Chaetoceros protobacilladnavirus 1
Average proteome isoelectric point is 7.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q2L6L9|Q2L6L9_9VIRU Uncharacterized protein OS=Chaetoceros protobacilladnavirus 1 OX=2170140 PE=4 SV=1
MM1 pKa = 7.71 RR2 pKa = 11.84 NFEE5 pKa = 4.72 RR6 pKa = 11.84 IMNQVEE12 pKa = 4.33 DD13 pKa = 3.81 EE14 pKa = 4.57 HH15 pKa = 7.58 NLPNHH20 pKa = 6.28 VYY22 pKa = 10.88 ANLMDD27 pKa = 5.77 DD28 pKa = 4.21 SPACIATMASDD39 pKa = 3.93 RR40 pKa = 11.84 QWAGAEE46 pKa = 4.06 LKK48 pKa = 10.5 EE49 pKa = 4.42 IAGKK53 pKa = 10.12 HH54 pKa = 4.48 YY55 pKa = 10.1 FYY57 pKa = 11.33 AFNTYY62 pKa = 10.65 YY63 pKa = 10.83 DD64 pKa = 3.61 IDD66 pKa = 3.75 QLTNRR71 pKa = 11.84 AEE73 pKa = 4.32 RR74 pKa = 11.84 YY75 pKa = 9.39 FLEE78 pKa = 4.44 QKK80 pKa = 6.94 WTLMMYY86 pKa = 10.64 NEE88 pKa = 4.2 FTMYY92 pKa = 9.94 MDD94 pKa = 4.65 MLICGYY100 pKa = 10.09 KK101 pKa = 10.28 FNPHH105 pKa = 4.5 YY106 pKa = 8.97 TAKK109 pKa = 10.72 LFWAPTLQRR118 pKa = 11.84 CVDD121 pKa = 3.7 APIIDD126 pKa = 3.03 IWYY129 pKa = 8.85 QFIYY133 pKa = 9.51 PDD135 pKa = 3.52 EE136 pKa = 4.01 VHH138 pKa = 6.18 YY139 pKa = 11.31 VIDD142 pKa = 3.85 LTSDD146 pKa = 3.36 DD147 pKa = 4.28 GADD150 pKa = 3.35 VPFDD154 pKa = 3.69 TDD156 pKa = 3.9 SIATFDD162 pKa = 3.63 VVGPIDD168 pKa = 4.94 NDD170 pKa = 3.44 ILDD173 pKa = 3.78 VWTTPTVPAIIDD185 pKa = 3.72 VPNLRR190 pKa = 11.84 IDD192 pKa = 3.66 LRR194 pKa = 11.84 DD195 pKa = 3.12 GHH197 pKa = 6.49 EE198 pKa = 5.03 IIDD201 pKa = 4.01 LTNDD205 pKa = 3.6 IISS208 pKa = 3.49
Molecular weight: 24.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.862
IPC2_protein 4.088
IPC_protein 4.101
Toseland 3.872
ProMoST 4.266
Dawson 4.088
Bjellqvist 4.24
Wikipedia 4.037
Rodwell 3.923
Grimsley 3.783
Solomon 4.088
Lehninger 4.05
Nozaki 4.202
DTASelect 4.469
Thurlkill 3.923
EMBOSS 4.05
Sillero 4.215
Patrickios 0.998
IPC_peptide 4.088
IPC2_peptide 4.202
IPC2.peptide.svr19 4.093
Protein with the highest isoelectric point:
>tr|Q2L6L7|Q2L6L7_9VIRU Uncharacterized protein OS=Chaetoceros protobacilladnavirus 1 OX=2170140 PE=4 SV=1
MM1 pKa = 7.75 RR2 pKa = 11.84 AITPKK7 pKa = 10.34 SSYY10 pKa = 10.99 APAVGVKK17 pKa = 8.62 NTMQGAVPLTQEE29 pKa = 4.54 EE30 pKa = 4.68 PSPMLSSSTVPVVLVRR46 pKa = 11.84 PRR48 pKa = 11.84 LPTIGTPVRR57 pKa = 11.84 VKK59 pKa = 9.89 PRR61 pKa = 11.84 KK62 pKa = 8.88 SAIIVEE68 pKa = 4.54 TLTMAISGEE77 pKa = 4.12 LADD80 pKa = 5.07 RR81 pKa = 11.84 PTMGKK86 pKa = 10.17 GLSTSRR92 pKa = 11.84 NLQAKK97 pKa = 9.83 RR98 pKa = 11.84 PLADD102 pKa = 3.0
Molecular weight: 10.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.298
IPC2_protein 9.823
IPC_protein 10.789
Toseland 11.155
ProMoST 11.125
Dawson 11.199
Bjellqvist 10.965
Wikipedia 11.477
Rodwell 11.374
Grimsley 11.228
Solomon 11.447
Lehninger 11.403
Nozaki 11.14
DTASelect 10.965
Thurlkill 11.14
EMBOSS 11.594
Sillero 11.14
Patrickios 11.14
IPC_peptide 11.462
IPC2_peptide 9.97
IPC2.peptide.svr19 8.772
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1799
102
573
299.8
33.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.115 ± 0.837
1.334 ± 0.489
6.448 ± 1.024
5.392 ± 0.57
4.169 ± 0.551
7.449 ± 0.797
2.446 ± 0.656
5.725 ± 0.658
5.67 ± 0.902
7.059 ± 0.588
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.223 ± 0.677
4.558 ± 0.649
5.892 ± 0.753
3.835 ± 0.432
6.392 ± 0.439
6.559 ± 0.804
6.893 ± 0.783
5.503 ± 0.95
2.001 ± 0.502
3.335 ± 0.709
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here