Candidatus Parvarchaeum acidophilus ARMAN-5
Average proteome isoelectric point is 7.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1002 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D6GVX7|D6GVX7_PARA5 Uncharacterized protein OS=Candidatus Parvarchaeum acidophilus ARMAN-5 OX=662762 GN=BJBARM5_0647 PE=4 SV=1
MM1 pKa = 7.92 ALMEE5 pKa = 4.65 MMAFLNAGILIVAFALLLVVLSFSVSQINSTSVFANSEE43 pKa = 4.03 AVATGIGTRR52 pKa = 11.84 LITSPNCFAYY62 pKa = 9.8 TNTITYY68 pKa = 10.11 YY69 pKa = 11.08 NSTLDD74 pKa = 3.42 ASGGPLYY81 pKa = 10.65 SSSYY85 pKa = 9.27 TEE87 pKa = 3.94 PGVINLNKK95 pKa = 10.01 FLEE98 pKa = 4.2 NDD100 pKa = 3.71 FLSCMQYY107 pKa = 10.42 IYY109 pKa = 10.84 FGGATDD115 pKa = 5.31 LPALQSDD122 pKa = 4.47 LAAATGVSLTLTDD135 pKa = 3.66 TQDD138 pKa = 3.31 PTEE141 pKa = 4.57 FGATGSLSLSNYY153 pKa = 8.34 NQFNFGASFQQTEE166 pKa = 3.97 AKK168 pKa = 9.43 IQSFAKK174 pKa = 9.77 DD175 pKa = 3.21 AQYY178 pKa = 11.81 AAMAASLAVSLPIMIATGGAVQFNLILAVGQQSTTEE214 pKa = 3.94 IAPQYY219 pKa = 10.45 ALANIFYY226 pKa = 10.45 SEE228 pKa = 4.05 STYY231 pKa = 10.82 TEE233 pKa = 4.18 SFPVIIQFTNANNQPTFQNSGVLTVDD259 pKa = 3.19 ITYY262 pKa = 9.7 GIPPYY267 pKa = 10.82 SS268 pKa = 3.28
Molecular weight: 28.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.906
IPC2_protein 3.706
IPC_protein 3.63
Toseland 3.439
ProMoST 3.795
Dawson 3.617
Bjellqvist 3.783
Wikipedia 3.554
Rodwell 3.465
Grimsley 3.35
Solomon 3.579
Lehninger 3.541
Nozaki 3.745
DTASelect 3.91
Thurlkill 3.503
EMBOSS 3.554
Sillero 3.745
Patrickios 0.006
IPC_peptide 3.579
IPC2_peptide 3.719
IPC2.peptide.svr19 3.7
Protein with the highest isoelectric point:
>tr|D6GUY5|D6GUY5_PARA5 Uncharacterized protein OS=Candidatus Parvarchaeum acidophilus ARMAN-5 OX=662762 GN=BJBARM5_0285 PE=4 SV=1
MM1 pKa = 7.9 AEE3 pKa = 4.01 RR4 pKa = 11.84 SNPVADD10 pKa = 4.13 KK11 pKa = 10.71 RR12 pKa = 11.84 LFYY15 pKa = 10.84 NVYY18 pKa = 9.79 VCRR21 pKa = 11.84 NCNTKK26 pKa = 9.16 MRR28 pKa = 11.84 ISSQKK33 pKa = 8.79 VLRR36 pKa = 11.84 NNKK39 pKa = 8.1 VRR41 pKa = 11.84 CRR43 pKa = 11.84 NCGSSALRR51 pKa = 11.84 PKK53 pKa = 10.49 HH54 pKa = 6.6 KK55 pKa = 9.98 EE56 pKa = 3.58 LRR58 pKa = 11.84 KK59 pKa = 10.36 LKK61 pKa = 10.44 VV62 pKa = 2.93
Molecular weight: 7.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.325
IPC2_protein 9.838
IPC_protein 10.613
Toseland 11.008
ProMoST 10.701
Dawson 11.067
Bjellqvist 10.774
Wikipedia 11.272
Rodwell 11.359
Grimsley 11.096
Solomon 11.213
Lehninger 11.184
Nozaki 10.994
DTASelect 10.76
Thurlkill 10.994
EMBOSS 11.403
Sillero 11.008
Patrickios 11.096
IPC_peptide 11.228
IPC2_peptide 9.999
IPC2.peptide.svr19 8.51
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1002
0
1002
265128
30
3776
264.6
29.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.746 ± 0.065
0.768 ± 0.029
5.042 ± 0.087
6.08 ± 0.115
4.825 ± 0.064
6.372 ± 0.083
1.25 ± 0.031
9.305 ± 0.103
8.675 ± 0.165
9.016 ± 0.096
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.33 ± 0.049
6.383 ± 0.111
3.317 ± 0.057
2.259 ± 0.042
3.466 ± 0.086
8.466 ± 0.188
5.211 ± 0.119
6.287 ± 0.059
0.717 ± 0.029
4.485 ± 0.073
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here