Amino acid dipepetide frequency for Mycobacterium phage Nivrat

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.098AlaAla: 13.098 ± 1.947
0.928AlaCys: 0.928 ± 0.251
6.877AlaAsp: 6.877 ± 0.608
6.986AlaGlu: 6.986 ± 0.967
2.62AlaPhe: 2.62 ± 0.346
10.042AlaGly: 10.042 ± 1.201
2.292AlaHis: 2.292 ± 0.451
4.093AlaIle: 4.093 ± 0.597
4.475AlaLys: 4.475 ± 0.502
8.459AlaLeu: 8.459 ± 0.874
2.074AlaMet: 2.074 ± 0.367
2.838AlaAsn: 2.838 ± 0.324
4.421AlaPro: 4.421 ± 0.523
3.438AlaGln: 3.438 ± 0.471
6.877AlaArg: 6.877 ± 0.85
5.567AlaSer: 5.567 ± 0.584
6.167AlaThr: 6.167 ± 0.495
7.04AlaVal: 7.04 ± 0.662
2.238AlaTrp: 2.238 ± 0.387
2.183AlaTyr: 2.183 ± 0.376
0.0AlaXaa: 0.0 ± 0.0
Cys
0.819CysAla: 0.819 ± 0.247
0.055CysCys: 0.055 ± 0.059
1.201CysAsp: 1.201 ± 0.299
1.31CysGlu: 1.31 ± 0.28
0.164CysPhe: 0.164 ± 0.11
1.528CysGly: 1.528 ± 0.371
0.273CysHis: 0.273 ± 0.121
0.109CysIle: 0.109 ± 0.072
0.6CysLys: 0.6 ± 0.195
0.655CysLeu: 0.655 ± 0.207
0.218CysMet: 0.218 ± 0.102
0.6CysAsn: 0.6 ± 0.192
1.474CysPro: 1.474 ± 0.372
0.273CysGln: 0.273 ± 0.127
0.655CysArg: 0.655 ± 0.21
0.709CysSer: 0.709 ± 0.244
0.764CysThr: 0.764 ± 0.207
0.764CysVal: 0.764 ± 0.195
0.382CysTrp: 0.382 ± 0.126
0.164CysTyr: 0.164 ± 0.079
0.0CysXaa: 0.0 ± 0.0
Asp
6.877AspAla: 6.877 ± 0.727
0.873AspCys: 0.873 ± 0.221
4.366AspAsp: 4.366 ± 0.581
3.82AspGlu: 3.82 ± 0.494
1.746AspPhe: 1.746 ± 0.293
6.44AspGly: 6.44 ± 0.675
0.982AspHis: 0.982 ± 0.191
2.456AspIle: 2.456 ± 0.368
1.856AspLys: 1.856 ± 0.38
5.894AspLeu: 5.894 ± 0.484
1.201AspMet: 1.201 ± 0.255
1.746AspAsn: 1.746 ± 0.333
4.803AspPro: 4.803 ± 0.542
2.729AspGln: 2.729 ± 0.4
5.021AspArg: 5.021 ± 0.537
3.384AspSer: 3.384 ± 0.512
3.984AspThr: 3.984 ± 0.497
4.748AspVal: 4.748 ± 0.528
1.419AspTrp: 1.419 ± 0.303
1.965AspTyr: 1.965 ± 0.35
0.0AspXaa: 0.0 ± 0.0
Glu
6.276GluAla: 6.276 ± 0.748
1.092GluCys: 1.092 ± 0.273
3.602GluAsp: 3.602 ± 0.394
3.056GluGlu: 3.056 ± 0.527
2.183GluPhe: 2.183 ± 0.355
3.384GluGly: 3.384 ± 0.359
1.801GluHis: 1.801 ± 0.412
1.965GluIle: 1.965 ± 0.347
2.019GluLys: 2.019 ± 0.291
6.276GluLeu: 6.276 ± 0.843
1.474GluMet: 1.474 ± 0.279
1.637GluAsn: 1.637 ± 0.26
3.22GluPro: 3.22 ± 0.459
2.729GluGln: 2.729 ± 0.369
5.294GluArg: 5.294 ± 0.598
3.002GluSer: 3.002 ± 0.509
4.694GluThr: 4.694 ± 0.542
4.039GluVal: 4.039 ± 0.413
1.528GluTrp: 1.528 ± 0.272
1.91GluTyr: 1.91 ± 0.333
0.0GluXaa: 0.0 ± 0.0
Phe
2.565PheAla: 2.565 ± 0.421
0.164PheCys: 0.164 ± 0.098
3.22PheAsp: 3.22 ± 0.55
1.637PheGlu: 1.637 ± 0.278
0.982PhePhe: 0.982 ± 0.259
3.056PheGly: 3.056 ± 0.697
0.6PheHis: 0.6 ± 0.191
1.364PheIle: 1.364 ± 0.436
0.982PheLys: 0.982 ± 0.246
1.692PheLeu: 1.692 ± 0.271
0.709PheMet: 0.709 ± 0.157
1.201PheAsn: 1.201 ± 0.313
1.201PhePro: 1.201 ± 0.246
1.037PheGln: 1.037 ± 0.309
1.692PheArg: 1.692 ± 0.297
1.364PheSer: 1.364 ± 0.282
2.292PheThr: 2.292 ± 0.34
2.074PheVal: 2.074 ± 0.28
0.655PheTrp: 0.655 ± 0.189
0.873PheTyr: 0.873 ± 0.284
0.0PheXaa: 0.0 ± 0.0
Gly
8.95GlyAla: 8.95 ± 1.157
1.146GlyCys: 1.146 ± 0.273
5.021GlyAsp: 5.021 ± 0.47
5.185GlyGlu: 5.185 ± 0.533
2.565GlyPhe: 2.565 ± 0.343
10.206GlyGly: 10.206 ± 2.095
1.965GlyHis: 1.965 ± 0.337
3.438GlyIle: 3.438 ± 0.596
2.401GlyLys: 2.401 ± 0.314
6.003GlyLeu: 6.003 ± 0.563
2.511GlyMet: 2.511 ± 0.547
3.111GlyAsn: 3.111 ± 0.429
4.093GlyPro: 4.093 ± 0.657
2.238GlyGln: 2.238 ± 0.512
5.403GlyArg: 5.403 ± 0.597
6.822GlySer: 6.822 ± 0.95
6.385GlyThr: 6.385 ± 0.856
5.894GlyVal: 5.894 ± 0.595
2.128GlyTrp: 2.128 ± 0.332
2.074GlyTyr: 2.074 ± 0.355
0.0GlyXaa: 0.0 ± 0.0
His
1.746HisAla: 1.746 ± 0.339
0.327HisCys: 0.327 ± 0.162
1.092HisAsp: 1.092 ± 0.234
1.037HisGlu: 1.037 ± 0.296
0.546HisPhe: 0.546 ± 0.148
1.746HisGly: 1.746 ± 0.322
0.928HisHis: 0.928 ± 0.274
1.31HisIle: 1.31 ± 0.327
1.092HisLys: 1.092 ± 0.282
1.364HisLeu: 1.364 ± 0.228
0.382HisMet: 0.382 ± 0.122
0.928HisAsn: 0.928 ± 0.167
1.255HisPro: 1.255 ± 0.253
0.709HisGln: 0.709 ± 0.193
1.856HisArg: 1.856 ± 0.367
1.146HisSer: 1.146 ± 0.231
1.419HisThr: 1.419 ± 0.336
1.746HisVal: 1.746 ± 0.428
0.382HisTrp: 0.382 ± 0.129
0.873HisTyr: 0.873 ± 0.202
0.0HisXaa: 0.0 ± 0.0
Ile
5.021IleAla: 5.021 ± 0.543
0.709IleCys: 0.709 ± 0.236
3.493IleAsp: 3.493 ± 0.463
3.384IleGlu: 3.384 ± 0.342
0.6IlePhe: 0.6 ± 0.172
3.984IleGly: 3.984 ± 0.485
1.528IleHis: 1.528 ± 0.332
1.583IleIle: 1.583 ± 0.255
1.092IleLys: 1.092 ± 0.259
2.074IleLeu: 2.074 ± 0.35
0.382IleMet: 0.382 ± 0.12
1.419IleAsn: 1.419 ± 0.279
3.002IlePro: 3.002 ± 0.382
1.474IleGln: 1.474 ± 0.243
2.456IleArg: 2.456 ± 0.395
2.238IleSer: 2.238 ± 0.409
3.766IleThr: 3.766 ± 0.444
3.002IleVal: 3.002 ± 0.388
0.982IleTrp: 0.982 ± 0.264
0.819IleTyr: 0.819 ± 0.194
0.0IleXaa: 0.0 ± 0.0
Lys
3.984LysAla: 3.984 ± 0.581
0.6LysCys: 0.6 ± 0.185
1.746LysAsp: 1.746 ± 0.263
1.474LysGlu: 1.474 ± 0.325
1.419LysPhe: 1.419 ± 0.237
2.347LysGly: 2.347 ± 0.485
1.092LysHis: 1.092 ± 0.275
0.928LysIle: 0.928 ± 0.205
1.419LysLys: 1.419 ± 0.339
2.511LysLeu: 2.511 ± 0.47
0.709LysMet: 0.709 ± 0.169
1.037LysAsn: 1.037 ± 0.23
2.565LysPro: 2.565 ± 0.383
1.364LysGln: 1.364 ± 0.273
2.183LysArg: 2.183 ± 0.406
2.401LysSer: 2.401 ± 0.339
2.019LysThr: 2.019 ± 0.324
2.401LysVal: 2.401 ± 0.407
0.928LysTrp: 0.928 ± 0.2
0.819LysTyr: 0.819 ± 0.232
0.0LysXaa: 0.0 ± 0.0
Leu
8.241LeuAla: 8.241 ± 0.945
0.873LeuCys: 0.873 ± 0.241
4.312LeuAsp: 4.312 ± 0.521
4.857LeuGlu: 4.857 ± 0.513
2.511LeuPhe: 2.511 ± 0.45
5.731LeuGly: 5.731 ± 0.608
0.928LeuHis: 0.928 ± 0.221
3.22LeuIle: 3.22 ± 0.445
2.238LeuLys: 2.238 ± 0.41
4.966LeuLeu: 4.966 ± 0.545
1.801LeuMet: 1.801 ± 0.372
2.729LeuAsn: 2.729 ± 0.372
5.348LeuPro: 5.348 ± 0.645
2.838LeuGln: 2.838 ± 0.428
5.021LeuArg: 5.021 ± 0.608
4.475LeuSer: 4.475 ± 0.536
5.348LeuThr: 5.348 ± 0.49
5.294LeuVal: 5.294 ± 0.528
1.146LeuTrp: 1.146 ± 0.232
2.183LeuTyr: 2.183 ± 0.365
0.0LeuXaa: 0.0 ± 0.0
Met
2.128MetAla: 2.128 ± 0.373
0.273MetCys: 0.273 ± 0.239
1.583MetAsp: 1.583 ± 0.272
0.982MetGlu: 0.982 ± 0.215
0.655MetPhe: 0.655 ± 0.196
1.419MetGly: 1.419 ± 0.246
0.164MetHis: 0.164 ± 0.089
0.819MetIle: 0.819 ± 0.219
0.491MetLys: 0.491 ± 0.161
1.801MetLeu: 1.801 ± 0.31
0.437MetMet: 0.437 ± 0.202
0.655MetAsn: 0.655 ± 0.17
1.364MetPro: 1.364 ± 0.27
0.709MetGln: 0.709 ± 0.188
1.856MetArg: 1.856 ± 0.343
3.384MetSer: 3.384 ± 0.428
2.238MetThr: 2.238 ± 0.323
1.201MetVal: 1.201 ± 0.318
0.491MetTrp: 0.491 ± 0.175
0.382MetTyr: 0.382 ± 0.128
0.0MetXaa: 0.0 ± 0.0
Asn
3.165AsnAla: 3.165 ± 0.373
0.437AsnCys: 0.437 ± 0.149
1.692AsnAsp: 1.692 ± 0.34
1.583AsnGlu: 1.583 ± 0.354
0.709AsnPhe: 0.709 ± 0.272
3.602AsnGly: 3.602 ± 0.524
0.764AsnHis: 0.764 ± 0.179
1.637AsnIle: 1.637 ± 0.505
0.928AsnLys: 0.928 ± 0.235
2.292AsnLeu: 2.292 ± 0.384
0.873AsnMet: 0.873 ± 0.223
1.746AsnAsn: 1.746 ± 0.291
2.674AsnPro: 2.674 ± 0.353
1.037AsnGln: 1.037 ± 0.303
2.292AsnArg: 2.292 ± 0.419
1.201AsnSer: 1.201 ± 0.293
2.183AsnThr: 2.183 ± 0.335
2.074AsnVal: 2.074 ± 0.299
0.709AsnTrp: 0.709 ± 0.206
0.709AsnTyr: 0.709 ± 0.183
0.0AsnXaa: 0.0 ± 0.0
Pro
4.966ProAla: 4.966 ± 0.546
0.873ProCys: 0.873 ± 0.201
4.093ProAsp: 4.093 ± 0.492
4.257ProGlu: 4.257 ± 0.48
1.965ProPhe: 1.965 ± 0.355
6.276ProGly: 6.276 ± 0.69
1.474ProHis: 1.474 ± 0.305
1.692ProIle: 1.692 ± 0.279
1.965ProLys: 1.965 ± 0.362
4.803ProLeu: 4.803 ± 0.538
1.637ProMet: 1.637 ± 0.349
2.074ProAsn: 2.074 ± 0.366
4.202ProPro: 4.202 ± 0.528
2.62ProGln: 2.62 ± 0.379
2.838ProArg: 2.838 ± 0.453
3.657ProSer: 3.657 ± 0.402
3.711ProThr: 3.711 ± 0.492
4.366ProVal: 4.366 ± 0.469
1.092ProTrp: 1.092 ± 0.206
1.419ProTyr: 1.419 ± 0.283
0.0ProXaa: 0.0 ± 0.0
Gln
4.421GlnAla: 4.421 ± 0.483
0.491GlnCys: 0.491 ± 0.173
1.474GlnAsp: 1.474 ± 0.245
1.528GlnGlu: 1.528 ± 0.334
1.092GlnPhe: 1.092 ± 0.232
2.62GlnGly: 2.62 ± 0.446
0.982GlnHis: 0.982 ± 0.225
2.074GlnIle: 2.074 ± 0.391
1.419GlnLys: 1.419 ± 0.253
3.002GlnLeu: 3.002 ± 0.398
0.819GlnMet: 0.819 ± 0.194
0.764GlnAsn: 0.764 ± 0.233
2.456GlnPro: 2.456 ± 0.369
1.146GlnGln: 1.146 ± 0.256
2.729GlnArg: 2.729 ± 0.368
2.074GlnSer: 2.074 ± 0.364
1.801GlnThr: 1.801 ± 0.243
2.401GlnVal: 2.401 ± 0.352
0.546GlnTrp: 0.546 ± 0.178
0.764GlnTyr: 0.764 ± 0.25
0.0GlnXaa: 0.0 ± 0.0
Arg
6.495ArgAla: 6.495 ± 0.745
1.092ArgCys: 1.092 ± 0.358
5.076ArgAsp: 5.076 ± 0.672
5.403ArgGlu: 5.403 ± 0.714
2.238ArgPhe: 2.238 ± 0.392
3.984ArgGly: 3.984 ± 0.45
1.146ArgHis: 1.146 ± 0.283
4.53ArgIle: 4.53 ± 0.671
2.62ArgLys: 2.62 ± 0.445
5.13ArgLeu: 5.13 ± 0.563
2.347ArgMet: 2.347 ± 0.356
2.074ArgAsn: 2.074 ± 0.409
3.329ArgPro: 3.329 ± 0.395
1.637ArgGln: 1.637 ± 0.339
5.567ArgArg: 5.567 ± 0.846
3.766ArgSer: 3.766 ± 0.444
3.657ArgThr: 3.657 ± 0.626
4.912ArgVal: 4.912 ± 0.696
1.856ArgTrp: 1.856 ± 0.375
2.183ArgTyr: 2.183 ± 0.352
0.0ArgXaa: 0.0 ± 0.0
Ser
5.621SerAla: 5.621 ± 0.89
0.491SerCys: 0.491 ± 0.2
4.475SerAsp: 4.475 ± 0.511
2.783SerGlu: 2.783 ± 0.34
1.965SerPhe: 1.965 ± 0.369
6.604SerGly: 6.604 ± 0.96
0.928SerHis: 0.928 ± 0.194
3.165SerIle: 3.165 ± 0.434
2.238SerLys: 2.238 ± 0.394
3.766SerLeu: 3.766 ± 0.431
1.583SerMet: 1.583 ± 0.309
2.238SerAsn: 2.238 ± 0.32
3.438SerPro: 3.438 ± 0.391
1.801SerGln: 1.801 ± 0.278
3.275SerArg: 3.275 ± 0.437
3.766SerSer: 3.766 ± 0.68
3.657SerThr: 3.657 ± 0.535
5.021SerVal: 5.021 ± 0.669
1.255SerTrp: 1.255 ± 0.258
1.474SerTyr: 1.474 ± 0.283
0.0SerXaa: 0.0 ± 0.0
Thr
6.331ThrAla: 6.331 ± 0.567
0.6ThrCys: 0.6 ± 0.224
4.312ThrAsp: 4.312 ± 0.53
3.875ThrGlu: 3.875 ± 0.477
1.801ThrPhe: 1.801 ± 0.328
6.276ThrGly: 6.276 ± 0.581
1.801ThrHis: 1.801 ± 0.332
3.547ThrIle: 3.547 ± 0.484
2.019ThrLys: 2.019 ± 0.334
3.929ThrLeu: 3.929 ± 0.473
1.364ThrMet: 1.364 ± 0.253
1.91ThrAsn: 1.91 ± 0.427
4.53ThrPro: 4.53 ± 0.48
2.074ThrGln: 2.074 ± 0.335
4.584ThrArg: 4.584 ± 0.534
4.093ThrSer: 4.093 ± 0.535
5.13ThrThr: 5.13 ± 0.752
6.331ThrVal: 6.331 ± 0.614
1.201ThrTrp: 1.201 ± 0.262
1.856ThrTyr: 1.856 ± 0.334
0.0ThrXaa: 0.0 ± 0.0
Val
7.532ValAla: 7.532 ± 0.669
1.146ValCys: 1.146 ± 0.222
5.512ValAsp: 5.512 ± 0.621
4.803ValGlu: 4.803 ± 0.537
2.347ValPhe: 2.347 ± 0.414
5.512ValGly: 5.512 ± 0.586
1.364ValHis: 1.364 ± 0.261
2.838ValIle: 2.838 ± 0.385
2.401ValLys: 2.401 ± 0.382
5.676ValLeu: 5.676 ± 0.619
1.364ValMet: 1.364 ± 0.298
2.128ValAsn: 2.128 ± 0.347
3.82ValPro: 3.82 ± 0.394
2.783ValGln: 2.783 ± 0.314
4.966ValArg: 4.966 ± 0.633
4.475ValSer: 4.475 ± 0.619
5.076ValThr: 5.076 ± 0.508
6.604ValVal: 6.604 ± 0.81
1.965ValTrp: 1.965 ± 0.331
1.419ValTyr: 1.419 ± 0.299
0.0ValXaa: 0.0 ± 0.0
Trp
2.128TrpAla: 2.128 ± 0.34
0.382TrpCys: 0.382 ± 0.142
1.201TrpAsp: 1.201 ± 0.306
1.146TrpGlu: 1.146 ± 0.294
0.655TrpPhe: 0.655 ± 0.163
0.928TrpGly: 0.928 ± 0.215
0.655TrpHis: 0.655 ± 0.218
1.092TrpIle: 1.092 ± 0.219
0.982TrpLys: 0.982 ± 0.199
1.856TrpLeu: 1.856 ± 0.38
0.928TrpMet: 0.928 ± 0.214
0.709TrpAsn: 0.709 ± 0.226
0.982TrpPro: 0.982 ± 0.319
1.037TrpGln: 1.037 ± 0.278
2.401TrpArg: 2.401 ± 0.401
1.037TrpSer: 1.037 ± 0.236
1.419TrpThr: 1.419 ± 0.244
1.474TrpVal: 1.474 ± 0.4
0.819TrpTrp: 0.819 ± 0.218
0.491TrpTyr: 0.491 ± 0.172
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.401TyrAla: 2.401 ± 0.388
0.164TyrCys: 0.164 ± 0.093
1.965TyrAsp: 1.965 ± 0.378
2.074TyrGlu: 2.074 ± 0.329
0.6TyrPhe: 0.6 ± 0.203
1.856TyrGly: 1.856 ± 0.361
0.218TyrHis: 0.218 ± 0.102
1.146TyrIle: 1.146 ± 0.202
0.709TyrLys: 0.709 ± 0.185
1.91TyrLeu: 1.91 ± 0.383
0.164TyrMet: 0.164 ± 0.099
0.764TyrAsn: 0.764 ± 0.229
1.801TyrPro: 1.801 ± 0.323
0.928TyrGln: 0.928 ± 0.215
2.074TyrArg: 2.074 ± 0.408
0.982TyrSer: 0.982 ± 0.255
1.856TyrThr: 1.856 ± 0.272
2.347TyrVal: 2.347 ± 0.378
0.6TyrTrp: 0.6 ± 0.175
0.6TyrTyr: 0.6 ± 0.189
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 103 proteins (18324 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski