Amino acid dipepetide frequency for Clostridium colicanis DSM 13634

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.6AlaAla: 4.6 ± 0.108
0.749AlaCys: 0.749 ± 0.037
2.588AlaAsp: 2.588 ± 0.056
3.753AlaGlu: 3.753 ± 0.089
2.567AlaPhe: 2.567 ± 0.072
4.088AlaGly: 4.088 ± 0.101
0.897AlaHis: 0.897 ± 0.034
6.026AlaIle: 6.026 ± 0.111
5.038AlaLys: 5.038 ± 0.094
6.31AlaLeu: 6.31 ± 0.102
1.742AlaMet: 1.742 ± 0.05
2.509AlaAsn: 2.509 ± 0.061
1.578AlaPro: 1.578 ± 0.044
1.554AlaGln: 1.554 ± 0.05
2.058AlaArg: 2.058 ± 0.065
3.519AlaSer: 3.519 ± 0.067
2.665AlaThr: 2.665 ± 0.065
4.751AlaVal: 4.751 ± 0.09
0.358AlaTrp: 0.358 ± 0.022
2.215AlaTyr: 2.215 ± 0.055
0.0AlaXaa: 0.0 ± 0.0
Cys
0.592CysAla: 0.592 ± 0.03
0.187CysCys: 0.187 ± 0.018
0.628CysAsp: 0.628 ± 0.032
0.773CysGlu: 0.773 ± 0.033
0.45CysPhe: 0.45 ± 0.027
1.066CysGly: 1.066 ± 0.045
0.218CysHis: 0.218 ± 0.017
1.195CysIle: 1.195 ± 0.045
0.958CysLys: 0.958 ± 0.044
0.848CysLeu: 0.848 ± 0.035
0.296CysMet: 0.296 ± 0.022
0.651CysAsn: 0.651 ± 0.027
0.509CysPro: 0.509 ± 0.023
0.198CysGln: 0.198 ± 0.018
0.435CysArg: 0.435 ± 0.025
0.69CysSer: 0.69 ± 0.028
0.519CysThr: 0.519 ± 0.029
0.579CysVal: 0.579 ± 0.031
0.047CysTrp: 0.047 ± 0.007
0.459CysTyr: 0.459 ± 0.028
0.0CysXaa: 0.0 ± 0.0
Asp
2.919AspAla: 2.919 ± 0.072
0.55AspCys: 0.55 ± 0.029
2.665AspAsp: 2.665 ± 0.068
4.628AspGlu: 4.628 ± 0.089
2.557AspPhe: 2.557 ± 0.056
3.218AspGly: 3.218 ± 0.072
0.559AspHis: 0.559 ± 0.029
6.36AspIle: 6.36 ± 0.101
5.57AspLys: 5.57 ± 0.097
4.743AspLeu: 4.743 ± 0.085
1.706AspMet: 1.706 ± 0.048
3.125AspAsn: 3.125 ± 0.069
1.423AspPro: 1.423 ± 0.049
0.601AspGln: 0.601 ± 0.031
1.924AspArg: 1.924 ± 0.048
2.85AspSer: 2.85 ± 0.06
2.512AspThr: 2.512 ± 0.057
3.453AspVal: 3.453 ± 0.079
0.325AspTrp: 0.325 ± 0.022
2.528AspTyr: 2.528 ± 0.06
0.0AspXaa: 0.0 ± 0.0
Glu
4.653GluAla: 4.653 ± 0.085
0.725GluCys: 0.725 ± 0.031
4.57GluAsp: 4.57 ± 0.1
7.613GluGlu: 7.613 ± 0.135
2.989GluPhe: 2.989 ± 0.071
4.432GluGly: 4.432 ± 0.082
0.946GluHis: 0.946 ± 0.035
7.339GluIle: 7.339 ± 0.13
7.979GluLys: 7.979 ± 0.117
6.79GluLeu: 6.79 ± 0.114
1.965GluMet: 1.965 ± 0.062
5.211GluAsn: 5.211 ± 0.089
1.51GluPro: 1.51 ± 0.049
1.792GluGln: 1.792 ± 0.052
2.865GluArg: 2.865 ± 0.071
3.394GluSer: 3.394 ± 0.068
2.793GluThr: 2.793 ± 0.067
5.258GluVal: 5.258 ± 0.103
0.427GluTrp: 0.427 ± 0.027
3.047GluTyr: 3.047 ± 0.072
0.0GluXaa: 0.0 ± 0.0
Phe
2.289PheAla: 2.289 ± 0.068
0.49PheCys: 0.49 ± 0.027
2.389PheAsp: 2.389 ± 0.057
2.57PheGlu: 2.57 ± 0.052
1.816PhePhe: 1.816 ± 0.058
2.801PheGly: 2.801 ± 0.074
0.59PheHis: 0.59 ± 0.029
4.534PheIle: 4.534 ± 0.099
3.757PheLys: 3.757 ± 0.071
3.796PheLeu: 3.796 ± 0.093
1.143PheMet: 1.143 ± 0.041
2.774PheAsn: 2.774 ± 0.069
1.232PhePro: 1.232 ± 0.042
0.97PheGln: 0.97 ± 0.032
1.334PheArg: 1.334 ± 0.044
2.921PheSer: 2.921 ± 0.064
2.159PheThr: 2.159 ± 0.06
2.521PheVal: 2.521 ± 0.063
0.253PheTrp: 0.253 ± 0.017
1.617PheTyr: 1.617 ± 0.048
0.0PheXaa: 0.0 ± 0.0
Gly
4.328GlyAla: 4.328 ± 0.094
0.907GlyCys: 0.907 ± 0.045
3.268GlyAsp: 3.268 ± 0.067
4.589GlyGlu: 4.589 ± 0.091
3.025GlyPhe: 3.025 ± 0.072
4.545GlyGly: 4.545 ± 0.115
1.052GlyHis: 1.052 ± 0.045
7.226GlyIle: 7.226 ± 0.119
5.898GlyLys: 5.898 ± 0.1
5.422GlyLeu: 5.422 ± 0.1
1.769GlyMet: 1.769 ± 0.053
3.118GlyAsn: 3.118 ± 0.069
1.341GlyPro: 1.341 ± 0.051
1.461GlyGln: 1.461 ± 0.046
2.483GlyArg: 2.483 ± 0.062
3.611GlySer: 3.611 ± 0.071
3.499GlyThr: 3.499 ± 0.078
4.742GlyVal: 4.742 ± 0.078
0.447GlyTrp: 0.447 ± 0.025
2.896GlyTyr: 2.896 ± 0.056
0.0GlyXaa: 0.0 ± 0.0
His
0.729HisAla: 0.729 ± 0.028
0.177HisCys: 0.177 ± 0.018
0.741HisAsp: 0.741 ± 0.032
0.905HisGlu: 0.905 ± 0.036
0.627HisPhe: 0.627 ± 0.032
1.017HisGly: 1.017 ± 0.041
0.302HisHis: 0.302 ± 0.021
1.477HisIle: 1.477 ± 0.048
1.105HisLys: 1.105 ± 0.04
1.12HisLeu: 1.12 ± 0.039
0.402HisMet: 0.402 ± 0.024
0.799HisAsn: 0.799 ± 0.029
0.652HisPro: 0.652 ± 0.031
0.305HisGln: 0.305 ± 0.019
0.563HisArg: 0.563 ± 0.028
0.901HisSer: 0.901 ± 0.036
0.682HisThr: 0.682 ± 0.032
0.74HisVal: 0.74 ± 0.032
0.094HisTrp: 0.094 ± 0.01
0.517HisTyr: 0.517 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
6.173IleAla: 6.173 ± 0.116
1.195IleCys: 1.195 ± 0.045
6.047IleAsp: 6.047 ± 0.091
7.612IleGlu: 7.612 ± 0.104
4.111IlePhe: 4.111 ± 0.089
6.646IleGly: 6.646 ± 0.1
1.368IleHis: 1.368 ± 0.047
10.017IleIle: 10.017 ± 0.146
9.448IleLys: 9.448 ± 0.129
9.417IleLeu: 9.417 ± 0.145
2.479IleMet: 2.479 ± 0.056
6.089IleAsn: 6.089 ± 0.102
3.548IlePro: 3.548 ± 0.079
2.239IleGln: 2.239 ± 0.059
3.329IleArg: 3.329 ± 0.062
6.903IleSer: 6.903 ± 0.103
4.945IleThr: 4.945 ± 0.084
6.274IleVal: 6.274 ± 0.102
0.55IleTrp: 0.55 ± 0.031
3.48IleTyr: 3.48 ± 0.074
0.0IleXaa: 0.0 ± 0.0
Lys
5.204LysAla: 5.204 ± 0.081
0.85LysCys: 0.85 ± 0.041
6.026LysAsp: 6.026 ± 0.108
9.303LysGlu: 9.303 ± 0.142
3.246LysPhe: 3.246 ± 0.074
5.476LysGly: 5.476 ± 0.103
1.137LysHis: 1.137 ± 0.042
8.723LysIle: 8.723 ± 0.112
8.574LysLys: 8.574 ± 0.135
7.718LysLeu: 7.718 ± 0.097
2.358LysMet: 2.358 ± 0.054
6.381LysAsn: 6.381 ± 0.104
2.167LysPro: 2.167 ± 0.057
2.122LysGln: 2.122 ± 0.055
3.347LysArg: 3.347 ± 0.063
5.116LysSer: 5.116 ± 0.079
3.891LysThr: 3.891 ± 0.067
6.31LysVal: 6.31 ± 0.096
0.505LysTrp: 0.505 ± 0.027
4.192LysTyr: 4.192 ± 0.087
0.0LysXaa: 0.0 ± 0.0
Leu
5.275LeuAla: 5.275 ± 0.091
1.016LeuCys: 1.016 ± 0.039
4.968LeuAsp: 4.968 ± 0.096
6.352LeuGlu: 6.352 ± 0.097
3.487LeuPhe: 3.487 ± 0.078
6.248LeuGly: 6.248 ± 0.111
1.124LeuHis: 1.124 ± 0.033
8.285LeuIle: 8.285 ± 0.114
8.873LeuLys: 8.873 ± 0.118
7.515LeuLeu: 7.515 ± 0.123
2.335LeuMet: 2.335 ± 0.064
5.559LeuAsn: 5.559 ± 0.079
2.815LeuPro: 2.815 ± 0.069
2.117LeuGln: 2.117 ± 0.058
3.315LeuArg: 3.315 ± 0.072
6.562LeuSer: 6.562 ± 0.099
4.305LeuThr: 4.305 ± 0.079
5.316LeuVal: 5.316 ± 0.085
0.492LeuTrp: 0.492 ± 0.026
3.201LeuTyr: 3.201 ± 0.073
0.0LeuXaa: 0.0 ± 0.0
Met
1.885MetAla: 1.885 ± 0.062
0.236MetCys: 0.236 ± 0.018
1.548MetAsp: 1.548 ± 0.043
2.004MetGlu: 2.004 ± 0.05
1.023MetPhe: 1.023 ± 0.038
1.892MetGly: 1.892 ± 0.057
0.375MetHis: 0.375 ± 0.021
2.37MetIle: 2.37 ± 0.054
2.529MetLys: 2.529 ± 0.059
2.407MetLeu: 2.407 ± 0.058
0.637MetMet: 0.637 ± 0.032
1.618MetAsn: 1.618 ± 0.046
0.954MetPro: 0.954 ± 0.04
0.635MetGln: 0.635 ± 0.029
0.934MetArg: 0.934 ± 0.035
1.651MetSer: 1.651 ± 0.046
1.194MetThr: 1.194 ± 0.044
1.601MetVal: 1.601 ± 0.05
0.143MetTrp: 0.143 ± 0.016
0.914MetTyr: 0.914 ± 0.037
0.0MetXaa: 0.0 ± 0.0
Asn
3.067AsnAla: 3.067 ± 0.069
0.635AsnCys: 0.635 ± 0.029
2.669AsnAsp: 2.669 ± 0.071
3.908AsnGlu: 3.908 ± 0.075
2.404AsnPhe: 2.404 ± 0.055
3.421AsnGly: 3.421 ± 0.071
0.741AsnHis: 0.741 ± 0.033
7.236AsnIle: 7.236 ± 0.11
6.059AsnLys: 6.059 ± 0.102
5.361AsnLeu: 5.361 ± 0.098
1.687AsnMet: 1.687 ± 0.047
4.05AsnAsn: 4.05 ± 0.099
2.153AsnPro: 2.153 ± 0.065
1.225AsnGln: 1.225 ± 0.045
2.114AsnArg: 2.114 ± 0.056
3.718AsnSer: 3.718 ± 0.073
2.653AsnThr: 2.653 ± 0.058
3.591AsnVal: 3.591 ± 0.076
0.399AsnTrp: 0.399 ± 0.025
2.443AsnTyr: 2.443 ± 0.06
0.0AsnXaa: 0.0 ± 0.0
Pro
1.54ProAla: 1.54 ± 0.052
0.361ProCys: 0.361 ± 0.019
1.504ProAsp: 1.504 ± 0.048
2.421ProGlu: 2.421 ± 0.061
1.364ProPhe: 1.364 ± 0.048
1.873ProGly: 1.873 ± 0.064
0.542ProHis: 0.542 ± 0.027
2.964ProIle: 2.964 ± 0.068
2.421ProLys: 2.421 ± 0.065
2.49ProLeu: 2.49 ± 0.055
0.74ProMet: 0.74 ± 0.032
1.636ProAsn: 1.636 ± 0.049
0.722ProPro: 0.722 ± 0.034
0.808ProGln: 0.808 ± 0.035
0.919ProArg: 0.919 ± 0.036
1.783ProSer: 1.783 ± 0.06
1.494ProThr: 1.494 ± 0.047
2.194ProVal: 2.194 ± 0.06
0.205ProTrp: 0.205 ± 0.017
1.381ProTyr: 1.381 ± 0.044
0.0ProXaa: 0.0 ± 0.0
Gln
1.413GlnAla: 1.413 ± 0.055
0.272GlnCys: 0.272 ± 0.022
1.179GlnAsp: 1.179 ± 0.037
1.664GlnGlu: 1.664 ± 0.049
0.903GlnPhe: 0.903 ± 0.039
1.512GlnGly: 1.512 ± 0.043
0.322GlnHis: 0.322 ± 0.02
2.176GlnIle: 2.176 ± 0.048
1.971GlnLys: 1.971 ± 0.055
1.889GlnLeu: 1.889 ± 0.058
0.687GlnMet: 0.687 ± 0.033
1.431GlnAsn: 1.431 ± 0.04
0.592GlnPro: 0.592 ± 0.031
0.68GlnGln: 0.68 ± 0.037
1.015GlnArg: 1.015 ± 0.041
1.19GlnSer: 1.19 ± 0.043
0.966GlnThr: 0.966 ± 0.038
1.555GlnVal: 1.555 ± 0.045
0.185GlnTrp: 0.185 ± 0.016
1.027GlnTyr: 1.027 ± 0.038
0.0GlnXaa: 0.0 ± 0.0
Arg
2.091ArgAla: 2.091 ± 0.058
0.441ArgCys: 0.441 ± 0.024
1.841ArgAsp: 1.841 ± 0.054
3.35ArgGlu: 3.35 ± 0.083
1.562ArgPhe: 1.562 ± 0.052
2.226ArgGly: 2.226 ± 0.061
0.501ArgHis: 0.501 ± 0.025
3.638ArgIle: 3.638 ± 0.07
3.317ArgLys: 3.317 ± 0.071
3.106ArgLeu: 3.106 ± 0.065
1.0ArgMet: 1.0 ± 0.037
2.045ArgAsn: 2.045 ± 0.055
0.989ArgPro: 0.989 ± 0.039
0.953ArgGln: 0.953 ± 0.037
1.691ArgArg: 1.691 ± 0.057
1.645ArgSer: 1.645 ± 0.045
1.617ArgThr: 1.617 ± 0.046
2.35ArgVal: 2.35 ± 0.063
0.271ArgTrp: 0.271 ± 0.021
1.462ArgTyr: 1.462 ± 0.043
0.0ArgXaa: 0.0 ± 0.0
Ser
3.251SerAla: 3.251 ± 0.076
0.632SerCys: 0.632 ± 0.03
2.805SerAsp: 2.805 ± 0.069
3.909SerGlu: 3.909 ± 0.08
2.848SerPhe: 2.848 ± 0.057
4.149SerGly: 4.149 ± 0.076
0.907SerHis: 0.907 ± 0.035
6.465SerIle: 6.465 ± 0.101
5.716SerLys: 5.716 ± 0.1
5.737SerLeu: 5.737 ± 0.104
1.652SerMet: 1.652 ± 0.053
3.505SerAsn: 3.505 ± 0.077
1.725SerPro: 1.725 ± 0.052
1.533SerGln: 1.533 ± 0.047
2.234SerArg: 2.234 ± 0.054
4.255SerSer: 4.255 ± 0.09
2.972SerThr: 2.972 ± 0.059
3.692SerVal: 3.692 ± 0.068
0.376SerTrp: 0.376 ± 0.023
2.425SerTyr: 2.425 ± 0.063
0.0SerXaa: 0.0 ± 0.0
Thr
3.098ThrAla: 3.098 ± 0.082
0.438ThrCys: 0.438 ± 0.026
2.186ThrAsp: 2.186 ± 0.053
2.842ThrGlu: 2.842 ± 0.07
2.012ThrPhe: 2.012 ± 0.059
3.629ThrGly: 3.629 ± 0.075
0.714ThrHis: 0.714 ± 0.032
4.813ThrIle: 4.813 ± 0.083
3.471ThrLys: 3.471 ± 0.061
4.728ThrLeu: 4.728 ± 0.078
1.091ThrMet: 1.091 ± 0.037
2.402ThrAsn: 2.402 ± 0.053
1.85ThrPro: 1.85 ± 0.043
1.09ThrGln: 1.09 ± 0.037
1.61ThrArg: 1.61 ± 0.047
2.913ThrSer: 2.913 ± 0.062
2.458ThrThr: 2.458 ± 0.062
3.397ThrVal: 3.397 ± 0.07
0.286ThrTrp: 0.286 ± 0.02
1.753ThrTyr: 1.753 ± 0.054
0.0ThrXaa: 0.0 ± 0.0
Val
3.968ValAla: 3.968 ± 0.087
0.837ValCys: 0.837 ± 0.034
3.879ValAsp: 3.879 ± 0.077
4.793ValGlu: 4.793 ± 0.094
2.866ValPhe: 2.866 ± 0.07
4.337ValGly: 4.337 ± 0.087
0.896ValHis: 0.896 ± 0.035
6.322ValIle: 6.322 ± 0.092
5.74ValLys: 5.74 ± 0.099
5.965ValLeu: 5.965 ± 0.094
1.674ValMet: 1.674 ± 0.049
3.519ValAsn: 3.519 ± 0.063
2.208ValPro: 2.208 ± 0.054
1.512ValGln: 1.512 ± 0.041
2.15ValArg: 2.15 ± 0.061
4.302ValSer: 4.302 ± 0.079
3.286ValThr: 3.286 ± 0.075
4.635ValVal: 4.635 ± 0.089
0.38ValTrp: 0.38 ± 0.025
2.45ValTyr: 2.45 ± 0.063
0.0ValXaa: 0.0 ± 0.0
Trp
0.377TrpAla: 0.377 ± 0.025
0.089TrpCys: 0.089 ± 0.01
0.315TrpAsp: 0.315 ± 0.02
0.418TrpGlu: 0.418 ± 0.023
0.291TrpPhe: 0.291 ± 0.019
0.426TrpGly: 0.426 ± 0.025
0.12TrpHis: 0.12 ± 0.013
0.597TrpIle: 0.597 ± 0.032
0.507TrpLys: 0.507 ± 0.024
0.508TrpLeu: 0.508 ± 0.03
0.182TrpMet: 0.182 ± 0.015
0.399TrpAsn: 0.399 ± 0.023
0.14TrpPro: 0.14 ± 0.014
0.187TrpGln: 0.187 ± 0.017
0.259TrpArg: 0.259 ± 0.017
0.34TrpSer: 0.34 ± 0.025
0.251TrpThr: 0.251 ± 0.017
0.369TrpVal: 0.369 ± 0.023
0.061TrpTrp: 0.061 ± 0.01
0.22TrpTyr: 0.22 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.114TyrAla: 2.114 ± 0.05
0.513TyrCys: 0.513 ± 0.028
2.398TyrAsp: 2.398 ± 0.055
2.892TyrGlu: 2.892 ± 0.077
1.81TyrPhe: 1.81 ± 0.045
2.626TyrGly: 2.626 ± 0.071
0.543TyrHis: 0.543 ± 0.025
4.106TyrIle: 4.106 ± 0.085
3.716TyrLys: 3.716 ± 0.083
3.29TyrLeu: 3.29 ± 0.078
1.001TyrMet: 1.001 ± 0.04
2.711TyrAsn: 2.711 ± 0.077
1.254TyrPro: 1.254 ± 0.039
0.647TyrGln: 0.647 ± 0.029
1.516TyrArg: 1.516 ± 0.047
2.525TyrSer: 2.525 ± 0.059
1.919TyrThr: 1.919 ± 0.056
2.394TyrVal: 2.394 ± 0.058
0.251TyrTrp: 0.251 ± 0.017
1.775TyrTyr: 1.775 ± 0.051
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2406 proteins (742156 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski