Amino acid dipepetide frequency for Escherichia phage vB_EcoM_ECO1230-10

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.961AlaAla: 10.961 ± 1.116
0.855AlaCys: 0.855 ± 0.229
6.452AlaAsp: 6.452 ± 0.905
7.229AlaGlu: 7.229 ± 0.776
3.576AlaPhe: 3.576 ± 0.695
9.562AlaGly: 9.562 ± 0.97
0.933AlaHis: 0.933 ± 0.293
6.452AlaIle: 6.452 ± 0.901
5.752AlaLys: 5.752 ± 0.681
7.307AlaLeu: 7.307 ± 0.785
2.721AlaMet: 2.721 ± 0.485
6.141AlaAsn: 6.141 ± 0.674
4.042AlaPro: 4.042 ± 0.609
4.742AlaGln: 4.742 ± 0.739
5.752AlaArg: 5.752 ± 0.549
4.353AlaSer: 4.353 ± 0.491
6.919AlaThr: 6.919 ± 0.829
6.374AlaVal: 6.374 ± 0.884
1.71AlaTrp: 1.71 ± 0.373
3.42AlaTyr: 3.42 ± 0.561
0.0AlaXaa: 0.0 ± 0.0
Cys
0.544CysAla: 0.544 ± 0.227
0.155CysCys: 0.155 ± 0.099
1.088CysAsp: 1.088 ± 0.323
0.855CysGlu: 0.855 ± 0.256
0.233CysPhe: 0.233 ± 0.116
0.622CysGly: 0.622 ± 0.257
0.155CysHis: 0.155 ± 0.089
0.311CysIle: 0.311 ± 0.157
0.622CysLys: 0.622 ± 0.215
1.011CysLeu: 1.011 ± 0.315
0.078CysMet: 0.078 ± 0.087
0.233CysAsn: 0.233 ± 0.131
0.855CysPro: 0.855 ± 0.242
0.466CysGln: 0.466 ± 0.189
0.622CysArg: 0.622 ± 0.305
0.622CysSer: 0.622 ± 0.209
0.933CysThr: 0.933 ± 0.303
0.933CysVal: 0.933 ± 0.209
0.311CysTrp: 0.311 ± 0.148
0.233CysTyr: 0.233 ± 0.148
0.0CysXaa: 0.0 ± 0.0
Asp
5.442AspAla: 5.442 ± 0.577
0.466AspCys: 0.466 ± 0.174
4.275AspAsp: 4.275 ± 0.681
3.965AspGlu: 3.965 ± 0.569
2.954AspPhe: 2.954 ± 0.479
6.141AspGly: 6.141 ± 0.622
1.399AspHis: 1.399 ± 0.363
3.187AspIle: 3.187 ± 0.471
2.565AspLys: 2.565 ± 0.339
4.198AspLeu: 4.198 ± 0.411
1.943AspMet: 1.943 ± 0.325
2.565AspAsn: 2.565 ± 0.376
3.109AspPro: 3.109 ± 0.528
2.643AspGln: 2.643 ± 0.448
2.643AspArg: 2.643 ± 0.553
2.799AspSer: 2.799 ± 0.545
3.887AspThr: 3.887 ± 0.617
4.586AspVal: 4.586 ± 0.615
0.933AspTrp: 0.933 ± 0.254
2.021AspTyr: 2.021 ± 0.369
0.0AspXaa: 0.0 ± 0.0
Glu
5.597GluAla: 5.597 ± 0.709
1.088GluCys: 1.088 ± 0.276
1.322GluAsp: 1.322 ± 0.314
3.809GluGlu: 3.809 ± 0.755
1.555GluPhe: 1.555 ± 0.367
4.353GluGly: 4.353 ± 0.485
1.244GluHis: 1.244 ± 0.287
4.586GluIle: 4.586 ± 0.679
3.343GluLys: 3.343 ± 0.668
6.297GluLeu: 6.297 ± 0.788
1.943GluMet: 1.943 ± 0.362
1.71GluAsn: 1.71 ± 0.255
2.254GluPro: 2.254 ± 0.408
3.343GluGln: 3.343 ± 0.508
4.431GluArg: 4.431 ± 0.455
3.731GluSer: 3.731 ± 0.529
3.265GluThr: 3.265 ± 0.616
3.576GluVal: 3.576 ± 0.458
2.099GluTrp: 2.099 ± 0.394
1.866GluTyr: 1.866 ± 0.461
0.0GluXaa: 0.0 ± 0.0
Phe
3.809PheAla: 3.809 ± 0.616
0.311PheCys: 0.311 ± 0.14
2.332PheAsp: 2.332 ± 0.478
2.099PheGlu: 2.099 ± 0.328
0.544PhePhe: 0.544 ± 0.158
2.799PheGly: 2.799 ± 0.429
0.311PheHis: 0.311 ± 0.164
2.332PheIle: 2.332 ± 0.468
1.788PheLys: 1.788 ± 0.332
2.099PheLeu: 2.099 ± 0.445
1.088PheMet: 1.088 ± 0.317
2.254PheAsn: 2.254 ± 0.498
0.7PhePro: 0.7 ± 0.245
1.011PheGln: 1.011 ± 0.306
2.177PheArg: 2.177 ± 0.329
1.399PheSer: 1.399 ± 0.288
2.565PheThr: 2.565 ± 0.428
1.399PheVal: 1.399 ± 0.304
0.466PheTrp: 0.466 ± 0.169
0.466PheTyr: 0.466 ± 0.195
0.0PheXaa: 0.0 ± 0.0
Gly
7.929GlyAla: 7.929 ± 0.724
0.933GlyCys: 0.933 ± 0.354
4.198GlyAsp: 4.198 ± 0.593
5.053GlyGlu: 5.053 ± 0.51
3.109GlyPhe: 3.109 ± 0.46
7.774GlyGly: 7.774 ± 0.686
1.244GlyHis: 1.244 ± 0.339
4.742GlyIle: 4.742 ± 0.593
5.519GlyLys: 5.519 ± 0.521
4.586GlyLeu: 4.586 ± 0.525
1.943GlyMet: 1.943 ± 0.364
3.809GlyAsn: 3.809 ± 0.525
2.565GlyPro: 2.565 ± 0.415
3.187GlyGln: 3.187 ± 0.444
4.12GlyArg: 4.12 ± 0.671
4.586GlySer: 4.586 ± 0.583
5.752GlyThr: 5.752 ± 0.938
5.519GlyVal: 5.519 ± 0.571
1.788GlyTrp: 1.788 ± 0.399
3.343GlyTyr: 3.343 ± 0.427
0.0GlyXaa: 0.0 ± 0.0
His
1.477HisAla: 1.477 ± 0.347
0.622HisCys: 0.622 ± 0.215
0.933HisAsp: 0.933 ± 0.281
0.933HisGlu: 0.933 ± 0.228
0.855HisPhe: 0.855 ± 0.284
1.477HisGly: 1.477 ± 0.381
0.389HisHis: 0.389 ± 0.161
0.622HisIle: 0.622 ± 0.202
0.7HisLys: 0.7 ± 0.262
1.088HisLeu: 1.088 ± 0.285
0.7HisMet: 0.7 ± 0.232
0.311HisAsn: 0.311 ± 0.15
0.622HisPro: 0.622 ± 0.229
0.544HisGln: 0.544 ± 0.198
1.244HisArg: 1.244 ± 0.317
1.088HisSer: 1.088 ± 0.36
0.855HisThr: 0.855 ± 0.261
1.088HisVal: 1.088 ± 0.31
0.155HisTrp: 0.155 ± 0.104
0.466HisTyr: 0.466 ± 0.172
0.0HisXaa: 0.0 ± 0.0
Ile
6.141IleAla: 6.141 ± 0.778
0.622IleCys: 0.622 ± 0.231
5.053IleAsp: 5.053 ± 0.505
3.109IleGlu: 3.109 ± 0.568
1.71IlePhe: 1.71 ± 0.373
5.208IleGly: 5.208 ± 0.711
0.622IleHis: 0.622 ± 0.229
3.032IleIle: 3.032 ± 0.538
2.177IleLys: 2.177 ± 0.461
2.643IleLeu: 2.643 ± 0.415
1.166IleMet: 1.166 ± 0.254
2.332IleAsn: 2.332 ± 0.455
1.788IlePro: 1.788 ± 0.327
1.632IleGln: 1.632 ± 0.355
3.265IleArg: 3.265 ± 0.458
3.731IleSer: 3.731 ± 0.599
4.975IleThr: 4.975 ± 0.624
3.42IleVal: 3.42 ± 0.629
0.7IleTrp: 0.7 ± 0.163
1.088IleTyr: 1.088 ± 0.342
0.0IleXaa: 0.0 ± 0.0
Lys
4.431LysAla: 4.431 ± 0.729
0.622LysCys: 0.622 ± 0.236
3.265LysAsp: 3.265 ± 0.417
2.099LysGlu: 2.099 ± 0.511
1.866LysPhe: 1.866 ± 0.394
3.731LysGly: 3.731 ± 0.602
0.933LysHis: 0.933 ± 0.254
2.099LysIle: 2.099 ± 0.418
2.177LysLys: 2.177 ± 0.457
4.509LysLeu: 4.509 ± 0.5
1.632LysMet: 1.632 ± 0.412
1.788LysAsn: 1.788 ± 0.364
3.965LysPro: 3.965 ± 0.712
2.876LysGln: 2.876 ± 0.397
3.731LysArg: 3.731 ± 0.55
3.109LysSer: 3.109 ± 0.501
3.809LysThr: 3.809 ± 0.575
3.42LysVal: 3.42 ± 0.521
0.777LysTrp: 0.777 ± 0.259
1.088LysTyr: 1.088 ± 0.233
0.0LysXaa: 0.0 ± 0.0
Leu
9.484LeuAla: 9.484 ± 0.822
0.933LeuCys: 0.933 ± 0.242
4.586LeuAsp: 4.586 ± 0.615
5.131LeuGlu: 5.131 ± 0.604
2.41LeuPhe: 2.41 ± 0.457
4.82LeuGly: 4.82 ± 0.641
1.322LeuHis: 1.322 ± 0.288
3.498LeuIle: 3.498 ± 0.495
3.654LeuLys: 3.654 ± 0.539
5.053LeuLeu: 5.053 ± 0.556
1.866LeuMet: 1.866 ± 0.356
3.265LeuAsn: 3.265 ± 0.5
4.12LeuPro: 4.12 ± 0.519
2.488LeuGln: 2.488 ± 0.492
5.908LeuArg: 5.908 ± 0.566
3.965LeuSer: 3.965 ± 0.725
4.742LeuThr: 4.742 ± 0.661
5.597LeuVal: 5.597 ± 0.592
1.399LeuTrp: 1.399 ± 0.322
1.71LeuTyr: 1.71 ± 0.419
0.0LeuXaa: 0.0 ± 0.0
Met
4.198MetAla: 4.198 ± 0.451
0.0MetCys: 0.0 ± 0.0
1.244MetAsp: 1.244 ± 0.336
1.399MetGlu: 1.399 ± 0.288
0.7MetPhe: 0.7 ± 0.218
2.021MetGly: 2.021 ± 0.407
0.389MetHis: 0.389 ± 0.192
0.544MetIle: 0.544 ± 0.187
1.788MetLys: 1.788 ± 0.365
1.866MetLeu: 1.866 ± 0.27
0.777MetMet: 0.777 ± 0.222
1.788MetAsn: 1.788 ± 0.361
1.399MetPro: 1.399 ± 0.355
1.322MetGln: 1.322 ± 0.341
1.632MetArg: 1.632 ± 0.256
1.244MetSer: 1.244 ± 0.298
1.788MetThr: 1.788 ± 0.339
1.244MetVal: 1.244 ± 0.288
0.389MetTrp: 0.389 ± 0.143
0.078MetTyr: 0.078 ± 0.103
0.0MetXaa: 0.0 ± 0.0
Asn
4.975AsnAla: 4.975 ± 0.38
0.466AsnCys: 0.466 ± 0.175
3.809AsnAsp: 3.809 ± 0.612
2.643AsnGlu: 2.643 ± 0.466
0.855AsnPhe: 0.855 ± 0.241
3.187AsnGly: 3.187 ± 0.538
0.466AsnHis: 0.466 ± 0.184
2.643AsnIle: 2.643 ± 0.545
2.488AsnLys: 2.488 ± 0.419
3.42AsnLeu: 3.42 ± 0.595
0.855AsnMet: 0.855 ± 0.205
1.943AsnAsn: 1.943 ± 0.469
2.41AsnPro: 2.41 ± 0.427
1.244AsnGln: 1.244 ± 0.258
2.177AsnArg: 2.177 ± 0.391
2.021AsnSer: 2.021 ± 0.494
1.866AsnThr: 1.866 ± 0.404
3.265AsnVal: 3.265 ± 0.607
0.622AsnTrp: 0.622 ± 0.212
1.166AsnTyr: 1.166 ± 0.251
0.0AsnXaa: 0.0 ± 0.0
Pro
5.208ProAla: 5.208 ± 0.699
0.155ProCys: 0.155 ± 0.117
3.731ProAsp: 3.731 ± 0.493
2.954ProGlu: 2.954 ± 0.462
1.477ProPhe: 1.477 ± 0.284
3.498ProGly: 3.498 ± 0.556
0.7ProHis: 0.7 ± 0.241
1.399ProIle: 1.399 ± 0.304
2.721ProLys: 2.721 ± 0.5
3.265ProLeu: 3.265 ± 0.43
1.166ProMet: 1.166 ± 0.283
1.399ProAsn: 1.399 ± 0.252
2.41ProPro: 2.41 ± 0.527
2.021ProGln: 2.021 ± 0.395
2.177ProArg: 2.177 ± 0.448
2.41ProSer: 2.41 ± 0.499
3.032ProThr: 3.032 ± 0.48
3.42ProVal: 3.42 ± 0.528
0.7ProTrp: 0.7 ± 0.228
1.088ProTyr: 1.088 ± 0.233
0.0ProXaa: 0.0 ± 0.0
Gln
4.586GlnAla: 4.586 ± 0.737
0.311GlnCys: 0.311 ± 0.143
2.254GlnAsp: 2.254 ± 0.395
2.332GlnGlu: 2.332 ± 0.324
0.777GlnPhe: 0.777 ± 0.241
1.788GlnGly: 1.788 ± 0.405
1.088GlnHis: 1.088 ± 0.354
2.021GlnIle: 2.021 ± 0.445
2.254GlnLys: 2.254 ± 0.356
3.032GlnLeu: 3.032 ± 0.466
1.244GlnMet: 1.244 ± 0.25
1.088GlnAsn: 1.088 ± 0.254
2.254GlnPro: 2.254 ± 0.456
3.109GlnGln: 3.109 ± 0.479
3.265GlnArg: 3.265 ± 0.543
2.177GlnSer: 2.177 ± 0.323
2.099GlnThr: 2.099 ± 0.347
3.887GlnVal: 3.887 ± 0.474
0.7GlnTrp: 0.7 ± 0.205
1.088GlnTyr: 1.088 ± 0.226
0.0GlnXaa: 0.0 ± 0.0
Arg
6.685ArgAla: 6.685 ± 0.794
0.622ArgCys: 0.622 ± 0.234
3.498ArgAsp: 3.498 ± 0.52
4.742ArgGlu: 4.742 ± 0.562
1.71ArgPhe: 1.71 ± 0.332
3.809ArgGly: 3.809 ± 0.535
1.166ArgHis: 1.166 ± 0.339
3.343ArgIle: 3.343 ± 0.466
3.654ArgLys: 3.654 ± 0.63
5.675ArgLeu: 5.675 ± 0.743
1.322ArgMet: 1.322 ± 0.299
3.032ArgAsn: 3.032 ± 0.45
2.021ArgPro: 2.021 ± 0.299
2.099ArgGln: 2.099 ± 0.424
3.887ArgArg: 3.887 ± 0.554
3.654ArgSer: 3.654 ± 0.599
3.654ArgThr: 3.654 ± 0.507
2.876ArgVal: 2.876 ± 0.423
1.088ArgTrp: 1.088 ± 0.307
1.788ArgTyr: 1.788 ± 0.341
0.0ArgXaa: 0.0 ± 0.0
Ser
4.664SerAla: 4.664 ± 0.538
0.311SerCys: 0.311 ± 0.136
4.042SerAsp: 4.042 ± 0.547
2.565SerGlu: 2.565 ± 0.317
1.788SerPhe: 1.788 ± 0.459
5.131SerGly: 5.131 ± 0.679
0.777SerHis: 0.777 ± 0.22
3.42SerIle: 3.42 ± 0.537
2.488SerLys: 2.488 ± 0.388
5.752SerLeu: 5.752 ± 0.597
1.011SerMet: 1.011 ± 0.274
2.021SerAsn: 2.021 ± 0.429
2.643SerPro: 2.643 ± 0.436
2.177SerGln: 2.177 ± 0.43
2.876SerArg: 2.876 ± 0.395
4.198SerSer: 4.198 ± 0.974
3.032SerThr: 3.032 ± 0.577
4.275SerVal: 4.275 ± 0.725
1.011SerTrp: 1.011 ± 0.276
1.166SerTyr: 1.166 ± 0.282
0.0SerXaa: 0.0 ± 0.0
Thr
8.396ThrAla: 8.396 ± 0.955
0.777ThrCys: 0.777 ± 0.278
2.799ThrAsp: 2.799 ± 0.445
3.576ThrGlu: 3.576 ± 0.561
2.799ThrPhe: 2.799 ± 0.497
7.385ThrGly: 7.385 ± 0.609
0.933ThrHis: 0.933 ± 0.283
4.431ThrIle: 4.431 ± 0.584
2.799ThrLys: 2.799 ± 0.594
5.752ThrLeu: 5.752 ± 0.552
1.477ThrMet: 1.477 ± 0.41
1.943ThrAsn: 1.943 ± 0.421
2.954ThrPro: 2.954 ± 0.351
2.177ThrGln: 2.177 ± 0.395
2.876ThrArg: 2.876 ± 0.413
3.343ThrSer: 3.343 ± 0.451
4.198ThrThr: 4.198 ± 0.62
4.897ThrVal: 4.897 ± 0.691
0.855ThrTrp: 0.855 ± 0.296
1.555ThrTyr: 1.555 ± 0.412
0.0ThrXaa: 0.0 ± 0.0
Val
6.763ValAla: 6.763 ± 0.715
0.777ValCys: 0.777 ± 0.289
3.887ValAsp: 3.887 ± 0.689
4.664ValGlu: 4.664 ± 0.622
1.788ValPhe: 1.788 ± 0.39
4.975ValGly: 4.975 ± 0.658
0.777ValHis: 0.777 ± 0.242
3.809ValIle: 3.809 ± 0.611
3.654ValLys: 3.654 ± 0.503
5.053ValLeu: 5.053 ± 0.588
1.943ValMet: 1.943 ± 0.448
3.265ValAsn: 3.265 ± 0.591
2.799ValPro: 2.799 ± 0.459
2.021ValGln: 2.021 ± 0.497
4.353ValArg: 4.353 ± 0.717
3.731ValSer: 3.731 ± 0.49
5.752ValThr: 5.752 ± 0.688
5.986ValVal: 5.986 ± 0.935
0.933ValTrp: 0.933 ± 0.272
1.866ValTyr: 1.866 ± 0.5
0.0ValXaa: 0.0 ± 0.0
Trp
1.632TrpAla: 1.632 ± 0.329
0.155TrpCys: 0.155 ± 0.11
1.011TrpAsp: 1.011 ± 0.251
0.933TrpGlu: 0.933 ± 0.225
0.7TrpPhe: 0.7 ± 0.242
1.322TrpGly: 1.322 ± 0.379
0.622TrpHis: 0.622 ± 0.215
0.622TrpIle: 0.622 ± 0.242
0.855TrpLys: 0.855 ± 0.259
1.866TrpLeu: 1.866 ± 0.428
0.389TrpMet: 0.389 ± 0.159
0.7TrpAsn: 0.7 ± 0.231
0.777TrpPro: 0.777 ± 0.257
1.088TrpGln: 1.088 ± 0.291
1.166TrpArg: 1.166 ± 0.326
1.011TrpSer: 1.011 ± 0.259
1.088TrpThr: 1.088 ± 0.261
0.777TrpVal: 0.777 ± 0.221
0.233TrpTrp: 0.233 ± 0.154
0.233TrpTyr: 0.233 ± 0.124
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.332TyrAla: 2.332 ± 0.36
0.7TyrCys: 0.7 ± 0.217
1.943TyrAsp: 1.943 ± 0.441
1.322TyrGlu: 1.322 ± 0.344
0.622TyrPhe: 0.622 ± 0.216
2.099TyrGly: 2.099 ± 0.391
0.7TyrHis: 0.7 ± 0.225
1.322TyrIle: 1.322 ± 0.317
0.933TyrLys: 0.933 ± 0.278
1.477TyrLeu: 1.477 ± 0.371
0.466TyrMet: 0.466 ± 0.21
0.933TyrAsn: 0.933 ± 0.225
1.244TyrPro: 1.244 ± 0.251
1.166TyrGln: 1.166 ± 0.274
2.021TyrArg: 2.021 ± 0.346
2.177TyrSer: 2.177 ± 0.351
1.71TyrThr: 1.71 ± 0.322
2.254TyrVal: 2.254 ± 0.511
0.311TyrTrp: 0.311 ± 0.138
0.7TyrTyr: 0.7 ± 0.231
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 56 proteins (12865 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski