Bacteroides plebeius CAG:211

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; environmental samples

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2643 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5VRJ4|R5VRJ4_9BACE Uncharacterized protein OS=Bacteroides plebeius CAG:211 OX=1263052 GN=BN536_01077 PE=4 SV=1
MM1 pKa = 7.16KK2 pKa = 9.74TIKK5 pKa = 10.6SLFAVLFALLAFSACEE21 pKa = 3.72KK22 pKa = 10.93DD23 pKa = 3.14GDD25 pKa = 4.36KK26 pKa = 10.55IYY28 pKa = 10.82LQSLEE33 pKa = 4.71SNNLMATTDD42 pKa = 3.66RR43 pKa = 11.84VEE45 pKa = 4.44LNADD49 pKa = 4.28LAQEE53 pKa = 4.27IVVSLAWTDD62 pKa = 3.36RR63 pKa = 11.84TIQISDD69 pKa = 3.85PAVGTTVTVTNYY81 pKa = 9.91VEE83 pKa = 4.93ASLSEE88 pKa = 4.37DD89 pKa = 3.28FSTTVSKK96 pKa = 9.2TATTSLSIAFTGLEE110 pKa = 4.2LNSLASEE117 pKa = 4.23IGAVTGRR124 pKa = 11.84NNKK127 pKa = 9.58IYY129 pKa = 10.36FRR131 pKa = 11.84LAGTTGTNIPLVYY144 pKa = 10.42SNVVSIDD151 pKa = 3.37VTPYY155 pKa = 11.08KK156 pKa = 10.07MDD158 pKa = 3.62MNTGTVILSNDD169 pKa = 2.97QTEE172 pKa = 4.4NLGEE176 pKa = 4.22TGVTFYY182 pKa = 11.41SPDD185 pKa = 3.27ADD187 pKa = 4.09GIYY190 pKa = 10.35SGFISIPEE198 pKa = 3.73AWYY201 pKa = 9.4HH202 pKa = 5.88ILLKK206 pKa = 10.86EE207 pKa = 4.12GDD209 pKa = 3.55GSIWGTSGSEE219 pKa = 4.31GAFHH223 pKa = 7.23LTDD226 pKa = 4.63DD227 pKa = 5.07PNNSLQNMWFPGTAGCYY244 pKa = 8.57YY245 pKa = 10.78VIVNTQAAEE254 pKa = 3.77WSALHH259 pKa = 6.56ISSLLVSGLTDD270 pKa = 3.26EE271 pKa = 5.59SISLTLDD278 pKa = 2.88QTNNQWLATFNAEE291 pKa = 4.18TAGSRR296 pKa = 11.84TITINGKK303 pKa = 9.15GEE305 pKa = 3.94QYY307 pKa = 10.62NAEE310 pKa = 4.23TGDD313 pKa = 3.8GSGTAVDD320 pKa = 3.66IAFAANGEE328 pKa = 4.4QVSLAQTAGDD338 pKa = 3.51ITVDD342 pKa = 3.03IPQAGEE348 pKa = 3.86CTVRR352 pKa = 11.84LNLYY356 pKa = 10.25NPTNCVVSVEE366 pKa = 4.59AGASTLPGGEE376 pKa = 4.38DD377 pKa = 3.38PGEE380 pKa = 4.11EE381 pKa = 4.29TPVTLPEE388 pKa = 4.28SLDD391 pKa = 3.45VVSYY395 pKa = 9.18STGSEE400 pKa = 4.04VLLTTLYY407 pKa = 8.0PTGSGSGVYY416 pKa = 9.3TGTYY420 pKa = 8.77SGEE423 pKa = 3.86VRR425 pKa = 11.84DD426 pKa = 4.36QINIVDD432 pKa = 5.22RR433 pKa = 11.84INSVWYY439 pKa = 10.02GCAPDD444 pKa = 5.6DD445 pKa = 4.31NSQLSSQKK453 pKa = 10.68SKK455 pKa = 10.09WNIWFDD461 pKa = 3.53GSGEE465 pKa = 4.08LTLTVDD471 pKa = 4.67LTNMTWNYY479 pKa = 7.19TANN482 pKa = 3.47

Molecular weight: 51.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5V6A6|R5V6A6_9BACE Dihydroorotate oxidase OS=Bacteroides plebeius CAG:211 OX=1263052 GN=BN536_01014 PE=4 SV=1
MM1 pKa = 7.72CGFFLANFPCWIDD14 pKa = 4.5FYY16 pKa = 10.85PLQCALFSLFGKK28 pKa = 8.53KK29 pKa = 6.51TTQKK33 pKa = 9.93FADD36 pKa = 3.72YY37 pKa = 10.72KK38 pKa = 10.18FSCTFATAIEE48 pKa = 4.25NEE50 pKa = 4.58TIGRR54 pKa = 11.84LAQLVQSICLTSRR67 pKa = 11.84GSAVRR72 pKa = 11.84IRR74 pKa = 11.84QRR76 pKa = 11.84PLNEE80 pKa = 3.74FLVNHH85 pKa = 6.54CFFFLGRR92 pKa = 11.84LAQLVQSICLTSRR105 pKa = 11.84GSAVRR110 pKa = 11.84IRR112 pKa = 11.84QRR114 pKa = 11.84PHH116 pKa = 6.86RR117 pKa = 11.84NIIVNFPPIGRR128 pKa = 11.84LAQLVQSICLTSRR141 pKa = 11.84GSAVRR146 pKa = 11.84IRR148 pKa = 11.84QRR150 pKa = 11.84PLQASQFCGAFFFF163 pKa = 5.44

Molecular weight: 18.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2643

0

2643

961016

29

4860

363.6

41.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.211 ± 0.047

1.343 ± 0.017

5.345 ± 0.033

6.84 ± 0.047

4.541 ± 0.029

7.031 ± 0.042

2.015 ± 0.02

6.336 ± 0.045

6.42 ± 0.041

9.29 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.857 ± 0.023

4.773 ± 0.04

3.769 ± 0.022

3.693 ± 0.031

4.678 ± 0.031

5.95 ± 0.038

5.525 ± 0.033

6.708 ± 0.038

1.296 ± 0.019

4.379 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski