Escherichia phage HK544

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Hendrixvirinae; Kwaitsingvirus; Escherichia virus HK544

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K7P823|K7P823_9CAUD Major head subunit OS=Escherichia phage HK544 OX=432201 GN=HK544_005 PE=4 SV=1
MM1 pKa = 8.29DD2 pKa = 4.56YY3 pKa = 11.03SQLSDD8 pKa = 3.38FEE10 pKa = 4.82INKK13 pKa = 9.58RR14 pKa = 11.84VAICCGFAPEE24 pKa = 4.42DD25 pKa = 3.82CEE27 pKa = 4.06IAKK30 pKa = 10.45LGTSIVGVEE39 pKa = 3.81WDD41 pKa = 4.72DD42 pKa = 3.5EE43 pKa = 4.13TGYY46 pKa = 11.09AIKK49 pKa = 9.63TVDD52 pKa = 3.32YY53 pKa = 10.45CKK55 pKa = 10.28RR56 pKa = 11.84PSDD59 pKa = 3.32AWPIITEE66 pKa = 4.09NNISIILDD74 pKa = 3.81NPSMPCATDD83 pKa = 3.23NARR86 pKa = 11.84DD87 pKa = 3.79LFDD90 pKa = 5.03DD91 pKa = 4.5AGPNVGVAYY100 pKa = 9.96DD101 pKa = 3.76NPLRR105 pKa = 11.84AAMIVFLMIQDD116 pKa = 3.81ANNAA120 pKa = 3.77

Molecular weight: 13.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A4KWR1|A4KWR1_9CAUD Uncharacterized protein OS=Escherichia phage HK544 OX=432201 GN=HK544_040 PE=4 SV=1
MM1 pKa = 8.07DD2 pKa = 4.41AQTRR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84EE9 pKa = 3.77RR10 pKa = 11.84RR11 pKa = 11.84AEE13 pKa = 4.15KK14 pKa = 9.26QAQWKK19 pKa = 8.58AANPLLVGVSAKK31 pKa = 9.96PVNRR35 pKa = 11.84PILSLNRR42 pKa = 11.84KK43 pKa = 7.08PKK45 pKa = 10.54SRR47 pKa = 11.84VEE49 pKa = 4.08SALNPIDD56 pKa = 3.85LTVLAEE62 pKa = 3.84YY63 pKa = 10.59HH64 pKa = 5.65EE65 pKa = 4.71QIEE68 pKa = 4.84SNLQRR73 pKa = 11.84IEE75 pKa = 4.31RR76 pKa = 11.84KK77 pKa = 7.93NQRR80 pKa = 11.84TWYY83 pKa = 8.98SKK85 pKa = 9.72PRR87 pKa = 11.84SEE89 pKa = 4.9MGVTCVGRR97 pKa = 11.84QKK99 pKa = 10.24MKK101 pKa = 10.69LGSKK105 pKa = 9.97PLII108 pKa = 4.11

Molecular weight: 12.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

12179

32

1151

193.3

21.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.149 ± 0.567

1.166 ± 0.161

5.739 ± 0.245

6.462 ± 0.439

3.169 ± 0.16

7.143 ± 0.345

1.437 ± 0.195

5.748 ± 0.24

5.83 ± 0.386

7.776 ± 0.274

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.644 ± 0.155

4.828 ± 0.232

3.588 ± 0.261

4.656 ± 0.344

6.183 ± 0.326

6.856 ± 0.337

5.789 ± 0.373

6.388 ± 0.268

1.634 ± 0.143

2.816 ± 0.193
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski