Procyon lotor papillomavirus 1
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q4QW04|Q4QW04_9PAPI Protein E6 OS=Procyon lotor papillomavirus 1 OX=312349 GN=E6 PE=3 SV=1
MM1 pKa = 7.68 IGQGSSIGDD10 pKa = 3.3 IVLTEE15 pKa = 3.98 VPEE18 pKa = 5.56 AIDD21 pKa = 4.45 LYY23 pKa = 10.58 CDD25 pKa = 2.95 EE26 pKa = 5.42 HH27 pKa = 6.48 MPSDD31 pKa = 3.67 EE32 pKa = 4.28 EE33 pKa = 4.33 EE34 pKa = 4.4 EE35 pKa = 4.21 EE36 pKa = 4.28 EE37 pKa = 4.62 EE38 pKa = 4.13 PDD40 pKa = 3.2 EE41 pKa = 4.6 RR42 pKa = 11.84 EE43 pKa = 4.14 PFGVTVDD50 pKa = 3.63 CGVCKK55 pKa = 10.34 RR56 pKa = 11.84 RR57 pKa = 11.84 VNFVVLSDD65 pKa = 4.03 GEE67 pKa = 4.46 DD68 pKa = 3.03 IRR70 pKa = 11.84 RR71 pKa = 11.84 LQDD74 pKa = 3.59 LLFSLSIVCVSCVEE88 pKa = 4.35 SQSFQHH94 pKa = 6.64 GGG96 pKa = 3.09
Molecular weight: 10.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.998
IPC2_protein 3.986
IPC_protein 3.91
Toseland 3.732
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.757
Rodwell 3.745
Grimsley 3.643
Solomon 3.859
Lehninger 3.808
Nozaki 3.986
DTASelect 4.139
Thurlkill 3.77
EMBOSS 3.783
Sillero 4.024
Patrickios 2.842
IPC_peptide 3.859
IPC2_peptide 4.012
IPC2.peptide.svr19 3.915
Protein with the highest isoelectric point:
>tr|Q4QW02|Q4QW02_9PAPI Replication protein E1 OS=Procyon lotor papillomavirus 1 OX=312349 GN=E1 PE=3 SV=1
MM1 pKa = 7.25 EE2 pKa = 6.23 ALRR5 pKa = 11.84 QALDD9 pKa = 3.58 SVQEE13 pKa = 4.04 EE14 pKa = 4.55 LLNLYY19 pKa = 10.09 EE20 pKa = 5.34 KK21 pKa = 10.74 DD22 pKa = 3.82 SSDD25 pKa = 3.32 LTDD28 pKa = 5.71 QVTHH32 pKa = 6.14 WNLMRR37 pKa = 11.84 RR38 pKa = 11.84 EE39 pKa = 3.96 QVILHH44 pKa = 5.73 YY45 pKa = 10.51 ARR47 pKa = 11.84 KK48 pKa = 9.16 NAITRR53 pKa = 11.84 VGMTFVPPQNVSQQKK68 pKa = 9.38 AKK70 pKa = 10.5 EE71 pKa = 4.22 AIEE74 pKa = 3.96 QEE76 pKa = 4.82 LYY78 pKa = 10.66 LQSLLGSDD86 pKa = 4.03 YY87 pKa = 11.64 SNEE90 pKa = 3.61 RR91 pKa = 11.84 WTLSDD96 pKa = 3.01 TSRR99 pKa = 11.84 EE100 pKa = 4.01 VLLAPPMYY108 pKa = 9.96 CFKK111 pKa = 10.97 KK112 pKa = 10.02 GGRR115 pKa = 11.84 PVDD118 pKa = 3.37 VRR120 pKa = 11.84 FDD122 pKa = 3.81 GDD124 pKa = 3.92 PEE126 pKa = 4.46 NVTQYY131 pKa = 9.87 TLWGHH136 pKa = 7.11 IYY138 pKa = 9.3 YY139 pKa = 10.69 QNAEE143 pKa = 4.48 DD144 pKa = 3.59 VWQKK148 pKa = 9.81 TKK150 pKa = 10.79 GHH152 pKa = 6.1 VDD154 pKa = 3.36 DD155 pKa = 4.45 TGLYY159 pKa = 7.81 YY160 pKa = 10.12 TAEE163 pKa = 4.36 GEE165 pKa = 4.0 RR166 pKa = 11.84 VYY168 pKa = 11.29 YY169 pKa = 10.59 VVFKK173 pKa = 11.26 DD174 pKa = 3.25 EE175 pKa = 4.19 AKK177 pKa = 10.49 RR178 pKa = 11.84 YY179 pKa = 6.2 GTSGKK184 pKa = 10.55 YY185 pKa = 9.04 EE186 pKa = 4.04 VLHH189 pKa = 6.57 EE190 pKa = 4.05 LTQPVSTSTSPRR202 pKa = 11.84 PTGSTGNSTSLGPQPRR218 pKa = 11.84 TSTPRR223 pKa = 11.84 KK224 pKa = 9.64 KK225 pKa = 9.26 ITDD228 pKa = 3.01 RR229 pKa = 11.84 RR230 pKa = 11.84 RR231 pKa = 11.84 RR232 pKa = 11.84 RR233 pKa = 11.84 RR234 pKa = 11.84 VASLSKK240 pKa = 8.9 PTTVSTRR247 pKa = 11.84 GEE249 pKa = 3.87 QAPAPGLVAPTPEE262 pKa = 4.06 EE263 pKa = 3.76 VGRR266 pKa = 11.84 SHH268 pKa = 7.51 RR269 pKa = 11.84 SLGRR273 pKa = 11.84 RR274 pKa = 11.84 PGTRR278 pKa = 11.84 LEE280 pKa = 3.99 RR281 pKa = 11.84 LVLEE285 pKa = 4.66 ARR287 pKa = 11.84 DD288 pKa = 3.71 PPVVVFKK295 pKa = 11.23 GEE297 pKa = 4.15 PNSLKK302 pKa = 10.4 CLRR305 pKa = 11.84 FRR307 pKa = 11.84 LRR309 pKa = 11.84 KK310 pKa = 9.93 GYY312 pKa = 9.73 FHH314 pKa = 7.55 LFQKK318 pKa = 10.75 VSTTWSWTAGQGEE331 pKa = 4.36 RR332 pKa = 11.84 QGRR335 pKa = 11.84 ARR337 pKa = 11.84 MLMSFDD343 pKa = 3.83 SSEE346 pKa = 3.8 QRR348 pKa = 11.84 EE349 pKa = 4.5 SFLRR353 pKa = 11.84 QAPIPRR359 pKa = 11.84 SVQCFLGSLDD369 pKa = 4.35 DD370 pKa = 4.18 LL371 pKa = 4.73
Molecular weight: 42.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.861
IPC2_protein 8.902
IPC_protein 8.902
Toseland 9.385
ProMoST 9.268
Dawson 9.721
Bjellqvist 9.472
Wikipedia 9.926
Rodwell 9.867
Grimsley 9.823
Solomon 9.75
Lehninger 9.706
Nozaki 9.487
DTASelect 9.443
Thurlkill 9.545
EMBOSS 9.838
Sillero 9.648
Patrickios 4.94
IPC_peptide 9.75
IPC2_peptide 8.156
IPC2.peptide.svr19 7.818
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2334
96
600
333.4
37.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.955 ± 0.551
2.314 ± 0.709
6.341 ± 0.349
7.112 ± 0.71
4.37 ± 0.398
6.47 ± 0.53
1.842 ± 0.161
4.327 ± 0.774
4.67 ± 0.8
8.912 ± 0.567
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.5 ± 0.279
3.599 ± 1.012
7.027 ± 1.098
3.942 ± 0.381
7.412 ± 0.643
7.841 ± 0.987
5.527 ± 0.578
6.598 ± 0.591
1.414 ± 0.381
2.828 ± 0.39
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here