Roseburia sp. AM16-25

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Roseburia; unclassified Roseburia

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2357 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3R6HWF4|A0A3R6HWF4_9FIRM Uncharacterized protein OS=Roseburia sp. AM16-25 OX=2292065 GN=DW183_11580 PE=4 SV=1
MM1 pKa = 7.43EE2 pKa = 4.75VFLTMLNIKK11 pKa = 10.17RR12 pKa = 11.84FACTGLAAILVVSLPACGKK31 pKa = 9.93DD32 pKa = 3.04KK33 pKa = 11.35SSGYY37 pKa = 8.78TPLPEE42 pKa = 5.15EE43 pKa = 5.55DD44 pKa = 3.38DD45 pKa = 3.72TVYY48 pKa = 11.03NISLCQDD55 pKa = 3.3EE56 pKa = 5.87DD57 pKa = 3.48SDD59 pKa = 4.62YY60 pKa = 11.55YY61 pKa = 11.98NNISQGFNDD70 pKa = 4.41ALTDD74 pKa = 4.01LFGSAHH80 pKa = 5.64VNVTTMVANDD90 pKa = 4.17TIGTDD95 pKa = 4.23SICADD100 pKa = 3.96YY101 pKa = 10.15VTGGTDD107 pKa = 4.82LIFANGKK114 pKa = 9.9KK115 pKa = 9.91SLSSAATATEE125 pKa = 4.97DD126 pKa = 3.5IPIVGAAVMDD136 pKa = 4.15YY137 pKa = 11.15QSVLHH142 pKa = 6.75LATASDD148 pKa = 3.87SSWNKK153 pKa = 8.66KK154 pKa = 8.22TGTNVTGISSKK165 pKa = 10.93PNLEE169 pKa = 4.14EE170 pKa = 3.82QLSLLIEE177 pKa = 4.37ATPDD181 pKa = 3.46LQSVGLLYY189 pKa = 10.93NPEE192 pKa = 4.25DD193 pKa = 3.56TDD195 pKa = 4.94GIYY198 pKa = 10.76QNVLLEE204 pKa = 4.62KK205 pKa = 10.66YY206 pKa = 9.81LDD208 pKa = 3.58QAGIPWKK215 pKa = 10.34EE216 pKa = 3.76YY217 pKa = 10.94ALPSDD222 pKa = 5.66DD223 pKa = 4.19IDD225 pKa = 4.68SSISDD230 pKa = 4.0EE231 pKa = 4.37LTDD234 pKa = 3.64ATAIAPTKK242 pKa = 10.34QIASSVTEE250 pKa = 3.84GSNNDD255 pKa = 3.3VVSFAGNDD263 pKa = 3.55LLSGIFSPSSAHH275 pKa = 5.8VASTSATWTPDD286 pKa = 3.11LSIANAEE293 pKa = 4.19PLAADD298 pKa = 4.3ASLEE302 pKa = 4.66DD303 pKa = 3.54IVQYY307 pKa = 11.22ACNEE311 pKa = 4.02CSVLFLPAEE320 pKa = 4.23SQLTSEE326 pKa = 4.46VQTIVDD332 pKa = 3.45IATASGTRR340 pKa = 11.84TVGGDD345 pKa = 3.04ASLGQEE351 pKa = 4.57TLVSMYY357 pKa = 10.39KK358 pKa = 10.39DD359 pKa = 4.19PYY361 pKa = 10.29AMGYY365 pKa = 10.04AAGKK369 pKa = 7.19MVYY372 pKa = 10.03RR373 pKa = 11.84ILVDD377 pKa = 3.56GADD380 pKa = 3.91PGDD383 pKa = 3.52IKK385 pKa = 10.34ITNSPAEE392 pKa = 4.0NVKK395 pKa = 10.53LYY397 pKa = 10.04NASRR401 pKa = 11.84AEE403 pKa = 4.08SLGMTFPKK411 pKa = 10.28SFHH414 pKa = 6.9EE415 pKa = 4.4INDD418 pKa = 3.17YY419 pKa = 9.45FANYY423 pKa = 9.49EE424 pKa = 4.02IGSTTTRR431 pKa = 11.84ISSDD435 pKa = 3.3DD436 pKa = 3.44TAEE439 pKa = 3.8

Molecular weight: 46.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3R6MKB2|A0A3R6MKB2_9FIRM tRNA-specific 2-thiouridylase MnmA OS=Roseburia sp. AM16-25 OX=2292065 GN=mnmA PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.95KK9 pKa = 7.58RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.0VHH16 pKa = 5.93GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTAGGRR28 pKa = 11.84KK29 pKa = 8.71VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.1GRR39 pKa = 11.84KK40 pKa = 8.83KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2357

0

2357

795901

37

2652

337.7

37.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.912 ± 0.048

1.478 ± 0.022

6.115 ± 0.041

7.265 ± 0.049

3.986 ± 0.035

7.023 ± 0.046

1.929 ± 0.022

7.181 ± 0.043

6.491 ± 0.043

8.913 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.373 ± 0.027

4.046 ± 0.038

3.164 ± 0.028

3.591 ± 0.03

4.276 ± 0.036

5.61 ± 0.04

5.43 ± 0.045

6.986 ± 0.041

0.926 ± 0.017

4.306 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski