Nitrococcus mobilis Nb-231

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Ectothiorhodospiraceae; Nitrococcus; Nitrococcus mobilis

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3484 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A4BN85|A4BN85_9GAMM Glucose-6-phosphate 1-dehydrogenase OS=Nitrococcus mobilis Nb-231 OX=314278 GN=zwf PE=3 SV=1
MM1 pKa = 6.65TTWADD6 pKa = 4.32DD7 pKa = 3.15AWTLANEE14 pKa = 4.18AFVFVGPFHH23 pKa = 6.78NLDD26 pKa = 3.26IPSTFFHH33 pKa = 7.0CCDD36 pKa = 3.26SS37 pKa = 3.64

Molecular weight: 4.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A4BTX0|A4BTX0_9GAMM Glutamine synthetase OS=Nitrococcus mobilis Nb-231 OX=314278 GN=NB231_10959 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVIRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.58RR14 pKa = 11.84THH16 pKa = 6.01GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4AGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.36GRR39 pKa = 11.84ARR41 pKa = 11.84LIPP44 pKa = 4.0

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3484

0

3484

1019986

20

3074

292.8

32.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.357 ± 0.054

1.074 ± 0.015

5.166 ± 0.034

6.28 ± 0.045

3.419 ± 0.024

7.924 ± 0.048

2.461 ± 0.022

4.887 ± 0.034

2.829 ± 0.034

11.234 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.062 ± 0.017

2.626 ± 0.027

4.984 ± 0.032

4.053 ± 0.032

8.414 ± 0.048

5.115 ± 0.027

4.942 ± 0.03

7.116 ± 0.038

1.456 ± 0.019

2.601 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski