 4.11PPDD842 pKa = 3.82ARR844 pKa = 11.84DD845 pKa = 3.43DD846 pKa = 3.83TATVDD851 pKa = 5.14EE852 pKa = 5.25DD853 pKa = 4.25SQDD856 pKa = 3.33NLINVLQNDD865 pKa = 5.2RR866 pKa = 11.84ITPDD870 pKa = 2.71VDD872 pKa = 4.14EE873 pKa = 5.11ILTVIAVGTPSQGGTATLTGGQVRR897 pKa = 11.84YY898 pKa = 7.93TPAPNFFGIEE908 pKa = 4.14TFTYY912 pKa = 9.73TISDD916 pKa = 3.94GNGGTDD922 pKa = 3.21SAQVTVTVNPVNDD935 pKa = 4.11PPDD938 pKa = 3.82ARR940 pKa = 11.84DD941 pKa = 3.43DD942 pKa = 3.83TATVDD947 pKa = 4.36EE948 pKa = 5.21DD949 pKa = 3.97SQNNLINVLANDD961 pKa = 4.19TSAPDD966 pKa = 3.27TGEE969 pKa = 4.13TLTVTAVGTPSQGGTATLTGGQVRR993 pKa = 11.84YY994 pKa = 7.93TPAPNFFGIEE1004 pKa = 4.14TFTYY1008 pKa = 9.73TISDD1012 pKa = 3.94GNGGTDD1018 pKa = 3.2SAQVSVTVNPVNDD1031 pKa = 4.11PPDD1034 pKa = 3.82ARR1036 pKa = 11.84DD1037 pKa = 3.46DD1038 pKa = 3.94TATVLQNAGATPINVLANDD1057 pKa = 4.51TYY1059 pKa = 11.53LPDD1062 pKa = 4.05APEE1065 pKa = 3.93TLTIVGVGVASAGGSVSFSVDD1086 pKa = 3.05QVFYY1090 pKa = 10.13TPPPNFIGTDD1100 pKa = 3.2TFAYY1104 pKa = 8.68TISDD1108 pKa = 3.87GNGGTDD1114 pKa = 3.21SAQVTVTVTPSSNPPANHH1132 pKa = 6.76PPAARR1137 pKa = 11.84DD1138 pKa = 3.6DD1139 pKa = 3.92VATVSKK1145 pKa = 10.58NSHH1148 pKa = 5.38SNRR1151 pKa = 11.84LDD1153 pKa = 3.22VLANDD1158 pKa = 3.65STEE1161 pKa = 4.2PDD1163 pKa = 3.05TGEE1166 pKa = 3.85QLSIIAVNDD1175 pKa = 3.77LSAGGTVTFTARR1187 pKa = 11.84YY1188 pKa = 9.3VFYY1191 pKa = 10.3TPKK1194 pKa = 10.12TDD1196 pKa = 3.75FTGVEE1201 pKa = 4.18TFAYY1205 pKa = 9.19TVDD1208 pKa = 3.87DD1209 pKa = 4.35GRR1211 pKa = 11.84GGTDD1215 pKa = 2.8WAVVTVTVTGTTGSPGNPPGDD1236 pKa = 4.41PSPGDD1241 pKa = 3.42PPGTGNRR1248 pKa = 11.84APVAVNDD1255 pKa = 4.06NYY1257 pKa = 11.04STVQGQTLTVSAAEE1271 pKa = 4.17GVLQNDD1277 pKa = 3.2QDD1279 pKa = 3.93EE1280 pKa = 5.17DD1281 pKa = 4.75GDD1283 pKa = 4.13TLTAEE1288 pKa = 4.46LVADD1292 pKa = 4.4VVQGTLSLQPDD1303 pKa = 4.04GSFVYY1308 pKa = 8.74TAPATFSGTTTFSYY1322 pKa = 10.37RR1323 pKa = 11.84AYY1325 pKa = 10.58DD1326 pKa = 3.52GQAYY1330 pKa = 10.11SGAATVVISVQAEE1343 pKa = 4.0SSGNLEE1349 pKa = 3.75LDD1351 pKa = 3.39KK1352 pKa = 11.27QIFLPVIQRR1361 pKa = 11.84ATEE1364 pKa = 3.7

Molecular weight: 140.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A540V944|A0A540V944_9CHLR N-acetylmuramic acid 6-phosphate etherase OS=Litorilinea aerophila OX=1204385 GN=murQ PE=3 SV=1
MM1 pKa = 8.05PKK3 pKa = 9.16RR4 pKa = 11.84TWQPKK9 pKa = 6.52VRR11 pKa = 11.84KK12 pKa = 9.2RR13 pKa = 11.84LKK15 pKa = 8.26THH17 pKa = 5.86GFRR20 pKa = 11.84IRR22 pKa = 11.84MRR24 pKa = 11.84TPGGRR29 pKa = 11.84NVLKK33 pKa = 10.49RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84LKK38 pKa = 10.44GRR40 pKa = 11.84ARR42 pKa = 11.84LTVKK46 pKa = 9.51LTEE49 pKa = 4.24RR50 pKa = 11.84KK51 pKa = 9.26PFRR54 pKa = 11.84HH55 pKa = 6.02

Molecular weight: 6.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4877

0

4877

1775820

26

6596

364.1

40.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.031 ± 0.046

0.824 ± 0.011

5.044 ± 0.028

6.06 ± 0.038

3.528 ± 0.021

8.153 ± 0.028

2.309 ± 0.019

4.818 ± 0.031

2.051 ± 0.025

11.286 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.117 ± 0.017

2.591 ± 0.022

6.215 ± 0.031

4.454 ± 0.02

7.172 ± 0.039

4.623 ± 0.024

5.321 ± 0.043

7.67 ± 0.034

1.844 ± 0.019

2.888 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski