Grapevine satellite virus

Taxonomy: Viruses; Riboviria; Virtovirus; Tobacco necrosis satellite virus-like

Average proteome isoelectric point is 8.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9UEY1|R9UEY1_9VIRU ORF2 OS=Grapevine satellite virus OX=1343493 PE=4 SV=1
MM1 pKa = 7.68KK2 pKa = 10.45DD3 pKa = 3.15LVFFMRR9 pKa = 11.84KK10 pKa = 8.11VNFVDD15 pKa = 3.84DD16 pKa = 4.12DD17 pKa = 4.61VYY19 pKa = 11.66DD20 pKa = 3.93FVMAHH25 pKa = 4.91YY26 pKa = 10.21VSWGWYY32 pKa = 8.23GGNIGITFDD41 pKa = 4.27FGNKK45 pKa = 9.4CKK47 pKa = 10.55EE48 pKa = 3.8FLNRR52 pKa = 11.84VTDD55 pKa = 4.48PSAVSNKK62 pKa = 9.28FSQLIAFSRR71 pKa = 11.84LCDD74 pKa = 3.45TNSIKK79 pKa = 11.06NKK81 pKa = 9.56IIVRR85 pKa = 11.84NFDD88 pKa = 3.42LMM90 pKa = 4.67

Molecular weight: 10.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9UEY1|R9UEY1_9VIRU ORF2 OS=Grapevine satellite virus OX=1343493 PE=4 SV=1
MM1 pKa = 7.33VNRR4 pKa = 11.84RR5 pKa = 11.84PRR7 pKa = 11.84NTTRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84GVPALRR19 pKa = 11.84EE20 pKa = 3.91VNYY23 pKa = 9.17ATVNAGTQTAFARR36 pKa = 11.84KK37 pKa = 9.29DD38 pKa = 3.52LQILAGCGDD47 pKa = 3.73RR48 pKa = 11.84SFKK51 pKa = 10.95LVGLALQVSSLSEE64 pKa = 3.66PVIVQIHH71 pKa = 6.42IFNEE75 pKa = 3.91ALKK78 pKa = 10.64EE79 pKa = 3.71IALCNRR85 pKa = 11.84LVAQGNTWLRR95 pKa = 11.84LRR97 pKa = 11.84IPLSYY102 pKa = 9.48KK103 pKa = 9.12QWWSGEE109 pKa = 4.23TTQSQTLVRR118 pKa = 11.84VDD120 pKa = 4.76IIPTYY125 pKa = 10.68KK126 pKa = 10.43NQNTKK131 pKa = 10.29VSFLLSLLCRR141 pKa = 11.84LGTSEE146 pKa = 4.15ITAHH150 pKa = 6.37PP151 pKa = 3.97

Molecular weight: 16.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

241

90

151

120.5

13.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.809 ± 1.438

2.075 ± 0.086

4.979 ± 2.916

2.905 ± 1.042

5.809 ± 3.08

5.394 ± 0.094

1.245 ± 0.078

6.224 ± 0.257

5.394 ± 1.385

9.959 ± 2.558

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.075 ± 1.377

7.054 ± 1.066

2.905 ± 1.042

4.149 ± 1.765

7.469 ± 1.757

6.639 ± 0.016

6.639 ± 1.92

8.714 ± 0.747

2.075 ± 0.086

2.49 ± 0.49
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski