Amino acid dipepetide frequency for Streptomyces sp. NWU339

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.084AlaAla: 20.084 ± 0.144	1.098AlaCys: 1.098 ± 0.021	8.179AlaAsp: 8.179 ± 0.066	8.879AlaGlu: 8.879 ± 0.082	3.37AlaPhe: 3.37 ± 0.041	12.385AlaGly: 12.385 ± 0.085	2.915AlaHis: 2.915 ± 0.041	3.256AlaIle: 3.256 ± 0.042	2.751AlaLys: 2.751 ± 0.042	13.999AlaLeu: 13.999 ± 0.116	2.455AlaMet: 2.455 ± 0.036	1.836AlaAsn: 1.836 ± 0.026	7.16AlaPro: 7.16 ± 0.08	3.761AlaGln: 3.761 ± 0.038	10.537AlaArg: 10.537 ± 0.094	5.749AlaSer: 5.749 ± 0.06	6.855AlaThr: 6.855 ± 0.058	12.065AlaVal: 12.065 ± 0.086	1.837AlaTrp: 1.837 ± 0.027	2.623AlaTyr: 2.623 ± 0.033	0.016AlaXaa: 0.016 ± 0.003
Cys	1.107CysAla: 1.107 ± 0.024	0.103CysCys: 0.103 ± 0.008	0.507CysAsp: 0.507 ± 0.018	0.441CysGlu: 0.441 ± 0.013	0.221CysPhe: 0.221 ± 0.01	0.984CysGly: 0.984 ± 0.023	0.211CysHis: 0.211 ± 0.01	0.162CysIle: 0.162 ± 0.007	0.112CysLys: 0.112 ± 0.007	0.778CysLeu: 0.778 ± 0.018	0.13CysMet: 0.13 ± 0.007	0.134CysAsn: 0.134 ± 0.006	0.537CysPro: 0.537 ± 0.016	0.165CysGln: 0.165 ± 0.007	0.706CysArg: 0.706 ± 0.018	0.471CysSer: 0.471 ± 0.016	0.526CysThr: 0.526 ± 0.014	0.675CysVal: 0.675 ± 0.016	0.154CysTrp: 0.154 ± 0.007	0.153CysTyr: 0.153 ± 0.008	0.002CysXaa: 0.002 ± 0.001
Asp	7.478AspAla: 7.478 ± 0.069	0.444AspCys: 0.444 ± 0.016	3.727AspAsp: 3.727 ± 0.055	3.981AspGlu: 3.981 ± 0.049	1.645AspPhe: 1.645 ± 0.026	6.383AspGly: 6.383 ± 0.062	1.497AspHis: 1.497 ± 0.025	1.977AspIle: 1.977 ± 0.035	1.214AspLys: 1.214 ± 0.025	6.164AspLeu: 6.164 ± 0.057	0.84AspMet: 0.84 ± 0.02	0.97AspAsn: 0.97 ± 0.021	4.476AspPro: 4.476 ± 0.046	1.568AspGln: 1.568 ± 0.027	5.252AspArg: 5.252 ± 0.056	2.602AspSer: 2.602 ± 0.031	3.327AspThr: 3.327 ± 0.039	4.689AspVal: 4.689 ± 0.047	1.01AspTrp: 1.01 ± 0.023	1.061AspTyr: 1.061 ± 0.02	0.003AspXaa: 0.003 ± 0.001
Glu	7.481GluAla: 7.481 ± 0.071	0.39GluCys: 0.39 ± 0.013	2.963GluAsp: 2.963 ± 0.037	3.864GluGlu: 3.864 ± 0.052	1.473GluPhe: 1.473 ± 0.027	4.485GluGly: 4.485 ± 0.043	1.64GluHis: 1.64 ± 0.027	2.307GluIle: 2.307 ± 0.033	1.564GluLys: 1.564 ± 0.031	6.8GluLeu: 6.8 ± 0.063	0.938GluMet: 0.938 ± 0.02	1.124GluAsn: 1.124 ± 0.024	3.563GluPro: 3.563 ± 0.04	2.473GluGln: 2.473 ± 0.037	5.747GluArg: 5.747 ± 0.055	2.609GluSer: 2.609 ± 0.037	3.06GluThr: 3.06 ± 0.038	4.638GluVal: 4.638 ± 0.053	0.816GluTrp: 0.816 ± 0.018	1.169GluTyr: 1.169 ± 0.024	0.005GluXaa: 0.005 ± 0.001
Phe	3.559PheAla: 3.559 ± 0.04	0.266PheCys: 0.266 ± 0.01	1.932PheAsp: 1.932 ± 0.034	1.457PheGlu: 1.457 ± 0.028	0.835PhePhe: 0.835 ± 0.024	2.916PheGly: 2.916 ± 0.035	0.629PheHis: 0.629 ± 0.018	0.684PheIle: 0.684 ± 0.017	0.517PheLys: 0.517 ± 0.016	2.555PheLeu: 2.555 ± 0.038	0.386PheMet: 0.386 ± 0.014	0.53PheAsn: 0.53 ± 0.016	1.417PhePro: 1.417 ± 0.021	0.67PheGln: 0.67 ± 0.016	1.959PheArg: 1.959 ± 0.031	1.394PheSer: 1.394 ± 0.024	2.049PheThr: 2.049 ± 0.031	2.189PheVal: 2.189 ± 0.034	0.395PheTrp: 0.395 ± 0.014	0.543PheTyr: 0.543 ± 0.015	0.001PheXaa: 0.001 ± 0.001
Gly	10.673GlyAla: 10.673 ± 0.083	0.842GlyCys: 0.842 ± 0.022	5.126GlyAsp: 5.126 ± 0.049	5.264GlyGlu: 5.264 ± 0.048	2.81GlyPhe: 2.81 ± 0.04	8.664GlyGly: 8.664 ± 0.082	2.375GlyHis: 2.375 ± 0.033	3.388GlyIle: 3.388 ± 0.04	2.446GlyLys: 2.446 ± 0.035	9.15GlyLeu: 9.15 ± 0.068	1.957GlyMet: 1.957 ± 0.029	1.777GlyAsn: 1.777 ± 0.033	5.292GlyPro: 5.292 ± 0.055	2.706GlyGln: 2.706 ± 0.032	7.967GlyArg: 7.967 ± 0.064	5.26GlySer: 5.26 ± 0.059	6.4GlyThr: 6.4 ± 0.059	7.346GlyVal: 7.346 ± 0.055	1.652GlyTrp: 1.652 ± 0.027	2.196GlyTyr: 2.196 ± 0.035	0.014GlyXaa: 0.014 ± 0.002
His	2.73HisAla: 2.73 ± 0.035	0.239HisCys: 0.239 ± 0.01	1.431HisAsp: 1.431 ± 0.024	1.295HisGlu: 1.295 ± 0.024	0.694HisPhe: 0.694 ± 0.018	2.501HisGly: 2.501 ± 0.039	0.746HisHis: 0.746 ± 0.023	0.668HisIle: 0.668 ± 0.019	0.383HisLys: 0.383 ± 0.013	2.557HisLeu: 2.557 ± 0.031	0.339HisMet: 0.339 ± 0.011	0.366HisAsn: 0.366 ± 0.011	1.861HisPro: 1.861 ± 0.032	0.685HisGln: 0.685 ± 0.018	2.354HisArg: 2.354 ± 0.04	1.016HisSer: 1.016 ± 0.018	1.431HisThr: 1.431 ± 0.027	1.737HisVal: 1.737 ± 0.027	0.392HisTrp: 0.392 ± 0.012	0.46HisTyr: 0.46 ± 0.015	0.004HisXaa: 0.004 ± 0.001
Ile	4.561IleAla: 4.561 ± 0.052	0.301IleCys: 0.301 ± 0.012	2.265IleAsp: 2.265 ± 0.033	2.052IleGlu: 2.052 ± 0.033	0.655IlePhe: 0.655 ± 0.018	3.416IleGly: 3.416 ± 0.038	0.626IleHis: 0.626 ± 0.017	0.848IleIle: 0.848 ± 0.023	0.725IleLys: 0.725 ± 0.017	2.353IleLeu: 2.353 ± 0.031	0.441IleMet: 0.441 ± 0.013	0.705IleAsn: 0.705 ± 0.016	1.75IlePro: 1.75 ± 0.027	0.765IleGln: 0.765 ± 0.018	2.365IleArg: 2.365 ± 0.037	1.633IleSer: 1.633 ± 0.028	2.242IleThr: 2.242 ± 0.031	2.733IleVal: 2.733 ± 0.033	0.357IleTrp: 0.357 ± 0.013	0.473IleTyr: 0.473 ± 0.014	0.003IleXaa: 0.003 ± 0.001
Lys	2.902LysAla: 2.902 ± 0.041	0.119LysCys: 0.119 ± 0.007	1.356LysAsp: 1.356 ± 0.032	1.271LysGlu: 1.271 ± 0.025	0.469LysPhe: 0.469 ± 0.014	1.897LysGly: 1.897 ± 0.038	0.435LysHis: 0.435 ± 0.013	0.856LysIle: 0.856 ± 0.023	0.916LysLys: 0.916 ± 0.028	2.014LysLeu: 2.014 ± 0.035	0.348LysMet: 0.348 ± 0.012	0.546LysAsn: 0.546 ± 0.018	1.321LysPro: 1.321 ± 0.025	0.735LysGln: 0.735 ± 0.018	1.55LysArg: 1.55 ± 0.029	1.137LysSer: 1.137 ± 0.024	1.348LysThr: 1.348 ± 0.027	1.879LysVal: 1.879 ± 0.035	0.284LysTrp: 0.284 ± 0.01	0.44LysTyr: 0.44 ± 0.015	0.003LysXaa: 0.003 ± 0.002
Leu	14.429LeuAla: 14.429 ± 0.103	0.868LeuCys: 0.868 ± 0.022	6.644LeuAsp: 6.644 ± 0.056	5.037LeuGlu: 5.037 ± 0.056	2.528LeuPhe: 2.528 ± 0.038	8.891LeuGly: 8.891 ± 0.063	2.313LeuHis: 2.313 ± 0.032	3.222LeuIle: 3.222 ± 0.04	2.071LeuLys: 2.071 ± 0.034	11.221LeuLeu: 11.221 ± 0.104	1.627LeuMet: 1.627 ± 0.027	1.724LeuAsn: 1.724 ± 0.027	6.444LeuPro: 6.444 ± 0.06	2.221LeuGln: 2.221 ± 0.03	8.857LeuArg: 8.857 ± 0.076	5.275LeuSer: 5.275 ± 0.043	6.892LeuThr: 6.892 ± 0.06	8.689LeuVal: 8.689 ± 0.07	1.292LeuTrp: 1.292 ± 0.024	1.83LeuTyr: 1.83 ± 0.025	0.014LeuXaa: 0.014 ± 0.003
Met	2.201MetAla: 2.201 ± 0.033	0.138MetCys: 0.138 ± 0.006	0.85MetAsp: 0.85 ± 0.018	0.777MetGlu: 0.777 ± 0.018	0.454MetPhe: 0.454 ± 0.015	1.309MetGly: 1.309 ± 0.025	0.361MetHis: 0.361 ± 0.011	0.666MetIle: 0.666 ± 0.016	0.428MetLys: 0.428 ± 0.014	1.667MetLeu: 1.667 ± 0.027	0.293MetMet: 0.293 ± 0.013	0.439MetAsn: 0.439 ± 0.014	1.144MetPro: 1.144 ± 0.02	0.426MetGln: 0.426 ± 0.013	1.461MetArg: 1.461 ± 0.023	1.303MetSer: 1.303 ± 0.02	1.566MetThr: 1.566 ± 0.027	1.27MetVal: 1.27 ± 0.025	0.215MetTrp: 0.215 ± 0.01	0.317MetTyr: 0.317 ± 0.011	0.002MetXaa: 0.002 ± 0.001
Asn	2.174AsnAla: 2.174 ± 0.03	0.173AsnCys: 0.173 ± 0.008	0.953AsnAsp: 0.953 ± 0.02	0.851AsnGlu: 0.851 ± 0.016	0.462AsnPhe: 0.462 ± 0.013	1.89AsnGly: 1.89 ± 0.034	0.396AsnHis: 0.396 ± 0.013	0.621AsnIle: 0.621 ± 0.019	0.408AsnLys: 0.408 ± 0.014	1.651AsnLeu: 1.651 ± 0.034	0.295AsnMet: 0.295 ± 0.01	0.438AsnAsn: 0.438 ± 0.016	1.368AsnPro: 1.368 ± 0.023	0.539AsnGln: 0.539 ± 0.015	1.376AsnArg: 1.376 ± 0.024	0.923AsnSer: 0.923 ± 0.021	1.101AsnThr: 1.101 ± 0.023	1.335AsnVal: 1.335 ± 0.026	0.311AsnTrp: 0.311 ± 0.011	0.396AsnTyr: 0.396 ± 0.014	0.002AsnXaa: 0.002 ± 0.001
Pro	8.564ProAla: 8.564 ± 0.074	0.388ProCys: 0.388 ± 0.014	4.604ProAsp: 4.604 ± 0.047	4.554ProGlu: 4.554 ± 0.042	1.522ProPhe: 1.522 ± 0.025	6.823ProGly: 6.823 ± 0.07	1.479ProHis: 1.479 ± 0.028	1.242ProIle: 1.242 ± 0.027	1.201ProLys: 1.201 ± 0.025	5.329ProLeu: 5.329 ± 0.056	0.992ProMet: 0.992 ± 0.019	0.891ProAsn: 0.891 ± 0.021	3.756ProPro: 3.756 ± 0.059	1.799ProGln: 1.799 ± 0.029	4.228ProArg: 4.228 ± 0.047	3.297ProSer: 3.297 ± 0.044	3.332ProThr: 3.332 ± 0.039	5.568ProVal: 5.568 ± 0.055	0.9ProTrp: 0.9 ± 0.021	1.367ProTyr: 1.367 ± 0.027	0.008ProXaa: 0.008 ± 0.002
Gln	3.809GlnAla: 3.809 ± 0.049	0.187GlnCys: 0.187 ± 0.008	1.506GlnAsp: 1.506 ± 0.026	1.544GlnGlu: 1.544 ± 0.029	0.672GlnPhe: 0.672 ± 0.017	2.383GlnGly: 2.383 ± 0.035	0.691GlnHis: 0.691 ± 0.019	1.062GlnIle: 1.062 ± 0.024	0.641GlnLys: 0.641 ± 0.016	2.982GlnLeu: 2.982 ± 0.041	0.509GlnMet: 0.509 ± 0.015	0.521GlnAsn: 0.521 ± 0.016	1.736GlnPro: 1.736 ± 0.033	1.314GlnGln: 1.314 ± 0.033	2.51GlnArg: 2.51 ± 0.033	1.265GlnSer: 1.265 ± 0.023	1.35GlnThr: 1.35 ± 0.026	2.471GlnVal: 2.471 ± 0.033	0.463GlnTrp: 0.463 ± 0.015	0.612GlnTyr: 0.612 ± 0.015	0.003GlnXaa: 0.003 ± 0.002
Arg	10.033ArgAla: 10.033 ± 0.096	0.677ArgCys: 0.677 ± 0.019	4.466ArgAsp: 4.466 ± 0.051	4.997ArgGlu: 4.997 ± 0.05	2.408ArgPhe: 2.408 ± 0.036	6.093ArgGly: 6.093 ± 0.061	2.36ArgHis: 2.36 ± 0.034	3.309ArgIle: 3.309 ± 0.038	1.738ArgLys: 1.738 ± 0.028	9.169ArgLeu: 9.169 ± 0.069	1.823ArgMet: 1.823 ± 0.029	1.427ArgAsn: 1.427 ± 0.03	5.36ArgPro: 5.36 ± 0.052	2.489ArgGln: 2.489 ± 0.037	8.438ArgArg: 8.438 ± 0.08	4.285ArgSer: 4.285 ± 0.043	5.592ArgThr: 5.592 ± 0.052	6.096ArgVal: 6.096 ± 0.053	1.478ArgTrp: 1.478 ± 0.027	1.842ArgTyr: 1.842 ± 0.025	0.01ArgXaa: 0.01 ± 0.002
Ser	6.612SerAla: 6.612 ± 0.066	0.429SerCys: 0.429 ± 0.014	2.713SerAsp: 2.713 ± 0.035	2.519SerGlu: 2.519 ± 0.032	1.533SerPhe: 1.533 ± 0.025	5.853SerGly: 5.853 ± 0.06	1.073SerHis: 1.073 ± 0.023	1.396SerIle: 1.396 ± 0.024	1.033SerLys: 1.033 ± 0.023	4.717SerLeu: 4.717 ± 0.049	1.038SerMet: 1.038 ± 0.023	0.852SerAsn: 0.852 ± 0.021	3.231SerPro: 3.231 ± 0.041	1.232SerGln: 1.232 ± 0.024	3.88SerArg: 3.88 ± 0.04	2.837SerSer: 2.837 ± 0.043	3.097SerThr: 3.097 ± 0.042	4.234SerVal: 4.234 ± 0.051	0.868SerTrp: 0.868 ± 0.018	1.181SerTyr: 1.181 ± 0.026	0.006SerXaa: 0.006 ± 0.002
Thr	8.832ThrAla: 8.832 ± 0.072	0.481ThrCys: 0.481 ± 0.014	3.722ThrAsp: 3.722 ± 0.044	3.455ThrGlu: 3.455 ± 0.034	1.562ThrPhe: 1.562 ± 0.027	6.8ThrGly: 6.8 ± 0.062	1.274ThrHis: 1.274 ± 0.023	1.642ThrIle: 1.642 ± 0.029	1.143ThrLys: 1.143 ± 0.026	5.742ThrLeu: 5.742 ± 0.065	0.952ThrMet: 0.952 ± 0.022	0.958ThrAsn: 0.958 ± 0.02	4.204ThrPro: 4.204 ± 0.054	1.353ThrGln: 1.353 ± 0.027	4.24ThrArg: 4.24 ± 0.048	3.188ThrSer: 3.188 ± 0.041	3.875ThrThr: 3.875 ± 0.049	6.187ThrVal: 6.187 ± 0.06	0.93ThrTrp: 0.93 ± 0.02	1.343ThrTyr: 1.343 ± 0.029	0.005ThrXaa: 0.005 ± 0.002
Val	10.252ValAla: 10.252 ± 0.077	0.76ValCys: 0.76 ± 0.018	5.057ValAsp: 5.057 ± 0.046	4.826ValGlu: 4.826 ± 0.05	2.428ValPhe: 2.428 ± 0.035	6.405ValGly: 6.405 ± 0.056	2.047ValHis: 2.047 ± 0.03	2.905ValIle: 2.905 ± 0.037	1.764ValLys: 1.764 ± 0.03	9.451ValLeu: 9.451 ± 0.078	1.387ValMet: 1.387 ± 0.025	1.614ValAsn: 1.614 ± 0.028	5.371ValPro: 5.371 ± 0.057	2.12ValGln: 2.12 ± 0.025	7.435ValArg: 7.435 ± 0.059	4.214ValSer: 4.214 ± 0.041	5.651ValThr: 5.651 ± 0.05	7.919ValVal: 7.919 ± 0.071	1.169ValTrp: 1.169 ± 0.02	1.529ValTyr: 1.529 ± 0.025	0.006ValXaa: 0.006 ± 0.001
Trp	1.645TrpAla: 1.645 ± 0.028	0.18TrpCys: 0.18 ± 0.01	0.814TrpAsp: 0.814 ± 0.02	0.757TrpGlu: 0.757 ± 0.019	0.518TrpPhe: 0.518 ± 0.015	1.037TrpGly: 1.037 ± 0.021	0.4TrpHis: 0.4 ± 0.013	0.556TrpIle: 0.556 ± 0.017	0.377TrpLys: 0.377 ± 0.012	1.773TrpLeu: 1.773 ± 0.03	0.282TrpMet: 0.282 ± 0.011	0.426TrpAsn: 0.426 ± 0.015	0.805TrpPro: 0.805 ± 0.016	0.611TrpGln: 0.611 ± 0.018	1.384TrpArg: 1.384 ± 0.024	0.916TrpSer: 0.916 ± 0.021	1.036TrpThr: 1.036 ± 0.024	0.975TrpVal: 0.975 ± 0.02	0.346TrpTrp: 0.346 ± 0.012	0.342TrpTyr: 0.342 ± 0.013	0.002TrpXaa: 0.002 ± 0.001
Tyr	2.666TyrAla: 2.666 ± 0.032	0.195TyrCys: 0.195 ± 0.008	1.485TyrAsp: 1.485 ± 0.03	1.282TyrGlu: 1.282 ± 0.024	0.62TyrPhe: 0.62 ± 0.016	2.176TyrGly: 2.176 ± 0.031	0.422TyrHis: 0.422 ± 0.014	0.5TyrIle: 0.5 ± 0.015	0.391TyrLys: 0.391 ± 0.015	2.004TyrLeu: 2.004 ± 0.027	0.249TyrMet: 0.249 ± 0.011	0.384TyrAsn: 0.384 ± 0.013	1.042TyrPro: 1.042 ± 0.021	0.598TyrGln: 0.598 ± 0.015	1.838TyrArg: 1.838 ± 0.03	0.952TyrSer: 0.952 ± 0.019	1.15TyrThr: 1.15 ± 0.023	1.607TyrVal: 1.607 ± 0.029	0.314TyrTrp: 0.314 ± 0.011	0.42TyrTyr: 0.42 ± 0.014	0.002TyrXaa: 0.002 ± 0.001
Xaa	0.016XaaAla: 0.016 ± 0.003	0.0XaaCys: 0.0 ± 0.0	0.007XaaAsp: 0.007 ± 0.002	0.008XaaGlu: 0.008 ± 0.002	0.004XaaPhe: 0.004 ± 0.001	0.009XaaGly: 0.009 ± 0.002	0.002XaaHis: 0.002 ± 0.001	0.004XaaIle: 0.004 ± 0.001	0.003XaaLys: 0.003 ± 0.001	0.01XaaLeu: 0.01 ± 0.002	0.003XaaMet: 0.003 ± 0.001	0.001XaaAsn: 0.001 ± 0.001	0.007XaaPro: 0.007 ± 0.002	0.002XaaGln: 0.002 ± 0.001	0.007XaaArg: 0.007 ± 0.002	0.006XaaSer: 0.006 ± 0.002	0.008XaaThr: 0.008 ± 0.002	0.009XaaVal: 0.009 ± 0.002	0.003XaaTrp: 0.003 ± 0.001	0.0XaaTyr: 0.0 ± 0.0	0.005XaaXaa: 0.005 ± 0.002

Statistics based on 8062 proteins (2507143 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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