Clostridium sp. CAG:780

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1194 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5Q7P1|R5Q7P1_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:780 OX=1262839 GN=BN780_00263 PE=4 SV=1
MM1 pKa = 7.51SKK3 pKa = 10.79LSNEE7 pKa = 3.87YY8 pKa = 10.68LKK10 pKa = 10.55IIEE13 pKa = 5.57DD14 pKa = 3.8IEE16 pKa = 4.07KK17 pKa = 10.38HH18 pKa = 5.45ISNKK22 pKa = 9.94EE23 pKa = 3.5EE24 pKa = 4.01SKK26 pKa = 11.19YY27 pKa = 6.79VTKK30 pKa = 10.8KK31 pKa = 10.19IGEE34 pKa = 4.14LTALYY39 pKa = 9.37MDD41 pKa = 6.1LIEE44 pKa = 5.56KK45 pKa = 10.15SINANLDD52 pKa = 3.59RR53 pKa = 11.84IEE55 pKa = 4.65EE56 pKa = 4.15IEE58 pKa = 4.1EE59 pKa = 3.87QLDD62 pKa = 3.81KK63 pKa = 11.49VQEE66 pKa = 4.26TVSLIKK72 pKa = 10.24KK73 pKa = 10.08DD74 pKa = 3.18IYY76 pKa = 10.95EE77 pKa = 4.53DD78 pKa = 3.49EE79 pKa = 5.86DD80 pKa = 4.53YY81 pKa = 11.29DD82 pKa = 4.64FEE84 pKa = 5.23IVCPYY89 pKa = 10.22CNHH92 pKa = 6.26EE93 pKa = 4.41FVADD97 pKa = 3.86VEE99 pKa = 5.05DD100 pKa = 3.88EE101 pKa = 4.14LKK103 pKa = 11.11EE104 pKa = 4.18EE105 pKa = 4.33IEE107 pKa = 5.02CPEE110 pKa = 3.9CHH112 pKa = 7.07NIIEE116 pKa = 4.55LDD118 pKa = 3.16WDD120 pKa = 3.77EE121 pKa = 5.06EE122 pKa = 4.38EE123 pKa = 5.21GCGGCCSSCGICDD136 pKa = 3.94EE137 pKa = 4.42EE138 pKa = 5.72DD139 pKa = 3.43EE140 pKa = 4.91EE141 pKa = 5.39EE142 pKa = 4.2NDD144 pKa = 4.57EE145 pKa = 4.48NDD147 pKa = 4.57DD148 pKa = 3.65MM149 pKa = 7.51

Molecular weight: 17.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5PT90|R5PT90_9CLOT Stage V sporulation protein AE OS=Clostridium sp. CAG:780 OX=1262839 GN=BN780_00792 PE=4 SV=1
MM1 pKa = 7.42ARR3 pKa = 11.84LAGVDD8 pKa = 3.64LPRR11 pKa = 11.84EE12 pKa = 3.93KK13 pKa = 10.21RR14 pKa = 11.84VEE16 pKa = 3.65IGLTYY21 pKa = 9.99IYY23 pKa = 10.59GIGLTRR29 pKa = 11.84SQEE32 pKa = 3.93ILKK35 pKa = 10.46KK36 pKa = 10.79AGINPDD42 pKa = 3.06IRR44 pKa = 11.84VKK46 pKa = 10.9DD47 pKa = 3.64LTDD50 pKa = 3.42EE51 pKa = 4.01QVQAIRR57 pKa = 11.84NAMEE61 pKa = 5.22GYY63 pKa = 8.39TLEE66 pKa = 4.68GDD68 pKa = 3.9LRR70 pKa = 11.84RR71 pKa = 11.84EE72 pKa = 3.55VALNIKK78 pKa = 9.97RR79 pKa = 11.84LTEE82 pKa = 3.76IGCYY86 pKa = 9.29RR87 pKa = 11.84GLRR90 pKa = 11.84HH91 pKa = 6.72RR92 pKa = 11.84RR93 pKa = 11.84GLPVRR98 pKa = 11.84GQRR101 pKa = 11.84TKK103 pKa = 10.45TNARR107 pKa = 11.84TRR109 pKa = 11.84KK110 pKa = 9.44GPRR113 pKa = 11.84KK114 pKa = 9.46LVSKK118 pKa = 10.37KK119 pKa = 10.39KK120 pKa = 10.2KK121 pKa = 9.94

Molecular weight: 13.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1194

0

1194

365637

31

2962

306.2

34.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.577 ± 0.062

1.065 ± 0.03

5.575 ± 0.061

8.256 ± 0.086

3.706 ± 0.047

5.815 ± 0.074

1.18 ± 0.023

9.943 ± 0.092

9.793 ± 0.073

8.316 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.481 ± 0.035

6.616 ± 0.075

2.622 ± 0.034

2.99 ± 0.04

3.379 ± 0.051

5.658 ± 0.057

5.793 ± 0.078

6.247 ± 0.063

0.613 ± 0.021

4.372 ± 0.064
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski