Klebsiella phage KP15

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; Slopekvirus; Klebsiella virus KP15

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 258 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E3T7Z3|E3T7Z3_9CAUD Uncharacterized protein OS=Klebsiella phage KP15 OX=707757 GN=KP15_147C PE=4 SV=1
MM1 pKa = 7.36NMYY4 pKa = 10.9EE5 pKa = 4.13MLKK8 pKa = 10.24SEE10 pKa = 4.04NSEE13 pKa = 4.17TQGEE17 pKa = 4.13KK18 pKa = 10.42DD19 pKa = 3.43YY20 pKa = 11.48NKK22 pKa = 9.34WKK24 pKa = 10.47RR25 pKa = 11.84SVQHH29 pKa = 5.8MLGSSDD35 pKa = 3.57YY36 pKa = 11.58DD37 pKa = 3.59EE38 pKa = 6.39DD39 pKa = 4.92LMYY42 pKa = 11.32ALYY45 pKa = 10.84SDD47 pKa = 4.04GCTPEE52 pKa = 5.24DD53 pKa = 4.1AVCEE57 pKa = 4.24YY58 pKa = 10.47YY59 pKa = 11.08AQGDD63 pKa = 3.91EE64 pKa = 4.32EE65 pKa = 4.3

Molecular weight: 7.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E3T7W2|E3T7W2_9CAUD Uncharacterized protein OS=Klebsiella phage KP15 OX=707757 GN=KP15_50A PE=4 SV=1
MM1 pKa = 7.16KK2 pKa = 10.17RR3 pKa = 11.84SYY5 pKa = 11.65NMNEE9 pKa = 3.59LVFFKK14 pKa = 10.23TRR16 pKa = 11.84RR17 pKa = 11.84EE18 pKa = 3.6ARR20 pKa = 11.84AYY22 pKa = 10.51VLAAGKK28 pKa = 7.95NTSAAIDD35 pKa = 3.7MGTDD39 pKa = 3.15KK40 pKa = 11.32AVGARR45 pKa = 11.84WAAVIVIAAPVTPVKK60 pKa = 10.13TLVLGARR67 pKa = 11.84HH68 pKa = 6.69AEE70 pKa = 4.22TLNTAASGRR79 pKa = 11.84GHH81 pKa = 5.46EE82 pKa = 4.17VKK84 pKa = 10.57VFKK87 pKa = 10.58KK88 pKa = 10.43RR89 pKa = 11.84SYY91 pKa = 10.47LSCARR96 pKa = 11.84GG97 pKa = 3.1

Molecular weight: 10.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

257

1

258

54704

46

1328

212.0

24.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.654 ± 0.177

1.19 ± 0.07

6.601 ± 0.09

6.91 ± 0.18

4.131 ± 0.112

6.281 ± 0.245

1.759 ± 0.081

6.815 ± 0.127

7.292 ± 0.194

7.51 ± 0.131

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.967 ± 0.088

5.513 ± 0.119

3.528 ± 0.097

3.289 ± 0.098

4.802 ± 0.112

6.096 ± 0.152

5.958 ± 0.211

6.99 ± 0.121

1.302 ± 0.052

4.413 ± 0.117
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski