Amino acid dipepetide frequency for Shewanella sp. phage 1/40

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.252AlaAla: 4.252 ± 0.488
0.628AlaCys: 0.628 ± 0.125
3.527AlaAsp: 3.527 ± 0.288
3.744AlaGlu: 3.744 ± 0.317
1.788AlaPhe: 1.788 ± 0.227
4.227AlaGly: 4.227 ± 0.355
1.135AlaHis: 1.135 ± 0.177
3.648AlaIle: 3.648 ± 0.278
3.841AlaLys: 3.841 ± 0.356
4.058AlaLeu: 4.058 ± 0.32
1.522AlaMet: 1.522 ± 0.221
3.285AlaAsn: 3.285 ± 0.299
1.619AlaPro: 1.619 ± 0.207
2.005AlaGln: 2.005 ± 0.207
2.029AlaArg: 2.029 ± 0.209
3.454AlaSer: 3.454 ± 0.332
3.43AlaThr: 3.43 ± 0.293
4.01AlaVal: 4.01 ± 0.331
0.676AlaTrp: 0.676 ± 0.128
2.174AlaTyr: 2.174 ± 0.231
0.0AlaXaa: 0.0 ± 0.0
Cys
0.725CysAla: 0.725 ± 0.129
0.338CysCys: 0.338 ± 0.087
1.111CysAsp: 1.111 ± 0.18
1.039CysGlu: 1.039 ± 0.16
0.894CysPhe: 0.894 ± 0.153
1.401CysGly: 1.401 ± 0.176
0.314CysHis: 0.314 ± 0.088
0.821CysIle: 0.821 ± 0.172
0.99CysLys: 0.99 ± 0.16
1.256CysLeu: 1.256 ± 0.196
0.435CysMet: 0.435 ± 0.103
0.797CysAsn: 0.797 ± 0.169
0.29CysPro: 0.29 ± 0.092
0.435CysGln: 0.435 ± 0.092
0.676CysArg: 0.676 ± 0.134
1.087CysSer: 1.087 ± 0.18
0.845CysThr: 0.845 ± 0.156
1.015CysVal: 1.015 ± 0.174
0.314CysTrp: 0.314 ± 0.095
0.604CysTyr: 0.604 ± 0.111
0.0CysXaa: 0.0 ± 0.0
Asp
3.454AspAla: 3.454 ± 0.249
1.015AspCys: 1.015 ± 0.17
3.672AspAsp: 3.672 ± 0.311
4.131AspGlu: 4.131 ± 0.302
2.923AspPhe: 2.923 ± 0.276
5.097AspGly: 5.097 ± 0.375
1.063AspHis: 1.063 ± 0.164
4.493AspIle: 4.493 ± 0.452
5.194AspLys: 5.194 ± 0.411
5.653AspLeu: 5.653 ± 0.422
1.908AspMet: 1.908 ± 0.265
3.913AspAsn: 3.913 ± 0.309
1.667AspPro: 1.667 ± 0.194
1.546AspGln: 1.546 ± 0.183
1.908AspArg: 1.908 ± 0.203
4.058AspSer: 4.058 ± 0.31
4.614AspThr: 4.614 ± 0.414
4.252AspVal: 4.252 ± 0.319
1.546AspTrp: 1.546 ± 0.202
3.068AspTyr: 3.068 ± 0.252
0.0AspXaa: 0.0 ± 0.0
Glu
3.551GluAla: 3.551 ± 0.311
1.28GluCys: 1.28 ± 0.158
4.445GluAsp: 4.445 ± 0.363
4.397GluGlu: 4.397 ± 0.345
2.416GluPhe: 2.416 ± 0.204
4.227GluGly: 4.227 ± 0.38
1.353GluHis: 1.353 ± 0.173
4.348GluIle: 4.348 ± 0.326
3.744GluLys: 3.744 ± 0.315
5.532GluLeu: 5.532 ± 0.507
1.619GluMet: 1.619 ± 0.19
2.778GluAsn: 2.778 ± 0.263
1.522GluPro: 1.522 ± 0.212
1.957GluGln: 1.957 ± 0.237
2.319GluArg: 2.319 ± 0.295
3.768GluSer: 3.768 ± 0.308
3.189GluThr: 3.189 ± 0.274
5.967GluVal: 5.967 ± 0.392
1.015GluTrp: 1.015 ± 0.138
3.116GluTyr: 3.116 ± 0.282
0.0GluXaa: 0.0 ± 0.0
Phe
2.005PheAla: 2.005 ± 0.207
0.459PheCys: 0.459 ± 0.098
2.343PheAsp: 2.343 ± 0.234
2.633PheGlu: 2.633 ± 0.278
1.57PhePhe: 1.57 ± 0.239
2.536PheGly: 2.536 ± 0.337
0.749PheHis: 0.749 ± 0.131
2.826PheIle: 2.826 ± 0.24
3.261PheLys: 3.261 ± 0.286
3.14PheLeu: 3.14 ± 0.309
1.329PheMet: 1.329 ± 0.179
3.189PheAsn: 3.189 ± 0.306
1.135PhePro: 1.135 ± 0.18
1.135PheGln: 1.135 ± 0.145
1.329PheArg: 1.329 ± 0.192
3.454PheSer: 3.454 ± 0.316
2.971PheThr: 2.971 ± 0.3
2.271PheVal: 2.271 ± 0.233
0.362PheTrp: 0.362 ± 0.085
1.643PheTyr: 1.643 ± 0.208
0.0PheXaa: 0.0 ± 0.0
Gly
3.599GlyAla: 3.599 ± 0.282
0.99GlyCys: 0.99 ± 0.183
5.049GlyAsp: 5.049 ± 0.396
4.083GlyGlu: 4.083 ± 0.339
3.334GlyPhe: 3.334 ± 0.287
5.387GlyGly: 5.387 ± 0.382
1.184GlyHis: 1.184 ± 0.189
3.986GlyIle: 3.986 ± 0.308
4.88GlyLys: 4.88 ± 0.367
5.58GlyLeu: 5.58 ± 0.405
1.474GlyMet: 1.474 ± 0.177
3.913GlyAsn: 3.913 ± 0.315
0.266GlyPro: 0.266 ± 0.172
1.522GlyGln: 1.522 ± 0.195
2.198GlyArg: 2.198 ± 0.215
5.073GlySer: 5.073 ± 0.333
4.252GlyThr: 4.252 ± 0.395
6.184GlyVal: 6.184 ± 0.45
1.135GlyTrp: 1.135 ± 0.148
3.261GlyTyr: 3.261 ± 0.313
0.0GlyXaa: 0.0 ± 0.0
His
1.015HisAla: 1.015 ± 0.156
0.459HisCys: 0.459 ± 0.093
1.111HisAsp: 1.111 ± 0.199
1.16HisGlu: 1.16 ± 0.18
0.894HisPhe: 0.894 ± 0.147
1.256HisGly: 1.256 ± 0.168
0.58HisHis: 0.58 ± 0.105
1.401HisIle: 1.401 ± 0.157
1.401HisLys: 1.401 ± 0.211
1.135HisLeu: 1.135 ± 0.166
0.531HisMet: 0.531 ± 0.112
1.232HisAsn: 1.232 ± 0.157
0.652HisPro: 0.652 ± 0.102
0.749HisGln: 0.749 ± 0.124
0.725HisArg: 0.725 ± 0.134
1.16HisSer: 1.16 ± 0.147
1.691HisThr: 1.691 ± 0.211
1.256HisVal: 1.256 ± 0.162
0.387HisTrp: 0.387 ± 0.097
0.845HisTyr: 0.845 ± 0.145
0.0HisXaa: 0.0 ± 0.0
Ile
3.382IleAla: 3.382 ± 0.265
0.676IleCys: 0.676 ± 0.116
4.807IleAsp: 4.807 ± 0.357
4.711IleGlu: 4.711 ± 0.333
1.812IlePhe: 1.812 ± 0.203
4.01IleGly: 4.01 ± 0.365
1.087IleHis: 1.087 ± 0.165
3.938IleIle: 3.938 ± 0.352
5.073IleLys: 5.073 ± 0.396
4.88IleLeu: 4.88 ± 0.405
2.005IleMet: 2.005 ± 0.238
4.856IleAsn: 4.856 ± 0.426
2.512IlePro: 2.512 ± 0.258
1.788IleGln: 1.788 ± 0.248
2.609IleArg: 2.609 ± 0.306
4.445IleSer: 4.445 ± 0.342
5.339IleThr: 5.339 ± 0.48
3.865IleVal: 3.865 ± 0.273
0.676IleTrp: 0.676 ± 0.129
2.247IleTyr: 2.247 ± 0.179
0.0IleXaa: 0.0 ± 0.0
Lys
3.768LysAla: 3.768 ± 0.347
0.894LysCys: 0.894 ± 0.16
4.904LysAsp: 4.904 ± 0.38
4.445LysGlu: 4.445 ± 0.391
2.416LysPhe: 2.416 ± 0.238
4.542LysGly: 4.542 ± 0.367
1.522LysHis: 1.522 ± 0.236
3.889LysIle: 3.889 ± 0.345
3.841LysLys: 3.841 ± 0.433
6.595LysLeu: 6.595 ± 0.441
2.73LysMet: 2.73 ± 0.314
2.851LysAsn: 2.851 ± 0.251
2.464LysPro: 2.464 ± 0.266
3.116LysGln: 3.116 ± 0.416
3.624LysArg: 3.624 ± 0.331
4.469LysSer: 4.469 ± 0.373
4.324LysThr: 4.324 ± 0.335
6.353LysVal: 6.353 ± 0.436
1.111LysTrp: 1.111 ± 0.14
3.503LysTyr: 3.503 ± 0.315
0.0LysXaa: 0.0 ± 0.0
Leu
5.0LeuAla: 5.0 ± 0.324
1.425LeuCys: 1.425 ± 0.192
6.039LeuAsp: 6.039 ± 0.409
5.411LeuGlu: 5.411 ± 0.371
2.971LeuPhe: 2.971 ± 0.224
5.121LeuGly: 5.121 ± 0.33
1.86LeuHis: 1.86 ± 0.257
5.29LeuIle: 5.29 ± 0.353
6.136LeuLys: 6.136 ± 0.444
7.126LeuLeu: 7.126 ± 0.482
1.933LeuMet: 1.933 ± 0.194
4.928LeuAsn: 4.928 ± 0.387
3.817LeuPro: 3.817 ± 0.313
2.826LeuGln: 2.826 ± 0.255
3.044LeuArg: 3.044 ± 0.287
6.619LeuSer: 6.619 ± 0.383
5.749LeuThr: 5.749 ± 0.372
4.831LeuVal: 4.831 ± 0.37
1.063LeuTrp: 1.063 ± 0.161
3.575LeuTyr: 3.575 ± 0.294
0.0LeuXaa: 0.0 ± 0.0
Met
1.619MetAla: 1.619 ± 0.213
0.242MetCys: 0.242 ± 0.081
1.256MetAsp: 1.256 ± 0.16
1.546MetGlu: 1.546 ± 0.209
1.28MetPhe: 1.28 ± 0.169
1.667MetGly: 1.667 ± 0.196
0.387MetHis: 0.387 ± 0.102
1.474MetIle: 1.474 ± 0.216
2.609MetLys: 2.609 ± 0.277
2.875MetLeu: 2.875 ± 0.274
0.894MetMet: 0.894 ± 0.146
1.425MetAsn: 1.425 ± 0.192
0.845MetPro: 0.845 ± 0.123
0.918MetGln: 0.918 ± 0.152
1.015MetArg: 1.015 ± 0.157
2.561MetSer: 2.561 ± 0.248
1.715MetThr: 1.715 ± 0.181
1.86MetVal: 1.86 ± 0.192
0.435MetTrp: 0.435 ± 0.1
0.821MetTyr: 0.821 ± 0.141
0.0MetXaa: 0.0 ± 0.0
Asn
2.657AsnAla: 2.657 ± 0.359
0.773AsnCys: 0.773 ± 0.172
2.754AsnAsp: 2.754 ± 0.262
2.536AsnGlu: 2.536 ± 0.232
2.633AsnPhe: 2.633 ± 0.258
4.227AsnGly: 4.227 ± 0.376
1.111AsnHis: 1.111 ± 0.181
4.083AsnIle: 4.083 ± 0.434
5.194AsnLys: 5.194 ± 0.366
4.904AsnLeu: 4.904 ± 0.357
1.643AsnMet: 1.643 ± 0.215
4.034AsnAsn: 4.034 ± 0.343
2.488AsnPro: 2.488 ± 0.241
1.691AsnGln: 1.691 ± 0.199
2.44AsnArg: 2.44 ± 0.31
3.213AsnSer: 3.213 ± 0.29
5.049AsnThr: 5.049 ± 0.337
3.092AsnVal: 3.092 ± 0.312
0.676AsnTrp: 0.676 ± 0.137
2.609AsnTyr: 2.609 ± 0.232
0.0AsnXaa: 0.0 ± 0.0
Pro
1.933ProAla: 1.933 ± 0.252
0.483ProCys: 0.483 ± 0.123
2.174ProAsp: 2.174 ± 0.243
2.222ProGlu: 2.222 ± 0.259
1.449ProPhe: 1.449 ± 0.17
0.0ProGly: 0.0 ± 0.0
0.652ProHis: 0.652 ± 0.156
1.836ProIle: 1.836 ± 0.218
1.763ProLys: 1.763 ± 0.221
2.512ProLeu: 2.512 ± 0.246
0.797ProMet: 0.797 ± 0.149
1.691ProAsn: 1.691 ± 0.203
1.232ProPro: 1.232 ± 0.192
1.304ProGln: 1.304 ± 0.18
1.039ProArg: 1.039 ± 0.172
2.488ProSer: 2.488 ± 0.259
2.754ProThr: 2.754 ± 0.306
2.633ProVal: 2.633 ± 0.232
0.483ProTrp: 0.483 ± 0.107
1.353ProTyr: 1.353 ± 0.209
0.0ProXaa: 0.0 ± 0.0
Gln
1.836GlnAla: 1.836 ± 0.223
0.435GlnCys: 0.435 ± 0.097
1.908GlnAsp: 1.908 ± 0.242
2.295GlnGlu: 2.295 ± 0.226
1.449GlnPhe: 1.449 ± 0.187
1.836GlnGly: 1.836 ± 0.219
0.87GlnHis: 0.87 ± 0.157
1.715GlnIle: 1.715 ± 0.215
2.198GlnLys: 2.198 ± 0.261
2.73GlnLeu: 2.73 ± 0.232
1.087GlnMet: 1.087 ± 0.156
1.498GlnAsn: 1.498 ± 0.218
0.821GlnPro: 0.821 ± 0.166
1.594GlnGln: 1.594 ± 0.187
1.739GlnArg: 1.739 ± 0.208
2.198GlnSer: 2.198 ± 0.228
1.812GlnThr: 1.812 ± 0.232
2.271GlnVal: 2.271 ± 0.268
0.338GlnTrp: 0.338 ± 0.097
1.715GlnTyr: 1.715 ± 0.171
0.0GlnXaa: 0.0 ± 0.0
Arg
1.933ArgAla: 1.933 ± 0.241
0.797ArgCys: 0.797 ± 0.137
2.343ArgAsp: 2.343 ± 0.265
2.44ArgGlu: 2.44 ± 0.213
1.498ArgPhe: 1.498 ± 0.199
2.609ArgGly: 2.609 ± 0.263
0.701ArgHis: 0.701 ± 0.136
2.561ArgIle: 2.561 ± 0.237
3.213ArgLys: 3.213 ± 0.347
3.72ArgLeu: 3.72 ± 0.308
1.111ArgMet: 1.111 ± 0.167
1.884ArgAsn: 1.884 ± 0.211
1.135ArgPro: 1.135 ± 0.151
1.57ArgGln: 1.57 ± 0.236
1.353ArgArg: 1.353 ± 0.181
2.536ArgSer: 2.536 ± 0.235
1.812ArgThr: 1.812 ± 0.204
3.213ArgVal: 3.213 ± 0.287
0.362ArgTrp: 0.362 ± 0.111
1.739ArgTyr: 1.739 ± 0.224
0.0ArgXaa: 0.0 ± 0.0
Ser
3.454SerAla: 3.454 ± 0.276
1.184SerCys: 1.184 ± 0.168
4.711SerAsp: 4.711 ± 0.327
3.817SerGlu: 3.817 ± 0.306
2.947SerPhe: 2.947 ± 0.266
5.049SerGly: 5.049 ± 0.407
1.377SerHis: 1.377 ± 0.183
4.928SerIle: 4.928 ± 0.341
4.904SerLys: 4.904 ± 0.296
5.701SerLeu: 5.701 ± 0.407
1.619SerMet: 1.619 ± 0.237
3.696SerAsn: 3.696 ± 0.278
2.174SerPro: 2.174 ± 0.213
1.981SerGln: 1.981 ± 0.226
3.092SerArg: 3.092 ± 0.269
4.807SerSer: 4.807 ± 0.441
4.107SerThr: 4.107 ± 0.387
5.049SerVal: 5.049 ± 0.356
0.821SerTrp: 0.821 ± 0.14
2.826SerTyr: 2.826 ± 0.299
0.0SerXaa: 0.0 ± 0.0
Thr
3.793ThrAla: 3.793 ± 0.383
0.821ThrCys: 0.821 ± 0.154
3.672ThrAsp: 3.672 ± 0.333
3.503ThrGlu: 3.503 ± 0.286
2.995ThrPhe: 2.995 ± 0.294
4.952ThrGly: 4.952 ± 0.383
1.353ThrHis: 1.353 ± 0.18
4.88ThrIle: 4.88 ± 0.427
4.807ThrLys: 4.807 ± 0.494
6.112ThrLeu: 6.112 ± 0.422
1.28ThrMet: 1.28 ± 0.19
3.938ThrAsn: 3.938 ± 0.417
2.923ThrPro: 2.923 ± 0.297
2.198ThrGln: 2.198 ± 0.267
2.343ThrArg: 2.343 ± 0.301
4.662ThrSer: 4.662 ± 0.324
5.363ThrThr: 5.363 ± 0.559
4.686ThrVal: 4.686 ± 0.404
0.918ThrTrp: 0.918 ± 0.165
2.657ThrTyr: 2.657 ± 0.277
0.0ThrXaa: 0.0 ± 0.0
Val
4.276ValAla: 4.276 ± 0.331
1.111ValCys: 1.111 ± 0.159
5.411ValAsp: 5.411 ± 0.334
4.88ValGlu: 4.88 ± 0.391
2.512ValPhe: 2.512 ± 0.262
5.266ValGly: 5.266 ± 0.423
1.304ValHis: 1.304 ± 0.179
4.952ValIle: 4.952 ± 0.33
4.662ValLys: 4.662 ± 0.413
5.774ValLeu: 5.774 ± 0.391
2.005ValMet: 2.005 ± 0.234
4.517ValAsn: 4.517 ± 0.305
1.933ValPro: 1.933 ± 0.233
1.933ValGln: 1.933 ± 0.201
2.754ValArg: 2.754 ± 0.255
4.542ValSer: 4.542 ± 0.329
4.783ValThr: 4.783 ± 0.438
5.121ValVal: 5.121 ± 0.346
1.135ValTrp: 1.135 ± 0.179
3.43ValTyr: 3.43 ± 0.296
0.0ValXaa: 0.0 ± 0.0
Trp
0.58TrpAla: 0.58 ± 0.121
0.435TrpCys: 0.435 ± 0.099
1.232TrpAsp: 1.232 ± 0.184
0.99TrpGlu: 0.99 ± 0.175
0.894TrpPhe: 0.894 ± 0.186
0.773TrpGly: 0.773 ± 0.155
0.145TrpHis: 0.145 ± 0.059
0.652TrpIle: 0.652 ± 0.115
0.99TrpLys: 0.99 ± 0.172
1.594TrpLeu: 1.594 ± 0.182
0.314TrpMet: 0.314 ± 0.082
0.58TrpAsn: 0.58 ± 0.116
0.169TrpPro: 0.169 ± 0.07
0.387TrpGln: 0.387 ± 0.092
0.701TrpArg: 0.701 ± 0.138
0.773TrpSer: 0.773 ± 0.128
0.749TrpThr: 0.749 ± 0.133
1.425TrpVal: 1.425 ± 0.189
0.242TrpTrp: 0.242 ± 0.071
0.725TrpTyr: 0.725 ± 0.124
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.222TyrAla: 2.222 ± 0.263
0.942TyrCys: 0.942 ± 0.121
2.706TyrAsp: 2.706 ± 0.268
2.561TyrGlu: 2.561 ± 0.257
1.667TyrPhe: 1.667 ± 0.193
3.165TyrGly: 3.165 ± 0.265
0.894TyrHis: 0.894 ± 0.159
2.995TyrIle: 2.995 ± 0.29
2.657TyrLys: 2.657 ± 0.269
3.913TyrLeu: 3.913 ± 0.294
1.184TyrMet: 1.184 ± 0.158
2.802TyrAsn: 2.802 ± 0.295
1.135TyrPro: 1.135 ± 0.184
1.691TyrGln: 1.691 ± 0.191
1.594TyrArg: 1.594 ± 0.179
2.875TyrSer: 2.875 ± 0.262
3.309TyrThr: 3.309 ± 0.332
2.947TyrVal: 2.947 ± 0.272
0.652TyrTrp: 0.652 ± 0.126
2.077TyrTyr: 2.077 ± 0.237
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 236 proteins (41397 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski