Pseudomonas phage PPPL-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Ghunavirus; Pseudomonas virus PPPL1

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S2MVP1|A0A0S2MVP1_9CAUD Uncharacterized protein OS=Pseudomonas phage PPPL-1 OX=1755692 GN=PPPL1_032 PE=4 SV=1
MM1 pKa = 7.37LAIIQHH7 pKa = 6.16YY8 pKa = 8.81IHH10 pKa = 6.86NPDD13 pKa = 5.77DD14 pKa = 4.08IPDD17 pKa = 3.72IPVNSATYY25 pKa = 10.99LNVRR29 pKa = 11.84LNASYY34 pKa = 10.85LIATGAVDD42 pKa = 3.45EE43 pKa = 4.45LRR45 pKa = 11.84RR46 pKa = 11.84GGMSEE51 pKa = 3.86QYY53 pKa = 10.14IAGFLEE59 pKa = 4.58GCNAATEE66 pKa = 4.68IIEE69 pKa = 4.9LMQEE73 pKa = 3.97SQRR76 pKa = 11.84QQEE79 pKa = 4.32EE80 pKa = 4.06

Molecular weight: 8.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S2MVL9|A0A0S2MVL9_9CAUD Uncharacterized protein OS=Pseudomonas phage PPPL-1 OX=1755692 GN=PPPL1_016 PE=4 SV=1
MM1 pKa = 7.37VRR3 pKa = 11.84EE4 pKa = 4.68GGLKK8 pKa = 8.44TLTVATHH15 pKa = 6.31TPSTTWRR22 pKa = 11.84ALNDD26 pKa = 3.77PFTQQQRR33 pKa = 11.84HH34 pKa = 4.49WALHH38 pKa = 5.87RR39 pKa = 11.84SQPTRR44 pKa = 11.84DD45 pKa = 2.94RR46 pKa = 11.84GVARR50 pKa = 11.84QEE52 pKa = 3.56AHH54 pKa = 6.48EE55 pKa = 4.66RR56 pKa = 11.84DD57 pKa = 3.66PSRR60 pKa = 11.84SRR62 pKa = 11.84KK63 pKa = 9.72GDD65 pKa = 3.47LSGHH69 pKa = 5.07QEE71 pKa = 3.73HH72 pKa = 7.05LRR74 pKa = 11.84RR75 pKa = 11.84FPKK78 pKa = 10.56AGASCSEE85 pKa = 4.23TVHH88 pKa = 6.06RR89 pKa = 11.84QVAALCSGLEE99 pKa = 3.86DD100 pKa = 4.23RR101 pKa = 11.84EE102 pKa = 4.58GVRR105 pKa = 11.84QQ106 pKa = 3.43

Molecular weight: 12.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

12750

45

1400

260.2

28.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.867 ± 0.443

0.847 ± 0.149

6.549 ± 0.282

6.737 ± 0.409

3.631 ± 0.191

7.859 ± 0.328

2.071 ± 0.217

4.886 ± 0.195

5.89 ± 0.367

7.992 ± 0.32

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.659 ± 0.124

4.227 ± 0.367

3.875 ± 0.222

4.424 ± 0.285

5.867 ± 0.203

5.82 ± 0.369

5.71 ± 0.257

6.494 ± 0.318

1.467 ± 0.138

3.129 ± 0.168
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski