Amino acid dipepetide frequency for Microcystis phage Me-ZS1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.338AlaAla: 12.338 ± 2.125
1.122AlaCys: 1.122 ± 0.262
5.542AlaAsp: 5.542 ± 0.637
6.994AlaGlu: 6.994 ± 0.908
3.959AlaPhe: 3.959 ± 0.556
8.38AlaGly: 8.38 ± 1.075
1.386AlaHis: 1.386 ± 0.309
4.619AlaIle: 4.619 ± 0.779
5.344AlaLys: 5.344 ± 0.635
10.557AlaLeu: 10.557 ± 1.034
2.639AlaMet: 2.639 ± 0.359
3.431AlaAsn: 3.431 ± 0.395
6.4AlaPro: 6.4 ± 1.548
4.751AlaGln: 4.751 ± 0.747
6.202AlaArg: 6.202 ± 0.522
6.532AlaSer: 6.532 ± 0.676
6.862AlaThr: 6.862 ± 0.946
7.126AlaVal: 7.126 ± 0.692
1.32AlaTrp: 1.32 ± 0.278
3.035AlaTyr: 3.035 ± 0.485
0.0AlaXaa: 0.0 ± 0.0
Cys
1.452CysAla: 1.452 ± 0.4
0.198CysCys: 0.198 ± 0.116
0.66CysAsp: 0.66 ± 0.253
0.726CysGlu: 0.726 ± 0.221
0.33CysPhe: 0.33 ± 0.174
0.594CysGly: 0.594 ± 0.215
0.264CysHis: 0.264 ± 0.123
0.462CysIle: 0.462 ± 0.18
0.396CysLys: 0.396 ± 0.17
0.858CysLeu: 0.858 ± 0.267
0.132CysMet: 0.132 ± 0.096
0.462CysAsn: 0.462 ± 0.169
0.792CysPro: 0.792 ± 0.326
0.066CysGln: 0.066 ± 0.059
1.122CysArg: 1.122 ± 0.287
0.594CysSer: 0.594 ± 0.231
0.726CysThr: 0.726 ± 0.289
0.99CysVal: 0.99 ± 0.34
0.264CysTrp: 0.264 ± 0.115
0.264CysTyr: 0.264 ± 0.12
0.0CysXaa: 0.0 ± 0.0
Asp
5.608AspAla: 5.608 ± 0.593
0.66AspCys: 0.66 ± 0.203
2.441AspAsp: 2.441 ± 0.592
3.959AspGlu: 3.959 ± 0.542
1.715AspPhe: 1.715 ± 0.299
3.695AspGly: 3.695 ± 0.47
1.188AspHis: 1.188 ± 0.316
3.101AspIle: 3.101 ± 0.515
2.111AspLys: 2.111 ± 0.336
4.685AspLeu: 4.685 ± 0.507
0.726AspMet: 0.726 ± 0.223
1.386AspAsn: 1.386 ± 0.285
2.507AspPro: 2.507 ± 0.265
1.518AspGln: 1.518 ± 0.36
3.497AspArg: 3.497 ± 0.504
3.431AspSer: 3.431 ± 0.476
3.761AspThr: 3.761 ± 0.44
4.553AspVal: 4.553 ± 0.516
0.858AspTrp: 0.858 ± 0.272
1.979AspTyr: 1.979 ± 0.344
0.0AspXaa: 0.0 ± 0.0
Glu
8.973GluAla: 8.973 ± 0.923
0.726GluCys: 0.726 ± 0.204
2.969GluAsp: 2.969 ± 0.549
4.289GluGlu: 4.289 ± 0.648
2.639GluPhe: 2.639 ± 0.471
4.355GluGly: 4.355 ± 0.596
1.452GluHis: 1.452 ± 0.293
2.573GluIle: 2.573 ± 0.367
2.837GluLys: 2.837 ± 0.428
7.522GluLeu: 7.522 ± 1.209
1.913GluMet: 1.913 ± 0.384
1.847GluAsn: 1.847 ± 0.446
2.243GluPro: 2.243 ± 0.469
3.563GluGln: 3.563 ± 0.587
4.157GluArg: 4.157 ± 0.548
3.101GluSer: 3.101 ± 0.413
4.025GluThr: 4.025 ± 0.653
5.015GluVal: 5.015 ± 0.483
0.528GluTrp: 0.528 ± 0.192
1.386GluTyr: 1.386 ± 0.239
0.0GluXaa: 0.0 ± 0.0
Phe
4.025PheAla: 4.025 ± 0.457
0.462PheCys: 0.462 ± 0.164
2.771PheAsp: 2.771 ± 0.421
2.177PheGlu: 2.177 ± 0.286
0.924PhePhe: 0.924 ± 0.273
4.025PheGly: 4.025 ± 0.55
0.726PheHis: 0.726 ± 0.256
1.188PheIle: 1.188 ± 0.279
1.254PheLys: 1.254 ± 0.334
2.111PheLeu: 2.111 ± 0.333
0.726PheMet: 0.726 ± 0.21
1.452PheAsn: 1.452 ± 0.278
1.32PhePro: 1.32 ± 0.288
0.66PheGln: 0.66 ± 0.273
2.375PheArg: 2.375 ± 0.356
2.375PheSer: 2.375 ± 0.429
2.441PheThr: 2.441 ± 0.326
3.101PheVal: 3.101 ± 0.39
0.594PheTrp: 0.594 ± 0.168
0.726PheTyr: 0.726 ± 0.238
0.0PheXaa: 0.0 ± 0.0
Gly
7.39GlyAla: 7.39 ± 0.758
0.792GlyCys: 0.792 ± 0.231
3.959GlyAsp: 3.959 ± 0.43
4.289GlyGlu: 4.289 ± 0.611
2.573GlyPhe: 2.573 ± 0.387
6.202GlyGly: 6.202 ± 0.828
1.715GlyHis: 1.715 ± 0.331
4.223GlyIle: 4.223 ± 0.456
3.497GlyLys: 3.497 ± 0.6
7.192GlyLeu: 7.192 ± 0.896
2.705GlyMet: 2.705 ± 0.38
2.243GlyAsn: 2.243 ± 0.455
3.563GlyPro: 3.563 ± 0.444
2.111GlyGln: 2.111 ± 0.406
5.542GlyArg: 5.542 ± 0.653
4.619GlySer: 4.619 ± 0.621
5.806GlyThr: 5.806 ± 0.583
5.41GlyVal: 5.41 ± 0.564
1.781GlyTrp: 1.781 ± 0.375
2.441GlyTyr: 2.441 ± 0.412
0.0GlyXaa: 0.0 ± 0.0
His
1.122HisAla: 1.122 ± 0.366
0.33HisCys: 0.33 ± 0.197
0.792HisAsp: 0.792 ± 0.228
1.452HisGlu: 1.452 ± 0.312
1.056HisPhe: 1.056 ± 0.287
1.979HisGly: 1.979 ± 0.408
0.594HisHis: 0.594 ± 0.241
0.858HisIle: 0.858 ± 0.216
1.122HisLys: 1.122 ± 0.282
1.386HisLeu: 1.386 ± 0.315
0.33HisMet: 0.33 ± 0.156
0.792HisAsn: 0.792 ± 0.276
1.056HisPro: 1.056 ± 0.324
0.462HisGln: 0.462 ± 0.161
1.254HisArg: 1.254 ± 0.281
1.122HisSer: 1.122 ± 0.277
1.122HisThr: 1.122 ± 0.284
1.847HisVal: 1.847 ± 0.467
0.33HisTrp: 0.33 ± 0.198
0.726HisTyr: 0.726 ± 0.371
0.0HisXaa: 0.0 ± 0.0
Ile
5.015IleAla: 5.015 ± 0.561
0.396IleCys: 0.396 ± 0.167
3.035IleAsp: 3.035 ± 0.382
3.035IleGlu: 3.035 ± 0.421
0.924IlePhe: 0.924 ± 0.199
3.563IleGly: 3.563 ± 0.57
0.528IleHis: 0.528 ± 0.171
1.65IleIle: 1.65 ± 0.304
1.518IleLys: 1.518 ± 0.33
3.299IleLeu: 3.299 ± 0.457
0.66IleMet: 0.66 ± 0.19
1.584IleAsn: 1.584 ± 0.334
2.309IlePro: 2.309 ± 0.522
1.122IleGln: 1.122 ± 0.253
3.893IleArg: 3.893 ± 0.561
2.177IleSer: 2.177 ± 0.501
3.035IleThr: 3.035 ± 0.487
3.101IleVal: 3.101 ± 0.599
0.462IleTrp: 0.462 ± 0.18
0.792IleTyr: 0.792 ± 0.228
0.0IleXaa: 0.0 ± 0.0
Lys
4.619LysAla: 4.619 ± 0.532
0.264LysCys: 0.264 ± 0.14
2.045LysAsp: 2.045 ± 0.385
2.903LysGlu: 2.903 ± 0.559
1.254LysPhe: 1.254 ± 0.255
2.375LysGly: 2.375 ± 0.399
1.122LysHis: 1.122 ± 0.344
1.584LysIle: 1.584 ± 0.278
1.32LysLys: 1.32 ± 0.325
4.025LysLeu: 4.025 ± 0.801
0.99LysMet: 0.99 ± 0.288
1.979LysAsn: 1.979 ± 0.368
2.243LysPro: 2.243 ± 0.533
1.65LysGln: 1.65 ± 0.318
2.837LysArg: 2.837 ± 0.513
2.309LysSer: 2.309 ± 0.439
2.771LysThr: 2.771 ± 0.412
3.035LysVal: 3.035 ± 0.575
0.396LysTrp: 0.396 ± 0.2
0.99LysTyr: 0.99 ± 0.234
0.0LysXaa: 0.0 ± 0.0
Leu
10.029LeuAla: 10.029 ± 1.246
1.056LeuCys: 1.056 ± 0.31
6.598LeuAsp: 6.598 ± 0.547
5.938LeuGlu: 5.938 ± 0.649
3.233LeuPhe: 3.233 ± 0.393
6.334LeuGly: 6.334 ± 0.961
1.188LeuHis: 1.188 ± 0.407
3.233LeuIle: 3.233 ± 0.464
2.375LeuLys: 2.375 ± 0.367
7.324LeuLeu: 7.324 ± 0.825
1.913LeuMet: 1.913 ± 0.338
3.365LeuAsn: 3.365 ± 0.493
3.959LeuPro: 3.959 ± 0.648
4.223LeuGln: 4.223 ± 0.561
7.456LeuArg: 7.456 ± 0.744
5.08LeuSer: 5.08 ± 0.614
5.08LeuThr: 5.08 ± 0.487
6.664LeuVal: 6.664 ± 0.757
0.792LeuTrp: 0.792 ± 0.233
2.375LeuTyr: 2.375 ± 0.331
0.0LeuXaa: 0.0 ± 0.0
Met
1.65MetAla: 1.65 ± 0.293
0.396MetCys: 0.396 ± 0.172
1.122MetAsp: 1.122 ± 0.347
1.452MetGlu: 1.452 ± 0.263
0.594MetPhe: 0.594 ± 0.181
0.99MetGly: 0.99 ± 0.224
0.33MetHis: 0.33 ± 0.196
0.594MetIle: 0.594 ± 0.188
1.188MetLys: 1.188 ± 0.267
2.111MetLeu: 2.111 ± 0.418
0.198MetMet: 0.198 ± 0.14
0.99MetAsn: 0.99 ± 0.239
1.386MetPro: 1.386 ± 0.398
0.726MetGln: 0.726 ± 0.205
2.309MetArg: 2.309 ± 0.386
1.913MetSer: 1.913 ± 0.336
1.32MetThr: 1.32 ± 0.303
1.254MetVal: 1.254 ± 0.271
0.198MetTrp: 0.198 ± 0.104
0.858MetTyr: 0.858 ± 0.199
0.0MetXaa: 0.0 ± 0.0
Asn
4.421AsnAla: 4.421 ± 0.436
0.462AsnCys: 0.462 ± 0.172
1.254AsnAsp: 1.254 ± 0.26
2.507AsnGlu: 2.507 ± 0.364
0.858AsnPhe: 0.858 ± 0.186
3.893AsnGly: 3.893 ± 0.432
0.462AsnHis: 0.462 ± 0.177
1.65AsnIle: 1.65 ± 0.304
1.452AsnLys: 1.452 ± 0.264
2.573AsnLeu: 2.573 ± 0.563
0.528AsnMet: 0.528 ± 0.206
1.254AsnAsn: 1.254 ± 0.336
2.111AsnPro: 2.111 ± 0.442
1.781AsnGln: 1.781 ± 0.276
1.715AsnArg: 1.715 ± 0.222
1.584AsnSer: 1.584 ± 0.309
2.705AsnThr: 2.705 ± 0.535
2.177AsnVal: 2.177 ± 0.408
0.528AsnTrp: 0.528 ± 0.171
1.056AsnTyr: 1.056 ± 0.269
0.0AsnXaa: 0.0 ± 0.0
Pro
6.004ProAla: 6.004 ± 1.196
0.594ProCys: 0.594 ± 0.167
1.715ProAsp: 1.715 ± 0.419
3.299ProGlu: 3.299 ± 0.425
1.781ProPhe: 1.781 ± 0.268
3.299ProGly: 3.299 ± 0.505
1.122ProHis: 1.122 ± 0.272
2.045ProIle: 2.045 ± 0.481
2.507ProLys: 2.507 ± 0.543
3.695ProLeu: 3.695 ± 0.503
1.386ProMet: 1.386 ± 0.257
1.913ProAsn: 1.913 ± 0.38
2.837ProPro: 2.837 ± 0.593
1.32ProGln: 1.32 ± 0.406
3.827ProArg: 3.827 ± 0.711
2.903ProSer: 2.903 ± 0.401
3.563ProThr: 3.563 ± 0.617
3.959ProVal: 3.959 ± 0.577
0.726ProTrp: 0.726 ± 0.218
1.386ProTyr: 1.386 ± 0.346
0.0ProXaa: 0.0 ± 0.0
Gln
4.685GlnAla: 4.685 ± 1.018
0.396GlnCys: 0.396 ± 0.19
0.924GlnAsp: 0.924 ± 0.253
2.441GlnGlu: 2.441 ± 0.382
1.518GlnPhe: 1.518 ± 0.324
2.573GlnGly: 2.573 ± 0.271
0.528GlnHis: 0.528 ± 0.185
1.518GlnIle: 1.518 ± 0.353
1.386GlnLys: 1.386 ± 0.347
4.487GlnLeu: 4.487 ± 0.608
0.726GlnMet: 0.726 ± 0.189
1.715GlnAsn: 1.715 ± 0.295
2.177GlnPro: 2.177 ± 0.478
2.837GlnGln: 2.837 ± 0.918
2.903GlnArg: 2.903 ± 0.401
2.441GlnSer: 2.441 ± 0.568
1.847GlnThr: 1.847 ± 0.357
2.441GlnVal: 2.441 ± 0.406
1.056GlnTrp: 1.056 ± 0.331
0.792GlnTyr: 0.792 ± 0.196
0.0GlnXaa: 0.0 ± 0.0
Arg
7.192ArgAla: 7.192 ± 0.597
0.792ArgCys: 0.792 ± 0.202
3.761ArgAsp: 3.761 ± 0.477
5.344ArgGlu: 5.344 ± 0.766
2.639ArgPhe: 2.639 ± 0.434
5.146ArgGly: 5.146 ± 0.573
1.32ArgHis: 1.32 ± 0.339
3.365ArgIle: 3.365 ± 0.466
3.497ArgLys: 3.497 ± 0.664
6.598ArgLeu: 6.598 ± 0.677
1.781ArgMet: 1.781 ± 0.348
1.847ArgAsn: 1.847 ± 0.341
3.035ArgPro: 3.035 ± 0.436
2.837ArgGln: 2.837 ± 0.458
5.146ArgArg: 5.146 ± 0.699
4.091ArgSer: 4.091 ± 0.444
3.233ArgThr: 3.233 ± 0.421
5.41ArgVal: 5.41 ± 0.626
1.452ArgTrp: 1.452 ± 0.254
2.969ArgTyr: 2.969 ± 0.525
0.0ArgXaa: 0.0 ± 0.0
Ser
5.608SerAla: 5.608 ± 0.623
0.66SerCys: 0.66 ± 0.226
2.441SerAsp: 2.441 ± 0.388
3.167SerGlu: 3.167 ± 0.46
2.573SerPhe: 2.573 ± 0.354
5.212SerGly: 5.212 ± 0.641
1.056SerHis: 1.056 ± 0.269
2.243SerIle: 2.243 ± 0.436
2.771SerLys: 2.771 ± 0.463
4.421SerLeu: 4.421 ± 0.75
1.584SerMet: 1.584 ± 0.316
1.979SerAsn: 1.979 ± 0.37
2.771SerPro: 2.771 ± 0.448
1.847SerGln: 1.847 ± 0.392
3.563SerArg: 3.563 ± 0.442
3.827SerSer: 3.827 ± 0.71
4.091SerThr: 4.091 ± 0.441
5.476SerVal: 5.476 ± 0.462
0.858SerTrp: 0.858 ± 0.2
1.781SerTyr: 1.781 ± 0.319
0.0SerXaa: 0.0 ± 0.0
Thr
7.72ThrAla: 7.72 ± 1.171
0.462ThrCys: 0.462 ± 0.177
3.167ThrAsp: 3.167 ± 0.39
4.223ThrGlu: 4.223 ± 0.576
2.507ThrPhe: 2.507 ± 0.564
6.136ThrGly: 6.136 ± 0.64
1.452ThrHis: 1.452 ± 0.35
3.167ThrIle: 3.167 ± 0.516
2.507ThrLys: 2.507 ± 0.484
4.421ThrLeu: 4.421 ± 0.549
0.99ThrMet: 0.99 ± 0.3
2.375ThrAsn: 2.375 ± 0.378
3.431ThrPro: 3.431 ± 0.477
2.573ThrGln: 2.573 ± 0.354
3.431ThrArg: 3.431 ± 0.437
4.355ThrSer: 4.355 ± 0.54
3.629ThrThr: 3.629 ± 0.591
4.751ThrVal: 4.751 ± 0.638
0.726ThrTrp: 0.726 ± 0.211
1.452ThrTyr: 1.452 ± 0.294
0.0ThrXaa: 0.0 ± 0.0
Val
7.258ValAla: 7.258 ± 0.91
1.122ValCys: 1.122 ± 0.339
5.542ValAsp: 5.542 ± 0.564
5.344ValGlu: 5.344 ± 0.758
2.507ValPhe: 2.507 ± 0.473
5.212ValGly: 5.212 ± 0.628
2.045ValHis: 2.045 ± 0.381
2.111ValIle: 2.111 ± 0.345
2.705ValLys: 2.705 ± 0.506
6.73ValLeu: 6.73 ± 0.823
1.056ValMet: 1.056 ± 0.256
2.441ValAsn: 2.441 ± 0.411
4.025ValPro: 4.025 ± 0.536
3.893ValGln: 3.893 ± 0.522
6.664ValArg: 6.664 ± 0.644
2.837ValSer: 2.837 ± 0.365
4.685ValThr: 4.685 ± 0.53
4.553ValVal: 4.553 ± 0.567
1.254ValTrp: 1.254 ± 0.28
1.979ValTyr: 1.979 ± 0.394
0.0ValXaa: 0.0 ± 0.0
Trp
1.518TrpAla: 1.518 ± 0.263
0.132TrpCys: 0.132 ± 0.091
0.66TrpAsp: 0.66 ± 0.247
0.99TrpGlu: 0.99 ± 0.209
0.858TrpPhe: 0.858 ± 0.274
0.924TrpGly: 0.924 ± 0.267
0.198TrpHis: 0.198 ± 0.129
0.264TrpIle: 0.264 ± 0.141
0.33TrpLys: 0.33 ± 0.14
1.584TrpLeu: 1.584 ± 0.332
0.198TrpMet: 0.198 ± 0.126
0.66TrpAsn: 0.66 ± 0.212
0.792TrpPro: 0.792 ± 0.244
0.594TrpGln: 0.594 ± 0.209
1.32TrpArg: 1.32 ± 0.244
0.924TrpSer: 0.924 ± 0.284
1.254TrpThr: 1.254 ± 0.337
0.726TrpVal: 0.726 ± 0.185
0.396TrpTrp: 0.396 ± 0.153
0.858TrpTyr: 0.858 ± 0.262
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.913TyrAla: 1.913 ± 0.327
0.33TyrCys: 0.33 ± 0.13
1.979TyrAsp: 1.979 ± 0.361
1.584TyrGlu: 1.584 ± 0.333
0.99TyrPhe: 0.99 ± 0.306
3.167TyrGly: 3.167 ± 0.498
1.056TyrHis: 1.056 ± 0.248
1.65TyrIle: 1.65 ± 0.44
0.594TyrLys: 0.594 ± 0.218
2.639TyrLeu: 2.639 ± 0.536
0.33TyrMet: 0.33 ± 0.138
1.254TyrAsn: 1.254 ± 0.323
0.924TyrPro: 0.924 ± 0.266
0.858TyrGln: 0.858 ± 0.262
2.309TyrArg: 2.309 ± 0.433
1.715TyrSer: 1.715 ± 0.366
1.518TyrThr: 1.518 ± 0.371
2.375TyrVal: 2.375 ± 0.494
0.66TyrTrp: 0.66 ± 0.208
0.726TyrTyr: 0.726 ± 0.178
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 73 proteins (15157 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski