Amino acid dipepetide frequency for Flavobacterium sp. phage 1/32

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.0AlaAla: 0.0 ± 0.0
0.219AlaCys: 0.219 ± 0.129
2.632AlaAsp: 2.632 ± 0.412
5.703AlaGlu: 5.703 ± 1.066
3.29AlaPhe: 3.29 ± 0.506
3.729AlaGly: 3.729 ± 0.587
0.951AlaHis: 0.951 ± 0.295
4.46AlaIle: 4.46 ± 0.621
6.435AlaLys: 6.435 ± 1.049
5.923AlaLeu: 5.923 ± 0.913
1.316AlaMet: 1.316 ± 0.405
3.364AlaAsn: 3.364 ± 0.513
1.316AlaPro: 1.316 ± 0.295
2.267AlaGln: 2.267 ± 0.421
1.536AlaArg: 1.536 ± 0.361
2.559AlaSer: 2.559 ± 0.388
3.217AlaThr: 3.217 ± 0.49
3.729AlaVal: 3.729 ± 0.549
0.585AlaTrp: 0.585 ± 0.206
2.047AlaTyr: 2.047 ± 0.365
0.0AlaXaa: 0.0 ± 0.0
Cys
0.292CysAla: 0.292 ± 0.137
0.146CysCys: 0.146 ± 0.091
0.512CysAsp: 0.512 ± 0.234
1.17CysGlu: 1.17 ± 0.363
0.585CysPhe: 0.585 ± 0.211
1.024CysGly: 1.024 ± 0.324
0.146CysHis: 0.146 ± 0.112
0.585CysIle: 0.585 ± 0.209
0.439CysLys: 0.439 ± 0.17
0.439CysLeu: 0.439 ± 0.183
0.146CysMet: 0.146 ± 0.098
0.292CysAsn: 0.292 ± 0.132
0.585CysPro: 0.585 ± 0.231
0.292CysGln: 0.292 ± 0.141
0.0CysArg: 0.0 ± 0.0
0.512CysSer: 0.512 ± 0.21
0.658CysThr: 0.658 ± 0.277
0.292CysVal: 0.292 ± 0.16
0.073CysTrp: 0.073 ± 0.076
0.439CysTyr: 0.439 ± 0.173
0.0CysXaa: 0.0 ± 0.0
Asp
2.852AspAla: 2.852 ± 0.458
0.512AspCys: 0.512 ± 0.183
2.559AspAsp: 2.559 ± 0.378
3.51AspGlu: 3.51 ± 0.507
4.241AspPhe: 4.241 ± 0.485
3.802AspGly: 3.802 ± 0.536
0.439AspHis: 0.439 ± 0.152
2.632AspIle: 2.632 ± 0.484
4.387AspLys: 4.387 ± 0.561
6.435AspLeu: 6.435 ± 0.805
0.731AspMet: 0.731 ± 0.224
2.925AspAsn: 2.925 ± 0.549
1.974AspPro: 1.974 ± 0.319
1.389AspGln: 1.389 ± 0.362
1.974AspArg: 1.974 ± 0.432
1.974AspSer: 1.974 ± 0.396
2.267AspThr: 2.267 ± 0.475
2.632AspVal: 2.632 ± 0.546
1.536AspTrp: 1.536 ± 0.377
2.705AspTyr: 2.705 ± 0.589
0.0AspXaa: 0.0 ± 0.0
Glu
7.531GluAla: 7.531 ± 1.121
0.951GluCys: 0.951 ± 0.302
3.583GluAsp: 3.583 ± 0.448
12.211GluGlu: 12.211 ± 1.317
3.875GluPhe: 3.875 ± 0.513
3.729GluGly: 3.729 ± 0.616
1.097GluHis: 1.097 ± 0.311
8.116GluIle: 8.116 ± 0.682
10.968GluLys: 10.968 ± 1.386
9.286GluLeu: 9.286 ± 1.169
2.121GluMet: 2.121 ± 0.456
5.777GluAsn: 5.777 ± 0.789
1.536GluPro: 1.536 ± 0.386
3.729GluGln: 3.729 ± 0.556
4.241GluArg: 4.241 ± 0.538
3.583GluSer: 3.583 ± 0.529
5.118GluThr: 5.118 ± 0.694
6.8GluVal: 6.8 ± 0.769
0.951GluTrp: 0.951 ± 0.27
3.071GluTyr: 3.071 ± 0.406
0.0GluXaa: 0.0 ± 0.0
Phe
2.121PheAla: 2.121 ± 0.355
0.731PheCys: 0.731 ± 0.243
3.071PheAsp: 3.071 ± 0.507
3.949PheGlu: 3.949 ± 0.574
2.194PhePhe: 2.194 ± 0.39
2.486PheGly: 2.486 ± 0.377
0.731PheHis: 0.731 ± 0.218
4.095PheIle: 4.095 ± 0.788
5.338PheLys: 5.338 ± 0.536
3.802PheLeu: 3.802 ± 0.446
1.17PheMet: 1.17 ± 0.284
3.51PheAsn: 3.51 ± 0.621
1.536PhePro: 1.536 ± 0.401
1.609PheGln: 1.609 ± 0.277
1.828PheArg: 1.828 ± 0.351
3.437PheSer: 3.437 ± 0.487
2.779PheThr: 2.779 ± 0.463
2.705PheVal: 2.705 ± 0.537
0.804PheTrp: 0.804 ± 0.252
1.901PheTyr: 1.901 ± 0.436
0.0PheXaa: 0.0 ± 0.0
Gly
3.437GlyAla: 3.437 ± 0.528
0.439GlyCys: 0.439 ± 0.172
2.559GlyAsp: 2.559 ± 0.483
4.241GlyGlu: 4.241 ± 0.46
3.729GlyPhe: 3.729 ± 0.529
2.852GlyGly: 2.852 ± 0.649
0.512GlyHis: 0.512 ± 0.229
4.241GlyIle: 4.241 ± 0.529
5.557GlyLys: 5.557 ± 0.72
5.923GlyLeu: 5.923 ± 0.545
1.462GlyMet: 1.462 ± 0.255
3.437GlyAsn: 3.437 ± 0.543
0.0GlyPro: 0.0 ± 0.0
1.609GlyGln: 1.609 ± 0.347
2.559GlyArg: 2.559 ± 0.331
2.047GlySer: 2.047 ± 0.377
3.656GlyThr: 3.656 ± 0.483
4.826GlyVal: 4.826 ± 0.643
1.243GlyTrp: 1.243 ± 0.395
1.828GlyTyr: 1.828 ± 0.39
0.0GlyXaa: 0.0 ± 0.0
His
0.366HisAla: 0.366 ± 0.193
0.219HisCys: 0.219 ± 0.139
0.658HisAsp: 0.658 ± 0.205
0.731HisGlu: 0.731 ± 0.211
0.804HisPhe: 0.804 ± 0.216
0.512HisGly: 0.512 ± 0.197
0.146HisHis: 0.146 ± 0.112
0.512HisIle: 0.512 ± 0.212
1.243HisLys: 1.243 ± 0.26
1.389HisLeu: 1.389 ± 0.383
0.073HisMet: 0.073 ± 0.075
0.951HisAsn: 0.951 ± 0.389
0.512HisPro: 0.512 ± 0.242
0.292HisGln: 0.292 ± 0.145
0.585HisArg: 0.585 ± 0.239
0.512HisSer: 0.512 ± 0.223
0.366HisThr: 0.366 ± 0.136
0.658HisVal: 0.658 ± 0.247
0.366HisTrp: 0.366 ± 0.139
0.658HisTyr: 0.658 ± 0.218
0.0HisXaa: 0.0 ± 0.0
Ile
3.729IleAla: 3.729 ± 0.559
0.731IleCys: 0.731 ± 0.267
4.972IleAsp: 4.972 ± 0.616
8.19IleGlu: 8.19 ± 0.843
2.705IlePhe: 2.705 ± 0.394
4.46IleGly: 4.46 ± 0.49
0.658IleHis: 0.658 ± 0.235
6.142IleIle: 6.142 ± 0.59
9.506IleLys: 9.506 ± 0.964
6.288IleLeu: 6.288 ± 0.636
1.097IleMet: 1.097 ± 0.297
4.753IleAsn: 4.753 ± 0.55
2.486IlePro: 2.486 ± 0.338
2.121IleGln: 2.121 ± 0.51
2.194IleArg: 2.194 ± 0.415
3.29IleSer: 3.29 ± 0.469
5.192IleThr: 5.192 ± 0.532
3.729IleVal: 3.729 ± 0.507
0.804IleTrp: 0.804 ± 0.265
2.121IleTyr: 2.121 ± 0.414
0.0IleXaa: 0.0 ± 0.0
Lys
7.97LysAla: 7.97 ± 1.249
0.658LysCys: 0.658 ± 0.233
4.972LysAsp: 4.972 ± 0.664
11.846LysGlu: 11.846 ± 1.139
3.51LysPhe: 3.51 ± 0.499
5.63LysGly: 5.63 ± 0.718
1.389LysHis: 1.389 ± 0.326
7.751LysIle: 7.751 ± 0.654
12.211LysLys: 12.211 ± 1.136
7.897LysLeu: 7.897 ± 0.719
2.121LysMet: 2.121 ± 0.422
7.239LysAsn: 7.239 ± 0.626
4.022LysPro: 4.022 ± 0.562
3.656LysGln: 3.656 ± 0.567
5.192LysArg: 5.192 ± 0.651
4.533LysSer: 4.533 ± 0.819
6.581LysThr: 6.581 ± 0.879
5.045LysVal: 5.045 ± 0.491
0.585LysTrp: 0.585 ± 0.223
4.168LysTyr: 4.168 ± 0.51
0.0LysXaa: 0.0 ± 0.0
Leu
5.411LeuAla: 5.411 ± 0.758
0.366LeuCys: 0.366 ± 0.171
5.411LeuAsp: 5.411 ± 0.568
10.31LeuGlu: 10.31 ± 1.269
4.899LeuPhe: 4.899 ± 0.632
5.63LeuGly: 5.63 ± 0.645
0.804LeuHis: 0.804 ± 0.253
7.166LeuIle: 7.166 ± 0.662
10.456LeuLys: 10.456 ± 1.034
6.508LeuLeu: 6.508 ± 0.853
1.901LeuMet: 1.901 ± 0.358
6.581LeuAsn: 6.581 ± 0.688
2.34LeuPro: 2.34 ± 0.465
4.095LeuGln: 4.095 ± 0.622
3.583LeuArg: 3.583 ± 0.441
4.753LeuSer: 4.753 ± 0.593
6.069LeuThr: 6.069 ± 0.664
5.045LeuVal: 5.045 ± 0.579
0.439LeuTrp: 0.439 ± 0.154
1.682LeuTyr: 1.682 ± 0.352
0.0LeuXaa: 0.0 ± 0.0
Met
1.243MetAla: 1.243 ± 0.301
0.073MetCys: 0.073 ± 0.07
1.17MetAsp: 1.17 ± 0.267
2.267MetGlu: 2.267 ± 0.376
0.658MetPhe: 0.658 ± 0.288
0.658MetGly: 0.658 ± 0.258
0.366MetHis: 0.366 ± 0.186
1.097MetIle: 1.097 ± 0.295
1.609MetLys: 1.609 ± 0.399
1.682MetLeu: 1.682 ± 0.349
0.219MetMet: 0.219 ± 0.115
1.682MetAsn: 1.682 ± 0.332
0.658MetPro: 0.658 ± 0.204
0.951MetGln: 0.951 ± 0.223
0.292MetArg: 0.292 ± 0.185
0.512MetSer: 0.512 ± 0.166
1.17MetThr: 1.17 ± 0.352
0.951MetVal: 0.951 ± 0.344
0.073MetTrp: 0.073 ± 0.059
0.585MetTyr: 0.585 ± 0.192
0.0MetXaa: 0.0 ± 0.0
Asn
4.022AsnAla: 4.022 ± 0.564
0.731AsnCys: 0.731 ± 0.218
3.217AsnAsp: 3.217 ± 0.553
5.777AsnGlu: 5.777 ± 0.686
2.779AsnPhe: 2.779 ± 0.442
4.899AsnGly: 4.899 ± 0.691
0.658AsnHis: 0.658 ± 0.257
3.949AsnIle: 3.949 ± 0.473
5.777AsnLys: 5.777 ± 0.581
7.02AsnLeu: 7.02 ± 0.623
1.097AsnMet: 1.097 ± 0.309
2.998AsnAsn: 2.998 ± 0.81
2.413AsnPro: 2.413 ± 0.399
2.194AsnGln: 2.194 ± 0.476
2.559AsnArg: 2.559 ± 0.407
3.217AsnSer: 3.217 ± 0.538
4.241AsnThr: 4.241 ± 0.606
3.217AsnVal: 3.217 ± 0.578
0.658AsnTrp: 0.658 ± 0.238
3.071AsnTyr: 3.071 ± 0.483
0.0AsnXaa: 0.0 ± 0.0
Pro
0.804ProAla: 0.804 ± 0.259
0.512ProCys: 0.512 ± 0.197
1.17ProAsp: 1.17 ± 0.265
1.974ProGlu: 1.974 ± 0.396
1.974ProPhe: 1.974 ± 0.398
0.0ProGly: 0.0 ± 0.0
0.366ProHis: 0.366 ± 0.166
3.144ProIle: 3.144 ± 0.362
3.437ProLys: 3.437 ± 0.604
2.34ProLeu: 2.34 ± 0.377
0.512ProMet: 0.512 ± 0.237
2.779ProAsn: 2.779 ± 0.474
0.366ProPro: 0.366 ± 0.186
0.877ProGln: 0.877 ± 0.237
0.951ProArg: 0.951 ± 0.279
1.389ProSer: 1.389 ± 0.365
2.413ProThr: 2.413 ± 0.474
1.536ProVal: 1.536 ± 0.291
0.0ProTrp: 0.0 ± 0.0
1.316ProTyr: 1.316 ± 0.289
0.0ProXaa: 0.0 ± 0.0
Gln
2.194GlnAla: 2.194 ± 0.59
0.366GlnCys: 0.366 ± 0.193
2.194GlnAsp: 2.194 ± 0.418
3.364GlnGlu: 3.364 ± 0.507
1.024GlnPhe: 1.024 ± 0.346
1.609GlnGly: 1.609 ± 0.298
0.146GlnHis: 0.146 ± 0.11
2.925GlnIle: 2.925 ± 0.437
3.29GlnLys: 3.29 ± 0.571
3.437GlnLeu: 3.437 ± 0.674
0.951GlnMet: 0.951 ± 0.34
1.755GlnAsn: 1.755 ± 0.364
0.877GlnPro: 0.877 ± 0.195
1.024GlnGln: 1.024 ± 0.267
1.901GlnArg: 1.901 ± 0.367
2.267GlnSer: 2.267 ± 0.373
2.34GlnThr: 2.34 ± 0.428
2.267GlnVal: 2.267 ± 0.387
0.366GlnTrp: 0.366 ± 0.172
1.097GlnTyr: 1.097 ± 0.276
0.0GlnXaa: 0.0 ± 0.0
Arg
2.194ArgAla: 2.194 ± 0.457
0.366ArgCys: 0.366 ± 0.159
1.389ArgAsp: 1.389 ± 0.284
2.998ArgGlu: 2.998 ± 0.482
2.34ArgPhe: 2.34 ± 0.502
2.047ArgGly: 2.047 ± 0.375
0.439ArgHis: 0.439 ± 0.188
3.437ArgIle: 3.437 ± 0.414
3.949ArgLys: 3.949 ± 0.566
4.022ArgLeu: 4.022 ± 0.49
0.731ArgMet: 0.731 ± 0.25
2.194ArgAsn: 2.194 ± 0.366
0.585ArgPro: 0.585 ± 0.193
1.462ArgGln: 1.462 ± 0.312
1.682ArgArg: 1.682 ± 0.363
1.974ArgSer: 1.974 ± 0.418
1.828ArgThr: 1.828 ± 0.429
2.194ArgVal: 2.194 ± 0.35
0.366ArgTrp: 0.366 ± 0.157
1.243ArgTyr: 1.243 ± 0.31
0.0ArgXaa: 0.0 ± 0.0
Ser
1.609SerAla: 1.609 ± 0.35
0.439SerCys: 0.439 ± 0.168
2.486SerAsp: 2.486 ± 0.38
5.045SerGlu: 5.045 ± 0.512
2.925SerPhe: 2.925 ± 0.381
2.705SerGly: 2.705 ± 0.448
0.585SerHis: 0.585 ± 0.174
4.168SerIle: 4.168 ± 0.484
4.168SerLys: 4.168 ± 0.672
4.022SerLeu: 4.022 ± 0.572
0.585SerMet: 0.585 ± 0.155
2.705SerAsn: 2.705 ± 0.367
1.462SerPro: 1.462 ± 0.322
1.609SerGln: 1.609 ± 0.386
1.389SerArg: 1.389 ± 0.271
2.194SerSer: 2.194 ± 0.382
1.828SerThr: 1.828 ± 0.397
2.852SerVal: 2.852 ± 0.495
0.512SerTrp: 0.512 ± 0.191
2.121SerTyr: 2.121 ± 0.35
0.0SerXaa: 0.0 ± 0.0
Thr
3.656ThrAla: 3.656 ± 0.518
0.292ThrCys: 0.292 ± 0.169
3.144ThrAsp: 3.144 ± 0.4
5.703ThrGlu: 5.703 ± 0.822
2.925ThrPhe: 2.925 ± 0.537
3.583ThrGly: 3.583 ± 0.429
0.731ThrHis: 0.731 ± 0.228
4.095ThrIle: 4.095 ± 0.699
6.288ThrLys: 6.288 ± 0.777
5.85ThrLeu: 5.85 ± 0.707
0.439ThrMet: 0.439 ± 0.208
4.826ThrAsn: 4.826 ± 0.731
2.559ThrPro: 2.559 ± 0.384
2.852ThrGln: 2.852 ± 0.474
1.389ThrArg: 1.389 ± 0.29
1.974ThrSer: 1.974 ± 0.356
3.875ThrThr: 3.875 ± 0.489
3.51ThrVal: 3.51 ± 0.429
0.292ThrTrp: 0.292 ± 0.148
1.828ThrTyr: 1.828 ± 0.49
0.0ThrXaa: 0.0 ± 0.0
Val
3.51ValAla: 3.51 ± 0.407
0.439ValCys: 0.439 ± 0.189
2.486ValAsp: 2.486 ± 0.475
4.899ValGlu: 4.899 ± 0.563
2.779ValPhe: 2.779 ± 0.546
3.875ValGly: 3.875 ± 0.49
0.731ValHis: 0.731 ± 0.24
4.095ValIle: 4.095 ± 0.598
7.166ValLys: 7.166 ± 0.776
6.142ValLeu: 6.142 ± 0.668
0.951ValMet: 0.951 ± 0.345
3.364ValAsn: 3.364 ± 0.447
1.609ValPro: 1.609 ± 0.336
1.682ValGln: 1.682 ± 0.322
1.901ValArg: 1.901 ± 0.324
2.559ValSer: 2.559 ± 0.421
3.217ValThr: 3.217 ± 0.561
3.729ValVal: 3.729 ± 0.603
0.512ValTrp: 0.512 ± 0.206
2.486ValTyr: 2.486 ± 0.404
0.0ValXaa: 0.0 ± 0.0
Trp
0.292TrpAla: 0.292 ± 0.134
0.073TrpCys: 0.073 ± 0.066
0.658TrpAsp: 0.658 ± 0.216
1.097TrpGlu: 1.097 ± 0.221
0.366TrpPhe: 0.366 ± 0.148
0.951TrpGly: 0.951 ± 0.258
0.219TrpHis: 0.219 ± 0.118
0.439TrpIle: 0.439 ± 0.17
0.877TrpLys: 0.877 ± 0.306
1.609TrpLeu: 1.609 ± 0.375
0.0TrpMet: 0.0 ± 0.0
0.877TrpAsn: 0.877 ± 0.344
0.0TrpPro: 0.0 ± 0.0
0.585TrpGln: 0.585 ± 0.228
0.146TrpArg: 0.146 ± 0.111
0.439TrpSer: 0.439 ± 0.149
0.439TrpThr: 0.439 ± 0.165
0.951TrpVal: 0.951 ± 0.257
0.073TrpTrp: 0.073 ± 0.081
0.512TrpTyr: 0.512 ± 0.245
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.047TyrAla: 2.047 ± 0.422
0.366TyrCys: 0.366 ± 0.178
2.632TyrAsp: 2.632 ± 0.505
3.29TyrGlu: 3.29 ± 0.478
2.194TyrPhe: 2.194 ± 0.499
1.974TyrGly: 1.974 ± 0.388
0.439TyrHis: 0.439 ± 0.163
2.121TyrIle: 2.121 ± 0.414
3.949TyrLys: 3.949 ± 0.668
3.437TyrLeu: 3.437 ± 0.638
0.219TyrMet: 0.219 ± 0.159
2.486TyrAsn: 2.486 ± 0.455
1.024TyrPro: 1.024 ± 0.223
1.097TyrGln: 1.097 ± 0.26
1.536TyrArg: 1.536 ± 0.362
1.682TyrSer: 1.682 ± 0.396
2.559TyrThr: 2.559 ± 0.517
1.462TyrVal: 1.462 ± 0.357
0.366TyrTrp: 0.366 ± 0.169
1.316TyrTyr: 1.316 ± 0.312
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 63 proteins (13677 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski