Proteome-pI 2.0 - Proteome Isoelectric Point Database

Arcanobacterium haemolyticum (strain ATCC 9345 / DSM 20595 / NBRC 15585 / NCTC 8452 / 11018)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Actinomycetales; Actinomycetaceae; Arcanobacterium; Arcanobacterium haemolyticum

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 1717 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|D7BKZ3|D7BKZ3_ARCHD 2-amino-3-ketobutyrate coenzyme A ligase OS=Arcanobacterium haemolyticum (strain ATCC 9345 / DSM 20595 / NBRC 15585 / NCTC 8452 / 11018) OX=644284 GN=kbl PE=3 SV=1

MM1 pKa = 7.25TSIEE5 pKa = 4.34TTHH8 pKa = 6.27EE9 pKa = 3.87WEE11 pKa = 4.02LWDD14 pKa = 4.13AEE16 pKa = 4.15EE17 pKa = 5.6LAVQLGGFTIPTPEE31 pKa = 3.76EE32 pKa = 3.68RR33 pKa = 11.84AAVQPGDD40 pKa = 3.23IVKK43 pKa = 10.44LVFGLTEE50 pKa = 4.05PEE52 pKa = 3.96GEE54 pKa = 4.13IAAEE58 pKa = 4.02RR59 pKa = 11.84MWVIVDD65 pKa = 3.6GMDD68 pKa = 2.95AAGYY72 pKa = 10.08IGTLDD77 pKa = 3.95SDD79 pKa = 4.04PEE81 pKa = 4.51YY82 pKa = 9.87ITSIEE87 pKa = 4.17AGDD90 pKa = 3.89EE91 pKa = 4.08VRR93 pKa = 11.84FDD95 pKa = 3.3ASHH98 pKa = 6.71IIEE101 pKa = 4.61VFDD104 pKa = 3.51EE105 pKa = 4.24AAYY108 pKa = 9.61EE109 pKa = 4.24AGSGGCGGNCNCSCGKK125 pKa = 10.19

Molecular weight: 13.38 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|D7BLJ4|D7BLJ4_ARCHD 5'-Nucleotidase domain protein OS=Arcanobacterium haemolyticum (strain ATCC 9345 / DSM 20595 / NBRC 15585 / NCTC 8452 / 11018) OX=644284 GN=Arch_0025 PE=3 SV=1

MM1 pKa = 6.23STKK4 pKa = 9.67RR5 pKa = 11.84TFQPNNRR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84AKK16 pKa = 9.4VHH18 pKa = 5.49GFRR21 pKa = 11.84KK22 pKa = 10.04RR23 pKa = 11.84MATRR27 pKa = 11.84AGRR30 pKa = 11.84AVLASRR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84KK39 pKa = 9.61GRR41 pKa = 11.84ARR43 pKa = 11.84LAAA46 pKa = 4.44

Molecular weight: 5.35 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
1717	0	1717	569835	32	1782	331.9	36.14

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
11.05 ± 0.088	0.732 ± 0.018	5.91 ± 0.054	6.064 ± 0.067	3.481 ± 0.032	7.992 ± 0.043	2.267 ± 0.03	5.789 ± 0.053	3.847 ± 0.069	9.284 ± 0.068

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.383 ± 0.027	3.057 ± 0.039	4.73 ± 0.038	3.154 ± 0.027	6.016 ± 0.06	6.096 ± 0.039	6.062 ± 0.043	8.296 ± 0.054	1.396 ± 0.025	2.393 ± 0.03

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski