Streptococcus satellite phage Javan341
Average proteome isoelectric point is 6.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 24 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZKZ1|A0A4D5ZKZ1_9VIRU HTH cro/C1-type domain-containing protein OS=Streptococcus satellite phage Javan341 OX=2558649 GN=JavanS341_0021 PE=4 SV=1
MM1 pKa = 7.66 KK2 pKa = 10.3 IKK4 pKa = 10.74 LFYY7 pKa = 10.44 QKK9 pKa = 10.61 YY10 pKa = 8.31 KK11 pKa = 10.71 QSLEE15 pKa = 4.1 DD16 pKa = 4.85 FEE18 pKa = 5.91 SQVNDD23 pKa = 3.44 FMATVEE29 pKa = 4.3 VVDD32 pKa = 3.98 VKK34 pKa = 11.25 YY35 pKa = 11.23 SEE37 pKa = 4.27 ATVGNSDD44 pKa = 5.52 DD45 pKa = 4.23 MDD47 pKa = 4.02 TLTSVMILYY56 pKa = 9.79 KK57 pKa = 10.73
Molecular weight: 6.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.455
IPC2_protein 4.469
IPC_protein 4.279
Toseland 4.101
ProMoST 4.431
Dawson 4.253
Bjellqvist 4.418
Wikipedia 4.19
Rodwell 4.113
Grimsley 4.012
Solomon 4.24
Lehninger 4.202
Nozaki 4.38
DTASelect 4.584
Thurlkill 4.139
EMBOSS 4.19
Sillero 4.406
Patrickios 3.77
IPC_peptide 4.24
IPC2_peptide 4.38
IPC2.peptide.svr19 4.347
Protein with the highest isoelectric point:
>tr|A0A4D5ZQG3|A0A4D5ZQG3_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan341 OX=2558649 GN=JavanS341_0010 PE=4 SV=1
MM1 pKa = 7.92 KK2 pKa = 9.25 ITEE5 pKa = 4.18 YY6 pKa = 10.59 KK7 pKa = 9.86 KK8 pKa = 10.3 KK9 pKa = 10.54 NGTIVYY15 pKa = 8.89 RR16 pKa = 11.84 ASVYY20 pKa = 10.88 LGVDD24 pKa = 2.75 KK25 pKa = 10.61 LTGKK29 pKa = 10.25 KK30 pKa = 10.31 ARR32 pKa = 11.84 TTVTAKK38 pKa = 9.59 TKK40 pKa = 10.08 TGIKK44 pKa = 9.52 IKK46 pKa = 10.63 ARR48 pKa = 11.84 EE49 pKa = 4.43 AINAFANNGYY59 pKa = 8.06 SVKK62 pKa = 10.07 EE63 pKa = 4.01 KK64 pKa = 9.58 PTITTYY70 pKa = 11.1 KK71 pKa = 10.28 EE72 pKa = 4.7 LVALWWEE79 pKa = 4.57 SYY81 pKa = 11.28 KK82 pKa = 10.03 NTIKK86 pKa = 10.78 PNSQQSMEE94 pKa = 4.61 GIVRR98 pKa = 11.84 LHH100 pKa = 6.54 ILPVFGDD107 pKa = 3.7 YY108 pKa = 11.03 KK109 pKa = 10.72 LDD111 pKa = 3.64 KK112 pKa = 9.61 LTTPIIQQQVNKK124 pKa = 9.58 WADD127 pKa = 3.33 KK128 pKa = 10.63 ANKK131 pKa = 9.16 GEE133 pKa = 3.98 KK134 pKa = 9.51 GAYY137 pKa = 9.7 ANYY140 pKa = 10.84 SFLNNINRR148 pKa = 11.84 RR149 pKa = 11.84 ILQYY153 pKa = 10.72 GVTMQAIQHH162 pKa = 5.58 NPARR166 pKa = 11.84 DD167 pKa = 3.51 VIIPRR172 pKa = 11.84 KK173 pKa = 7.62 QQNKK177 pKa = 5.06 EE178 pKa = 3.84 HH179 pKa = 6.35 KK180 pKa = 10.3 VKK182 pKa = 10.6 FFSNQEE188 pKa = 3.76 LKK190 pKa = 10.87 QFLDD194 pKa = 3.59 YY195 pKa = 11.26 LDD197 pKa = 5.03 NLDD200 pKa = 3.77 LSSYY204 pKa = 10.93 EE205 pKa = 3.9 NLFDD209 pKa = 3.71 YY210 pKa = 10.96 VLYY213 pKa = 8.9 KK214 pKa = 10.39 TLLASGCRR222 pKa = 11.84 IGEE225 pKa = 4.0 ALALEE230 pKa = 4.49 WSDD233 pKa = 3.5 IDD235 pKa = 4.04 LKK237 pKa = 11.17 KK238 pKa = 11.01 GIISISKK245 pKa = 7.96 TLNRR249 pKa = 11.84 YY250 pKa = 8.7 QEE252 pKa = 4.35 TNTPKK257 pKa = 10.57 SKK259 pKa = 10.86 AGLRR263 pKa = 11.84 EE264 pKa = 3.44 IDD266 pKa = 3.3 IDD268 pKa = 3.65 KK269 pKa = 9.94 ATISLLKK276 pKa = 10.08 QYY278 pKa = 10.82 KK279 pKa = 9.6 KK280 pKa = 10.25 RR281 pKa = 11.84 QQVQSWQLGRR291 pKa = 11.84 SEE293 pKa = 5.54 GIVFTPFTTKK303 pKa = 10.34 YY304 pKa = 10.75 AYY306 pKa = 10.29 ACLLRR311 pKa = 11.84 KK312 pKa = 9.65 RR313 pKa = 11.84 LQGHH317 pKa = 6.88 LKK319 pKa = 10.21 SAGVPDD325 pKa = 3.89 ISFHH329 pKa = 5.86 GFRR332 pKa = 11.84 HH333 pKa = 4.4 THH335 pKa = 4.65 ATIMLYY341 pKa = 10.62 AGIEE345 pKa = 4.18 AKK347 pKa = 10.42 DD348 pKa = 3.35 LQYY351 pKa = 11.55 RR352 pKa = 11.84 LGHH355 pKa = 6.02 SNISMTLNTYY365 pKa = 8.7 VHH367 pKa = 6.14 ATKK370 pKa = 10.47 EE371 pKa = 4.25 GAKK374 pKa = 9.7 KK375 pKa = 10.13 AVSIFEE381 pKa = 4.24 SAISNLL387 pKa = 3.62
Molecular weight: 44.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.06
IPC2_protein 9.297
IPC_protein 9.18
Toseland 9.94
ProMoST 9.604
Dawson 10.16
Bjellqvist 9.794
Wikipedia 10.306
Rodwell 10.687
Grimsley 10.218
Solomon 10.16
Lehninger 10.131
Nozaki 9.926
DTASelect 9.794
Thurlkill 9.999
EMBOSS 10.35
Sillero 10.058
Patrickios 10.145
IPC_peptide 10.16
IPC2_peptide 8.273
IPC2.peptide.svr19 8.247
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
24
0
24
3884
39
536
161.8
18.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.334 ± 0.608
0.335 ± 0.085
6.54 ± 0.565
8.883 ± 0.79
4.222 ± 0.437
5.046 ± 0.491
1.287 ± 0.201
7.055 ± 0.611
9.604 ± 0.469
10.067 ± 0.557
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.214 ± 0.32
5.252 ± 0.346
2.111 ± 0.355
4.737 ± 0.687
5.227 ± 0.339
5.664 ± 0.401
4.866 ± 0.509
5.407 ± 0.585
0.747 ± 0.139
4.403 ± 0.381
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here