Escherichia phage HK446

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Hendrixvirinae; Kwaitsingvirus; Escherichia virus HK446

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K7P7P3|K7P7P3_9CAUD Late gene regulator Q OS=Escherichia phage HK446 OX=1147145 GN=HK446_054 PE=3 SV=1
MM1 pKa = 7.18TMDD4 pKa = 4.01YY5 pKa = 10.91SQLSDD10 pKa = 3.52FEE12 pKa = 5.23INVAVFEE19 pKa = 5.08AIHH22 pKa = 6.25NGSPDD27 pKa = 3.53YY28 pKa = 11.27KK29 pKa = 10.28EE30 pKa = 4.73GEE32 pKa = 3.99NGAMVFVSFEE42 pKa = 3.94GDD44 pKa = 3.22IVNGDD49 pKa = 3.41AVEE52 pKa = 4.33VEE54 pKa = 4.34VEE56 pKa = 3.87RR57 pKa = 11.84GSFNPCANPADD68 pKa = 3.66AWPIITEE75 pKa = 4.09NNISIILDD83 pKa = 3.81NPSMPCATDD92 pKa = 3.23NARR95 pKa = 11.84DD96 pKa = 3.79LFDD99 pKa = 5.03DD100 pKa = 4.5AGPNVGVAYY109 pKa = 9.96DD110 pKa = 3.76NPLRR114 pKa = 11.84AAMIVFLMMQVANNAA129 pKa = 3.49

Molecular weight: 13.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K7P720|K7P720_9CAUD NinB protein OS=Escherichia phage HK446 OX=1147145 GN=HK446_048 PE=4 SV=1
MM1 pKa = 6.89TKK3 pKa = 9.97RR4 pKa = 11.84RR5 pKa = 11.84KK6 pKa = 9.59KK7 pKa = 8.64YY8 pKa = 8.27QEE10 pKa = 3.91KK11 pKa = 10.86EE12 pKa = 4.34EE13 pKa = 4.08IRR15 pKa = 11.84HH16 pKa = 5.8PDD18 pKa = 3.24SPEE21 pKa = 3.86GLVVAAANNRR31 pKa = 11.84AFAEE35 pKa = 4.16RR36 pKa = 11.84LVGVYY41 pKa = 9.93RR42 pKa = 11.84LAKK45 pKa = 10.37AGVKK49 pKa = 9.75HH50 pKa = 5.82GRR52 pKa = 11.84RR53 pKa = 3.65

Molecular weight: 6.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

11855

32

1151

197.6

21.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.785 ± 0.528

1.223 ± 0.2

5.744 ± 0.251

6.183 ± 0.3

3.155 ± 0.17

7.491 ± 0.354

1.577 ± 0.237

5.812 ± 0.257

5.82 ± 0.404

7.777 ± 0.31

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.741 ± 0.148

4.833 ± 0.256

3.61 ± 0.282

4.682 ± 0.294

5.972 ± 0.31

6.892 ± 0.32

5.829 ± 0.329

6.428 ± 0.285

1.62 ± 0.157

2.826 ± 0.207
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski