Amino acid dipepetide frequency for Pseudomonas phage uligo

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.906AlaAla: 9.906 ± 1.089
0.404AlaCys: 0.404 ± 0.225
6.065AlaAsp: 6.065 ± 0.545
6.806AlaGlu: 6.806 ± 1.024
2.965AlaPhe: 2.965 ± 0.495
6.671AlaGly: 6.671 ± 0.779
2.089AlaHis: 2.089 ± 0.333
4.515AlaIle: 4.515 ± 0.387
5.189AlaLys: 5.189 ± 0.613
7.278AlaLeu: 7.278 ± 0.874
3.032AlaMet: 3.032 ± 0.568
4.582AlaAsn: 4.582 ± 0.508
3.976AlaPro: 3.976 ± 0.631
4.582AlaGln: 4.582 ± 0.721
5.189AlaArg: 5.189 ± 0.644
3.774AlaSer: 3.774 ± 0.537
5.323AlaThr: 5.323 ± 0.551
6.334AlaVal: 6.334 ± 0.608
0.943AlaTrp: 0.943 ± 0.282
3.235AlaTyr: 3.235 ± 0.529
0.0AlaXaa: 0.0 ± 0.0
Cys
0.404CysAla: 0.404 ± 0.202
0.135CysCys: 0.135 ± 0.095
0.674CysAsp: 0.674 ± 0.208
0.135CysGlu: 0.135 ± 0.115
0.404CysPhe: 0.404 ± 0.159
0.741CysGly: 0.741 ± 0.264
0.067CysHis: 0.067 ± 0.07
0.809CysIle: 0.809 ± 0.3
0.539CysLys: 0.539 ± 0.193
0.876CysLeu: 0.876 ± 0.22
0.27CysMet: 0.27 ± 0.125
0.741CysAsn: 0.741 ± 0.253
0.539CysPro: 0.539 ± 0.2
0.27CysGln: 0.27 ± 0.122
0.539CysArg: 0.539 ± 0.187
0.337CysSer: 0.337 ± 0.157
0.606CysThr: 0.606 ± 0.2
0.539CysVal: 0.539 ± 0.201
0.0CysTrp: 0.0 ± 0.0
0.27CysTyr: 0.27 ± 0.134
0.0CysXaa: 0.0 ± 0.0
Asp
5.526AspAla: 5.526 ± 0.697
0.337AspCys: 0.337 ± 0.177
3.571AspAsp: 3.571 ± 0.471
4.245AspGlu: 4.245 ± 0.623
2.763AspPhe: 2.763 ± 0.484
4.852AspGly: 4.852 ± 0.82
1.213AspHis: 1.213 ± 0.273
2.898AspIle: 2.898 ± 0.424
4.111AspLys: 4.111 ± 0.666
4.717AspLeu: 4.717 ± 0.541
1.752AspMet: 1.752 ± 0.302
2.426AspAsn: 2.426 ± 0.432
2.965AspPro: 2.965 ± 0.394
2.291AspGln: 2.291 ± 0.336
3.504AspArg: 3.504 ± 0.598
3.639AspSer: 3.639 ± 0.369
4.245AspThr: 4.245 ± 0.515
3.302AspVal: 3.302 ± 0.52
1.011AspTrp: 1.011 ± 0.259
2.291AspTyr: 2.291 ± 0.375
0.0AspXaa: 0.0 ± 0.0
Glu
7.075GluAla: 7.075 ± 0.942
0.472GluCys: 0.472 ± 0.19
3.706GluAsp: 3.706 ± 0.473
4.515GluGlu: 4.515 ± 0.749
2.358GluPhe: 2.358 ± 0.411
4.784GluGly: 4.784 ± 0.566
1.685GluHis: 1.685 ± 0.286
1.954GluIle: 1.954 ± 0.338
3.774GluLys: 3.774 ± 0.468
6.604GluLeu: 6.604 ± 0.689
1.954GluMet: 1.954 ± 0.341
1.685GluAsn: 1.685 ± 0.306
2.224GluPro: 2.224 ± 0.32
2.898GluGln: 2.898 ± 0.47
3.369GluArg: 3.369 ± 0.544
2.898GluSer: 2.898 ± 0.49
3.235GluThr: 3.235 ± 0.428
4.515GluVal: 4.515 ± 0.536
1.28GluTrp: 1.28 ± 0.272
2.156GluTyr: 2.156 ± 0.512
0.0GluXaa: 0.0 ± 0.0
Phe
3.1PheAla: 3.1 ± 0.554
0.202PheCys: 0.202 ± 0.123
3.235PheAsp: 3.235 ± 0.419
1.617PheGlu: 1.617 ± 0.298
1.617PhePhe: 1.617 ± 0.428
2.426PheGly: 2.426 ± 0.421
0.943PheHis: 0.943 ± 0.249
1.954PheIle: 1.954 ± 0.322
1.819PheLys: 1.819 ± 0.345
2.898PheLeu: 2.898 ± 0.403
1.011PheMet: 1.011 ± 0.295
1.887PheAsn: 1.887 ± 0.496
2.224PhePro: 2.224 ± 0.396
1.348PheGln: 1.348 ± 0.531
2.561PheArg: 2.561 ± 0.38
2.426PheSer: 2.426 ± 0.461
2.089PheThr: 2.089 ± 0.457
3.167PheVal: 3.167 ± 0.661
0.606PheTrp: 0.606 ± 0.186
1.011PheTyr: 1.011 ± 0.304
0.0PheXaa: 0.0 ± 0.0
Gly
6.402GlyAla: 6.402 ± 0.626
0.674GlyCys: 0.674 ± 0.271
5.323GlyAsp: 5.323 ± 0.79
4.65GlyGlu: 4.65 ± 0.727
2.83GlyPhe: 2.83 ± 0.537
5.997GlyGly: 5.997 ± 0.655
2.022GlyHis: 2.022 ± 0.555
3.437GlyIle: 3.437 ± 0.517
6.132GlyLys: 6.132 ± 0.64
5.323GlyLeu: 5.323 ± 0.491
2.224GlyMet: 2.224 ± 0.343
3.437GlyAsn: 3.437 ± 0.534
1.685GlyPro: 1.685 ± 0.28
3.437GlyGln: 3.437 ± 0.544
3.908GlyArg: 3.908 ± 0.563
4.38GlySer: 4.38 ± 0.377
4.852GlyThr: 4.852 ± 0.602
5.593GlyVal: 5.593 ± 0.453
1.685GlyTrp: 1.685 ± 0.294
3.032GlyTyr: 3.032 ± 0.494
0.0GlyXaa: 0.0 ± 0.0
His
1.482HisAla: 1.482 ± 0.34
0.202HisCys: 0.202 ± 0.087
1.819HisAsp: 1.819 ± 0.45
1.011HisGlu: 1.011 ± 0.3
1.146HisPhe: 1.146 ± 0.229
1.685HisGly: 1.685 ± 0.447
0.404HisHis: 0.404 ± 0.184
0.809HisIle: 0.809 ± 0.166
1.617HisLys: 1.617 ± 0.421
2.763HisLeu: 2.763 ± 0.515
0.606HisMet: 0.606 ± 0.187
0.741HisAsn: 0.741 ± 0.239
1.146HisPro: 1.146 ± 0.31
0.943HisGln: 0.943 ± 0.25
1.213HisArg: 1.213 ± 0.302
1.415HisSer: 1.415 ± 0.321
1.011HisThr: 1.011 ± 0.224
1.213HisVal: 1.213 ± 0.3
0.539HisTrp: 0.539 ± 0.188
1.011HisTyr: 1.011 ± 0.202
0.0HisXaa: 0.0 ± 0.0
Ile
5.728IleAla: 5.728 ± 0.568
0.135IleCys: 0.135 ± 0.099
3.369IleAsp: 3.369 ± 0.325
3.504IleGlu: 3.504 ± 0.41
1.55IlePhe: 1.55 ± 0.34
4.111IleGly: 4.111 ± 0.472
1.146IleHis: 1.146 ± 0.239
2.291IleIle: 2.291 ± 0.541
2.898IleLys: 2.898 ± 0.357
2.628IleLeu: 2.628 ± 0.476
0.606IleMet: 0.606 ± 0.155
2.089IleAsn: 2.089 ± 0.527
2.493IlePro: 2.493 ± 0.478
2.628IleGln: 2.628 ± 0.593
3.302IleArg: 3.302 ± 0.396
2.291IleSer: 2.291 ± 0.368
3.032IleThr: 3.032 ± 0.362
2.561IleVal: 2.561 ± 0.411
0.741IleTrp: 0.741 ± 0.337
0.943IleTyr: 0.943 ± 0.267
0.0IleXaa: 0.0 ± 0.0
Lys
5.66LysAla: 5.66 ± 0.912
0.809LysCys: 0.809 ± 0.204
3.908LysAsp: 3.908 ± 0.509
3.908LysGlu: 3.908 ± 0.528
1.887LysPhe: 1.887 ± 0.379
4.38LysGly: 4.38 ± 0.719
1.078LysHis: 1.078 ± 0.359
2.224LysIle: 2.224 ± 0.355
2.493LysLys: 2.493 ± 0.471
5.458LysLeu: 5.458 ± 0.513
1.954LysMet: 1.954 ± 0.404
1.954LysAsn: 1.954 ± 0.309
3.302LysPro: 3.302 ± 0.452
2.561LysGln: 2.561 ± 0.686
3.437LysArg: 3.437 ± 0.509
2.83LysSer: 2.83 ± 0.403
2.763LysThr: 2.763 ± 0.361
3.302LysVal: 3.302 ± 0.417
0.876LysTrp: 0.876 ± 0.235
2.022LysTyr: 2.022 ± 0.425
0.0LysXaa: 0.0 ± 0.0
Leu
7.817LeuAla: 7.817 ± 1.012
1.146LeuCys: 1.146 ± 0.368
5.323LeuAsp: 5.323 ± 0.718
5.189LeuGlu: 5.189 ± 0.602
2.224LeuPhe: 2.224 ± 0.343
6.065LeuGly: 6.065 ± 0.571
2.022LeuHis: 2.022 ± 0.399
4.515LeuIle: 4.515 ± 0.51
3.571LeuLys: 3.571 ± 0.549
5.997LeuLeu: 5.997 ± 0.995
2.695LeuMet: 2.695 ± 0.315
3.639LeuAsn: 3.639 ± 0.506
4.043LeuPro: 4.043 ± 0.47
3.639LeuGln: 3.639 ± 0.481
4.515LeuArg: 4.515 ± 0.596
4.245LeuSer: 4.245 ± 0.59
5.189LeuThr: 5.189 ± 0.566
5.728LeuVal: 5.728 ± 0.637
0.876LeuTrp: 0.876 ± 0.34
2.022LeuTyr: 2.022 ± 0.351
0.0LeuXaa: 0.0 ± 0.0
Met
3.032MetAla: 3.032 ± 0.475
0.27MetCys: 0.27 ± 0.142
1.415MetAsp: 1.415 ± 0.292
2.156MetGlu: 2.156 ± 0.493
1.617MetPhe: 1.617 ± 0.264
2.291MetGly: 2.291 ± 0.423
0.741MetHis: 0.741 ± 0.266
0.876MetIle: 0.876 ± 0.257
2.089MetLys: 2.089 ± 0.518
2.628MetLeu: 2.628 ± 0.592
1.28MetMet: 1.28 ± 0.372
1.482MetAsn: 1.482 ± 0.343
1.348MetPro: 1.348 ± 0.26
1.348MetGln: 1.348 ± 0.241
1.415MetArg: 1.415 ± 0.364
2.291MetSer: 2.291 ± 0.357
2.224MetThr: 2.224 ± 0.256
2.089MetVal: 2.089 ± 0.352
0.472MetTrp: 0.472 ± 0.158
0.943MetTyr: 0.943 ± 0.24
0.0MetXaa: 0.0 ± 0.0
Asn
3.639AsnAla: 3.639 ± 0.744
0.27AsnCys: 0.27 ± 0.158
1.954AsnAsp: 1.954 ± 0.395
2.089AsnGlu: 2.089 ± 0.35
1.752AsnPhe: 1.752 ± 0.222
4.178AsnGly: 4.178 ± 0.609
0.876AsnHis: 0.876 ± 0.29
2.224AsnIle: 2.224 ± 0.417
2.561AsnLys: 2.561 ± 0.406
3.841AsnLeu: 3.841 ± 0.493
1.55AsnMet: 1.55 ± 0.291
2.156AsnAsn: 2.156 ± 0.611
3.1AsnPro: 3.1 ± 0.592
1.55AsnGln: 1.55 ± 0.321
2.898AsnArg: 2.898 ± 0.441
2.561AsnSer: 2.561 ± 0.393
2.83AsnThr: 2.83 ± 0.446
2.156AsnVal: 2.156 ± 0.278
0.809AsnTrp: 0.809 ± 0.209
1.146AsnTyr: 1.146 ± 0.315
0.0AsnXaa: 0.0 ± 0.0
Pro
3.976ProAla: 3.976 ± 0.528
0.472ProCys: 0.472 ± 0.203
2.628ProAsp: 2.628 ± 0.452
3.504ProGlu: 3.504 ± 0.602
1.617ProPhe: 1.617 ± 0.388
2.358ProGly: 2.358 ± 0.382
1.011ProHis: 1.011 ± 0.295
2.022ProIle: 2.022 ± 0.435
2.156ProLys: 2.156 ± 0.331
3.774ProLeu: 3.774 ± 0.611
1.348ProMet: 1.348 ± 0.364
2.628ProAsn: 2.628 ± 0.428
1.752ProPro: 1.752 ± 0.305
2.022ProGln: 2.022 ± 0.357
1.954ProArg: 1.954 ± 0.413
2.426ProSer: 2.426 ± 0.393
2.763ProThr: 2.763 ± 0.446
3.976ProVal: 3.976 ± 0.567
1.078ProTrp: 1.078 ± 0.284
1.482ProTyr: 1.482 ± 0.304
0.0ProXaa: 0.0 ± 0.0
Gln
5.189GlnAla: 5.189 ± 0.655
0.606GlnCys: 0.606 ± 0.209
1.819GlnAsp: 1.819 ± 0.448
2.358GlnGlu: 2.358 ± 0.415
2.156GlnPhe: 2.156 ± 0.468
3.235GlnGly: 3.235 ± 0.38
1.415GlnHis: 1.415 ± 0.334
2.156GlnIle: 2.156 ± 0.387
2.291GlnLys: 2.291 ± 0.413
3.235GlnLeu: 3.235 ± 0.502
2.291GlnMet: 2.291 ± 0.505
1.415GlnAsn: 1.415 ± 0.312
1.348GlnPro: 1.348 ± 0.377
2.83GlnGln: 2.83 ± 0.523
2.561GlnArg: 2.561 ± 0.383
2.763GlnSer: 2.763 ± 0.54
2.561GlnThr: 2.561 ± 0.463
3.032GlnVal: 3.032 ± 0.566
0.539GlnTrp: 0.539 ± 0.149
1.617GlnTyr: 1.617 ± 0.302
0.0GlnXaa: 0.0 ± 0.0
Arg
4.38ArgAla: 4.38 ± 0.843
0.337ArgCys: 0.337 ± 0.149
3.774ArgAsp: 3.774 ± 0.442
3.706ArgGlu: 3.706 ± 0.643
2.156ArgPhe: 2.156 ± 0.486
4.313ArgGly: 4.313 ± 0.545
1.146ArgHis: 1.146 ± 0.217
2.763ArgIle: 2.763 ± 0.286
2.83ArgLys: 2.83 ± 0.364
5.054ArgLeu: 5.054 ± 0.488
1.685ArgMet: 1.685 ± 0.428
3.167ArgAsn: 3.167 ± 0.432
1.482ArgPro: 1.482 ± 0.311
2.561ArgGln: 2.561 ± 0.45
2.493ArgArg: 2.493 ± 0.434
2.83ArgSer: 2.83 ± 0.326
3.774ArgThr: 3.774 ± 0.697
4.784ArgVal: 4.784 ± 0.605
0.741ArgTrp: 0.741 ± 0.227
1.685ArgTyr: 1.685 ± 0.316
0.0ArgXaa: 0.0 ± 0.0
Ser
4.919SerAla: 4.919 ± 0.652
0.606SerCys: 0.606 ± 0.204
3.369SerAsp: 3.369 ± 0.433
3.369SerGlu: 3.369 ± 0.39
2.561SerPhe: 2.561 ± 0.348
3.774SerGly: 3.774 ± 0.426
0.876SerHis: 0.876 ± 0.268
3.571SerIle: 3.571 ± 0.463
2.83SerLys: 2.83 ± 0.448
4.447SerLeu: 4.447 ± 0.508
1.954SerMet: 1.954 ± 0.352
1.954SerAsn: 1.954 ± 0.429
2.358SerPro: 2.358 ± 0.406
2.561SerGln: 2.561 ± 0.439
3.032SerArg: 3.032 ± 0.391
2.965SerSer: 2.965 ± 0.591
2.898SerThr: 2.898 ± 0.453
2.898SerVal: 2.898 ± 0.478
0.741SerTrp: 0.741 ± 0.208
2.291SerTyr: 2.291 ± 0.336
0.0SerXaa: 0.0 ± 0.0
Thr
5.054ThrAla: 5.054 ± 0.696
0.539ThrCys: 0.539 ± 0.195
3.167ThrAsp: 3.167 ± 0.432
3.369ThrGlu: 3.369 ± 0.435
3.167ThrPhe: 3.167 ± 0.477
5.66ThrGly: 5.66 ± 0.481
1.617ThrHis: 1.617 ± 0.325
3.437ThrIle: 3.437 ± 0.441
3.1ThrLys: 3.1 ± 0.471
4.717ThrLeu: 4.717 ± 0.506
2.022ThrMet: 2.022 ± 0.381
2.695ThrAsn: 2.695 ± 0.49
3.504ThrPro: 3.504 ± 0.462
2.898ThrGln: 2.898 ± 0.414
3.1ThrArg: 3.1 ± 0.332
3.1ThrSer: 3.1 ± 0.481
3.235ThrThr: 3.235 ± 0.548
3.302ThrVal: 3.302 ± 0.659
0.404ThrTrp: 0.404 ± 0.169
1.819ThrTyr: 1.819 ± 0.343
0.0ThrXaa: 0.0 ± 0.0
Val
5.66ValAla: 5.66 ± 0.731
0.606ValCys: 0.606 ± 0.188
4.043ValAsp: 4.043 ± 0.563
4.178ValGlu: 4.178 ± 0.654
2.089ValPhe: 2.089 ± 0.464
4.852ValGly: 4.852 ± 0.545
1.617ValHis: 1.617 ± 0.4
3.302ValIle: 3.302 ± 0.485
4.38ValLys: 4.38 ± 0.551
4.313ValLeu: 4.313 ± 0.666
2.156ValMet: 2.156 ± 0.387
2.561ValAsn: 2.561 ± 0.515
3.1ValPro: 3.1 ± 0.608
2.763ValGln: 2.763 ± 0.598
3.369ValArg: 3.369 ± 0.428
3.504ValSer: 3.504 ± 0.441
4.38ValThr: 4.38 ± 0.636
5.526ValVal: 5.526 ± 0.606
1.348ValTrp: 1.348 ± 0.32
2.224ValTyr: 2.224 ± 0.456
0.0ValXaa: 0.0 ± 0.0
Trp
1.348TrpAla: 1.348 ± 0.34
0.27TrpCys: 0.27 ± 0.125
0.606TrpAsp: 0.606 ± 0.163
1.011TrpGlu: 1.011 ± 0.235
0.472TrpPhe: 0.472 ± 0.165
1.55TrpGly: 1.55 ± 0.246
0.27TrpHis: 0.27 ± 0.096
0.876TrpIle: 0.876 ± 0.25
0.606TrpLys: 0.606 ± 0.177
1.752TrpLeu: 1.752 ± 0.295
0.404TrpMet: 0.404 ± 0.137
0.674TrpAsn: 0.674 ± 0.192
0.943TrpPro: 0.943 ± 0.27
0.809TrpGln: 0.809 ± 0.232
0.876TrpArg: 0.876 ± 0.209
1.011TrpSer: 1.011 ± 0.265
0.809TrpThr: 0.809 ± 0.219
0.337TrpVal: 0.337 ± 0.179
0.472TrpTrp: 0.472 ± 0.182
0.539TrpTyr: 0.539 ± 0.253
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.561TyrAla: 2.561 ± 0.351
0.404TyrCys: 0.404 ± 0.16
1.887TyrAsp: 1.887 ± 0.286
1.819TyrGlu: 1.819 ± 0.403
0.809TyrPhe: 0.809 ± 0.263
3.1TyrGly: 3.1 ± 0.427
0.539TyrHis: 0.539 ± 0.184
1.415TyrIle: 1.415 ± 0.351
1.819TyrLys: 1.819 ± 0.403
2.156TyrLeu: 2.156 ± 0.265
1.146TyrMet: 1.146 ± 0.253
2.224TyrAsn: 2.224 ± 0.346
1.482TyrPro: 1.482 ± 0.473
1.482TyrGln: 1.482 ± 0.294
2.426TyrArg: 2.426 ± 0.396
2.291TyrSer: 2.291 ± 0.426
2.089TyrThr: 2.089 ± 0.323
1.617TyrVal: 1.617 ± 0.535
0.472TyrTrp: 0.472 ± 0.191
0.809TyrTyr: 0.809 ± 0.228
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 46 proteins (14841 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski