Streptococcus phage Javan648

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6BD15|A0A4D6BD15_9CAUD HicB-like domain-containing protein OS=Streptococcus phage Javan648 OX=2548299 GN=Javan648_0029 PE=4 SV=1
MM1 pKa = 7.17TVEE4 pKa = 4.39KK5 pKa = 10.75DD6 pKa = 4.37DD7 pKa = 5.48VILTLNLDD15 pKa = 3.55EE16 pKa = 6.19SDD18 pKa = 4.98DD19 pKa = 3.91IEE21 pKa = 6.16LIPNYY26 pKa = 9.44IKK28 pKa = 10.71SAEE31 pKa = 4.02IFIKK35 pKa = 10.75NAVGGDD41 pKa = 3.25DD42 pKa = 3.77KK43 pKa = 11.26FYY45 pKa = 11.02SKK47 pKa = 11.44EE48 pKa = 3.95NVTYY52 pKa = 10.46LYY54 pKa = 9.2DD55 pKa = 3.68TAVLSLASTYY65 pKa = 8.1YY66 pKa = 9.4TYY68 pKa = 10.78RR69 pKa = 11.84IALSDD74 pKa = 3.43TTTYY78 pKa = 10.77PIDD81 pKa = 3.54LTVNSIIGQLRR92 pKa = 11.84GLYY95 pKa = 9.98SIYY98 pKa = 10.74SEE100 pKa = 5.14GEE102 pKa = 3.8INN104 pKa = 4.15

Molecular weight: 11.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6BDZ4|A0A4D6BDZ4_9CAUD Uncharacterized protein OS=Streptococcus phage Javan648 OX=2548299 GN=Javan648_0004 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.15NGPKK7 pKa = 10.23GGGRR11 pKa = 11.84IGAVKK16 pKa = 10.31GRR18 pKa = 11.84SQSHH22 pKa = 5.0NPKK25 pKa = 9.14TGLYY29 pKa = 7.7TKK31 pKa = 10.29RR32 pKa = 11.84DD33 pKa = 3.38TSTGKK38 pKa = 10.62FMDD41 pKa = 3.68TKK43 pKa = 8.85TTGGKK48 pKa = 9.61FKK50 pKa = 10.73GVRR53 pKa = 11.84TEE55 pKa = 3.81KK56 pKa = 10.97

Molecular weight: 5.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

12221

37

1598

191.0

21.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.039 ± 0.423

0.425 ± 0.08

6.006 ± 0.295

6.824 ± 0.455

4.32 ± 0.238

5.744 ± 0.457

1.587 ± 0.149

7.544 ± 0.27

8.862 ± 0.472

8.633 ± 0.372

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.496 ± 0.124

6.882 ± 0.274

2.962 ± 0.183

4.075 ± 0.229

3.437 ± 0.202

7.021 ± 0.333

6.219 ± 0.378

5.671 ± 0.178

1.162 ± 0.103

4.091 ± 0.312
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski