Gordonia phage Pupper

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 233 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y6EKV8|A0A4Y6EKV8_9CAUD Uncharacterized protein OS=Gordonia phage Pupper OX=2571249 GN=189 PE=4 SV=1
MM1 pKa = 7.9AGRR4 pKa = 11.84FDD6 pKa = 4.13YY7 pKa = 11.4SHH9 pKa = 7.81DD10 pKa = 3.88DD11 pKa = 4.16SIDD14 pKa = 3.24SHH16 pKa = 7.15YY17 pKa = 10.76LTGPVSDD24 pKa = 5.18LAPSNYY30 pKa = 10.07EE31 pKa = 3.38EE32 pKa = 5.33DD33 pKa = 4.0AVHH36 pKa = 7.44DD37 pKa = 5.05DD38 pKa = 4.14EE39 pKa = 5.43PADD42 pKa = 4.11DD43 pKa = 4.75GGYY46 pKa = 7.82TGPMAKK52 pKa = 10.26DD53 pKa = 3.1PWLPGPSGPAPFF65 pKa = 4.83

Molecular weight: 6.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y6ETM2|A0A4Y6ETM2_9CAUD RuvC-like resolvase OS=Gordonia phage Pupper OX=2571249 GN=178 PE=4 SV=1
MM1 pKa = 6.9QVSGKK6 pKa = 9.19SVPVRR11 pKa = 11.84AHH13 pKa = 4.71VASYY17 pKa = 10.68RR18 pKa = 11.84IFHH21 pKa = 6.59GPTGGLHH28 pKa = 5.92VLHH31 pKa = 6.72SCDD34 pKa = 5.06RR35 pKa = 11.84PICVQPAHH43 pKa = 6.94LSLGDD48 pKa = 3.62HH49 pKa = 6.32VKK51 pKa = 11.02NMAEE55 pKa = 3.83KK56 pKa = 10.18QEE58 pKa = 4.13RR59 pKa = 11.84GRR61 pKa = 11.84TARR64 pKa = 11.84GEE66 pKa = 4.03RR67 pKa = 11.84QGAAKK72 pKa = 8.91LTEE75 pKa = 4.32DD76 pKa = 3.35AVLEE80 pKa = 4.1IRR82 pKa = 11.84ASSSSHH88 pKa = 6.19ASLAVLYY95 pKa = 9.61RR96 pKa = 11.84VSPSTIAQVRR106 pKa = 11.84RR107 pKa = 11.84RR108 pKa = 11.84KK109 pKa = 7.59TWTHH113 pKa = 4.78II114 pKa = 3.46

Molecular weight: 12.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

233

0

233

48016

34

1837

206.1

22.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.797 ± 0.254

0.837 ± 0.084

7.079 ± 0.164

6.554 ± 0.304

3.091 ± 0.105

8.391 ± 0.352

2.199 ± 0.138

4.199 ± 0.127

3.663 ± 0.143

7.564 ± 0.199

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.301 ± 0.116

3.38 ± 0.151

5.604 ± 0.169

4.138 ± 0.137

6.721 ± 0.217

6.188 ± 0.159

6.4 ± 0.241

7.143 ± 0.163

1.779 ± 0.076

2.972 ± 0.094
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski