Aeromonas phage 13AhydR10PP

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S1PDD8|A0A2S1PDD8_9CAUD Putative HNH homing endonuclease OS=Aeromonas phage 13AhydR10PP OX=2163962 PE=4 SV=1
MM1 pKa = 7.58AKK3 pKa = 9.48WFAYY7 pKa = 10.53SPDD10 pKa = 3.59SGISFHH16 pKa = 6.83DD17 pKa = 3.69TPEE20 pKa = 4.25AANKK24 pKa = 7.61WAQAEE29 pKa = 4.03IDD31 pKa = 3.84DD32 pKa = 4.78CRR34 pKa = 11.84AAMDD38 pKa = 4.24DD39 pKa = 4.47LGWDD43 pKa = 3.54GDD45 pKa = 4.51LVDD48 pKa = 4.87SICWGEE54 pKa = 4.1VKK56 pKa = 10.35EE57 pKa = 4.1QAKK60 pKa = 10.47EE61 pKa = 3.97FGNCTSGFDD70 pKa = 3.72YY71 pKa = 10.8EE72 pKa = 4.71LEE74 pKa = 4.42VVV76 pKa = 3.7

Molecular weight: 8.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S1PDB5|A0A2S1PDB5_9CAUD Tail fibers protein OS=Aeromonas phage 13AhydR10PP OX=2163962 PE=4 SV=1
MM1 pKa = 6.97TRR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84TPVIATSIRR14 pKa = 11.84PPFTVHH20 pKa = 6.59HH21 pKa = 6.04FVSLKK26 pKa = 10.71DD27 pKa = 3.41AVEE30 pKa = 4.05RR31 pKa = 11.84GGFNQTSVSRR41 pKa = 11.84CLRR44 pKa = 11.84NPDD47 pKa = 3.58SVHH50 pKa = 6.69CGFKK54 pKa = 10.01FTTTVTLEE62 pKa = 3.86PLRR65 pKa = 11.84ATKK68 pKa = 10.49RR69 pKa = 11.84RR70 pKa = 11.84DD71 pKa = 3.44EE72 pKa = 4.12VHH74 pKa = 6.6ALASKK79 pKa = 10.53GYY81 pKa = 8.66SFSLIALEE89 pKa = 4.4LGIARR94 pKa = 11.84STVCYY99 pKa = 10.36HH100 pKa = 5.89MRR102 pKa = 11.84TPAA105 pKa = 3.4

Molecular weight: 11.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

80

0

80

14282

44

844

178.5

19.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.034 ± 0.463

1.47 ± 0.141

5.454 ± 0.249

6.232 ± 0.331

3.081 ± 0.146

7.814 ± 0.309

2.227 ± 0.196

4.999 ± 0.186

5.917 ± 0.287

8.486 ± 0.231

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.214 ± 0.172

3.529 ± 0.253

4.341 ± 0.266

3.704 ± 0.23

6.029 ± 0.303

5.826 ± 0.212

6.358 ± 0.279

7.121 ± 0.33

1.61 ± 0.118

2.556 ± 0.19
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski