Alces alces faeces associated circular virus MP65
Average proteome isoelectric point is 7.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z5CK43|A0A2Z5CK43_9VIRU Replication associated protein OS=Alces alces faeces associated circular virus MP65 OX=2219143 PE=4 SV=1
MM1 pKa = 6.73 VQQIADD7 pKa = 3.8 GLAAGAQEE15 pKa = 4.31 PADD18 pKa = 3.61 IPGVTGPGQRR28 pKa = 11.84 RR29 pKa = 11.84 KK30 pKa = 9.42 GTFRR34 pKa = 11.84 CKK36 pKa = 9.31 NQRR39 pKa = 11.84 LFLTYY44 pKa = 10.41 SQVGSGWDD52 pKa = 3.74 PQSLTDD58 pKa = 3.94 DD59 pKa = 4.32 LEE61 pKa = 4.83 RR62 pKa = 11.84 LGAVHH67 pKa = 7.35 RR68 pKa = 11.84 LGKK71 pKa = 9.75 EE72 pKa = 3.51 LHH74 pKa = 5.9 QDD76 pKa = 3.14 GGIHH80 pKa = 4.51 YY81 pKa = 9.68 HH82 pKa = 6.77 CYY84 pKa = 10.42 VDD86 pKa = 4.54 FGRR89 pKa = 11.84 PLEE92 pKa = 4.33 FEE94 pKa = 4.22 NCHH97 pKa = 5.68 KK98 pKa = 10.32 FCYY101 pKa = 9.92 GGPPSAGCPKK111 pKa = 10.39 GGHH114 pKa = 6.08 CNILVIRR121 pKa = 11.84 RR122 pKa = 11.84 TPHH125 pKa = 6.14 HH126 pKa = 6.24 AWDD129 pKa = 3.87 YY130 pKa = 10.83 AGKK133 pKa = 9.98 DD134 pKa = 3.08 GDD136 pKa = 4.57 VISEE140 pKa = 4.0 NCPRR144 pKa = 11.84 PLVVKK149 pKa = 9.97 GQSEE153 pKa = 4.58 RR154 pKa = 11.84 KK155 pKa = 9.96 AEE157 pKa = 3.77 WVYY160 pKa = 11.33 CLDD163 pKa = 3.35 ATGFDD168 pKa = 3.54 NFYY171 pKa = 11.31 EE172 pKa = 4.53 RR173 pKa = 11.84 IKK175 pKa = 11.15 DD176 pKa = 3.49 VDD178 pKa = 3.64 PASLVKK184 pKa = 10.63 SSFSVEE190 pKa = 3.72 HH191 pKa = 5.79 CAKK194 pKa = 10.4 RR195 pKa = 11.84 LFRR198 pKa = 11.84 QRR200 pKa = 11.84 QPEE203 pKa = 4.52 TFTPIDD209 pKa = 3.48 GLQFHH214 pKa = 6.85 WEE216 pKa = 4.22 SFPKK220 pKa = 9.06 VAQWVLRR227 pKa = 11.84 VLPDD231 pKa = 3.32 DD232 pKa = 3.92 RR233 pKa = 11.84 QLVGGRR239 pKa = 11.84 RR240 pKa = 11.84 PSLILWGASLHH251 pKa = 6.3 GKK253 pKa = 7.84 TDD255 pKa = 4.02 LARR258 pKa = 11.84 SLGNHH263 pKa = 4.53 IHH265 pKa = 6.72 FGNDD269 pKa = 2.79 FSLAEE274 pKa = 4.01 LLAVGVEE281 pKa = 4.14 NVDD284 pKa = 4.55 FGVLDD289 pKa = 4.34 DD290 pKa = 6.0 LDD292 pKa = 3.73 WTDD295 pKa = 5.92 PILKK299 pKa = 9.48 GNKK302 pKa = 7.52 YY303 pKa = 9.94 KK304 pKa = 10.86 AWLGCQEE311 pKa = 5.27 HH312 pKa = 6.83 FICTDD317 pKa = 2.54 KK318 pKa = 11.22 YY319 pKa = 11.0 LRR321 pKa = 11.84 KK322 pKa = 9.9 EE323 pKa = 4.04 SIKK326 pKa = 9.87 WGKK329 pKa = 8.99 PCIFLSNDD337 pKa = 3.53 DD338 pKa = 3.67 PTEE341 pKa = 4.12 SLKK344 pKa = 11.15 HH345 pKa = 6.8 SDD347 pKa = 4.04 FLWIQKK353 pKa = 9.15 NCWIVEE359 pKa = 4.26 VPDD362 pKa = 3.77 GQRR365 pKa = 11.84 IASRR369 pKa = 11.84 PSVFEE374 pKa = 3.85
Molecular weight: 42.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.153
IPC2_protein 6.122
IPC_protein 6.211
Toseland 6.453
ProMoST 6.517
Dawson 6.576
Bjellqvist 6.561
Wikipedia 6.605
Rodwell 6.576
Grimsley 6.561
Solomon 6.59
Lehninger 6.59
Nozaki 6.883
DTASelect 7.029
Thurlkill 7.059
EMBOSS 7.029
Sillero 6.985
Patrickios 4.177
IPC_peptide 6.605
IPC2_peptide 6.868
IPC2.peptide.svr19 6.808
Protein with the highest isoelectric point:
>tr|A0A2Z5CK43|A0A2Z5CK43_9VIRU Replication associated protein OS=Alces alces faeces associated circular virus MP65 OX=2219143 PE=4 SV=1
MM1 pKa = 6.75 NQARR5 pKa = 11.84 RR6 pKa = 11.84 DD7 pKa = 3.55 RR8 pKa = 11.84 EE9 pKa = 3.69 LALFQRR15 pKa = 11.84 GPDD18 pKa = 3.13 LWINRR23 pKa = 11.84 LASHH27 pKa = 6.03 TTRR30 pKa = 11.84 TDD32 pKa = 3.11 DD33 pKa = 3.78 PLGIKK38 pKa = 10.41 YY39 pKa = 9.96 EE40 pKa = 4.15 VGLPSTPRR48 pKa = 11.84 EE49 pKa = 3.52 ARR51 pKa = 11.84 LFVFQIFGQHH61 pKa = 5.74 RR62 pKa = 11.84 RR63 pKa = 11.84 TLPLVLKK70 pKa = 10.56 GLEE73 pKa = 4.02 RR74 pKa = 11.84 EE75 pKa = 4.55 LNGTAPFYY83 pKa = 10.79 PRR85 pKa = 11.84 MVSSQATALAQLWKK99 pKa = 9.87 NKK101 pKa = 9.28 KK102 pKa = 8.67 MPAKK106 pKa = 9.71 KK107 pKa = 9.9 SSRR110 pKa = 11.84 GGIGKK115 pKa = 9.13 AAKK118 pKa = 9.82 AKK120 pKa = 9.95 KK121 pKa = 10.34 NYY123 pKa = 9.48 GATPAFNRR131 pKa = 11.84 ALTAAVQKK139 pKa = 10.98 VEE141 pKa = 4.2 ARR143 pKa = 11.84 SQEE146 pKa = 4.02 TTYY149 pKa = 10.74 SQSGVYY155 pKa = 10.25 LNAAQSTFTQTGYY168 pKa = 8.74 TLSGLNQSAAYY179 pKa = 9.87 AVGTTNTRR187 pKa = 11.84 ANATNQVFAFPICPLAQVGNSSTPGYY213 pKa = 10.36 RR214 pKa = 11.84 KK215 pKa = 9.34 GQRR218 pKa = 11.84 INPVGFRR225 pKa = 11.84 FSIQHH230 pKa = 5.85 DD231 pKa = 3.63 QSLATMSATYY241 pKa = 9.86 KK242 pKa = 9.42 WALIRR247 pKa = 11.84 NQGGTLSGNTGPVITQTNAITMFVGLVQGPLASAGGPNGALPNGDD292 pKa = 3.99 FASSMRR298 pKa = 11.84 WNRR301 pKa = 11.84 QAWTVKK307 pKa = 10.2 KK308 pKa = 10.07 QGSYY312 pKa = 9.84 TMPAALARR320 pKa = 11.84 DD321 pKa = 4.13 NNTAPASATAPPFHH335 pKa = 6.95 NSSKK339 pKa = 9.63 TVTHH343 pKa = 7.11 FIPIRR348 pKa = 11.84 DD349 pKa = 3.95 SHH351 pKa = 6.55 WDD353 pKa = 3.61 YY354 pKa = 7.96 PTPTAIANIKK364 pKa = 9.76 GGDD367 pKa = 3.61 YY368 pKa = 11.13 YY369 pKa = 11.08 FVLWRR374 pKa = 11.84 EE375 pKa = 3.82 GSADD379 pKa = 3.49 QFIGNDD385 pKa = 4.43 FINFICEE392 pKa = 4.25 LSFKK396 pKa = 11.03 DD397 pKa = 3.55 PP398 pKa = 3.77
Molecular weight: 43.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.268
IPC2_protein 9.648
IPC_protein 10.014
Toseland 10.292
ProMoST 9.984
Dawson 10.467
Bjellqvist 10.145
Wikipedia 10.657
Rodwell 10.833
Grimsley 10.54
Solomon 10.511
Lehninger 10.467
Nozaki 10.277
DTASelect 10.145
Thurlkill 10.321
EMBOSS 10.687
Sillero 10.379
Patrickios 10.467
IPC_peptide 10.511
IPC2_peptide 8.887
IPC2.peptide.svr19 8.707
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
772
374
398
386.0
42.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.549 ± 2.24
1.943 ± 1.072
5.44 ± 1.618
3.627 ± 1.017
5.052 ± 0.02
8.808 ± 0.385
2.72 ± 0.903
4.404 ± 0.099
5.311 ± 0.4
8.031 ± 0.741
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.036 ± 0.538
4.663 ± 1.205
6.088 ± 0.144
5.181 ± 0.445
6.218 ± 0.047
6.347 ± 0.512
6.088 ± 2.014
5.311 ± 0.774
2.332 ± 0.426
2.85 ± 0.31
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here