Bacillus sp. JCM 19045

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4678 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W7ZC55|W7ZC55_9BACI Response regulator aspartate phosphatase OS=Bacillus sp. JCM 19045 OX=1460639 GN=JCM19045_3380 PE=3 SV=1
MM1 pKa = 7.37KK2 pKa = 10.53KK3 pKa = 10.35SMEE6 pKa = 4.15YY7 pKa = 11.1GLVSTMAIALLAGCNQGSSGGEE29 pKa = 3.69DD30 pKa = 3.25VVEE33 pKa = 4.66LSFSAWGNPAEE44 pKa = 4.04LEE46 pKa = 4.45VYY48 pKa = 10.2NRR50 pKa = 11.84AVDD53 pKa = 3.81AFNEE57 pKa = 4.25EE58 pKa = 3.61QDD60 pKa = 4.15EE61 pKa = 4.42IQVTLTGIPNDD72 pKa = 3.79NYY74 pKa = 10.55FQTLTTRR81 pKa = 11.84LQGGQAPDD89 pKa = 3.18VFYY92 pKa = 11.31VGSEE96 pKa = 4.01NMSTLIANGTIEE108 pKa = 4.19PMSDD112 pKa = 3.53FMGTDD117 pKa = 3.05EE118 pKa = 6.08SYY120 pKa = 10.21VQEE123 pKa = 5.02SEE125 pKa = 4.11FTDD128 pKa = 5.63DD129 pKa = 2.61IWGPSKK135 pKa = 10.58QDD137 pKa = 3.43EE138 pKa = 4.56IVYY141 pKa = 9.53GLPVDD146 pKa = 4.31CNPILMYY153 pKa = 10.6YY154 pKa = 10.61NKK156 pKa = 9.55TLLEE160 pKa = 4.39DD161 pKa = 3.95AGIEE165 pKa = 4.19SPQTYY170 pKa = 9.61FEE172 pKa = 4.13QGDD175 pKa = 4.1WNWDD179 pKa = 3.17TFAEE183 pKa = 4.29ITGKK187 pKa = 10.5LKK189 pKa = 10.4EE190 pKa = 4.62AGNHH194 pKa = 6.06GYY196 pKa = 8.91VQEE199 pKa = 4.58SGHH202 pKa = 6.29GNLINWVWTNGGNFATDD219 pKa = 3.55DD220 pKa = 4.93AIVADD225 pKa = 4.19TDD227 pKa = 3.79EE228 pKa = 4.5LTLEE232 pKa = 3.94AFEE235 pKa = 4.88YY236 pKa = 9.9VDD238 pKa = 4.74EE239 pKa = 4.4MVQAGNFTYY248 pKa = 10.57AGTMPDD254 pKa = 3.34GQGIEE259 pKa = 4.35GMFMTNSVGFVSAGRR274 pKa = 11.84WLTPMFLQTSLDD286 pKa = 3.3FDD288 pKa = 5.0YY289 pKa = 11.26IPFPSNTGEE298 pKa = 4.42PIEE301 pKa = 4.25TATIATAYY309 pKa = 7.96MAANSKK315 pKa = 10.38SEE317 pKa = 4.15HH318 pKa = 6.27VEE320 pKa = 3.71EE321 pKa = 4.18AQKK324 pKa = 10.76FISYY328 pKa = 6.46YY329 pKa = 10.46TSMEE333 pKa = 4.04GQRR336 pKa = 11.84VRR338 pKa = 11.84LDD340 pKa = 3.55GEE342 pKa = 4.63GNAVPSVEE350 pKa = 4.54GIDD353 pKa = 5.7DD354 pKa = 3.48IVLNQDD360 pKa = 3.29RR361 pKa = 11.84PEE363 pKa = 4.52HH364 pKa = 5.43GQYY367 pKa = 11.25LLDD370 pKa = 3.88ARR372 pKa = 11.84SVGRR376 pKa = 11.84VINYY380 pKa = 7.6EE381 pKa = 3.93FTSPGLNNDD390 pKa = 3.0LDD392 pKa = 4.55EE393 pKa = 6.08IYY395 pKa = 11.08EE396 pKa = 4.08LMLLGDD402 pKa = 3.82MTGEE406 pKa = 4.03EE407 pKa = 4.55ALDD410 pKa = 3.63EE411 pKa = 4.43ASRR414 pKa = 11.84VAKK417 pKa = 10.39AALDD421 pKa = 3.78EE422 pKa = 4.4

Molecular weight: 46.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W7Z1D5|W7Z1D5_9BACI GMP synthase (glutamine-hydrolyzing) OS=Bacillus sp. JCM 19045 OX=1460639 GN=JCM19045_4088 PE=4 SV=1
MM1 pKa = 7.61GPTFQPNNRR10 pKa = 11.84KK11 pKa = 9.34RR12 pKa = 11.84KK13 pKa = 8.16KK14 pKa = 9.1NHH16 pKa = 4.73GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37NGRR28 pKa = 11.84KK29 pKa = 8.82VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.39GRR39 pKa = 11.84KK40 pKa = 8.52VLSAA44 pKa = 4.05

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4678

0

4678

1234247

37

1642

263.8

29.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.78 ± 0.042

0.703 ± 0.011

5.084 ± 0.035

7.462 ± 0.053

4.582 ± 0.029

6.807 ± 0.041

2.22 ± 0.019

7.056 ± 0.037

5.609 ± 0.036

10.24 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.756 ± 0.02

3.993 ± 0.026

3.579 ± 0.019

4.264 ± 0.025

4.27 ± 0.024

6.2 ± 0.027

5.825 ± 0.032

7.083 ± 0.032

1.086 ± 0.016

3.4 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski