Chaetoceros protobacilladnavirus 3

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Baphyvirales; Bacilladnaviridae; Protobacilladnavirus

Average proteome isoelectric point is 8.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E9RFF0|E9RFF0_9VIRU Isoform of E9RFF1 Hypothetical protein protein OS=Chaetoceros protobacilladnavirus 3 OX=2170142 GN=hypothetical protein PE=4 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84QKK4 pKa = 10.65SINEE8 pKa = 3.85ILCNGLPQQEE18 pKa = 4.66LLPSEE23 pKa = 5.1SVFSDD28 pKa = 3.31SAEE31 pKa = 3.95SRR33 pKa = 11.84LLKK36 pKa = 10.44EE37 pKa = 4.05WVNDD41 pKa = 4.17FSDD44 pKa = 3.76SSPIGLDD51 pKa = 3.16LGSLDD56 pKa = 3.95TVPEE60 pKa = 3.91FDD62 pKa = 3.51VASSKK67 pKa = 10.45RR68 pKa = 11.84ASSLTTPGTLNPIQPLSRR86 pKa = 11.84PLVRR90 pKa = 11.84STAVDD95 pKa = 3.39MTPSEE100 pKa = 4.52TEE102 pKa = 4.11DD103 pKa = 3.69EE104 pKa = 4.49LSGTHH109 pKa = 6.46QDD111 pKa = 3.28PCSKK115 pKa = 10.44KK116 pKa = 10.26DD117 pKa = 3.37QLLSRR122 pKa = 11.84FILTFFPEE130 pKa = 4.3TKK132 pKa = 9.17EE133 pKa = 4.05DD134 pKa = 3.12RR135 pKa = 11.84WMEE138 pKa = 4.12PDD140 pKa = 3.27TYY142 pKa = 11.0FPGAVEE148 pKa = 5.09LFHH151 pKa = 6.87VWTGQYY157 pKa = 9.59EE158 pKa = 4.28EE159 pKa = 5.26CPTTKK164 pKa = 10.03RR165 pKa = 11.84LHH167 pKa = 4.92AHH169 pKa = 6.98IYY171 pKa = 10.89VEE173 pKa = 4.03MLTNRR178 pKa = 11.84RR179 pKa = 11.84KK180 pKa = 10.3RR181 pKa = 11.84FNAFHH186 pKa = 5.4KK187 pKa = 10.17HH188 pKa = 4.0IRR190 pKa = 11.84KK191 pKa = 8.34FHH193 pKa = 6.54KK194 pKa = 10.22GVQIRR199 pKa = 11.84SARR202 pKa = 11.84RR203 pKa = 11.84CSVKK207 pKa = 10.09QRR209 pKa = 11.84QCAINYY215 pKa = 9.41VLDD218 pKa = 3.69DD219 pKa = 4.04RR220 pKa = 11.84KK221 pKa = 10.44RR222 pKa = 11.84LEE224 pKa = 4.53GSDD227 pKa = 3.43TYY229 pKa = 9.63TWRR232 pKa = 11.84HH233 pKa = 4.29SHH235 pKa = 7.25FEE237 pKa = 3.89PKK239 pKa = 10.0YY240 pKa = 9.42DD241 pKa = 4.2SKK243 pKa = 11.47FEE245 pKa = 4.06KK246 pKa = 9.2TKK248 pKa = 10.81EE249 pKa = 3.85MVKK252 pKa = 10.28RR253 pKa = 11.84GEE255 pKa = 4.02DD256 pKa = 3.49SEE258 pKa = 4.46TQRR261 pKa = 11.84VYY263 pKa = 10.66IEE265 pKa = 4.26SKK267 pKa = 9.53PRR269 pKa = 11.84MWTWDD274 pKa = 3.62QIVHH278 pKa = 5.6EE279 pKa = 4.36TDD281 pKa = 2.96EE282 pKa = 4.63SKK284 pKa = 11.18KK285 pKa = 10.89LLFNCSWGNKK295 pKa = 6.06YY296 pKa = 10.14HH297 pKa = 7.02AGRR300 pKa = 11.84VAEE303 pKa = 4.19VSRR306 pKa = 11.84RR307 pKa = 11.84TIQQVIILYY316 pKa = 10.05GAGGTGKK323 pKa = 7.42TTTVQSWDD331 pKa = 3.14ARR333 pKa = 11.84EE334 pKa = 4.18NEE336 pKa = 4.37DD337 pKa = 3.5PRR339 pKa = 11.84EE340 pKa = 3.7RR341 pKa = 11.84YY342 pKa = 9.12YY343 pKa = 11.01RR344 pKa = 11.84RR345 pKa = 11.84NPDD348 pKa = 4.7DD349 pKa = 3.61GAFWGGGRR357 pKa = 11.84TAYY360 pKa = 10.09RR361 pKa = 11.84GQRR364 pKa = 11.84IVHH367 pKa = 5.34YY368 pKa = 10.43EE369 pKa = 3.82EE370 pKa = 4.32FTGQEE375 pKa = 3.72AFCRR379 pKa = 11.84LKK381 pKa = 10.54EE382 pKa = 3.9VCDD385 pKa = 3.78IGKK388 pKa = 9.21HH389 pKa = 4.64GPPVNVKK396 pKa = 9.74MGGTDD401 pKa = 3.55LNHH404 pKa = 7.08EE405 pKa = 4.09IVIFTTNVHH414 pKa = 5.7PAGWFHH420 pKa = 7.11KK421 pKa = 10.41LWANDD426 pKa = 3.48PKK428 pKa = 10.7QFHH431 pKa = 6.23PFWRR435 pKa = 11.84RR436 pKa = 11.84VTKK439 pKa = 10.49VMFYY443 pKa = 10.28PSHH446 pKa = 6.97RR447 pKa = 11.84PDD449 pKa = 3.4GQLNCPDD456 pKa = 4.89DD457 pKa = 3.91EE458 pKa = 5.02HH459 pKa = 7.02PPHH462 pKa = 7.63VVDD465 pKa = 5.44QTEE468 pKa = 4.03DD469 pKa = 3.13WQSFKK474 pKa = 11.29GDD476 pKa = 3.37YY477 pKa = 10.49AKK479 pKa = 10.8CLNHH483 pKa = 7.35AEE485 pKa = 4.42KK486 pKa = 10.36FWPLKK491 pKa = 10.49LEE493 pKa = 4.36PEE495 pKa = 4.31STPFSRR501 pKa = 11.84DD502 pKa = 3.06FGHH505 pKa = 7.03SRR507 pKa = 11.84GQTEE511 pKa = 3.66QSTPFFHH518 pKa = 6.42YY519 pKa = 9.8CKK521 pKa = 10.2SGRR524 pKa = 11.84DD525 pKa = 3.52PTKK528 pKa = 10.64RR529 pKa = 11.84SII531 pKa = 3.65

Molecular weight: 61.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E9RFF2|E9RFF2_9VIRU Replication-associated protein OS=Chaetoceros protobacilladnavirus 3 OX=2170142 PE=4 SV=1
MM1 pKa = 7.73AGRR4 pKa = 11.84RR5 pKa = 11.84KK6 pKa = 8.99QRR8 pKa = 11.84RR9 pKa = 11.84SRR11 pKa = 11.84APRR14 pKa = 11.84RR15 pKa = 11.84KK16 pKa = 8.62RR17 pKa = 11.84TTGRR21 pKa = 11.84KK22 pKa = 5.15TLKK25 pKa = 10.35RR26 pKa = 11.84KK27 pKa = 9.68ADD29 pKa = 3.55SRR31 pKa = 11.84ATPAKK36 pKa = 9.15STRR39 pKa = 11.84KK40 pKa = 8.0RR41 pKa = 11.84TKK43 pKa = 9.07TVSGAAKK50 pKa = 9.06HH51 pKa = 5.57HH52 pKa = 5.91AVRR55 pKa = 11.84VKK57 pKa = 10.18PFSNATTQPKK67 pKa = 10.07IPDD70 pKa = 3.75GLLTSSLSRR79 pKa = 11.84RR80 pKa = 11.84LQNVVGVRR88 pKa = 11.84NGNSPSVHH96 pKa = 6.34AGSDD100 pKa = 3.51VMHH103 pKa = 6.04VVIAPTLGVPVMIANSAEE121 pKa = 4.17GVLKK125 pKa = 10.65RR126 pKa = 11.84PGLSQEE132 pKa = 3.91SSFIGFPGQTVGFEE146 pKa = 4.1NLIEE150 pKa = 4.38STGVPTWPPTIPTGQKK166 pKa = 10.19LEE168 pKa = 4.23NKK170 pKa = 9.99GGFVLWRR177 pKa = 11.84IISQGLRR184 pKa = 11.84IDD186 pKa = 4.25LANSDD191 pKa = 4.54EE192 pKa = 4.75EE193 pKa = 4.21NDD195 pKa = 3.53GWFEE199 pKa = 3.94ACRR202 pKa = 11.84FNWRR206 pKa = 11.84NVPRR210 pKa = 11.84DD211 pKa = 3.34VCMTPLDD218 pKa = 4.04GSTTTNSIGIAPNPLWLEE236 pKa = 3.85EE237 pKa = 4.08VGYY240 pKa = 10.79GMAMVEE246 pKa = 3.98QPGYY250 pKa = 10.92KK251 pKa = 9.7SGLLKK256 pKa = 10.44DD257 pKa = 3.38IKK259 pKa = 10.47KK260 pKa = 10.68AEE262 pKa = 4.23FMLHH266 pKa = 6.39PRR268 pKa = 11.84TTTHH272 pKa = 7.37DD273 pKa = 3.53PTLIDD278 pKa = 3.59PFEE281 pKa = 4.69YY282 pKa = 10.27GGSMTSSGGIDD293 pKa = 3.17NVYY296 pKa = 10.17YY297 pKa = 10.3PSDD300 pKa = 3.78NVSGNAVRR308 pKa = 11.84FRR310 pKa = 11.84DD311 pKa = 3.49MGVDD315 pKa = 3.92QNMDD319 pKa = 3.53WIYY322 pKa = 10.88IRR324 pKa = 11.84LHH326 pKa = 5.82CRR328 pKa = 11.84PNNGTSSLGSNFLFNVIQNVEE349 pKa = 4.12VAFNPSSDD357 pKa = 3.18FAAFQTINKK366 pKa = 9.38ADD368 pKa = 3.85TKK370 pKa = 10.47TKK372 pKa = 9.93MVADD376 pKa = 4.47GLNNNPDD383 pKa = 3.35VFNGRR388 pKa = 11.84RR389 pKa = 11.84KK390 pKa = 10.0

Molecular weight: 42.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

1

3

1252

331

531

417.3

47.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.99 ± 1.353

1.198 ± 0.475

5.751 ± 0.347

5.911 ± 0.957

4.073 ± 0.755

7.268 ± 0.72

3.195 ± 0.634

4.473 ± 0.529

7.508 ± 1.187

6.47 ± 0.364

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.917 ± 0.323

4.633 ± 0.955

5.751 ± 0.59

3.834 ± 0.367

7.907 ± 0.181

7.348 ± 0.249

6.15 ± 0.916

6.15 ± 0.475

1.837 ± 0.445

2.636 ± 0.387
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski