Acinetobacter phage vB_AbaP_PD-6A3
Average proteome isoelectric point is 6.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0S1RTN4|A0A0S1RTN4_9CAUD HNHc domain-containing protein OS=Acinetobacter phage vB_AbaP_PD-6A3 OX=1701807 GN=vBAbaPPD6A3_33 PE=4 SV=1
MM1 pKa = 7.59 AGKK4 pKa = 8.42 KK5 pKa = 8.41 TGASVSRR12 pKa = 11.84 LCLLHH17 pKa = 6.83 EE18 pKa = 4.88 LLVDD22 pKa = 3.61 MFIKK26 pKa = 10.58 DD27 pKa = 3.27 IQDD30 pKa = 4.16 AIEE33 pKa = 4.38 GDD35 pKa = 4.09 YY36 pKa = 11.13 PLASADD42 pKa = 3.47 KK43 pKa = 9.72 NVIVTFLKK51 pKa = 10.85 NEE53 pKa = 4.72 SITATPDD60 pKa = 3.14 ADD62 pKa = 3.57 GMEE65 pKa = 4.21 KK66 pKa = 10.43 LKK68 pKa = 11.23 EE69 pKa = 3.97 EE70 pKa = 4.39 LKK72 pKa = 11.0 DD73 pKa = 3.51 LSEE76 pKa = 4.13 AQRR79 pKa = 11.84 AKK81 pKa = 10.74 VDD83 pKa = 3.52 ALVTQVEE90 pKa = 4.57 SGQFDD95 pKa = 4.54 DD96 pKa = 5.91 LLGPIQQ102 pKa = 3.91
Molecular weight: 11.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.601
IPC2_protein 4.444
IPC_protein 4.355
Toseland 4.164
ProMoST 4.482
Dawson 4.317
Bjellqvist 4.469
Wikipedia 4.228
Rodwell 4.177
Grimsley 4.075
Solomon 4.317
Lehninger 4.266
Nozaki 4.431
DTASelect 4.635
Thurlkill 4.19
EMBOSS 4.24
Sillero 4.469
Patrickios 4.202
IPC_peptide 4.317
IPC2_peptide 4.444
IPC2.peptide.svr19 4.423
Protein with the highest isoelectric point:
>tr|A0A0S1S026|A0A0S1S026_9CAUD Uncharacterized protein OS=Acinetobacter phage vB_AbaP_PD-6A3 OX=1701807 GN=vBAbaPPD6A3_46 PE=4 SV=1
MM1 pKa = 7.85 RR2 pKa = 11.84 GYY4 pKa = 11.14 NYY6 pKa = 9.84 IAGDD10 pKa = 3.32 RR11 pKa = 11.84 VYY13 pKa = 10.08 RR14 pKa = 11.84 TVPNGLIPKK23 pKa = 7.21 GTKK26 pKa = 8.67 GTVGSCRR33 pKa = 11.84 MDD35 pKa = 3.19 DD36 pKa = 3.57 TVQVQFDD43 pKa = 3.7 NGRR46 pKa = 11.84 TWYY49 pKa = 10.14 CHH51 pKa = 5.67 SKK53 pKa = 10.38 FIKK56 pKa = 10.0 PLVRR60 pKa = 11.84 VVGG63 pKa = 3.72
Molecular weight: 7.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.211
IPC2_protein 9.37
IPC_protein 9.56
Toseland 9.765
ProMoST 9.619
Dawson 10.058
Bjellqvist 9.823
Wikipedia 10.277
Rodwell 10.277
Grimsley 10.16
Solomon 10.116
Lehninger 10.087
Nozaki 9.882
DTASelect 9.794
Thurlkill 9.882
EMBOSS 10.204
Sillero 9.999
Patrickios 7.527
IPC_peptide 10.116
IPC2_peptide 8.858
IPC2.peptide.svr19 8.193
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
48
0
48
12815
43
1032
267.0
29.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.194 ± 0.437
1.061 ± 0.183
6.391 ± 0.23
5.853 ± 0.267
3.387 ± 0.136
7.327 ± 0.286
2.076 ± 0.178
5.611 ± 0.234
6.883 ± 0.305
8.381 ± 0.286
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.677 ± 0.164
4.901 ± 0.268
3.597 ± 0.152
4.705 ± 0.27
4.791 ± 0.286
5.611 ± 0.264
6.25 ± 0.354
6.773 ± 0.289
1.264 ± 0.094
4.268 ± 0.243
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here