bacterium HR31

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 7.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 927 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H5ZR23|A0A2H5ZR23_9BACT Cell division ATP-binding protein FtsE OS=bacterium HR31 OX=2035426 GN=ftsE PE=3 SV=1
MM1 pKa = 7.3TVPFNRR7 pKa = 11.84PCPQCGGEE15 pKa = 4.15MEE17 pKa = 5.18LLASDD22 pKa = 3.79EE23 pKa = 4.34LGAVVVYY30 pKa = 9.61EE31 pKa = 4.25CAEE34 pKa = 4.01CGYY37 pKa = 8.89QAKK40 pKa = 10.02EE41 pKa = 3.73RR42 pKa = 11.84VEE44 pKa = 4.34EE45 pKa = 3.99VLEE48 pKa = 4.05PAEE51 pKa = 4.15EE52 pKa = 4.51DD53 pKa = 3.63EE54 pKa = 5.09VALQPEE60 pKa = 4.52EE61 pKa = 4.24EE62 pKa = 4.36EE63 pKa = 4.2EE64 pKa = 4.35EE65 pKa = 4.19EE66 pKa = 4.3FPEE69 pKa = 4.27EE70 pKa = 4.14EE71 pKa = 3.88EE72 pKa = 4.8DD73 pKa = 5.05EE74 pKa = 4.29EE75 pKa = 5.59FEE77 pKa = 4.77DD78 pKa = 5.36SLWEE82 pKa = 4.29DD83 pKa = 3.6EE84 pKa = 4.99EE85 pKa = 4.56EE86 pKa = 5.83EE87 pKa = 3.92EE88 pKa = 5.22DD89 pKa = 3.28AWRR92 pKa = 4.28

Molecular weight: 10.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H5ZSW6|A0A2H5ZSW6_9BACT Cupin_2 domain-containing protein OS=bacterium HR31 OX=2035426 GN=HRbin31_00832 PE=4 SV=1
MM1 pKa = 7.43GVTKK5 pKa = 10.02PKK7 pKa = 9.99RR8 pKa = 11.84SSRR11 pKa = 11.84YY12 pKa = 8.58GSAPVARR19 pKa = 11.84MPHH22 pKa = 5.58RR23 pKa = 11.84TPPAAPTPALRR34 pKa = 11.84AASRR38 pKa = 11.84VGAPARR44 pKa = 11.84RR45 pKa = 11.84SSASAVAALASPTSRR60 pKa = 11.84PAYY63 pKa = 9.24ARR65 pKa = 11.84SVRR68 pKa = 11.84RR69 pKa = 11.84WIRR72 pKa = 11.84RR73 pKa = 11.84ALNTPTAAPAYY84 pKa = 7.72ATATRR89 pKa = 11.84RR90 pKa = 11.84RR91 pKa = 11.84SWVPSVPARR100 pKa = 11.84TASAPAAKK108 pKa = 9.81SASRR112 pKa = 11.84GFTSTASRR120 pKa = 11.84MATAAVGTSSGWIVAATRR138 pKa = 11.84RR139 pKa = 11.84VPAAPSHH146 pKa = 5.31TRR148 pKa = 11.84SPVSSRR154 pKa = 11.84SLGLLHH160 pKa = 6.57TSGWKK165 pKa = 6.88WARR168 pKa = 11.84YY169 pKa = 8.96APRR172 pKa = 11.84TATRR176 pKa = 11.84AAASTRR182 pKa = 11.84VPTSLLHH189 pKa = 4.84GHH191 pKa = 6.48GPVASPRR198 pKa = 11.84ACSLRR203 pKa = 11.84ICSGVTTSPRR213 pKa = 11.84RR214 pKa = 11.84TTTSSFRR221 pKa = 11.84TRR223 pKa = 11.84TRR225 pKa = 11.84TGSTPARR232 pKa = 11.84AASRR236 pKa = 11.84ARR238 pKa = 11.84RR239 pKa = 11.84VCSSSTTRR247 pKa = 11.84LGRR250 pKa = 11.84RR251 pKa = 11.84ARR253 pKa = 11.84SATHH257 pKa = 7.02LLNARR262 pKa = 11.84AGPLTPFSRR271 pKa = 11.84RR272 pKa = 11.84IGSSCWRR279 pKa = 11.84ALDD282 pKa = 3.6RR283 pKa = 11.84SSGLRR288 pKa = 11.84GRR290 pKa = 11.84AIKK293 pKa = 10.4RR294 pKa = 3.39

Molecular weight: 31.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

927

0

927

267116

34

1041

288.2

31.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.102 ± 0.097

0.891 ± 0.026

4.513 ± 0.055

6.808 ± 0.093

3.038 ± 0.052

9.036 ± 0.091

2.21 ± 0.062

2.72 ± 0.062

1.744 ± 0.046

11.203 ± 0.114

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.647 ± 0.036

1.507 ± 0.033

6.152 ± 0.083

3.321 ± 0.072

10.307 ± 0.099

4.039 ± 0.054

4.394 ± 0.056

10.591 ± 0.081

1.471 ± 0.04

2.307 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski