Thermosphaera aggregans (strain DSM 11486 / M11TL)

Taxonomy: cellular organisms; Archaea; TACK group; Crenarchaeota; Thermoprotei; Desulfurococcales; Desulfurococcaceae; Thermosphaera; Thermosphaera aggregans

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1387 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D5U256|D5U256_THEAM 50S ribosomal protein L18Ae OS=Thermosphaera aggregans (strain DSM 11486 / M11TL) OX=633148 GN=rpl18a PE=3 SV=1
MM1 pKa = 7.25NKK3 pKa = 10.3SMIYY7 pKa = 8.02MLSMALVGLSVLAGLAMWTDD27 pKa = 3.52SLKK30 pKa = 10.98ANSYY34 pKa = 10.43VSTGEE39 pKa = 3.96LDD41 pKa = 3.27WEE43 pKa = 4.58IVSGPTIWLDD53 pKa = 3.42ACGLEE58 pKa = 4.24PGYY61 pKa = 11.78GMFKK65 pKa = 10.83GNDD68 pKa = 3.11WNATFLPMPGAAQLDD83 pKa = 4.06KK84 pKa = 11.39DD85 pKa = 4.17VGCTNVSLIDD95 pKa = 3.6SDD97 pKa = 4.86GDD99 pKa = 3.51GDD101 pKa = 4.6YY102 pKa = 10.71DD103 pKa = 3.92TMNVTLVNVYY113 pKa = 8.83PWYY116 pKa = 8.19YY117 pKa = 8.25THH119 pKa = 7.88IAFKK123 pKa = 10.28VHH125 pKa = 6.78NDD127 pKa = 2.9GTIPLKK133 pKa = 9.76IWRR136 pKa = 11.84VVFDD140 pKa = 2.92GHH142 pKa = 6.37EE143 pKa = 3.94YY144 pKa = 10.93YY145 pKa = 10.53EE146 pKa = 4.22INEE149 pKa = 4.48AEE151 pKa = 4.18LQQGVEE157 pKa = 3.78VDD159 pKa = 4.2LNGDD163 pKa = 3.82GQPDD167 pKa = 3.43ILVWWGDD174 pKa = 3.49NFGKK178 pKa = 9.91QLHH181 pKa = 6.67PCQSADD187 pKa = 2.82ISFDD191 pKa = 3.43LTILQTAPQGASLSFTIYY209 pKa = 10.39FDD211 pKa = 4.86AIAWNEE217 pKa = 4.21YY218 pKa = 7.19YY219 pKa = 10.15TPSTVTPIPDD229 pKa = 3.27KK230 pKa = 10.86EE231 pKa = 4.04

Molecular weight: 25.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D5U255|D5U255_THEAM Translation initiation factor 6 OS=Thermosphaera aggregans (strain DSM 11486 / M11TL) OX=633148 GN=eif6 PE=3 SV=1
MM1 pKa = 7.7ARR3 pKa = 11.84FKK5 pKa = 10.95HH6 pKa = 5.74LARR9 pKa = 11.84KK10 pKa = 9.13LRR12 pKa = 11.84LAAAEE17 pKa = 4.28KK18 pKa = 9.24TNKK21 pKa = 9.57PIPIWVSVKK30 pKa = 8.61TRR32 pKa = 11.84MRR34 pKa = 11.84VRR36 pKa = 11.84RR37 pKa = 11.84GFRR40 pKa = 11.84LRR42 pKa = 11.84NWRR45 pKa = 11.84RR46 pKa = 11.84SKK48 pKa = 10.85LKK50 pKa = 10.81NII52 pKa = 4.23

Molecular weight: 6.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1387

0

1387

397285

32

1385

286.4

32.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.797 ± 0.055

0.751 ± 0.022

4.305 ± 0.039

7.37 ± 0.076

3.898 ± 0.038

7.246 ± 0.055

1.637 ± 0.027

7.506 ± 0.053

6.312 ± 0.07

11.012 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.175 ± 0.027

3.356 ± 0.032

4.574 ± 0.041

1.875 ± 0.026

5.672 ± 0.054

6.377 ± 0.056

4.912 ± 0.059

8.896 ± 0.055

1.12 ± 0.026

4.209 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski