Gluconobacter oxydans (strain 621H) (Gluconobacter suboxydans)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae; Gluconobacter; Gluconobacter oxydans

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2626 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5FS93|Q5FS93_GLUOX 6 7-dimethyl-8-ribityllumazine synthase OS=Gluconobacter oxydans (strain 621H) OX=290633 GN=ribH PE=3 SV=1
MM1 pKa = 7.47SSIINAVHH9 pKa = 5.79TAVSGLNAQSHH20 pKa = 6.39AFTDD24 pKa = 4.34LSNNIANSQTTGYY37 pKa = 9.63KK38 pKa = 10.4ASTTSFEE45 pKa = 5.37DD46 pKa = 3.9YY47 pKa = 9.44VTNNEE52 pKa = 4.17LANDD56 pKa = 4.45GEE58 pKa = 4.54SLSDD62 pKa = 3.48SVAATTRR69 pKa = 11.84QHH71 pKa = 6.48TDD73 pKa = 2.99NQCMVTTSTNSTALAISGNGFFNVVQATGQSNDD106 pKa = 3.29STPVLGTQQYY116 pKa = 6.44YY117 pKa = 8.86TRR119 pKa = 11.84NGDD122 pKa = 3.59FSQDD126 pKa = 3.07KK127 pKa = 10.73NGYY130 pKa = 8.72LVNTSGYY137 pKa = 8.81YY138 pKa = 10.12LEE140 pKa = 5.47GYY142 pKa = 9.83NVDD145 pKa = 3.64ATSGTLGTSLSAIQVPSTIAFRR167 pKa = 11.84PTEE170 pKa = 4.27STKK173 pKa = 10.15LTATGTLDD181 pKa = 3.27SSSSKK186 pKa = 9.35TVDD189 pKa = 3.1MSTTAYY195 pKa = 10.42DD196 pKa = 3.56STGAASTVDD205 pKa = 3.78LSWTKK210 pKa = 10.97LSDD213 pKa = 3.44TTWAVKK219 pKa = 10.1DD220 pKa = 3.96VNDD223 pKa = 3.8TTGTSNSATVTFDD236 pKa = 2.8SSGAVSSVTSQDD248 pKa = 3.06STGASSTSSSSFNYY262 pKa = 9.31TGSPQDD268 pKa = 3.39MTVSLSGVTLGSANTVSMTSDD289 pKa = 2.89SVTTGTFSGISMEE302 pKa = 4.27TDD304 pKa = 3.13GSVMATFDD312 pKa = 3.2NGYY315 pKa = 8.5SQLVAKK321 pKa = 10.53IPLTTFADD329 pKa = 4.12PNSLSAQNGQAYY341 pKa = 6.97TATSQSGSPQINAVNTNGAGTLTTGSIEE369 pKa = 4.31EE370 pKa = 4.47STTDD374 pKa = 3.46LTGDD378 pKa = 3.96LSKK381 pKa = 11.22LIVAQQAYY389 pKa = 8.94GANTKK394 pKa = 9.73VVSTADD400 pKa = 3.32QLLQTTLAMIQQ411 pKa = 3.18

Molecular weight: 42.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5FTT2|Q5FTT2_GLUOX Phosphogluconate dehydratase OS=Gluconobacter oxydans (strain 621H) OX=290633 GN=edd PE=3 SV=1
MM1 pKa = 7.54GFFRR5 pKa = 11.84TFRR8 pKa = 11.84DD9 pKa = 4.17GYY11 pKa = 10.55LVLRR15 pKa = 11.84FRR17 pKa = 11.84NWEE20 pKa = 3.78IQKK23 pKa = 9.53VVHH26 pKa = 5.44FHH28 pKa = 6.56GYY30 pKa = 9.86SPVSRR35 pKa = 11.84LRR37 pKa = 11.84SLDD40 pKa = 3.54LPRR43 pKa = 11.84SLVVRR48 pKa = 11.84RR49 pKa = 11.84VVALDD54 pKa = 3.21NRR56 pKa = 11.84VEE58 pKa = 4.11IEE60 pKa = 3.68VGLRR64 pKa = 11.84KK65 pKa = 9.87RR66 pKa = 11.84SAVCPDD72 pKa = 4.08CQCPTQRR79 pKa = 11.84IHH81 pKa = 7.38SFYY84 pKa = 10.36QRR86 pKa = 11.84KK87 pKa = 8.54LADD90 pKa = 4.65LPWQSRR96 pKa = 11.84PATLIVSVPRR106 pKa = 11.84FFCQTMLCPRR116 pKa = 11.84RR117 pKa = 11.84TFVMPLEE124 pKa = 5.08GITVRR129 pKa = 11.84HH130 pKa = 5.66GRR132 pKa = 11.84QTTRR136 pKa = 11.84LADD139 pKa = 3.18LHH141 pKa = 6.76YY142 pKa = 10.7YY143 pKa = 8.98IAHH146 pKa = 6.38TLGGSAGARR155 pKa = 11.84MTVRR159 pKa = 11.84LCCPISADD167 pKa = 3.52TLVRR171 pKa = 11.84RR172 pKa = 11.84LLSRR176 pKa = 11.84VQNTTKK182 pKa = 9.73GTALTRR188 pKa = 11.84VVGVDD193 pKa = 2.97DD194 pKa = 3.8WAWRR198 pKa = 11.84RR199 pKa = 11.84GHH201 pKa = 6.76HH202 pKa = 6.33YY203 pKa = 9.33GTIVVDD209 pKa = 3.89LEE211 pKa = 4.35KK212 pKa = 11.17NDD214 pKa = 4.57VIDD217 pKa = 5.12LLPDD221 pKa = 3.71RR222 pKa = 11.84DD223 pKa = 4.22ADD225 pKa = 3.92TLARR229 pKa = 11.84WLQVHH234 pKa = 6.72PGIEE238 pKa = 3.92IIARR242 pKa = 11.84DD243 pKa = 3.57RR244 pKa = 11.84AGTYY248 pKa = 10.7ADD250 pKa = 3.52GCRR253 pKa = 11.84RR254 pKa = 11.84GAPSALQVTDD264 pKa = 3.09RR265 pKa = 11.84WHH267 pKa = 6.23LLKK270 pKa = 10.8NLSDD274 pKa = 3.92AFVSILDD281 pKa = 3.7RR282 pKa = 11.84FTTTVRR288 pKa = 11.84TLTRR292 pKa = 11.84QMNVSTVVSKK302 pKa = 10.78ISEE305 pKa = 4.51TIRR308 pKa = 11.84AHH310 pKa = 6.13PEE312 pKa = 3.15EE313 pKa = 4.41AQAVVRR319 pKa = 11.84SASGEE324 pKa = 3.83RR325 pKa = 11.84RR326 pKa = 11.84DD327 pKa = 3.89ILFKK331 pKa = 10.74EE332 pKa = 4.05ALALKK337 pKa = 10.2AAGHH341 pKa = 6.64SIQAIATTIGVEE353 pKa = 3.96RR354 pKa = 11.84KK355 pKa = 7.01TVRR358 pKa = 11.84RR359 pKa = 11.84WFQRR363 pKa = 11.84GHH365 pKa = 5.9APPWKK370 pKa = 8.31RR371 pKa = 11.84TVPSRR376 pKa = 11.84SILVPYY382 pKa = 8.12TAHH385 pKa = 7.4LEE387 pKa = 4.29KK388 pKa = 10.3RR389 pKa = 11.84WSEE392 pKa = 3.84GCRR395 pKa = 11.84NSRR398 pKa = 11.84QLWRR402 pKa = 11.84EE403 pKa = 3.65LLEE406 pKa = 4.0QGFAGQYY413 pKa = 6.71GTVWKK418 pKa = 9.66WVATRR423 pKa = 11.84QRR425 pKa = 11.84CSAKK429 pKa = 9.6PVPSAHH435 pKa = 5.24VVAPRR440 pKa = 11.84GRR442 pKa = 11.84RR443 pKa = 11.84LARR446 pKa = 11.84LLLAEE451 pKa = 5.31DD452 pKa = 4.45PLSAGQEE459 pKa = 4.27GLLVPALLEE468 pKa = 4.45AEE470 pKa = 4.1PHH472 pKa = 6.28LAVTLCWLRR481 pKa = 11.84KK482 pKa = 8.18MQTLLCKK489 pKa = 10.5RR490 pKa = 11.84GDD492 pKa = 3.52TCTGGDD498 pKa = 3.65LKK500 pKa = 11.36ALLEE504 pKa = 4.29EE505 pKa = 5.0GKK507 pKa = 7.51TTLLSRR513 pKa = 11.84LIPALEE519 pKa = 4.11RR520 pKa = 11.84DD521 pKa = 3.44AAAIEE526 pKa = 4.3ASLVTPWTTSPVEE539 pKa = 4.1GQISRR544 pKa = 11.84LKK546 pKa = 9.4MIKK549 pKa = 8.82RR550 pKa = 11.84TMFGRR555 pKa = 11.84AGFEE559 pKa = 3.95LLRR562 pKa = 11.84ARR564 pKa = 11.84VLQPAA569 pKa = 3.85

Molecular weight: 64.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2626

0

2626

859884

26

1750

327.5

35.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.264 ± 0.062

1.029 ± 0.017

5.596 ± 0.039

5.356 ± 0.049

3.483 ± 0.029

8.349 ± 0.05

2.418 ± 0.023

4.93 ± 0.036

3.006 ± 0.037

10.317 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.538 ± 0.022

2.729 ± 0.034

5.602 ± 0.037

3.563 ± 0.027

7.213 ± 0.048

6.1 ± 0.043

5.897 ± 0.047

7.045 ± 0.042

1.381 ± 0.018

2.183 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski