Aphanomyces invadans

Taxonomy: cellular organisms; Eukaryota; Sar; Stramenopiles; Oomycota; Saprolegniales; Saprolegniaceae; Aphanomyces

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 19593 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A024TGX6|A0A024TGX6_9STRA Uncharacterized protein OS=Aphanomyces invadans OX=157072 GN=H310_12596 PE=4 SV=1
MM1 pKa = 7.55LNGEE5 pKa = 4.46SIDD8 pKa = 4.04YY9 pKa = 10.87VSGEE13 pKa = 3.9AAGDD17 pKa = 3.58GDD19 pKa = 4.66ADD21 pKa = 4.98AISGDD26 pKa = 4.39DD27 pKa = 3.62RR28 pKa = 11.84GEE30 pKa = 4.17MYY32 pKa = 10.26PSSSSSSSSAKK43 pKa = 10.25LYY45 pKa = 10.09SCRR48 pKa = 11.84FPVTSWDD55 pKa = 3.72GTGVASDD62 pKa = 3.97GASGVSLVPSCRR74 pKa = 11.84LFDD77 pKa = 3.75VSCEE81 pKa = 4.06GGPPAASDD89 pKa = 3.47WWCPSSPPFFSSLRR103 pKa = 11.84AGGRR107 pKa = 11.84TEE109 pKa = 5.65LLDD112 pKa = 4.5DD113 pKa = 3.91SS114 pKa = 4.62

Molecular weight: 11.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A024UDH9|A0A024UDH9_9STRA Uncharacterized protein OS=Aphanomyces invadans OX=157072 GN=H310_04715 PE=4 SV=1
MM1 pKa = 7.33LVLASVMLAVARR13 pKa = 11.84VLLVVVRR20 pKa = 11.84VLLAVARR27 pKa = 11.84VLLVVVRR34 pKa = 11.84VLLVVVRR41 pKa = 11.84VLLVVARR48 pKa = 11.84VLLAVARR55 pKa = 11.84VLLMVVRR62 pKa = 11.84VLVVVVRR69 pKa = 11.84VLLVVARR76 pKa = 11.84VLLVVVRR83 pKa = 11.84VLLVVVRR90 pKa = 11.84VLLVVARR97 pKa = 11.84VLLVVVLALALARR110 pKa = 11.84VMLWSSHH117 pKa = 4.64VPP119 pKa = 3.27

Molecular weight: 12.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15212

4381

19593

9628465

30

12680

491.4

54.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.537 ± 0.017

1.83 ± 0.01

5.821 ± 0.014

4.998 ± 0.016

3.698 ± 0.011

5.582 ± 0.021

3.254 ± 0.01

4.084 ± 0.011

4.554 ± 0.017

9.676 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.594 ± 0.007

3.289 ± 0.008

5.387 ± 0.018

4.11 ± 0.014

5.909 ± 0.016

7.706 ± 0.017

6.361 ± 0.017

7.784 ± 0.016

1.338 ± 0.007

2.489 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski