Phodopus sungorus papillomavirus 1
Average proteome isoelectric point is 6.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W8ZM63|W8ZM63_9PAPI Minor capsid protein L2 OS=Phodopus sungorus papillomavirus 1 OX=1487796 GN=L2 PE=3 SV=1
MM1 pKa = 6.93 VAVNRR6 pKa = 11.84 ARR8 pKa = 11.84 RR9 pKa = 11.84 TKK11 pKa = 10.33 RR12 pKa = 11.84 DD13 pKa = 3.37 SASNLYY19 pKa = 9.65 RR20 pKa = 11.84 QCQVTGNCPPDD31 pKa = 3.52 VVNKK35 pKa = 10.44 IEE37 pKa = 4.09 GTTLADD43 pKa = 3.4 KK44 pKa = 10.55 LSKK47 pKa = 10.35 IFASILYY54 pKa = 10.19 LGGLGIGTGRR64 pKa = 11.84 GSGGATGYY72 pKa = 11.27 GPINPGGGRR81 pKa = 11.84 ITGTGTVMRR90 pKa = 11.84 PGVVIDD96 pKa = 3.83 PVGPNDD102 pKa = 4.26 IITVGATDD110 pKa = 3.61 SSIVPLLEE118 pKa = 4.09 ATPDD122 pKa = 3.28 IPIEE126 pKa = 4.48 GGPEE130 pKa = 3.79 VPPAGPDD137 pKa = 3.19 VSTVDD142 pKa = 3.03 VTANVDD148 pKa = 3.94 PISEE152 pKa = 4.45 VNVTTGSTITNPDD165 pKa = 3.21 SAVIDD170 pKa = 3.93 VQPAPSGPRR179 pKa = 11.84 RR180 pKa = 11.84 VTVSRR185 pKa = 11.84 SEE187 pKa = 4.07 FQNASYY193 pKa = 11.39 VSVTHH198 pKa = 6.8 PSQGLGEE205 pKa = 4.28 SGGALVSADD214 pKa = 3.26 SSGSFVSSGHH224 pKa = 5.83 EE225 pKa = 3.76 LDD227 pKa = 3.11 IGIIIGEE234 pKa = 4.26 RR235 pKa = 11.84 PLGEE239 pKa = 4.35 TNFEE243 pKa = 4.38 AIEE246 pKa = 4.15 LDD248 pKa = 4.01 EE249 pKa = 4.52 ILTTGGTAEE258 pKa = 3.92 FDD260 pKa = 2.87 IGEE263 pKa = 4.4 GVNRR267 pKa = 11.84 VGPSTSTPDD276 pKa = 3.27 NYY278 pKa = 10.8 LGRR281 pKa = 11.84 ALSRR285 pKa = 11.84 FRR287 pKa = 11.84 EE288 pKa = 4.33 IYY290 pKa = 10.21 DD291 pKa = 3.79 RR292 pKa = 11.84 PRR294 pKa = 11.84 DD295 pKa = 3.53 LYY297 pKa = 10.87 NRR299 pKa = 11.84 RR300 pKa = 11.84 VQQVRR305 pKa = 11.84 VRR307 pKa = 11.84 NRR309 pKa = 11.84 DD310 pKa = 3.14 QFLGAPGRR318 pKa = 11.84 LVTYY322 pKa = 10.05 EE323 pKa = 3.83 FDD325 pKa = 3.55 NPAFAGVEE333 pKa = 4.2 DD334 pKa = 4.49 EE335 pKa = 4.52 VSLIFQQDD343 pKa = 3.57 LNEE346 pKa = 4.2 VQAAPDD352 pKa = 3.94 TDD354 pKa = 3.78 FMDD357 pKa = 5.11 IIRR360 pKa = 11.84 LGRR363 pKa = 11.84 EE364 pKa = 4.31 RR365 pKa = 11.84 IAEE368 pKa = 4.21 TADD371 pKa = 3.05 GTVRR375 pKa = 11.84 ISRR378 pKa = 11.84 LGQRR382 pKa = 11.84 GTIRR386 pKa = 11.84 TRR388 pKa = 11.84 SGLQIGGKK396 pKa = 6.93 VHH398 pKa = 7.5 FYY400 pKa = 10.96 TDD402 pKa = 4.01 LSPIATEE409 pKa = 4.24 NIEE412 pKa = 4.3 LSTLGEE418 pKa = 4.11 VSGEE422 pKa = 4.14 SMTIDD427 pKa = 3.39 ALNEE431 pKa = 3.57 YY432 pKa = 10.67 SLISEE437 pKa = 4.41 SGIEE441 pKa = 4.13 PVPFPDD447 pKa = 3.98 EE448 pKa = 4.79 DD449 pKa = 3.96 LLDD452 pKa = 3.61 VHH454 pKa = 8.26 SEE456 pKa = 4.04 DD457 pKa = 4.2 FSGSRR462 pKa = 11.84 LHH464 pKa = 6.85 IFRR467 pKa = 11.84 GGNTRR472 pKa = 11.84 FGVVYY477 pKa = 9.91 EE478 pKa = 4.19 PSEE481 pKa = 4.18 SLGARR486 pKa = 11.84 TIFPDD491 pKa = 3.08 IDD493 pKa = 3.2 TGDD496 pKa = 3.8 FVRR499 pKa = 11.84 YY500 pKa = 7.93 PQTNVSPADD509 pKa = 3.26 IPGRR513 pKa = 11.84 DD514 pKa = 4.29 LVPVDD519 pKa = 4.27 PGTTHH524 pKa = 6.67 IVGSEE529 pKa = 4.04 FSSVDD534 pKa = 3.67 YY535 pKa = 10.65 YY536 pKa = 11.16 LHH538 pKa = 6.68 PSLRR542 pKa = 11.84 RR543 pKa = 11.84 RR544 pKa = 11.84 KK545 pKa = 9.57 RR546 pKa = 11.84 KK547 pKa = 10.03 RR548 pKa = 11.84 NFHH551 pKa = 6.21
Molecular weight: 59.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.789
IPC2_protein 4.736
IPC_protein 4.698
Toseland 4.546
ProMoST 4.8
Dawson 4.66
Bjellqvist 4.8
Wikipedia 4.533
Rodwell 4.546
Grimsley 4.457
Solomon 4.66
Lehninger 4.609
Nozaki 4.762
DTASelect 4.952
Thurlkill 4.546
EMBOSS 4.558
Sillero 4.825
Patrickios 4.139
IPC_peptide 4.66
IPC2_peptide 4.813
IPC2.peptide.svr19 4.788
Protein with the highest isoelectric point:
>tr|A0A078BR99|A0A078BR99_9PAPI Protein E7 OS=Phodopus sungorus papillomavirus 1 OX=1487796 GN=E7 PE=3 SV=1
MM1 pKa = 7.24 PTLTEE6 pKa = 4.35 RR7 pKa = 11.84 FDD9 pKa = 3.95 AVQDD13 pKa = 3.86 QILSLIEE20 pKa = 4.24 RR21 pKa = 11.84 GSTRR25 pKa = 11.84 LADD28 pKa = 3.54 HH29 pKa = 5.81 VVYY32 pKa = 10.22 WDD34 pKa = 3.66 LVRR37 pKa = 11.84 KK38 pKa = 9.95 EE39 pKa = 4.86 GVLQYY44 pKa = 9.69 YY45 pKa = 10.49 ARR47 pKa = 11.84 RR48 pKa = 11.84 QNMTRR53 pKa = 11.84 LGLLPLPSQLGAEE66 pKa = 4.56 HH67 pKa = 7.04 KK68 pKa = 10.33 AKK70 pKa = 10.5 KK71 pKa = 9.45 AIQMGLLLRR80 pKa = 11.84 SLAEE84 pKa = 3.93 SPFANEE90 pKa = 3.43 TWTVTDD96 pKa = 3.4 TSVEE100 pKa = 4.31 VYY102 pKa = 10.14 EE103 pKa = 4.13 QTDD106 pKa = 3.54 PEE108 pKa = 4.4 KK109 pKa = 10.22 TFKK112 pKa = 11.1 KK113 pKa = 9.98 NGQTIEE119 pKa = 3.73 VWYY122 pKa = 10.42 DD123 pKa = 3.19 NDD125 pKa = 4.01 PEE127 pKa = 4.25 NSVAYY132 pKa = 7.8 TLWRR136 pKa = 11.84 YY137 pKa = 9.14 IYY139 pKa = 10.41 KK140 pKa = 9.86 QDD142 pKa = 5.4 EE143 pKa = 3.87 NGQWHH148 pKa = 6.59 KK149 pKa = 11.53 LEE151 pKa = 4.26 GRR153 pKa = 11.84 VDD155 pKa = 3.72 YY156 pKa = 11.32 AGLYY160 pKa = 10.07 YY161 pKa = 9.72 IDD163 pKa = 4.19 PSGMRR168 pKa = 11.84 VYY170 pKa = 11.15 YY171 pKa = 10.55 EE172 pKa = 5.36 DD173 pKa = 4.02 FFEE176 pKa = 5.03 DD177 pKa = 3.46 ADD179 pKa = 4.53 RR180 pKa = 11.84 YY181 pKa = 9.45 GHH183 pKa = 6.32 SSTWTVKK190 pKa = 10.18 GDD192 pKa = 3.51 KK193 pKa = 10.39 DD194 pKa = 3.02 ISAPVTSSDD203 pKa = 3.47 KK204 pKa = 10.44 SWRR207 pKa = 11.84 AQEE210 pKa = 4.18 EE211 pKa = 4.22 QPKK214 pKa = 8.78 TKK216 pKa = 9.98 RR217 pKa = 11.84 RR218 pKa = 11.84 RR219 pKa = 11.84 DD220 pKa = 3.47 NPTTSTPEE228 pKa = 3.14 WDD230 pKa = 3.72 YY231 pKa = 11.67 PEE233 pKa = 4.53 TSEE236 pKa = 5.17 QEE238 pKa = 4.06 EE239 pKa = 4.5 RR240 pKa = 11.84 PQTSTDD246 pKa = 3.05 TPRR249 pKa = 11.84 VKK251 pKa = 10.34 RR252 pKa = 11.84 QRR254 pKa = 11.84 QADD257 pKa = 3.92 SPSGRR262 pKa = 11.84 RR263 pKa = 11.84 GRR265 pKa = 11.84 PWSEE269 pKa = 3.49 STPRR273 pKa = 11.84 NGRR276 pKa = 11.84 GGRR279 pKa = 11.84 EE280 pKa = 3.81 QRR282 pKa = 11.84 EE283 pKa = 4.21 PSPDD287 pKa = 3.18 TSPINGGGLSYY298 pKa = 10.95 DD299 pKa = 3.86 QIGSSHH305 pKa = 4.67 TTVPRR310 pKa = 11.84 RR311 pKa = 11.84 GIGRR315 pKa = 11.84 GQRR318 pKa = 11.84 LLLEE322 pKa = 4.64 ARR324 pKa = 11.84 DD325 pKa = 3.92 PPVILITGPANSLKK339 pKa = 10.12 CWRR342 pKa = 11.84 NRR344 pKa = 11.84 VRR346 pKa = 11.84 RR347 pKa = 11.84 RR348 pKa = 11.84 PVRR351 pKa = 11.84 LFDD354 pKa = 5.45 RR355 pKa = 11.84 ISTCFSWVTEE365 pKa = 4.02 GSGDD369 pKa = 3.5 GAGTQRR375 pKa = 11.84 LLIAFKK381 pKa = 11.02 DD382 pKa = 3.55 EE383 pKa = 4.2 TQRR386 pKa = 11.84 EE387 pKa = 4.5 LFLKK391 pKa = 8.46 TVPLPRR397 pKa = 11.84 GSKK400 pKa = 9.77 FFKK403 pKa = 10.94 GNLDD407 pKa = 3.63 GLL409 pKa = 4.27
Molecular weight: 46.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.303
IPC2_protein 8.302
IPC_protein 8.317
Toseland 8.785
ProMoST 8.712
Dawson 9.121
Bjellqvist 8.96
Wikipedia 9.311
Rodwell 9.224
Grimsley 9.165
Solomon 9.224
Lehninger 9.18
Nozaki 8.946
DTASelect 8.902
Thurlkill 8.975
EMBOSS 9.238
Sillero 9.121
Patrickios 4.736
IPC_peptide 9.209
IPC2_peptide 7.629
IPC2.peptide.svr19 7.642
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2415
145
609
402.5
45.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.128 ± 0.786
1.988 ± 0.648
6.708 ± 0.373
6.087 ± 0.387
4.224 ± 0.48
7.122 ± 1.157
1.449 ± 0.101
4.679 ± 0.86
4.596 ± 0.875
8.737 ± 0.765
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.781 ± 0.411
3.892 ± 0.605
6.377 ± 0.784
4.555 ± 0.58
7.039 ± 0.79
7.329 ± 0.96
6.211 ± 0.578
6.708 ± 0.632
1.284 ± 0.408
3.106 ± 0.223
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here