Pseudoxanthomonas wuyuanensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Pseudoxanthomonas

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3989 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A286D655|A0A286D655_9GAMM Transcriptional regulator AraC family OS=Pseudoxanthomonas wuyuanensis OX=1073196 GN=SAMN06296416_10395 PE=4 SV=1
SS1 pKa = 6.03VGSVGAEE8 pKa = 3.35RR9 pKa = 11.84TITNVAAGRR18 pKa = 11.84ISGSSTDD25 pKa = 4.69AINGSQLFATNQAIAAIAASTPVHH49 pKa = 5.88YY50 pKa = 10.42FSVNDD55 pKa = 4.71GGTQQANYY63 pKa = 10.08LNDD66 pKa = 3.61GATAPFAIAIGSNATATGSYY86 pKa = 7.95GTAVGEE92 pKa = 4.24GATAIGDD99 pKa = 3.48WATAYY104 pKa = 10.73GNDD107 pKa = 3.68ANAGTTTATAIGAGTSATATFAFGAGFQATASGVSSTAVGLHH149 pKa = 6.25ANASGEE155 pKa = 4.16SGIAVGTDD163 pKa = 2.9AASTGTYY170 pKa = 10.17AIAIGRR176 pKa = 11.84NASATGSHH184 pKa = 5.36GTAVGEE190 pKa = 4.2GATAIGDD197 pKa = 3.48WATAYY202 pKa = 10.75GNGANASTTTATAIGGGTSATATFAFGAGYY232 pKa = 9.67QATAGGIASTAVGLHH247 pKa = 6.15ANATGEE253 pKa = 4.07SSIAIGTDD261 pKa = 3.03AVADD265 pKa = 3.7SDD267 pKa = 3.91EE268 pKa = 4.14AVAIGRR274 pKa = 11.84GSVATGGRR282 pKa = 11.84AVAIGSGNVANGDD295 pKa = 3.61GAVAIGDD302 pKa = 4.13PNTATGNGAIASGLDD317 pKa = 3.17NTATGNGSVAMGNTNMVGGGGQAVSTPGTAAQGAVGIGYY356 pKa = 9.49QNTVIGQGSVALGSTSSALAAGAVAFGDD384 pKa = 3.51TAVANNAGDD393 pKa = 3.63VALGSGSVTDD403 pKa = 3.76TAVATASTVIDD414 pKa = 3.59GVTYY418 pKa = 10.85NFAGTTPTSTVSVGAVGSEE437 pKa = 3.58RR438 pKa = 11.84TITNVAAGRR447 pKa = 11.84ISDD450 pKa = 3.88TSTDD454 pKa = 4.24AINGSQLYY462 pKa = 8.24ATNQAIEE469 pKa = 4.26AVATTASAGWNLSAQGANATNVAPDD494 pKa = 3.44EE495 pKa = 4.33TVDD498 pKa = 5.09LNNSDD503 pKa = 3.65GNIVVSKK510 pKa = 9.28TATDD514 pKa = 3.82DD515 pKa = 4.84DD516 pKa = 4.4VTFDD520 pKa = 4.12LAADD524 pKa = 3.52ITVDD528 pKa = 3.32SVTAGGTVLNTNGLTIAGGPSVTTTGINAGGLVISNVAPGVAGTDD573 pKa = 3.37AVNVDD578 pKa = 3.45QLTSVSTAGLNFTGNDD594 pKa = 3.08NTAGDD599 pKa = 3.62VHH601 pKa = 7.43RR602 pKa = 11.84DD603 pKa = 3.27LGQTLAIQGGASTAGTYY620 pKa = 10.39SGGNLRR626 pKa = 11.84TVTDD630 pKa = 4.13PVTGAIQLQMADD642 pKa = 3.75SPVFGATTVNAGGTGIISGVTAGVADD668 pKa = 4.0TDD670 pKa = 4.0AVNVSQLNAIGDD682 pKa = 3.75IANAGWNISAQGANATNVAPDD703 pKa = 3.44EE704 pKa = 4.33TVDD707 pKa = 5.09LNNSDD712 pKa = 3.65GNIVVSKK719 pKa = 9.28TATDD723 pKa = 3.82DD724 pKa = 4.84DD725 pKa = 4.4VTFDD729 pKa = 4.12LAADD733 pKa = 3.52ITVDD737 pKa = 3.78SVTAGNSVLNTSGLTVDD754 pKa = 5.19DD755 pKa = 4.84GVNSTSVGSTGLVITGGPSVTTTGIDD781 pKa = 3.07AGSLVISNVAPGVAGTDD798 pKa = 3.37AVNVDD803 pKa = 3.45QLTSVSTAGLNFTGNDD819 pKa = 3.08NTAGDD824 pKa = 3.62VHH826 pKa = 7.43RR827 pKa = 11.84DD828 pKa = 3.27LGQTLAIRR836 pKa = 11.84GGASTAGTYY845 pKa = 10.39SGGNLRR851 pKa = 11.84TVTDD855 pKa = 4.01PATGAIQLQMADD867 pKa = 3.56SPVFGGTTVNAGGTGIISGVTAGVADD893 pKa = 4.0TDD895 pKa = 4.0AVNVSQLNAIGDD907 pKa = 3.74IATAGWNLSAQGANATNVAPDD928 pKa = 3.44EE929 pKa = 4.33TVDD932 pKa = 5.09LNNSDD937 pKa = 3.65GNIVVSKK944 pKa = 10.71NATDD948 pKa = 4.25DD949 pKa = 4.77DD950 pKa = 4.54VTFDD954 pKa = 4.04LADD957 pKa = 5.61DD958 pKa = 3.76ITVDD962 pKa = 3.41SVTAGGTVLNSNGLTIAGGPSVTTTGINAGGLVISNVAPGVAGTDD1007 pKa = 3.37AVNVDD1012 pKa = 3.25QLTAVGNIANAGWNITDD1029 pKa = 3.84GTNSGNIAPNEE1040 pKa = 4.2TLTVAAGSNATVVYY1054 pKa = 10.29DD1055 pKa = 4.38DD1056 pKa = 3.78STGTMTVGVVADD1068 pKa = 4.12PSFNSITVGDD1078 pKa = 3.89TVINDD1083 pKa = 3.41GSISFTSGGPSITNIGIDD1101 pKa = 3.75AGNTTITNVAAAVNNTDD1118 pKa = 3.52AVNLEE1123 pKa = 4.05QVTNLVEE1130 pKa = 4.49GATTRR1135 pKa = 11.84YY1136 pKa = 10.47YY1137 pKa = 11.01SVNDD1141 pKa = 3.38NGVQGGNYY1149 pKa = 9.72NNDD1152 pKa = 3.02GATGINAMAAGVGASSAGAQGVALGNNASVITDD1185 pKa = 3.7GGVALGSGSLSDD1197 pKa = 4.39RR1198 pKa = 11.84VVAPQSGGIIAGTGLIPYY1216 pKa = 7.62NTTDD1220 pKa = 3.11KK1221 pKa = 10.43TLLGAVSVGTDD1232 pKa = 2.96TSYY1235 pKa = 11.36RR1236 pKa = 11.84QITNVADD1243 pKa = 3.7GTEE1246 pKa = 4.21DD1247 pKa = 3.21QDD1249 pKa = 5.01AVTLRR1254 pKa = 11.84QLRR1257 pKa = 11.84NGLSSFAVTPTMYY1270 pKa = 10.49FHH1272 pKa = 7.45ANSTAPDD1279 pKa = 3.48SLAIGAEE1286 pKa = 4.33SVAIGPNTTVNGDD1299 pKa = 3.18NGIGMGNGALVQQTAPGGIAIGQDD1323 pKa = 3.5TIVNFADD1330 pKa = 4.6GVALGTNAVTNGIQAMALGAGATANEE1356 pKa = 4.54PGSVALGAGSVTAVAVATPSTTIDD1380 pKa = 3.1GVTYY1384 pKa = 10.32NYY1386 pKa = 10.9AGTAPTSTVSVGAAGTEE1403 pKa = 3.85RR1404 pKa = 11.84TITHH1408 pKa = 7.08LAAGRR1413 pKa = 11.84ISADD1417 pKa = 2.96STDD1420 pKa = 3.76AVNGSQLFAAHH1431 pKa = 6.1QSIEE1435 pKa = 4.1NLSTRR1440 pKa = 11.84IDD1442 pKa = 3.46ASATHH1447 pKa = 6.45YY1448 pKa = 11.15YY1449 pKa = 10.24SVNDD1453 pKa = 3.53NGVVGGNYY1461 pKa = 10.14NNDD1464 pKa = 3.12GATGINSLATGVDD1477 pKa = 3.7SAASGDD1483 pKa = 3.49ASVAIGFGATAADD1496 pKa = 3.99ANSVALGSNATTAAAVGTASIVIGGTTYY1524 pKa = 11.23NFAGTAPVGTVSVGDD1539 pKa = 3.55VGAEE1543 pKa = 3.55RR1544 pKa = 11.84TITNVAAGRR1553 pKa = 11.84IGADD1557 pKa = 2.83STDD1560 pKa = 3.41AVNGSQLHH1568 pKa = 5.69ATNQAIANLSNTVNASTAHH1587 pKa = 5.95YY1588 pKa = 10.49YY1589 pKa = 10.36SVNDD1593 pKa = 3.9GGVQGGNYY1601 pKa = 9.87NNDD1604 pKa = 3.26GASGVNAVASGVNAAASGQGSVAVGANSNAAGAGSVAVGSGSQSTVAGGVAIGADD1659 pKa = 3.88SVSSTAAGVAGYY1671 pKa = 10.28VPAGATAGQAAAIAATTSTSGAVSVGDD1698 pKa = 3.99ASSGLYY1704 pKa = 9.79RR1705 pKa = 11.84QITGVAAGTAASDD1718 pKa = 3.81AVNVAQLQAVQNQLNATATHH1738 pKa = 6.57YY1739 pKa = 11.18YY1740 pKa = 9.73SVNDD1744 pKa = 3.92GGVQGGNYY1752 pKa = 9.8NNDD1755 pKa = 3.03GATGVNSMAGGVNSTASGSNSVALGANSNASAANSAALGSGSQATHH1801 pKa = 5.76VNSVAIGNGSATTVGAQTGYY1821 pKa = 9.17NAAYY1825 pKa = 9.9VGSSNSTGEE1834 pKa = 3.89VNMGGRR1840 pKa = 11.84TITGVAPGIAGTDD1853 pKa = 3.24AVNVNQLNAGVNHH1866 pKa = 7.21AVNQANQYY1874 pKa = 9.04TDD1876 pKa = 2.92MRR1878 pKa = 11.84LAEE1881 pKa = 4.84LDD1883 pKa = 3.43NDD1885 pKa = 3.34VWTMEE1890 pKa = 3.81RR1891 pKa = 11.84GYY1893 pKa = 10.92RR1894 pKa = 11.84GATSSAMAMAGLPQAYY1910 pKa = 10.01LPGKK1914 pKa = 10.56SMLAVGFGGYY1924 pKa = 8.88QGEE1927 pKa = 4.42YY1928 pKa = 10.18GMAVGLSGITDD1939 pKa = 3.41NGRR1942 pKa = 11.84WVYY1945 pKa = 10.26KK1946 pKa = 10.62ASASGNTSRR1955 pKa = 11.84DD1956 pKa = 2.75WGFSVGAGIQWW1967 pKa = 3.17

Molecular weight: 188.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A286DBA4|A0A286DBA4_9GAMM Chemotaxis protein MotA OS=Pseudoxanthomonas wuyuanensis OX=1073196 GN=SAMN06296416_10886 PE=3 SV=1
MM1 pKa = 7.37LNSVAVGRR9 pKa = 11.84RR10 pKa = 11.84LALRR14 pKa = 11.84AAAYY18 pKa = 9.8QLLAVGLVAALFLLRR33 pKa = 11.84GLPQALAAALGGLAMLAGGLVAARR57 pKa = 11.84LALGGGVIPAGAAMLRR73 pKa = 11.84LLAGVVLKK81 pKa = 9.97WAVVFAVLLVGLGAWKK97 pKa = 10.32LPPLPLMAGLLTGLAAQVLAAARR120 pKa = 11.84RR121 pKa = 11.84QQ122 pKa = 3.35

Molecular weight: 12.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3989

0

3989

1357910

29

4271

340.4

37.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.97 ± 0.061

0.804 ± 0.013

5.756 ± 0.03

5.315 ± 0.035

3.42 ± 0.02

8.631 ± 0.053

2.115 ± 0.022

4.246 ± 0.028

2.669 ± 0.032

10.79 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.117 ± 0.017

2.629 ± 0.035

5.381 ± 0.031

4.032 ± 0.024

7.609 ± 0.047

5.483 ± 0.033

4.86 ± 0.05

7.092 ± 0.031

1.587 ± 0.019

2.493 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski