Amino acid dipepetide frequency for Escherichia phage Ayreon

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.401AlaAla: 12.401 ± 1.821
0.954AlaCys: 0.954 ± 0.252
5.87AlaAsp: 5.87 ± 0.688
5.136AlaGlu: 5.136 ± 0.73
3.449AlaPhe: 3.449 ± 0.518
8.072AlaGly: 8.072 ± 0.962
1.027AlaHis: 1.027 ± 0.285
5.283AlaIle: 5.283 ± 0.697
4.403AlaLys: 4.403 ± 0.903
7.925AlaLeu: 7.925 ± 0.925
3.522AlaMet: 3.522 ± 0.578
2.642AlaAsn: 2.642 ± 0.525
3.155AlaPro: 3.155 ± 0.495
3.522AlaGln: 3.522 ± 0.692
5.724AlaArg: 5.724 ± 0.805
7.191AlaSer: 7.191 ± 0.889
5.283AlaThr: 5.283 ± 0.746
6.824AlaVal: 6.824 ± 0.845
1.908AlaTrp: 1.908 ± 0.437
2.568AlaTyr: 2.568 ± 0.451
0.0AlaXaa: 0.0 ± 0.0
Cys
1.101CysAla: 1.101 ± 0.295
0.367CysCys: 0.367 ± 0.155
0.514CysAsp: 0.514 ± 0.181
0.587CysGlu: 0.587 ± 0.233
0.367CysPhe: 0.367 ± 0.164
1.174CysGly: 1.174 ± 0.299
0.587CysHis: 0.587 ± 0.249
0.22CysIle: 0.22 ± 0.122
0.734CysLys: 0.734 ± 0.239
1.541CysLeu: 1.541 ± 0.406
0.147CysMet: 0.147 ± 0.092
0.367CysAsn: 0.367 ± 0.144
0.367CysPro: 0.367 ± 0.215
0.734CysGln: 0.734 ± 0.302
1.101CysArg: 1.101 ± 0.317
0.734CysSer: 0.734 ± 0.25
0.807CysThr: 0.807 ± 0.235
0.66CysVal: 0.66 ± 0.22
0.367CysTrp: 0.367 ± 0.199
0.367CysTyr: 0.367 ± 0.17
0.0CysXaa: 0.0 ± 0.0
Asp
5.944AspAla: 5.944 ± 0.733
0.587AspCys: 0.587 ± 0.214
3.816AspAsp: 3.816 ± 0.484
2.568AspGlu: 2.568 ± 0.452
2.275AspPhe: 2.275 ± 0.376
5.87AspGly: 5.87 ± 0.663
0.514AspHis: 0.514 ± 0.156
3.449AspIle: 3.449 ± 0.514
2.715AspLys: 2.715 ± 0.462
5.503AspLeu: 5.503 ± 0.75
1.468AspMet: 1.468 ± 0.322
3.155AspAsn: 3.155 ± 0.399
2.275AspPro: 2.275 ± 0.497
2.055AspGln: 2.055 ± 0.45
2.348AspArg: 2.348 ± 0.417
3.155AspSer: 3.155 ± 0.47
2.055AspThr: 2.055 ± 0.347
3.742AspVal: 3.742 ± 0.554
1.614AspTrp: 1.614 ± 0.398
1.688AspTyr: 1.688 ± 0.284
0.0AspXaa: 0.0 ± 0.0
Glu
5.063GluAla: 5.063 ± 0.661
0.881GluCys: 0.881 ± 0.245
2.642GluAsp: 2.642 ± 0.43
3.302GluGlu: 3.302 ± 0.583
1.394GluPhe: 1.394 ± 0.33
2.935GluGly: 2.935 ± 0.482
1.101GluHis: 1.101 ± 0.289
3.522GluIle: 3.522 ± 0.575
2.935GluLys: 2.935 ± 0.464
6.164GluLeu: 6.164 ± 0.632
1.614GluMet: 1.614 ± 0.395
2.715GluAsn: 2.715 ± 0.468
2.715GluPro: 2.715 ± 0.576
3.596GluGln: 3.596 ± 0.788
4.476GluArg: 4.476 ± 0.819
3.302GluSer: 3.302 ± 0.644
4.256GluThr: 4.256 ± 0.511
3.375GluVal: 3.375 ± 0.536
0.807GluTrp: 0.807 ± 0.255
1.321GluTyr: 1.321 ± 0.32
0.0GluXaa: 0.0 ± 0.0
Phe
2.495PheAla: 2.495 ± 0.459
0.734PheCys: 0.734 ± 0.277
1.908PheAsp: 1.908 ± 0.307
1.981PheGlu: 1.981 ± 0.395
0.587PhePhe: 0.587 ± 0.203
1.688PheGly: 1.688 ± 0.356
0.514PheHis: 0.514 ± 0.178
2.348PheIle: 2.348 ± 0.67
1.688PheLys: 1.688 ± 0.388
2.201PheLeu: 2.201 ± 0.63
1.394PheMet: 1.394 ± 0.319
1.688PheAsn: 1.688 ± 0.316
1.614PhePro: 1.614 ± 0.373
0.881PheGln: 0.881 ± 0.208
2.201PheArg: 2.201 ± 0.502
2.788PheSer: 2.788 ± 0.489
1.321PheThr: 1.321 ± 0.235
2.862PheVal: 2.862 ± 0.407
0.881PheTrp: 0.881 ± 0.199
0.881PheTyr: 0.881 ± 0.27
0.0PheXaa: 0.0 ± 0.0
Gly
6.237GlyAla: 6.237 ± 0.633
1.101GlyCys: 1.101 ± 0.266
4.99GlyAsp: 4.99 ± 0.84
4.256GlyGlu: 4.256 ± 0.569
2.568GlyPhe: 2.568 ± 0.506
5.063GlyGly: 5.063 ± 0.677
1.247GlyHis: 1.247 ± 0.327
3.669GlyIle: 3.669 ± 0.5
5.43GlyLys: 5.43 ± 0.647
4.549GlyLeu: 4.549 ± 0.61
2.495GlyMet: 2.495 ± 0.38
4.183GlyAsn: 4.183 ± 0.725
1.541GlyPro: 1.541 ± 0.256
2.788GlyGln: 2.788 ± 0.486
3.962GlyArg: 3.962 ± 0.5
5.357GlySer: 5.357 ± 0.588
3.889GlyThr: 3.889 ± 0.827
6.237GlyVal: 6.237 ± 0.692
1.834GlyTrp: 1.834 ± 0.367
2.568GlyTyr: 2.568 ± 0.395
0.0GlyXaa: 0.0 ± 0.0
His
1.247HisAla: 1.247 ± 0.337
0.514HisCys: 0.514 ± 0.181
1.247HisAsp: 1.247 ± 0.394
1.101HisGlu: 1.101 ± 0.316
1.174HisPhe: 1.174 ± 0.27
1.614HisGly: 1.614 ± 0.371
0.514HisHis: 0.514 ± 0.221
1.174HisIle: 1.174 ± 0.337
0.807HisLys: 0.807 ± 0.213
1.541HisLeu: 1.541 ± 0.361
0.294HisMet: 0.294 ± 0.139
0.294HisAsn: 0.294 ± 0.207
0.587HisPro: 0.587 ± 0.216
0.807HisGln: 0.807 ± 0.227
1.027HisArg: 1.027 ± 0.3
1.247HisSer: 1.247 ± 0.337
0.66HisThr: 0.66 ± 0.226
1.027HisVal: 1.027 ± 0.282
0.734HisTrp: 0.734 ± 0.298
0.44HisTyr: 0.44 ± 0.174
0.0HisXaa: 0.0 ± 0.0
Ile
4.843IleAla: 4.843 ± 0.691
0.734IleCys: 0.734 ± 0.268
3.596IleAsp: 3.596 ± 0.437
2.862IleGlu: 2.862 ± 0.486
1.614IlePhe: 1.614 ± 0.438
3.522IleGly: 3.522 ± 0.66
0.66IleHis: 0.66 ± 0.255
2.788IleIle: 2.788 ± 0.557
2.495IleLys: 2.495 ± 0.438
2.935IleLeu: 2.935 ± 0.556
1.174IleMet: 1.174 ± 0.354
3.816IleAsn: 3.816 ± 0.755
2.715IlePro: 2.715 ± 0.634
1.908IleGln: 1.908 ± 0.387
4.549IleArg: 4.549 ± 0.634
3.742IleSer: 3.742 ± 0.621
4.183IleThr: 4.183 ± 0.705
2.055IleVal: 2.055 ± 0.38
0.294IleTrp: 0.294 ± 0.131
1.614IleTyr: 1.614 ± 0.334
0.0IleXaa: 0.0 ± 0.0
Lys
5.65LysAla: 5.65 ± 0.707
0.881LysCys: 0.881 ± 0.295
2.495LysAsp: 2.495 ± 0.438
3.155LysGlu: 3.155 ± 0.57
1.761LysPhe: 1.761 ± 0.416
4.549LysGly: 4.549 ± 0.633
0.734LysHis: 0.734 ± 0.22
2.862LysIle: 2.862 ± 0.542
3.009LysLys: 3.009 ± 0.583
4.476LysLeu: 4.476 ± 0.524
1.101LysMet: 1.101 ± 0.262
2.495LysAsn: 2.495 ± 0.374
1.834LysPro: 1.834 ± 0.48
2.348LysGln: 2.348 ± 0.325
2.935LysArg: 2.935 ± 0.46
3.375LysSer: 3.375 ± 0.441
3.155LysThr: 3.155 ± 0.467
4.036LysVal: 4.036 ± 0.5
0.734LysTrp: 0.734 ± 0.284
1.688LysTyr: 1.688 ± 0.29
0.0LysXaa: 0.0 ± 0.0
Leu
7.191LeuAla: 7.191 ± 0.761
1.174LeuCys: 1.174 ± 0.319
4.036LeuAsp: 4.036 ± 0.436
4.109LeuGlu: 4.109 ± 0.578
2.788LeuPhe: 2.788 ± 0.775
5.136LeuGly: 5.136 ± 0.594
2.348LeuHis: 2.348 ± 0.503
4.843LeuIle: 4.843 ± 0.807
4.696LeuLys: 4.696 ± 0.676
5.87LeuLeu: 5.87 ± 0.753
1.614LeuMet: 1.614 ± 0.305
4.843LeuAsn: 4.843 ± 0.482
4.109LeuPro: 4.109 ± 0.536
3.082LeuGln: 3.082 ± 0.473
6.311LeuArg: 6.311 ± 0.678
7.705LeuSer: 7.705 ± 1.072
6.237LeuThr: 6.237 ± 0.624
5.503LeuVal: 5.503 ± 0.623
1.101LeuTrp: 1.101 ± 0.305
1.981LeuTyr: 1.981 ± 0.4
0.0LeuXaa: 0.0 ± 0.0
Met
2.862MetAla: 2.862 ± 0.536
0.22MetCys: 0.22 ± 0.136
1.174MetAsp: 1.174 ± 0.278
1.394MetGlu: 1.394 ± 0.351
0.587MetPhe: 0.587 ± 0.195
1.394MetGly: 1.394 ± 0.369
0.44MetHis: 0.44 ± 0.191
0.734MetIle: 0.734 ± 0.33
1.101MetLys: 1.101 ± 0.345
3.009MetLeu: 3.009 ± 0.462
0.807MetMet: 0.807 ± 0.273
1.761MetAsn: 1.761 ± 0.347
1.541MetPro: 1.541 ± 0.329
1.321MetGln: 1.321 ± 0.293
1.614MetArg: 1.614 ± 0.315
1.541MetSer: 1.541 ± 0.325
2.348MetThr: 2.348 ± 0.444
1.761MetVal: 1.761 ± 0.342
0.22MetTrp: 0.22 ± 0.119
0.514MetTyr: 0.514 ± 0.251
0.0MetXaa: 0.0 ± 0.0
Asn
3.889AsnAla: 3.889 ± 0.585
0.587AsnCys: 0.587 ± 0.241
2.421AsnAsp: 2.421 ± 0.419
2.788AsnGlu: 2.788 ± 0.418
1.321AsnPhe: 1.321 ± 0.274
4.476AsnGly: 4.476 ± 0.609
1.027AsnHis: 1.027 ± 0.34
2.642AsnIle: 2.642 ± 0.387
2.275AsnLys: 2.275 ± 0.392
2.935AsnLeu: 2.935 ± 0.48
1.027AsnMet: 1.027 ± 0.232
1.981AsnAsn: 1.981 ± 0.357
2.348AsnPro: 2.348 ± 0.506
1.614AsnGln: 1.614 ± 0.273
2.568AsnArg: 2.568 ± 0.512
3.229AsnSer: 3.229 ± 0.641
2.275AsnThr: 2.275 ± 0.424
2.568AsnVal: 2.568 ± 0.408
0.44AsnTrp: 0.44 ± 0.161
1.247AsnTyr: 1.247 ± 0.316
0.0AsnXaa: 0.0 ± 0.0
Pro
4.109ProAla: 4.109 ± 0.59
0.44ProCys: 0.44 ± 0.254
3.302ProAsp: 3.302 ± 0.506
4.109ProGlu: 4.109 ± 0.806
0.807ProPhe: 0.807 ± 0.284
3.229ProGly: 3.229 ± 0.429
0.954ProHis: 0.954 ± 0.31
0.954ProIle: 0.954 ± 0.346
1.614ProLys: 1.614 ± 0.398
2.642ProLeu: 2.642 ± 0.605
0.881ProMet: 0.881 ± 0.284
1.321ProAsn: 1.321 ± 0.363
1.761ProPro: 1.761 ± 0.456
0.881ProGln: 0.881 ± 0.221
1.908ProArg: 1.908 ± 0.396
2.862ProSer: 2.862 ± 0.518
1.981ProThr: 1.981 ± 0.407
4.183ProVal: 4.183 ± 0.607
0.587ProTrp: 0.587 ± 0.224
1.321ProTyr: 1.321 ± 0.339
0.0ProXaa: 0.0 ± 0.0
Gln
4.623GlnAla: 4.623 ± 1.036
0.734GlnCys: 0.734 ± 0.215
1.247GlnAsp: 1.247 ± 0.278
2.788GlnGlu: 2.788 ± 0.46
1.027GlnPhe: 1.027 ± 0.256
1.981GlnGly: 1.981 ± 0.337
1.027GlnHis: 1.027 ± 0.235
2.421GlnIle: 2.421 ± 0.633
2.275GlnLys: 2.275 ± 0.456
4.109GlnLeu: 4.109 ± 0.693
1.468GlnMet: 1.468 ± 0.3
1.101GlnAsn: 1.101 ± 0.352
1.541GlnPro: 1.541 ± 0.366
2.788GlnGln: 2.788 ± 0.993
2.788GlnArg: 2.788 ± 0.662
2.715GlnSer: 2.715 ± 0.414
2.495GlnThr: 2.495 ± 0.373
3.082GlnVal: 3.082 ± 0.555
0.881GlnTrp: 0.881 ± 0.273
1.247GlnTyr: 1.247 ± 0.249
0.0GlnXaa: 0.0 ± 0.0
Arg
4.696ArgAla: 4.696 ± 0.557
1.027ArgCys: 1.027 ± 0.317
2.935ArgAsp: 2.935 ± 0.543
3.889ArgGlu: 3.889 ± 0.488
1.981ArgPhe: 1.981 ± 0.324
3.522ArgGly: 3.522 ± 0.42
1.614ArgHis: 1.614 ± 0.316
2.862ArgIle: 2.862 ± 0.427
3.742ArgLys: 3.742 ± 0.462
6.164ArgLeu: 6.164 ± 0.806
2.642ArgMet: 2.642 ± 0.463
2.421ArgAsn: 2.421 ± 0.42
2.201ArgPro: 2.201 ± 0.5
3.816ArgGln: 3.816 ± 1.009
4.696ArgArg: 4.696 ± 0.887
2.715ArgSer: 2.715 ± 0.364
3.302ArgThr: 3.302 ± 0.629
4.256ArgVal: 4.256 ± 0.669
1.174ArgTrp: 1.174 ± 0.411
2.715ArgTyr: 2.715 ± 0.343
0.0ArgXaa: 0.0 ± 0.0
Ser
7.998SerAla: 7.998 ± 1.411
0.367SerCys: 0.367 ± 0.185
5.21SerAsp: 5.21 ± 0.649
4.77SerGlu: 4.77 ± 0.846
2.275SerPhe: 2.275 ± 0.376
7.998SerGly: 7.998 ± 0.768
0.807SerHis: 0.807 ± 0.287
2.568SerIle: 2.568 ± 0.423
3.229SerLys: 3.229 ± 0.503
5.577SerLeu: 5.577 ± 0.562
1.321SerMet: 1.321 ± 0.29
1.981SerAsn: 1.981 ± 0.338
2.201SerPro: 2.201 ± 0.432
2.715SerGln: 2.715 ± 0.35
3.816SerArg: 3.816 ± 0.516
4.843SerSer: 4.843 ± 0.848
3.816SerThr: 3.816 ± 0.664
6.531SerVal: 6.531 ± 0.72
0.587SerTrp: 0.587 ± 0.205
1.688SerTyr: 1.688 ± 0.346
0.0SerXaa: 0.0 ± 0.0
Thr
6.384ThrAla: 6.384 ± 0.957
0.294ThrCys: 0.294 ± 0.157
3.669ThrAsp: 3.669 ± 0.608
3.596ThrGlu: 3.596 ± 0.46
2.495ThrPhe: 2.495 ± 0.362
4.183ThrGly: 4.183 ± 0.559
1.321ThrHis: 1.321 ± 0.295
2.788ThrIle: 2.788 ± 0.43
2.715ThrLys: 2.715 ± 0.521
4.623ThrLeu: 4.623 ± 0.609
0.881ThrMet: 0.881 ± 0.299
2.128ThrAsn: 2.128 ± 0.601
3.009ThrPro: 3.009 ± 0.688
2.275ThrGln: 2.275 ± 0.521
3.742ThrArg: 3.742 ± 0.545
3.889ThrSer: 3.889 ± 0.652
3.816ThrThr: 3.816 ± 0.655
4.843ThrVal: 4.843 ± 0.831
0.881ThrTrp: 0.881 ± 0.219
1.908ThrTyr: 1.908 ± 0.335
0.0ThrXaa: 0.0 ± 0.0
Val
5.944ValAla: 5.944 ± 0.832
0.807ValCys: 0.807 ± 0.291
3.009ValAsp: 3.009 ± 0.487
3.669ValGlu: 3.669 ± 0.663
2.421ValPhe: 2.421 ± 0.485
4.696ValGly: 4.696 ± 0.599
0.807ValHis: 0.807 ± 0.235
4.696ValIle: 4.696 ± 0.65
4.77ValLys: 4.77 ± 0.623
7.118ValLeu: 7.118 ± 0.534
1.614ValMet: 1.614 ± 0.311
3.082ValAsn: 3.082 ± 0.482
2.862ValPro: 2.862 ± 0.619
2.935ValGln: 2.935 ± 0.578
3.669ValArg: 3.669 ± 0.607
5.797ValSer: 5.797 ± 0.67
4.916ValThr: 4.916 ± 0.645
3.816ValVal: 3.816 ± 0.607
1.468ValTrp: 1.468 ± 0.387
1.688ValTyr: 1.688 ± 0.349
0.0ValXaa: 0.0 ± 0.0
Trp
1.468TrpAla: 1.468 ± 0.341
0.147TrpCys: 0.147 ± 0.094
0.807TrpAsp: 0.807 ± 0.276
0.66TrpGlu: 0.66 ± 0.202
0.66TrpPhe: 0.66 ± 0.219
1.027TrpGly: 1.027 ± 0.252
0.44TrpHis: 0.44 ± 0.229
0.807TrpIle: 0.807 ± 0.207
1.027TrpLys: 1.027 ± 0.313
2.495TrpLeu: 2.495 ± 0.553
0.587TrpMet: 0.587 ± 0.19
0.514TrpAsn: 0.514 ± 0.175
0.66TrpPro: 0.66 ± 0.299
0.587TrpGln: 0.587 ± 0.185
1.101TrpArg: 1.101 ± 0.344
1.101TrpSer: 1.101 ± 0.244
0.954TrpThr: 0.954 ± 0.238
0.954TrpVal: 0.954 ± 0.315
0.44TrpTrp: 0.44 ± 0.241
1.027TrpTyr: 1.027 ± 0.275
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.715TyrAla: 2.715 ± 0.504
0.294TyrCys: 0.294 ± 0.156
1.908TyrAsp: 1.908 ± 0.287
1.394TyrGlu: 1.394 ± 0.344
1.101TyrPhe: 1.101 ± 0.328
1.834TyrGly: 1.834 ± 0.419
0.44TyrHis: 0.44 ± 0.174
1.468TyrIle: 1.468 ± 0.312
1.614TyrLys: 1.614 ± 0.383
3.302TyrLeu: 3.302 ± 0.352
0.22TyrMet: 0.22 ± 0.111
1.174TyrAsn: 1.174 ± 0.264
0.807TyrPro: 0.807 ± 0.211
1.688TyrGln: 1.688 ± 0.348
1.834TyrArg: 1.834 ± 0.342
3.009TyrSer: 3.009 ± 0.48
1.688TyrThr: 1.688 ± 0.336
1.468TyrVal: 1.468 ± 0.331
0.514TyrTrp: 0.514 ± 0.196
0.66TyrTyr: 0.66 ± 0.226
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 58 proteins (13629 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski