Bacillus yapensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4414 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S0KI83|A0A3S0KI83_9BACI ABC transporter ATP-binding protein OS=Bacillus yapensis OX=2492960 GN=EKG37_11215 PE=4 SV=1
MM1 pKa = 7.89KK2 pKa = 10.13FRR4 pKa = 11.84KK5 pKa = 9.49HH6 pKa = 6.44KK7 pKa = 10.12GACCPPCPIVQEE19 pKa = 4.4TICGNFNGPLEE30 pKa = 4.39GSEE33 pKa = 4.19VWSATAGSYY42 pKa = 10.55RR43 pKa = 11.84SGTFQLFNDD52 pKa = 4.22PFSTGVVTAAGDD64 pKa = 4.73AIPPITLSAAPGNTDD79 pKa = 2.96SQSVNNPTSFTISASLGDD97 pKa = 3.65SGTYY101 pKa = 10.34CITLFRR107 pKa = 11.84NLCNVPPPLVCNVFAYY123 pKa = 10.09VVNSNDD129 pKa = 3.22NTVSVVDD136 pKa = 3.67TATNTVVATIPVGSIPDD153 pKa = 3.65EE154 pKa = 4.27VAFTPDD160 pKa = 2.7GSTAYY165 pKa = 8.74VTNFGSDD172 pKa = 3.04NVTVIDD178 pKa = 3.77TATNNVIATIPVGNGPIEE196 pKa = 4.19VAFTPDD202 pKa = 2.65GTRR205 pKa = 11.84AYY207 pKa = 10.52VSNEE211 pKa = 3.52QASTVSVIDD220 pKa = 3.46TATNTVIATIPVGTTPFGVAITPDD244 pKa = 3.23GTRR247 pKa = 11.84VYY249 pKa = 10.31VANQGASSISVINTATNTVIATIPDD274 pKa = 3.75GVNPQQIAITPDD286 pKa = 2.87GTRR289 pKa = 11.84AYY291 pKa = 9.54IPNGTANTVTVLDD304 pKa = 3.67IATNTVIATLPAGGDD319 pKa = 3.68PEE321 pKa = 4.79AVAITPNGTRR331 pKa = 11.84AYY333 pKa = 7.91VTNVLGDD340 pKa = 3.92SVTVIDD346 pKa = 4.04TATNTVIATVPVGDD360 pKa = 4.19SPRR363 pKa = 11.84GVDD366 pKa = 3.16ITPDD370 pKa = 2.95GTLAYY375 pKa = 8.59VTNQGSDD382 pKa = 3.36SVSVIDD388 pKa = 3.76VATNTVIDD396 pKa = 3.95TFAVGNGPRR405 pKa = 11.84GISFVQICTEE415 pKa = 4.1GG416 pKa = 3.11

Molecular weight: 42.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A431VSC8|A0A431VSC8_9BACI Nucleoid occlusion protein OS=Bacillus yapensis OX=2492960 GN=noc PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.59RR3 pKa = 11.84TFQPNKK9 pKa = 8.24RR10 pKa = 11.84KK11 pKa = 9.6RR12 pKa = 11.84SKK14 pKa = 9.59VHH16 pKa = 5.83GFRR19 pKa = 11.84SRR21 pKa = 11.84MSSANGRR28 pKa = 11.84KK29 pKa = 8.7VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.39GRR39 pKa = 11.84KK40 pKa = 8.52VLSAA44 pKa = 4.05

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4414

0

4414

1278922

14

1490

289.7

32.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.696 ± 0.039

0.708 ± 0.009

5.035 ± 0.029

7.918 ± 0.047

4.673 ± 0.034

6.822 ± 0.04

1.997 ± 0.016

8.012 ± 0.043

7.016 ± 0.036

9.849 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.667 ± 0.02

4.528 ± 0.027

3.534 ± 0.022

3.642 ± 0.027

3.985 ± 0.028

5.952 ± 0.027

5.369 ± 0.03

7.024 ± 0.032

1.035 ± 0.016

3.538 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski