Yersinia phage vB_YenP_AP10
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0P0LM15|A0A0P0LM15_9CAUD DNA ligase OS=Yersinia phage vB_YenP_AP10 OX=1735591 PE=4 SV=1
MM1 pKa = 7.48 AMSNMTYY8 pKa = 11.1 SNVFDD13 pKa = 4.46 HH14 pKa = 7.18 AYY16 pKa = 10.58 EE17 pKa = 3.9 MLNEE21 pKa = 4.3 NIRR24 pKa = 11.84 YY25 pKa = 9.79 DD26 pKa = 5.05 DD27 pKa = 3.94 IRR29 pKa = 11.84 DD30 pKa = 3.59 TDD32 pKa = 3.81 GLGDD36 pKa = 5.34 AIHH39 pKa = 6.6 EE40 pKa = 4.6 AADD43 pKa = 3.63 SAVPHH48 pKa = 6.4 YY49 pKa = 10.5 YY50 pKa = 10.65 SDD52 pKa = 3.44 IFSVMASEE60 pKa = 5.55 GIDD63 pKa = 4.5 LEE65 pKa = 4.76 FEE67 pKa = 4.73 DD68 pKa = 5.02 SGLMPDD74 pKa = 3.85 TKK76 pKa = 10.88 DD77 pKa = 3.22 VTSILQARR85 pKa = 11.84 IYY87 pKa = 9.82 EE88 pKa = 4.14 QLTIDD93 pKa = 3.75 LWEE96 pKa = 4.32 KK97 pKa = 11.36 AEE99 pKa = 4.51 DD100 pKa = 3.81 LLNEE104 pKa = 3.99 YY105 pKa = 10.52 LEE107 pKa = 4.29 EE108 pKa = 4.22 VEE110 pKa = 4.4 EE111 pKa = 4.3 EE112 pKa = 4.13 EE113 pKa = 4.71 EE114 pKa = 3.94 EE115 pKa = 4.52 RR116 pKa = 11.84 EE117 pKa = 4.0 EE118 pKa = 5.36 DD119 pKa = 3.8 EE120 pKa = 4.49 EE121 pKa = 4.39
Molecular weight: 14.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.774
IPC2_protein 3.694
IPC_protein 3.668
Toseland 3.478
ProMoST 3.795
Dawson 3.643
Bjellqvist 3.834
Wikipedia 3.541
Rodwell 3.503
Grimsley 3.389
Solomon 3.63
Lehninger 3.579
Nozaki 3.757
DTASelect 3.91
Thurlkill 3.516
EMBOSS 3.554
Sillero 3.783
Patrickios 1.812
IPC_peptide 3.63
IPC2_peptide 3.757
IPC2.peptide.svr19 3.733
Protein with the highest isoelectric point:
>tr|A0A0N7J838|A0A0N7J838_9CAUD Host RNA polymerase inhibitor OS=Yersinia phage vB_YenP_AP10 OX=1735591 PE=4 SV=1
MM1 pKa = 6.6 VTLFVITMYY10 pKa = 11.41 ALIGAYY16 pKa = 9.83 FMRR19 pKa = 11.84 DD20 pKa = 3.55 FKK22 pKa = 10.98 RR23 pKa = 11.84 GLMHH27 pKa = 7.18 HH28 pKa = 7.01 KK29 pKa = 9.7 ATLSHH34 pKa = 6.88 FKK36 pKa = 10.87 YY37 pKa = 10.57 GFIPRR42 pKa = 11.84 FTARR46 pKa = 11.84 EE47 pKa = 3.63 PNGRR51 pKa = 11.84 FKK53 pKa = 11.26 ANKK56 pKa = 9.56 VGIFYY61 pKa = 10.48 IATHH65 pKa = 6.18
Molecular weight: 7.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.284
IPC2_protein 10.175
IPC_protein 10.745
Toseland 10.672
ProMoST 10.35
Dawson 10.804
Bjellqvist 10.496
Wikipedia 11.008
Rodwell 11.184
Grimsley 10.877
Solomon 10.877
Lehninger 10.847
Nozaki 10.628
DTASelect 10.496
Thurlkill 10.687
EMBOSS 11.052
Sillero 10.716
Patrickios 10.95
IPC_peptide 10.877
IPC2_peptide 9.311
IPC2.peptide.svr19 8.153
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
47
0
47
11798
37
1325
251.0
27.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.408 ± 0.46
0.856 ± 0.184
6.442 ± 0.282
7.052 ± 0.375
3.772 ± 0.186
7.815 ± 0.357
1.873 ± 0.216
4.992 ± 0.188
6.577 ± 0.353
8.027 ± 0.264
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.89 ± 0.187
4.391 ± 0.32
3.645 ± 0.215
4.145 ± 0.369
5.518 ± 0.229
5.874 ± 0.332
5.238 ± 0.208
6.586 ± 0.369
1.517 ± 0.201
3.382 ± 0.195
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here