Escherichia phage EG1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Teseptimavirus; Escherichia virus EG1

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4P697|A0A2H4P697_9CAUD Uncharacterized protein OS=Escherichia phage EG1 OX=2053563 PE=4 SV=1
MM1 pKa = 7.48AMSNMTYY8 pKa = 11.18NNVFDD13 pKa = 4.72HH14 pKa = 6.93AYY16 pKa = 10.49EE17 pKa = 3.86MLKK20 pKa = 10.85EE21 pKa = 4.08NIRR24 pKa = 11.84YY25 pKa = 9.83DD26 pKa = 4.51DD27 pKa = 3.73IRR29 pKa = 11.84DD30 pKa = 3.47ADD32 pKa = 3.89GLHH35 pKa = 6.97DD36 pKa = 4.72AVHH39 pKa = 6.23MAADD43 pKa = 3.59AAVPHH48 pKa = 6.61YY49 pKa = 10.36YY50 pKa = 10.69SDD52 pKa = 3.44IFSVMASEE60 pKa = 5.55GIDD63 pKa = 4.5LEE65 pKa = 4.76FEE67 pKa = 4.73DD68 pKa = 5.02SGLMPDD74 pKa = 3.4TKK76 pKa = 10.94DD77 pKa = 3.64VIRR80 pKa = 11.84ILQARR85 pKa = 11.84IYY87 pKa = 9.25EE88 pKa = 4.14QLTIDD93 pKa = 3.64LWEE96 pKa = 4.69DD97 pKa = 3.57AEE99 pKa = 4.86DD100 pKa = 4.13LLNEE104 pKa = 3.98YY105 pKa = 10.49LEE107 pKa = 4.28EE108 pKa = 4.24VEE110 pKa = 4.59EE111 pKa = 4.42SEE113 pKa = 4.96EE114 pKa = 4.12YY115 pKa = 10.94EE116 pKa = 4.21EE117 pKa = 6.06DD118 pKa = 3.99GEE120 pKa = 4.4

Molecular weight: 13.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4P6E5|A0A2H4P6E5_9CAUD Terminase large subunit OS=Escherichia phage EG1 OX=2053563 PE=3 SV=1
MM1 pKa = 7.42LKK3 pKa = 10.27YY4 pKa = 10.69YY5 pKa = 10.62VMPIHH10 pKa = 6.55TKK12 pKa = 10.57NGATVCTPDD21 pKa = 3.38GFAMKK26 pKa = 10.26QRR28 pKa = 11.84IEE30 pKa = 3.89RR31 pKa = 11.84LKK33 pKa = 10.95RR34 pKa = 11.84EE35 pKa = 3.7LRR37 pKa = 11.84TNRR40 pKa = 11.84KK41 pKa = 9.14LNNII45 pKa = 4.0

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

12030

32

1324

235.9

26.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.244 ± 0.486

1.131 ± 0.203

6.534 ± 0.24

7.382 ± 0.358

3.549 ± 0.186

7.265 ± 0.304

1.978 ± 0.2

5.395 ± 0.193

6.633 ± 0.352

7.739 ± 0.322

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.984 ± 0.21

4.456 ± 0.277

3.441 ± 0.218

3.691 ± 0.238

5.769 ± 0.214

6.027 ± 0.314

5.278 ± 0.206

6.459 ± 0.246

1.588 ± 0.187

3.458 ± 0.188
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski