Penicillium oxalicum (strain 114-2 / CGMCC 5302) (Penicillium decumbens)
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9977 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S8BDK4|S8BDK4_PENO1 Uncharacterized protein OS=Penicillium oxalicum (strain 114-2 / CGMCC 5302) OX=933388 GN=PDE_08054 PE=4 SV=1
MM1 pKa = 7.68 SYY3 pKa = 11.52 YY4 pKa = 10.64 NDD6 pKa = 3.44 SDD8 pKa = 3.87 NEE10 pKa = 4.17 RR11 pKa = 11.84 GGNQGPDD18 pKa = 2.7 SGYY21 pKa = 11.1 GGNGSYY27 pKa = 10.74 NADD30 pKa = 3.62 SNSNQDD36 pKa = 3.67 DD37 pKa = 3.79 FRR39 pKa = 11.84 GSSDD43 pKa = 3.02 TSGSDD48 pKa = 3.35 GQFRR52 pKa = 11.84 NSDD55 pKa = 3.61 SYY57 pKa = 11.93 GDD59 pKa = 3.46 QSGGFGGQNGRR70 pKa = 11.84 SDD72 pKa = 3.91 NNNNDD77 pKa = 3.61 DD78 pKa = 4.06 SYY80 pKa = 11.33 TNQSSEE86 pKa = 4.07 RR87 pKa = 11.84 SDD89 pKa = 3.69 NYY91 pKa = 10.99 GGNSGSNQGGNSGDD105 pKa = 3.53 SGFGGLVNQAANYY118 pKa = 8.55 LKK120 pKa = 10.5 QSSSSNNNGSNNSNSNSADD139 pKa = 3.32 NVVDD143 pKa = 3.86 SFVDD147 pKa = 3.54 KK148 pKa = 11.13 QVDD151 pKa = 3.57 QYY153 pKa = 11.91 APSGSDD159 pKa = 3.06 GFVNQGVNNVINNTMNKK176 pKa = 9.68 YY177 pKa = 10.09 LL178 pKa = 3.96
Molecular weight: 18.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.684
IPC2_protein 3.656
IPC_protein 3.681
Toseland 3.439
ProMoST 3.884
Dawson 3.706
Bjellqvist 3.872
Wikipedia 3.706
Rodwell 3.503
Grimsley 3.35
Solomon 3.694
Lehninger 3.656
Nozaki 3.834
DTASelect 4.164
Thurlkill 3.516
EMBOSS 3.706
Sillero 3.808
Patrickios 0.947
IPC_peptide 3.681
IPC2_peptide 3.783
IPC2.peptide.svr19 3.754
Protein with the highest isoelectric point:
>tr|S8ASB0|S8ASB0_PENO1 Uncharacterized protein OS=Penicillium oxalicum (strain 114-2 / CGMCC 5302) OX=933388 GN=PDE_03889 PE=4 SV=1
MM1 pKa = 7.26 FCIRR5 pKa = 11.84 CRR7 pKa = 11.84 ALPSIQSAASATRR20 pKa = 11.84 MPTTRR25 pKa = 11.84 ISQLTPRR32 pKa = 11.84 TLTTPAVSPSARR44 pKa = 11.84 FFSSTLLSTPTRR56 pKa = 11.84 ALVSNQPLFSRR67 pKa = 11.84 APSATTSSLPSIFSLLPPQTRR88 pKa = 11.84 AFSATAALGVKK99 pKa = 10.02 RR100 pKa = 11.84 NTFRR104 pKa = 11.84 PSRR107 pKa = 11.84 RR108 pKa = 11.84 VQKK111 pKa = 10.38 RR112 pKa = 11.84 RR113 pKa = 11.84 SGFLARR119 pKa = 11.84 NTSRR123 pKa = 11.84 KK124 pKa = 8.62 GRR126 pKa = 11.84 LVITRR131 pKa = 11.84 RR132 pKa = 11.84 RR133 pKa = 11.84 LKK135 pKa = 10.47 GRR137 pKa = 11.84 RR138 pKa = 11.84 AMSWW142 pKa = 2.72
Molecular weight: 15.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.481
IPC2_protein 11.052
IPC_protein 12.661
Toseland 12.822
ProMoST 13.32
Dawson 12.822
Bjellqvist 12.822
Wikipedia 13.305
Rodwell 12.398
Grimsley 12.866
Solomon 13.32
Lehninger 13.232
Nozaki 12.822
DTASelect 12.822
Thurlkill 12.822
EMBOSS 13.32
Sillero 12.822
Patrickios 12.12
IPC_peptide 13.334
IPC2_peptide 12.31
IPC2.peptide.svr19 9.143
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9977
0
9977
4542051
9
7877
455.3
50.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.643 ± 0.019
1.221 ± 0.01
5.603 ± 0.017
6.164 ± 0.024
3.686 ± 0.015
6.742 ± 0.029
2.502 ± 0.011
4.791 ± 0.016
4.549 ± 0.02
8.948 ± 0.031
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.23 ± 0.008
3.529 ± 0.012
6.123 ± 0.031
4.152 ± 0.016
6.329 ± 0.024
8.664 ± 0.03
5.894 ± 0.015
6.146 ± 0.017
1.439 ± 0.009
2.644 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here