Amino acid dipepetide frequency for Streptomyces phage Hiyaa

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.923AlaAla: 11.923 ± 1.05
1.021AlaCys: 1.021 ± 0.209
7.717AlaAsp: 7.717 ± 0.617
7.268AlaGlu: 7.268 ± 0.732
2.858AlaPhe: 2.858 ± 0.356
7.84AlaGly: 7.84 ± 0.588
1.715AlaHis: 1.715 ± 0.477
3.797AlaIle: 3.797 ± 0.406
5.471AlaLys: 5.471 ± 0.735
9.555AlaLeu: 9.555 ± 0.746
2.899AlaMet: 2.899 ± 0.3
3.471AlaAsn: 3.471 ± 0.378
4.9AlaPro: 4.9 ± 0.627
4.491AlaGln: 4.491 ± 0.463
7.35AlaArg: 7.35 ± 0.529
6.002AlaSer: 6.002 ± 0.668
6.941AlaThr: 6.941 ± 0.689
7.472AlaVal: 7.472 ± 0.544
2.613AlaTrp: 2.613 ± 0.317
2.246AlaTyr: 2.246 ± 0.319
0.0AlaXaa: 0.0 ± 0.0
Cys
0.939CysAla: 0.939 ± 0.165
0.286CysCys: 0.286 ± 0.194
0.612CysAsp: 0.612 ± 0.158
0.408CysGlu: 0.408 ± 0.146
0.286CysPhe: 0.286 ± 0.098
1.347CysGly: 1.347 ± 0.292
0.163CysHis: 0.163 ± 0.089
0.204CysIle: 0.204 ± 0.089
0.49CysLys: 0.49 ± 0.137
0.612CysLeu: 0.612 ± 0.175
0.163CysMet: 0.163 ± 0.092
0.163CysAsn: 0.163 ± 0.08
0.776CysPro: 0.776 ± 0.268
0.327CysGln: 0.327 ± 0.116
0.776CysArg: 0.776 ± 0.204
0.49CysSer: 0.49 ± 0.135
0.694CysThr: 0.694 ± 0.179
0.49CysVal: 0.49 ± 0.127
0.204CysTrp: 0.204 ± 0.082
0.286CysTyr: 0.286 ± 0.117
0.0CysXaa: 0.0 ± 0.0
Asp
8.207AspAla: 8.207 ± 0.499
0.449AspCys: 0.449 ± 0.143
4.981AspAsp: 4.981 ± 0.454
5.308AspGlu: 5.308 ± 0.578
2.287AspPhe: 2.287 ± 0.254
5.349AspGly: 5.349 ± 0.515
1.47AspHis: 1.47 ± 0.267
2.572AspIle: 2.572 ± 0.323
1.715AspLys: 1.715 ± 0.288
6.247AspLeu: 6.247 ± 0.453
1.266AspMet: 1.266 ± 0.224
1.797AspAsn: 1.797 ± 0.256
4.451AspPro: 4.451 ± 0.409
2.532AspGln: 2.532 ± 0.303
4.083AspArg: 4.083 ± 0.474
3.022AspSer: 3.022 ± 0.338
4.369AspThr: 4.369 ± 0.382
4.165AspVal: 4.165 ± 0.369
1.143AspTrp: 1.143 ± 0.222
1.266AspTyr: 1.266 ± 0.247
0.0AspXaa: 0.0 ± 0.0
Glu
6.615GluAla: 6.615 ± 0.531
0.653GluCys: 0.653 ± 0.143
3.961GluAsp: 3.961 ± 0.408
4.083GluGlu: 4.083 ± 0.516
1.919GluPhe: 1.919 ± 0.31
5.676GluGly: 5.676 ± 0.547
0.98GluHis: 0.98 ± 0.183
2.817GluIle: 2.817 ± 0.35
1.837GluLys: 1.837 ± 0.294
5.349GluLeu: 5.349 ± 0.487
1.143GluMet: 1.143 ± 0.212
2.082GluAsn: 2.082 ± 0.318
2.736GluPro: 2.736 ± 0.369
2.777GluGln: 2.777 ± 0.381
4.9GluArg: 4.9 ± 0.56
2.695GluSer: 2.695 ± 0.288
3.226GluThr: 3.226 ± 0.376
5.88GluVal: 5.88 ± 0.509
1.388GluTrp: 1.388 ± 0.234
1.674GluTyr: 1.674 ± 0.273
0.0GluXaa: 0.0 ± 0.0
Phe
2.654PheAla: 2.654 ± 0.348
0.204PheCys: 0.204 ± 0.095
2.001PheAsp: 2.001 ± 0.294
1.919PheGlu: 1.919 ± 0.283
0.653PhePhe: 0.653 ± 0.158
2.94PheGly: 2.94 ± 0.315
0.449PheHis: 0.449 ± 0.122
1.143PheIle: 1.143 ± 0.232
1.266PheLys: 1.266 ± 0.221
1.837PheLeu: 1.837 ± 0.26
0.612PheMet: 0.612 ± 0.161
1.266PheAsn: 1.266 ± 0.214
1.021PhePro: 1.021 ± 0.205
0.653PheGln: 0.653 ± 0.176
1.837PheArg: 1.837 ± 0.26
1.511PheSer: 1.511 ± 0.233
1.511PheThr: 1.511 ± 0.318
2.327PheVal: 2.327 ± 0.416
0.367PheTrp: 0.367 ± 0.153
0.817PheTyr: 0.817 ± 0.187
0.0PheXaa: 0.0 ± 0.0
Gly
6.778GlyAla: 6.778 ± 0.747
1.592GlyCys: 1.592 ± 0.318
5.104GlyAsp: 5.104 ± 0.554
4.655GlyGlu: 4.655 ± 0.445
2.899GlyPhe: 2.899 ± 0.378
7.145GlyGly: 7.145 ± 0.606
2.654GlyHis: 2.654 ± 0.395
3.511GlyIle: 3.511 ± 0.421
4.777GlyLys: 4.777 ± 0.504
5.961GlyLeu: 5.961 ± 0.55
1.347GlyMet: 1.347 ± 0.244
2.409GlyAsn: 2.409 ± 0.266
3.185GlyPro: 3.185 ± 0.533
3.552GlyGln: 3.552 ± 0.424
7.105GlyArg: 7.105 ± 0.638
5.39GlySer: 5.39 ± 0.54
6.86GlyThr: 6.86 ± 0.668
6.451GlyVal: 6.451 ± 0.51
2.042GlyTrp: 2.042 ± 0.272
2.164GlyTyr: 2.164 ± 0.292
0.0GlyXaa: 0.0 ± 0.0
His
2.082HisAla: 2.082 ± 0.36
0.122HisCys: 0.122 ± 0.078
1.429HisAsp: 1.429 ± 0.266
1.307HisGlu: 1.307 ± 0.211
0.408HisPhe: 0.408 ± 0.12
1.552HisGly: 1.552 ± 0.242
0.572HisHis: 0.572 ± 0.183
0.817HisIle: 0.817 ± 0.161
0.735HisLys: 0.735 ± 0.252
1.633HisLeu: 1.633 ± 0.295
0.612HisMet: 0.612 ± 0.158
0.327HisAsn: 0.327 ± 0.124
1.633HisPro: 1.633 ± 0.291
0.572HisGln: 0.572 ± 0.156
1.347HisArg: 1.347 ± 0.263
1.102HisSer: 1.102 ± 0.242
1.102HisThr: 1.102 ± 0.226
1.347HisVal: 1.347 ± 0.233
0.408HisTrp: 0.408 ± 0.143
0.49HisTyr: 0.49 ± 0.139
0.0HisXaa: 0.0 ± 0.0
Ile
3.552IleAla: 3.552 ± 0.456
0.327IleCys: 0.327 ± 0.148
3.511IleAsp: 3.511 ± 0.37
3.389IleGlu: 3.389 ± 0.384
0.98IlePhe: 0.98 ± 0.195
4.042IleGly: 4.042 ± 0.447
0.694IleHis: 0.694 ± 0.161
1.429IleIle: 1.429 ± 0.299
2.287IleLys: 2.287 ± 0.486
2.532IleLeu: 2.532 ± 0.285
0.653IleMet: 0.653 ± 0.169
1.878IleAsn: 1.878 ± 0.304
1.715IlePro: 1.715 ± 0.321
1.47IleGln: 1.47 ± 0.261
2.94IleArg: 2.94 ± 0.335
1.756IleSer: 1.756 ± 0.217
3.879IleThr: 3.879 ± 0.463
2.817IleVal: 2.817 ± 0.343
0.898IleTrp: 0.898 ± 0.185
0.694IleTyr: 0.694 ± 0.163
0.0IleXaa: 0.0 ± 0.0
Lys
5.39LysAla: 5.39 ± 0.572
0.286LysCys: 0.286 ± 0.122
2.409LysAsp: 2.409 ± 0.337
2.287LysGlu: 2.287 ± 0.331
1.102LysPhe: 1.102 ± 0.233
3.307LysGly: 3.307 ± 0.48
0.735LysHis: 0.735 ± 0.185
2.205LysIle: 2.205 ± 0.312
1.511LysLys: 1.511 ± 0.293
3.103LysLeu: 3.103 ± 0.423
0.98LysMet: 0.98 ± 0.24
0.857LysAsn: 0.857 ± 0.192
2.368LysPro: 2.368 ± 0.412
1.715LysGln: 1.715 ± 0.267
3.267LysArg: 3.267 ± 0.368
2.736LysSer: 2.736 ± 0.604
2.205LysThr: 2.205 ± 0.298
3.879LysVal: 3.879 ± 0.528
0.939LysTrp: 0.939 ± 0.212
0.776LysTyr: 0.776 ± 0.192
0.0LysXaa: 0.0 ± 0.0
Leu
9.269LeuAla: 9.269 ± 0.67
0.572LeuCys: 0.572 ± 0.139
5.921LeuAsp: 5.921 ± 0.488
3.756LeuGlu: 3.756 ± 0.351
1.592LeuPhe: 1.592 ± 0.317
5.921LeuGly: 5.921 ± 0.495
1.47LeuHis: 1.47 ± 0.237
3.226LeuIle: 3.226 ± 0.382
3.716LeuLys: 3.716 ± 0.465
6.043LeuLeu: 6.043 ± 0.495
2.287LeuMet: 2.287 ± 0.273
1.96LeuAsn: 1.96 ± 0.284
4.451LeuPro: 4.451 ± 0.416
2.082LeuGln: 2.082 ± 0.265
5.471LeuArg: 5.471 ± 0.429
3.756LeuSer: 3.756 ± 0.374
5.267LeuThr: 5.267 ± 0.469
6.166LeuVal: 6.166 ± 0.509
1.143LeuTrp: 1.143 ± 0.218
1.674LeuTyr: 1.674 ± 0.241
0.0LeuXaa: 0.0 ± 0.0
Met
2.858MetAla: 2.858 ± 0.315
0.204MetCys: 0.204 ± 0.093
1.225MetAsp: 1.225 ± 0.215
0.857MetGlu: 0.857 ± 0.228
0.531MetPhe: 0.531 ± 0.156
2.042MetGly: 2.042 ± 0.325
0.408MetHis: 0.408 ± 0.133
0.939MetIle: 0.939 ± 0.16
0.653MetLys: 0.653 ± 0.15
1.592MetLeu: 1.592 ± 0.29
0.531MetMet: 0.531 ± 0.144
0.857MetAsn: 0.857 ± 0.2
1.47MetPro: 1.47 ± 0.265
0.735MetGln: 0.735 ± 0.198
1.756MetArg: 1.756 ± 0.235
1.592MetSer: 1.592 ± 0.245
1.715MetThr: 1.715 ± 0.29
1.674MetVal: 1.674 ± 0.284
0.49MetTrp: 0.49 ± 0.145
0.612MetTyr: 0.612 ± 0.197
0.0MetXaa: 0.0 ± 0.0
Asn
3.185AsnAla: 3.185 ± 0.441
0.245AsnCys: 0.245 ± 0.094
2.042AsnAsp: 2.042 ± 0.279
1.429AsnGlu: 1.429 ± 0.286
0.735AsnPhe: 0.735 ± 0.148
3.552AsnGly: 3.552 ± 0.39
0.408AsnHis: 0.408 ± 0.129
1.47AsnIle: 1.47 ± 0.263
0.694AsnLys: 0.694 ± 0.165
2.817AsnLeu: 2.817 ± 0.396
0.817AsnMet: 0.817 ± 0.233
1.266AsnAsn: 1.266 ± 0.235
2.042AsnPro: 2.042 ± 0.269
1.47AsnGln: 1.47 ± 0.203
2.287AsnArg: 2.287 ± 0.325
1.756AsnSer: 1.756 ± 0.284
2.287AsnThr: 2.287 ± 0.273
2.858AsnVal: 2.858 ± 0.324
0.817AsnTrp: 0.817 ± 0.185
0.653AsnTyr: 0.653 ± 0.148
0.0AsnXaa: 0.0 ± 0.0
Pro
5.431ProAla: 5.431 ± 0.768
0.653ProCys: 0.653 ± 0.165
4.001ProAsp: 4.001 ± 0.362
3.961ProGlu: 3.961 ± 0.482
0.898ProPhe: 0.898 ± 0.207
5.839ProGly: 5.839 ± 0.662
0.939ProHis: 0.939 ± 0.193
1.878ProIle: 1.878 ± 0.259
2.246ProLys: 2.246 ± 0.374
2.899ProLeu: 2.899 ± 0.373
1.143ProMet: 1.143 ± 0.178
2.532ProAsn: 2.532 ± 0.325
2.94ProPro: 2.94 ± 0.394
1.878ProGln: 1.878 ± 0.342
2.654ProArg: 2.654 ± 0.443
2.899ProSer: 2.899 ± 0.354
3.675ProThr: 3.675 ± 0.476
3.797ProVal: 3.797 ± 0.446
1.062ProTrp: 1.062 ± 0.216
1.021ProTyr: 1.021 ± 0.212
0.0ProXaa: 0.0 ± 0.0
Gln
3.838GlnAla: 3.838 ± 0.404
0.245GlnCys: 0.245 ± 0.108
2.164GlnAsp: 2.164 ± 0.371
2.205GlnGlu: 2.205 ± 0.39
1.062GlnPhe: 1.062 ± 0.243
3.144GlnGly: 3.144 ± 0.389
1.021GlnHis: 1.021 ± 0.227
2.001GlnIle: 2.001 ± 0.387
1.266GlnLys: 1.266 ± 0.241
2.94GlnLeu: 2.94 ± 0.411
1.184GlnMet: 1.184 ± 0.207
1.062GlnAsn: 1.062 ± 0.213
2.287GlnPro: 2.287 ± 0.434
1.674GlnGln: 1.674 ± 0.268
2.572GlnArg: 2.572 ± 0.297
1.837GlnSer: 1.837 ± 0.378
2.123GlnThr: 2.123 ± 0.285
3.552GlnVal: 3.552 ± 0.352
0.49GlnTrp: 0.49 ± 0.162
0.857GlnTyr: 0.857 ± 0.242
0.0GlnXaa: 0.0 ± 0.0
Arg
7.186ArgAla: 7.186 ± 0.555
0.531ArgCys: 0.531 ± 0.16
4.165ArgAsp: 4.165 ± 0.407
4.573ArgGlu: 4.573 ± 0.548
1.756ArgPhe: 1.756 ± 0.252
4.491ArgGly: 4.491 ± 0.41
1.919ArgHis: 1.919 ± 0.32
3.348ArgIle: 3.348 ± 0.38
3.226ArgLys: 3.226 ± 0.426
5.88ArgLeu: 5.88 ± 0.586
2.001ArgMet: 2.001 ± 0.319
2.572ArgAsn: 2.572 ± 0.354
3.879ArgPro: 3.879 ± 0.569
2.777ArgGln: 2.777 ± 0.418
5.757ArgArg: 5.757 ± 0.72
3.879ArgSer: 3.879 ± 0.636
4.655ArgThr: 4.655 ± 0.408
5.471ArgVal: 5.471 ± 0.547
1.797ArgTrp: 1.797 ± 0.345
1.878ArgTyr: 1.878 ± 0.254
0.0ArgXaa: 0.0 ± 0.0
Ser
7.268SerAla: 7.268 ± 0.762
0.408SerCys: 0.408 ± 0.132
2.491SerAsp: 2.491 ± 0.299
2.94SerGlu: 2.94 ± 0.345
1.715SerPhe: 1.715 ± 0.273
6.125SerGly: 6.125 ± 0.703
1.062SerHis: 1.062 ± 0.2
2.368SerIle: 2.368 ± 0.298
2.45SerLys: 2.45 ± 0.319
3.226SerLeu: 3.226 ± 0.376
1.143SerMet: 1.143 ± 0.224
1.715SerAsn: 1.715 ± 0.255
2.123SerPro: 2.123 ± 0.244
2.368SerGln: 2.368 ± 0.426
3.185SerArg: 3.185 ± 0.383
2.817SerSer: 2.817 ± 0.361
3.471SerThr: 3.471 ± 0.425
3.43SerVal: 3.43 ± 0.385
1.184SerTrp: 1.184 ± 0.245
1.715SerTyr: 1.715 ± 0.306
0.0SerXaa: 0.0 ± 0.0
Thr
7.84ThrAla: 7.84 ± 0.961
0.694ThrCys: 0.694 ± 0.174
4.859ThrAsp: 4.859 ± 0.487
4.246ThrGlu: 4.246 ± 0.443
2.123ThrPhe: 2.123 ± 0.33
5.798ThrGly: 5.798 ± 0.487
0.857ThrHis: 0.857 ± 0.192
2.532ThrIle: 2.532 ± 0.343
2.491ThrLys: 2.491 ± 0.357
5.063ThrLeu: 5.063 ± 0.475
1.062ThrMet: 1.062 ± 0.193
2.123ThrAsn: 2.123 ± 0.315
5.104ThrPro: 5.104 ± 0.625
1.674ThrGln: 1.674 ± 0.271
4.41ThrArg: 4.41 ± 0.427
4.287ThrSer: 4.287 ± 0.654
5.186ThrThr: 5.186 ± 0.739
5.063ThrVal: 5.063 ± 0.46
1.143ThrTrp: 1.143 ± 0.223
1.878ThrTyr: 1.878 ± 0.246
0.0ThrXaa: 0.0 ± 0.0
Val
8.044ValAla: 8.044 ± 0.797
0.857ValCys: 0.857 ± 0.208
4.818ValAsp: 4.818 ± 0.456
5.104ValGlu: 5.104 ± 0.469
2.327ValPhe: 2.327 ± 0.309
5.635ValGly: 5.635 ± 0.479
1.266ValHis: 1.266 ± 0.226
3.797ValIle: 3.797 ± 0.412
3.43ValLys: 3.43 ± 0.403
4.777ValLeu: 4.777 ± 0.461
1.797ValMet: 1.797 ± 0.255
2.777ValAsn: 2.777 ± 0.319
3.716ValPro: 3.716 ± 0.435
3.348ValGln: 3.348 ± 0.377
5.553ValArg: 5.553 ± 0.486
3.552ValSer: 3.552 ± 0.408
5.921ValThr: 5.921 ± 0.54
7.023ValVal: 7.023 ± 0.616
1.919ValTrp: 1.919 ± 0.323
2.164ValTyr: 2.164 ± 0.275
0.0ValXaa: 0.0 ± 0.0
Trp
2.246TrpAla: 2.246 ± 0.283
0.204TrpCys: 0.204 ± 0.103
1.552TrpAsp: 1.552 ± 0.255
1.102TrpGlu: 1.102 ± 0.261
0.367TrpPhe: 0.367 ± 0.129
1.674TrpGly: 1.674 ± 0.271
0.408TrpHis: 0.408 ± 0.134
0.49TrpIle: 0.49 ± 0.155
0.857TrpLys: 0.857 ± 0.214
1.797TrpLeu: 1.797 ± 0.35
0.612TrpMet: 0.612 ± 0.169
0.776TrpAsn: 0.776 ± 0.253
0.653TrpPro: 0.653 ± 0.183
0.735TrpGln: 0.735 ± 0.172
1.878TrpArg: 1.878 ± 0.315
0.817TrpSer: 0.817 ± 0.173
1.715TrpThr: 1.715 ± 0.252
2.164TrpVal: 2.164 ± 0.349
0.572TrpTrp: 0.572 ± 0.156
0.735TrpTyr: 0.735 ± 0.187
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.246TyrAla: 2.246 ± 0.324
0.163TyrCys: 0.163 ± 0.068
1.715TyrAsp: 1.715 ± 0.264
1.592TyrGlu: 1.592 ± 0.29
0.49TyrPhe: 0.49 ± 0.154
2.205TyrGly: 2.205 ± 0.295
0.408TyrHis: 0.408 ± 0.116
0.898TyrIle: 0.898 ± 0.212
0.939TyrLys: 0.939 ± 0.234
1.878TyrLeu: 1.878 ± 0.294
0.449TyrMet: 0.449 ± 0.142
0.939TyrAsn: 0.939 ± 0.199
0.898TyrPro: 0.898 ± 0.203
0.694TyrGln: 0.694 ± 0.184
2.613TyrArg: 2.613 ± 0.304
1.307TyrSer: 1.307 ± 0.226
1.633TyrThr: 1.633 ± 0.256
1.797TyrVal: 1.797 ± 0.29
0.735TyrTrp: 0.735 ± 0.168
0.653TyrTyr: 0.653 ± 0.161
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 124 proteins (24492 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski