Firmicutes bacterium CAG:313

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1321 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6YVQ3|R6YVQ3_9FIRM Pyridine nucleotide-disulfide oxidoreductase OS=Firmicutes bacterium CAG:313 OX=1263017 GN=BN602_01305 PE=4 SV=1
MM1 pKa = 7.65FDD3 pKa = 3.69SDD5 pKa = 4.9SNTEE9 pKa = 3.73RR10 pKa = 11.84YY11 pKa = 9.69LFYY14 pKa = 11.04KK15 pKa = 10.38LCNKK19 pKa = 10.19LYY21 pKa = 9.21YY22 pKa = 10.17DD23 pKa = 3.8YY24 pKa = 11.85VSIEE28 pKa = 4.25DD29 pKa = 3.76TDD31 pKa = 3.9IDD33 pKa = 3.93DD34 pKa = 5.64LEE36 pKa = 5.1DD37 pKa = 3.46VLYY40 pKa = 11.02DD41 pKa = 3.81EE42 pKa = 6.16LDD44 pKa = 2.86WSYY47 pKa = 11.4PFDD50 pKa = 4.66IEE52 pKa = 4.35PTDD55 pKa = 4.9DD56 pKa = 3.39EE57 pKa = 4.37CQKK60 pKa = 10.73FKK62 pKa = 11.09EE63 pKa = 3.97ALEE66 pKa = 4.33YY67 pKa = 10.98YY68 pKa = 10.03ISSKK72 pKa = 8.68QKK74 pKa = 10.85KK75 pKa = 8.64NVV77 pKa = 3.02

Molecular weight: 9.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6XQM4|R6XQM4_9FIRM Alpha/beta hydrolase fold OS=Firmicutes bacterium CAG:313 OX=1263017 GN=BN602_00019 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.24QPNKK9 pKa = 8.37RR10 pKa = 11.84KK11 pKa = 9.68HH12 pKa = 6.01SKK14 pKa = 7.21THH16 pKa = 5.41GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 8.95VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.81GRR39 pKa = 11.84AVLSAA44 pKa = 3.96

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1321

0

1321

443435

29

4235

335.7

38.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.654 ± 0.075

1.239 ± 0.027

5.582 ± 0.052

7.068 ± 0.072

4.374 ± 0.046

5.749 ± 0.067

1.469 ± 0.027

9.527 ± 0.092

8.785 ± 0.066

9.037 ± 0.087

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.355 ± 0.03

6.618 ± 0.058

2.728 ± 0.033

2.539 ± 0.029

3.002 ± 0.048

5.932 ± 0.053

5.87 ± 0.079

6.814 ± 0.057

0.647 ± 0.021

5.014 ± 0.061
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski