Alistipes dispar
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2408 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Y1X0X4|A0A4Y1X0X4_9BACT Nudix hydrolase domain-containing protein OS=Alistipes dispar OX=2585119 GN=A5CPEGH6_05960 PE=4 SV=1
MM1 pKa = 8.02 ALMLALPLFAAGCIIDD17 pKa = 4.88 DD18 pKa = 4.58 PEE20 pKa = 4.96 PGPNLSGDD28 pKa = 3.06 WTEE31 pKa = 4.33 PEE33 pKa = 4.3 EE34 pKa = 4.21 PAGPEE39 pKa = 3.68 TFITVDD45 pKa = 3.59 GDD47 pKa = 3.61 FSDD50 pKa = 3.99 WDD52 pKa = 4.63 AIPADD57 pKa = 4.16 EE58 pKa = 5.21 LVTATVPDD66 pKa = 3.99 DD67 pKa = 3.62 AFYY70 pKa = 11.16 SAGRR74 pKa = 11.84 RR75 pKa = 11.84 LKK77 pKa = 10.15 VYY79 pKa = 10.9 AGATYY84 pKa = 10.37 INIYY88 pKa = 10.3 LEE90 pKa = 4.18 YY91 pKa = 10.67 SEE93 pKa = 6.13 AEE95 pKa = 3.89 QPVQIMHH102 pKa = 7.34 LYY104 pKa = 10.05 LDD106 pKa = 3.6 SDD108 pKa = 3.8 MAFDD112 pKa = 4.28 GEE114 pKa = 4.43 GRR116 pKa = 11.84 PTTGMGNAAWTNDD129 pKa = 3.1 GADD132 pKa = 3.2 ILFEE136 pKa = 4.31 GMLRR140 pKa = 11.84 DD141 pKa = 3.86 DD142 pKa = 4.04 TGAPTAYY149 pKa = 10.26 APTIFTWSGEE159 pKa = 4.05 PLSGGWDD166 pKa = 3.07 WTEE169 pKa = 3.54 AVAAGAGICSNCEE182 pKa = 3.68 PVEE185 pKa = 4.01 LPNGNMAIEE194 pKa = 4.09 MTIARR199 pKa = 11.84 SAVPGLGQSFRR210 pKa = 11.84 LGVGLQYY217 pKa = 11.21 DD218 pKa = 4.01 WNDD221 pKa = 2.95 IAYY224 pKa = 9.3 LPAGSAVEE232 pKa = 4.11 EE233 pKa = 4.2 NGEE236 pKa = 4.15 PKK238 pKa = 10.45 HH239 pKa = 6.05 GAVEE243 pKa = 3.96 NLLIGAKK250 pKa = 9.8 SGPAPEE256 pKa = 4.23 VLITADD262 pKa = 3.88 GDD264 pKa = 3.67 FSDD267 pKa = 4.15 WEE269 pKa = 4.26 KK270 pKa = 11.24 VPASEE275 pKa = 4.2 MTVATVPDD283 pKa = 3.73 DD284 pKa = 4.08 AYY286 pKa = 11.38 YY287 pKa = 10.94 KK288 pKa = 10.07 AGKK291 pKa = 8.12 VAKK294 pKa = 9.53 VYY296 pKa = 10.86 AGEE299 pKa = 4.41 TYY301 pKa = 10.06 VNFYY305 pKa = 11.11 VEE307 pKa = 4.63 YY308 pKa = 9.51 EE309 pKa = 4.28 DD310 pKa = 5.45 SAEE313 pKa = 3.95 QPVQIMHH320 pKa = 7.34 LYY322 pKa = 10.05 LDD324 pKa = 3.6 SDD326 pKa = 3.8 MAFDD330 pKa = 4.28 GEE332 pKa = 4.43 GRR334 pKa = 11.84 PTTGMGNAAWTNDD347 pKa = 3.1 GADD350 pKa = 3.16 ILFEE354 pKa = 4.16 GMVVDD359 pKa = 5.62 DD360 pKa = 5.21 AGAPAAYY367 pKa = 10.01 DD368 pKa = 3.32 PTIFNWTGEE377 pKa = 4.2 NLSGGWDD384 pKa = 3.31 WTEE387 pKa = 3.66 LLAAGSGVCSASKK400 pKa = 10.14 PVEE403 pKa = 3.93 LEE405 pKa = 3.72 NGRR408 pKa = 11.84 KK409 pKa = 8.86 AVEE412 pKa = 4.2 VVVVRR417 pKa = 11.84 SSIPNLGSRR426 pKa = 11.84 FRR428 pKa = 11.84 VGIGLQYY435 pKa = 11.01 DD436 pKa = 3.83 WNDD439 pKa = 2.76 IAYY442 pKa = 8.67 IPAGSAVEE450 pKa = 3.99 EE451 pKa = 4.31 NGVVGHH457 pKa = 6.15 GAVEE461 pKa = 3.94 NMLVGRR467 pKa = 4.74
Molecular weight: 49.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.705
IPC2_protein 3.821
IPC_protein 3.821
Toseland 3.617
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.706
Rodwell 3.643
Grimsley 3.516
Solomon 3.795
Lehninger 3.745
Nozaki 3.91
DTASelect 4.113
Thurlkill 3.656
EMBOSS 3.719
Sillero 3.935
Patrickios 1.367
IPC_peptide 3.795
IPC2_peptide 3.923
IPC2.peptide.svr19 3.837
Protein with the highest isoelectric point:
>tr|A0A4Y1X0M4|A0A4Y1X0M4_9BACT Uncharacterized protein OS=Alistipes dispar OX=2585119 GN=A5CPEGH6_04860 PE=4 SV=1
MM1 pKa = 7.32 TARR4 pKa = 11.84 CGGVGRR10 pKa = 11.84 TLRR13 pKa = 11.84 RR14 pKa = 11.84 AAARR18 pKa = 11.84 LLLVSLAALAAAACGRR34 pKa = 11.84 TEE36 pKa = 3.92 PEE38 pKa = 3.76 AVRR41 pKa = 11.84 VEE43 pKa = 3.81 AVEE46 pKa = 3.94 RR47 pKa = 11.84 FEE49 pKa = 4.78 RR50 pKa = 11.84 LGSAGADD57 pKa = 2.62 AVLRR61 pKa = 11.84 VHH63 pKa = 6.69 NGTGHH68 pKa = 5.0 TLKK71 pKa = 10.2 IRR73 pKa = 11.84 RR74 pKa = 11.84 VRR76 pKa = 11.84 LDD78 pKa = 2.83 VFYY81 pKa = 10.86 AGKK84 pKa = 10.33 RR85 pKa = 11.84 VGTLRR90 pKa = 11.84 LHH92 pKa = 5.93 EE93 pKa = 4.23 PVRR96 pKa = 11.84 IPRR99 pKa = 11.84 RR100 pKa = 11.84 ATTLVGTRR108 pKa = 11.84 WRR110 pKa = 11.84 LEE112 pKa = 3.93 TSDD115 pKa = 3.73 PMAFYY120 pKa = 10.72 VVEE123 pKa = 4.08 RR124 pKa = 11.84 RR125 pKa = 11.84 LGEE128 pKa = 4.2 GDD130 pKa = 3.7 LSRR133 pKa = 11.84 IGLSYY138 pKa = 10.41 EE139 pKa = 3.48 LRR141 pKa = 11.84 ARR143 pKa = 11.84 SGVVPVKK150 pKa = 10.44 ISGDD154 pKa = 3.56 MMPLSEE160 pKa = 4.67 FLNTFGLSEE169 pKa = 4.77 DD170 pKa = 4.97 DD171 pKa = 3.03 IKK173 pKa = 11.4 EE174 pKa = 4.01 YY175 pKa = 11.2 LKK177 pKa = 11.14
Molecular weight: 19.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.346
IPC2_protein 9.487
IPC_protein 10.482
Toseland 10.511
ProMoST 10.394
Dawson 10.628
Bjellqvist 10.409
Wikipedia 10.891
Rodwell 10.657
Grimsley 10.701
Solomon 10.789
Lehninger 10.745
Nozaki 10.511
DTASelect 10.394
Thurlkill 10.526
EMBOSS 10.935
Sillero 10.57
Patrickios 10.365
IPC_peptide 10.789
IPC2_peptide 9.545
IPC2.peptide.svr19 8.653
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2408
0
2408
882180
41
2498
366.4
40.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.709 ± 0.065
1.274 ± 0.018
5.782 ± 0.033
6.795 ± 0.047
4.231 ± 0.03
8.017 ± 0.039
1.71 ± 0.02
5.33 ± 0.045
4.119 ± 0.05
9.063 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.452 ± 0.023
3.566 ± 0.041
4.343 ± 0.033
2.735 ± 0.026
7.316 ± 0.064
5.584 ± 0.041
5.597 ± 0.041
7.066 ± 0.042
1.328 ± 0.023
3.982 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here